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Zhang B.,The Third Hospital of Liaocheng | Song C.,The Third Hospital of Liaocheng | Feng B.,The Third Hospital of Liaocheng | Fan W.,The Third Hospital Of Changsha
Therapeutics and Clinical Risk Management | Year: 2016

Triptolide, an active compound extracted from the Chinese herb thunder god vine (Tripterygium wilfordii Hook F.), has potent antitumor activity. Recently, triptolide was found to have protective effects against acute cerebral ischemia/reperfusion (I/R) injury through inhibition of cell apoptosis. However, the regulatory mechanism of the effect remains unclear. We hypothesize that the regulatory mechanisms of triptolide are mediated by nuclear factor κB (NF-κB) and p53-upregulated-modulator-of-apoptosis signal inhibition. To verify this hypothesis, we occluded the middle cerebral artery in male rats to establish focal cerebral I/R model. The rats received triptolide or vehicle at the onset of reperfusion following middle cerebral artery occlusion. At 24 hours after reperfusion, neurological deficits, infarct volume, and cell apoptosis were evaluated. The expression levels of NF-κBp65, PUMA, and caspase-3 were determined by Western blot. Real-time polymerase chain reaction was used to determine the levels of NF-κBp65 mRNA, PUMA mRNA, and caspase-3 mRNA. NF-κB activity was determined by electrophoretic mobility shift assay. Apoptotic cells were detected using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. In I/R group, neurological deficit scores, cerebral infarct volume, expression of NF-κBp65, PUMA, caspase-3, NF-κB activity, and TUNEL-positive cells were found to be increased at 24 hours after I/R injury. The I/R/triptolide rats showed significantly better neurological deficit scores, decreased neural apoptosis, and reduced cerebral infarct volume. In addition, the expression of NF-κBp65, PUMA, caspase-3, and NF-κB activity was suppressed in the I/R/triptolide rats. These results indicate that the neuroprotective effects of triptolide during acute cerebral I/R injury are possibly related to the inhibition of apoptosis through suppression of NF-κB/PUMA signaling pathway. © 2016 Zhang et al. Source


Fang C.,Central South University | Ren X.,Shanghai Green Valley Pharmaceutical Co. | Zhou H.,Central South University | Gong Z.-C.,Central South University | And 7 more authors.
Clinical and Experimental Pharmacology and Physiology | Year: 2014

The purpose of the present study was to clarify the association of eNOS 894G/T and ACE I/D genetic polymorphisms with the risk of coronary heart disease (CHD) and to explore the effects of these polymorphisms on the therapeutic efficacy of salvianolate injection in Chinese CHD patients. In all, 153 CHD patients and 198 healthy controls were enrolled in the study. We collected 5 mL peripheral blood for DNA extraction. Genetic diagnosis of eNOS 894G/T was determined by direct sequencing. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to detect ACE I/D genotypes. We observed significant differences in the frequency distribution of eNOS and ACE polymorphisms between CHD patients and healthy controls (P < 0.05). Binary logistic regression stepwise analysis revealed that the genotypes had an additive and dominant effect on patients' therapeutic responses (P < 0.05). These data suggest that the genetic polymorphisms of ACE I/D and eNOS 894G/T probably play a role in the development of CHD and these genetic polymorphisms may affect the response to salvianolate injection in Chinese CHD patients. © 2014 Wiley Publishing Asia Pty Ltd. Source


Pei Q.,Central South University | Zhang B.,Central South University | Tan H.,Central South University | Liu L.,The Third Hospital Of Changsha | And 7 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

Tolvaptan is a selective vasopressin V2-receptor antagonist mainly used for the treatment of hyponatremia. This study described the development and validation of an LC-MS/MS method for the determination of tolvaptan in human plasma. Sample preparation involved protein precipitation with acetonitrile containing 2-demethyl tolvaptan (internal standard, IS). Chromatographic separation was performed on a Zorbax XDB C18 column with an isocratic mobile phase consisting of water (containing 0.1% formic acid) and methanol (25:75, v/v). Determination of the analytes was achieved by tandem-mass spectrometry with positive electrospray ionization. The multiple reaction monitoring (MRM) transitions were performed at m/z 449.2→252.1 for tolvaptan and m/z 435.2→238.1 for IS. The assay was linear over the concentration range of 0.457-1000ng/mL, with a lower limit of quantification of 0.457ng/mL. The intra- and inter-day precisions at three concentration levels (0.914, 111 and 800ng/mL) were less than 15% and their accuracies were within the range of 97.7-107.8%. The mean recovery ranged from 99.2 to 104.6% and the matrix effect from 89.3 to 99.5%. Tolvaptan was stable under all tested conditions. This validated method was successfully applied to a pharmacokinetic study in healthy volunteers after oral administration of single-dose tolvaptan tablets. © 2012 Elsevier B.V. Source


Liang B.,Central South University | Yin Z.,Central South University | Guo Q.,The Third Hospital Of Changsha | Wei Y.,Central South University | And 2 more authors.
Experimental and Therapeutic Medicine | Year: 2013

Retroperitoneal fibrosis (RPF) is a rare disease of unclear etiology, which is characterized by a chronic non-specific inflammation of the retroperitoneum. The present study reports the case of a 36-year-old male with a 3-month history of lower right abdominal pain (intermittent) and weight loss (5 kg). A mass was identified that covered the surface of the abdominal aorta and the inferior vena cava near the right renal hilum. Three shots with an automated gun were employed to biopsy the mass. The patient began taking prednisone one month subsequent to the surgery at a dose of 10 mg, three times a day once a month and at continuously reducing doses for 1 year. CT scans showed that the retroperitoneal mass decreased in size with the progression of the treatment and that the mass had almost disappeared on the final month's MRI scan. In conclusion, the diagnosis of retroperitoneal fibrosis is an effective process that excludes the other diagnoses for the lesion. A biopsy of the mass is a necessity for the final stage of diagnosis and is supported by the response to the steroid treatment. Source


Wen W.,The Third Hospital Of Changsha | Deng L.,The Third Hospital Of Changsha
International Eye Science | Year: 2015

AIM: To investigate the risk factors of diabetic retinopathy (DR) in type 2 diabetes patients. METHODS: A total of 380 cases of type 2 diabetes were selected from January 2014 to June 2014, divided into DR group (126 cases) and non-diabetic retinopathy (NDR) as control group (254 cases), all patients were underwent medical history, physical examinations, laboratory and assistance examinations. The prevalence and single factor and multiple factors of DR were analysed by the logistic regression model. RESULTS: The univariate logistic regression analysis showed that DR was associated with diabetes duration, systolic blood pressure, triglyceride, total cholesterol, low-density lipoprotein, urine protein, intraocular pressure, carotid intima-media thickness, peripheral neuropathy disease was the related risk factors for DR. In multiple logistic regression analysis, only duration was the related risk factors for DR. CONCLUSION: The occurrence of DR is the outcome of combined action of multiple factors, duration is an independent risk factor for occurrence of DR. ©, 2015, International Journal of Ophthalmology (c/o Editorial Office). All right reserved. Source

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