Wang F.,Tianjin Medical University |
Jing X.,The Third Central Hospital of Tianjin |
Li G.,Tianjin University of Traditional Chinese Medicine |
Wang T.,Tianjin Medical University |
And 8 more authors.
Liver International | Year: 2012
Background: Recent studies have focused on regulatory T cells (Tregs) in chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC) and they were also conducted independently of each other. Aims: This study tried to characterize Tregs in blood and tumour infiltration, and to explore the correlations between Tregs and the context of chronic hepatitis B in HCC patients. Methods: The liver-resident Tregs and CD8 + T cells on core biopsy were investigated using immunohistochemistry staining in individuals (n = 209) with CHB (n = 47), HCC (n = 137) or healthy controls (n = 25). Circulating Tregs were detected in the above patients with CHB (n = 27) or HCC (n = 101) by flow cytometry. Results: The number of tumour-infiltrating and circulating FoxP3 + Tregs was significantly high in patients with CHB (P < 0.001). However, there were fewer intratumoural Tregs in patients with advanced HCC than those in patients with early stage HCC (P = 0.043); In contrast, the circulating Tregs frequency increased during the progression of HCC (P = 0.024). Increased tumour-infiltrating and circulating FoxP3 + Tregs were associated with poor overall survival (P = 0.041, 0.002 respectively) and a shorter time to recurrence (P = 0.049, 0.002 respectively) in patients with early stage HCC. Tumour-infiltrating Foxp3 + Tregs were related to chronic hepatitis B natural history in HCC (P = 0.012). Neither tumour-infiltrating CD8 + T cells nor balance of intratumoural Tregs and CD8 + T cells correlated with prognosis of HCC. Conclusions: Increased Foxp3 + Tregs may represent a prognostic predictor in patients with early stage HCC. The CHB natural history influenced density of tumour-infiltrating Tregs in hepatocellular carcinoma patients with chronic hepatitis B viruses infection. © 2011 John Wiley & Sons A/S.
PubMed | Chinese PLA General, Tianjin Medical University, The Third Central Hospital of Tianjin, The Affiliated Hospital of Taian Medical University and Tianjin Beichen Hospital
Type: | Journal: Biochemical and biophysical research communications | Year: 2017
Long noncoding RNA taurine-upregulated gene 1 (lncRNA TGU1) has been reported to play a key role in the progression of diabetic nephropathy (DN). However, the role of lncRNA TGU1 in the regulation of diabetic nephropathy remains largely unknown. The aim of the present study is to identify the regulation of lncRNA TGU1 on extracellular matrix accumulation via mediating microRNA-377 targeting of PPAR, and investigate the underlying mechanisms in progression of DN. Microarray was performed to screen differentially expressed miRNAs in db/db DN mice. Afterwards, computational prediction programs (TargetScan, miRanda, PicTar and miRGen) was applied to predict the target gene of miRNAs. The complementary binding of miRNA and lncRNA was assessed by luciferase assays. Protein and mRNA expression were detected by western blot and real time quantitate PCR. MiRNA-377 was screened by miRNA microarray and differentially up-regulated in db/db DN mice. PPAR was predicted to be the target of miR-377 and the prediction was verified by luciferase assays. Expression of miR-377 was up-regulated in mesangial cell treated with high glucose (25mM), and overexpression of miR-377 inhibited PPAR expression and promoted PAI-1 and TGF-1 expression. The expression of TGU1 antagonized the effect of miR-377 on the downregulation of its target PPAR and inhibited extracellular matrix accumulation, including PAI-1, TGF-1, fibronectin (FN) and collagen IV (Col IV), induced by high glucose. LncRNA TUG1 acts as an endogenous sponge of miR-377 and downregulates miR-377 expression levels, and thereby relieving the inhibition of its target gene PPAR and alleviates extracellular matrix accumulation of mesangial cells, which provides a novel insight of diabetic nephropathy pathogenesis.
PubMed | The Fourth Central Hospital of Tianjin and The Third Central Hospital of Tianjin
Type: Journal Article | Journal: PloS one | Year: 2015
Non-alcoholic fatty liver disease (NAFLD) is prevalent in individuals with type 2 diabetes mellitus (T2DM). Diabetic nephropathy (DN) is also associated with T2DM. However, little is known about the interaction between these conditions in patients with T2DM.To examine the association between NAFLD and DN in patients with T2DM.This retrospective study included patients seen between January 2006 and July 2014.T2DM patients were divided into two groups based on NAFLD status (with NAFLD = group A; without = group B). The cumulative incidence of DN and chronic kidney disease (CKD) staging were compared between the two groups. Liver fat content was examined in some patients. Associations among NAFLD, other factors,and DN were analyzed by the additive interaction method.Cumulative incidence of DN in patients from group A (58.58%) was higher than in group B (37.22%) (P = 0.005). In both groups, the number of DN patients with CKD stage 1 was greater than the number of patients with stages 2-5. Increased liver fat content was associated with increased occurrence of severe and mild albuminuria and decreased glomerular filtration rate (GFR). There were positive correlations between NAFLD and insulin resistance index (HOMA-IR), free fatty acids (FFA), tumor necrosis factor- (TNF-), omentin-1, visceral fat area, homocysteine (HCY), and serum uric acid (UA).NAFLD might be a risk factor for DN. Elevated liver fat content could be associated with higher DN burden.
