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Ma Z.,The Third Affiliated Hospital of Zunyi Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

By nearly 3-year retrospective analysis of cases with space-occupying lesions in unilateral nasal sinus in Guizhou Province People's Hospital, clinical diagnostic and misdiagnosis of such lesions were explored to provide references for clinicians in diagnosis and treatment of such diseases. Combining related literatures in recent years, 213 patients with space-occupying lesions in unilateral nasal sinus were selected. The patients misdiagnosed were reviewed for its clinical manifestations, of patients had been misdiagnosed, imaging features and and pathology. Of 213 patients, 116 cases located in the left nasal sinus and 97 in right, 65 patients were nasal polyps, 66 were sinus cyst, 20 were fungal sinusitis, 31 were benign tumor, 28 were malignant tumor and 3 were nasal foreign body. Misdiagnosis were as follow: 12 patients with malignant tumor were misdiagnosed as nasal polyps and the misdiagnosis rate 5.63%. Nasal foreign bodies were misdiagnosed as sinusitis in 2 cases and the misdiagnosis rate 0. 94%. Inserted papilloma misdiagnosed as nasal polyps in 6 cases and the misdiagnosis rate 8.45%. Fungal sinusitis misdiagnosed as purulent sinusitis in 5 cases and the misdiagnosis rate 2.35%. Sinus cyst misdiagnosed as sinusitis in 8 cases and the misdiagnosis rate 3.75%. Enquiry history, carefully specialized examination including nasal endoscopy, three-dimensional image and biopsy are crucial on the accurate diagnosis and reducing the misdiagnosis. Source


Zhou J.,The Affiliated Hospital of Jining Medical College | Zhou J.,Zunyi Medical College | Ma R.,Zunyi Medical College | Luo R.,Zunyi Medical College | And 6 more authors.
Cancer Epidemiology | Year: 2010

Background: The immunoscope spectratyping to TRBV CDR3 had been applied in infectious diseases, tumors, autoimmune diseases and so on, this study aimed to primarily explore CDR3 spectratyping and molecular features of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma. Methods: Blood and tissue samples were collected from seven patients with colorectal carcinoma (CRC), while blood samples were also collected from two healthy controls as control. Using the real-time fluorescence quantitative reverse transcription polymerase chain reaction (RQ-PCR) and DNA melting curve analysis techniques, the features of T-cell receptor beta chain variable region (TRBV) were determined. Results: The gene melting spectral pattern (GMSP) of 24 TRBV gene families exhibited a highly diverse multimodal shape for most of the TRBV gene families, compared to healthy controls, the more GMSP of patients with CRC showed either a single peak, or several prominent melting peaks (skewed) for certain TRBV gene families. Different patients have different skewed patterns. In the analysis results of sequence, some TRBV CDR3 gene families showed sharing the same motif, such as, TRBV6 of P5, TRBV13.1 of P6 and TRBV21 of P7 (tissue sample) shared the same motif 'GT'; TRBV1 of P1 and TRBV21 of P7 shared the same motif 'AGG', TRBV11 of P1, TRBV21 of P5 and TRBV21 of P7 (tissue sample) shared the same motif 'TDTQY', and even TRBV21 of P5 and P7 (tissue sample) shared the large motif 'SGTDTQY'. As a whole, most of TRBV gene families have the similar motifs 'X-Q', the nucleotide 'X' mainly was 'E', it also was possible be 'T', 'G' or 'K' in some CDR3 gene families of patients with CRC. Conclusions: There were different GMSPs in different patients with CRC, CDR3 spectratyping and the molecular features of TCR β chain in the peripheral blood and tissue of patients with CRC were not same or similar, this information would provide ideas for individualized therapy to CRC. © 2010. Source


Xu H.,Chongqing Medical University | Wang L.,Chongqing Medical University | Huang J.,Chongqing Medical University | Zhang Y.,Chongqing Medical University | And 7 more authors.
Journal of Microbiology | Year: 2015

Pneumococcal asymptomatic colonization of the respiratory tracts is a major risk for invasive pneumococcal disease. We have previously shown that pneumococcal wall teichoic acid (WTA) was involved in pneumococcal infection of sepsis and adherence to epithelial and endothelial cells. In this study, we investigated the contribution of pneumococcal WTA to bacterial colonization and dissemination in murine models. The result showed that nasopharynx colonizing D39 bacterial cells have a distinct phenotype showing an increased exposure of teichoic acids relative to medium-grown bacteria. The WTA-deficient mutants were impaired in their colonization to the nasopharynx and lungs, and led to a mild inflammation in the lungs at 36 h post-inoculation. Pretreatment of the murine nares with WTA reduced the ability of wild type D39 bacteria to colonize the nasopharynx. In addition, the WTA-deficient strain was impaired in its ability to invade the blood and brain following intranasal administration. WTA-deficient D39 strain was reduced in C3 deposition but was more susceptible to the