Quantitative Proteomics Reveals That the Inhibition of Na(+)/K(+)-ATPase Activity Affects S-Phase Progression Leading to a Chromosome Segregation Disorder by Attenuating the Aurora A Function in Hepatocellular Carcinoma Cells
PubMed | Affiliated Hospital of Chinese Peoples Armed Police Force, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin University, The Third Central Hospital of Tianjin and Central University of Costa Rica
Type: Journal Article | Journal: Journal of proteome research | Year: 2015
Many studies have shown the Na(+)/K(+)-ATPase (NKA) might be a potential target for anticancer therapy. Cardiac glycosides (CGs), as a family of naturally compounds, inhibited the NKA activity. The present study investigates the antitumor effect of ouabain and elucidates the pharmacological mechanisms of CG activity in liver cancer HepG2 cell using SILAC coupled to LC-MS/MS method. Bioinformatics analysis of 330 proteins that were changed in cells under treatment with 0.5 mol/L ouabain showed that the biological processes are associated with an acute inflammatory response, cell cycle, oxidation reduction, chromosome segregation, and DNA metabolism. We confirmed that ouabain induced chromosome segregation disorder and S-cell cycle block by decreasing the expression of AURKA, SMC2, Cyclin D, and p-CDK1 as well as increasing the expression of p53. We found that the overexpression or inhibition of AURKA significantly reduced or enhanced the ouabain-mediated the anticancer effects. Our findings suggest that AURKA is involved in the anticancer mechanisms of ouabain in HepG2 cells.
PubMed | RAS N. D. Zelinsky Institute of Organic Chemistry, The Third Central Hospital of Tianjin and Nankai University
Type: | Journal: Carbohydrate research | Year: 2016
The O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Escherichia coli O137 and studied by sugar analysis and NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established: Formula: see text] Both structure and gene cluster of the E. coli O137 polysaccharide are related to those of the E. coli K40 polysaccharide (Amor et al., 1999), which lacks the side-chain glucosylation but contains serine that is amide-linked to GlcA. Functions of genes in the O137-antigen gene cluster were assigned by a comparison with those in K40 and sequences in the available databases. Particularly, predicted glycosyltransferases encoded in the gene cluster were assigned to the formation of three glycosidic linkages in the O-polysaccharide repeating unit.
Wang J.,Tianjin Medical University |
Zhu Z.,The Third Central Hospital of Tianjin |
Huang Y.,Tianjin Medical University |
Wang P.,The Third Central Hospital of Tianjin |
And 3 more authors.
Biotechnology Letters | Year: 2014
Human placental mesenchymal stem cells (hPMSCs), for the treatment of fulminant hepatic failure, have been widely studied. Only a few studies have investigated the effect of the subtype CD200+hPMSCs on regeneration of human hepatocytes. CD200+hPMSCs can down-regulate activity of several immunocytes and suppress TNF-α secretion from macrophages via the CD200-CD200R axis. We have investigated the influence of CD200-positive human placenta chorionic mesenchymal stem cells (CD200+hPCMSCs) on metabolism, proliferation and apoptosis of human hepatocytes in vitro. CD200+hPCMSCs promote urea synthesis, albumin secretion and hepatocytes proliferation at co-culture ratios of 1:1 and 3:1. Additionally, CD200+hPCMSCs inhibit hepatocyte apoptosis via up-regulation of an anti-apoptotic protein, Bcl-xL. Thus, CD200+hPCMSCs can provide supportive benefit for the regeneration of human hepatocytes and also have immunosuppressive properties. Therefore, CD200+hPCMSCs may be an ideal candidate for stem cell-based therapy in hepatic failure. © 2014 Springer Science+Business Media Dordrecht.
Wen X.-N.,The Third Central Hospital of Tianjin
Chinese Journal of New Drugs | Year: 2012
Objective: To investigate the clinical characteristics and causes of clindamycin-induced allotriogeusia from clinical practice, and to provide reference for rational drug use in clinic. Methods: One case of allotriogeusia occurred in our hospital was reported, which was possibly induced by clindamycin. Literature of clindamycin-induced allotriogeusia in domestic pharmaceutical journals from 1994 to 2011 was collected and analyzed statistically. Results: The clinical information of dosage regimen, onset time, treatment methods and efficacy in 12 patients with allotriogeusia reported in our hospital and literature were analyzed. Clindamycin-induced allotriogeusia occurred following oral administration, intramuscular injection and intravenous infusion; the onset time was within 10 minutes to 2 days after medication. In 9 patients, the administration intervals were once daily; in 5 patients, the single doses were too large, which did not comply with the dosage of the drug instructions. The prognosis was better after withdrawal or replacement of the drugs. Conclusion: Clindamycin-induced allotriogeusia seems to correlate to the dosage and dosing frequency. More attention should be paid to clindamycin-induced allotriogeusia, and clinical application should comply with the usage and dosage to ensure the safety of drug use.
Zhang J.,The Third Central Hospital of Tianjin |
Li Q.,The Third Central Hospital of Tianjin |
Zhang Z.,The Third Central Hospital of Tianjin |
Sun X.,The Third Central Hospital of Tianjin
Neurochirurgie | Year: 2015
The authors report an unusual case of diffuse subarachnoid hemorrhage on brain computed tomography (CT) scan in a patient with post-resuscitation anoxic encephalopathy. A 42-year-old woman suffered both respiratory and cardiac arrest, associated with hypoxic encephalopathy, which occurred during a visit to our gynecology clinic. CT examination was performed the next day, which revealed a hyperdensity in the basal cisterns with a diffuse cerebral edema. Lumbar puncture was applied for diagnosis. No yellow coloration or red cells were observed in the cerebrospinal fluid. Nineteen days after treatment, the CT examination revealed features of a subarachnoid hemorrhage with a significantly increased cerebral edema. The patient died two months later. This clinical case illustrates that hypoxic encephalopathy can mimic diffuse subarachnoid hemorrhage on CT scan. © 2015.
Cai J.,Tianjin Medical University |
Han T.,Tianjin Medical University |
Han T.,The Third Central Hospital of Tianjin |
Nie C.,Tianjin Medical University |
And 4 more authors.
Clinics and Research in Hepatology and Gastroenterology | Year: 2016
Background and objective: Hepatitis B virus related acute-on-chronic liver failure is a serious condition with a high mortality. Oxidative stress, inflammation, necrosis and apoptosis may play an important role in it. This study is to investigate whether serum AOPP, S100A12, HMGB1 and sRAGE can provide diagnostic or prognostic information in HBV-related ACLF. Methods: We measured serum S100A12, HMGB1 and sRAGE levels in 50 patients with HBV-related ACLF, 35 patients with liver cirrhosis (LC), 35 patients with chronic hepatitis B (CHB) and 35 healthy controls by enzyme-linked immunosorbent assay. AOPP measured by spectrophotometry. Results: Significantly higher AOPP, S100A12, HMGB1 and sRAGE levels on admission were found in patients with ACLF compared with LC, CHB and healthy controls (P < 0.001). In ACLF patients, they were higher in nonsurvivors than survivors (P < 0.001). They had a positive relationship with total bilirubin and MELD scores. AOPP, S100A12 and HMGB1 concentrations continually declined in survivors while increased in nonsurvivors, sRAGE concentrations did not change in survivors, but gradually increased in nonsurvivors during hospitalization. ROC curve analysis showed that the four biomarkers had a higher AUC than TBIL. Multivariate Cox regression analysis demonstrated that S100A12, AOPP and sRAGE were independent risk factors for poor prognosis. Conclusion: Serum AOPP, S100A12 and sRAGE maybe reflect the oxidation stress, inflammation levels in HBV-related acute-on-chronic liver failure. Increased AOPP, S100A12 and sRAGE may serve as important biological markers of worse outcome. © 2015 Elsevier Masson SAS.
PubMed | Tianjin Medical University and The Third Central Hospital of Tianjin
Type: Journal Article | Journal: Clinics and research in hepatology and gastroenterology | Year: 2016
Hepatitis B virus related acute-on-chronic liver failure is a serious condition with a high mortality. Oxidative stress, inflammation, necrosis and apoptosis may play an important role in it. This study is to investigate whether serum AOPP, S100A12, HMGB1 and sRAGE can provide diagnostic or prognostic information in HBV-related ACLF.We measured serum S100A12, HMGB1 and sRAGE levels in 50 patients with HBV-related ACLF, 35 patients with liver cirrhosis (LC), 35 patients with chronic hepatitis B (CHB) and 35 healthy controls by enzyme-linked immunosorbent assay. AOPP measured by spectrophotometry.Significantly higher AOPP, S100A12, HMGB1 and sRAGE levels on admission were found in patients with ACLF compared with LC, CHB and healthy controls (P<0.001). In ACLF patients, they were higher in nonsurvivors than survivors (P<0.001). They had a positive relationship with total bilirubin and MELD scores. AOPP, S100A12 and HMGB1 concentrations continually declined in survivors while increased in nonsurvivors, sRAGE concentrations did not change in survivors, but gradually increased in nonsurvivors during hospitalization. ROC curve analysis showed that the four biomarkers had a higher AUC than TBIL. Multivariate Cox regression analysis demonstrated that S100A12, AOPP and sRAGE were independent risk factors for poor prognosis.Serum AOPP, S100A12 and sRAGE maybe reflect the oxidation stress, inflammation levels in HBV-related acute-on-chronic liver failure. Increased AOPP, S100A12 and sRAGE may serve as important biological markers of worse outcome.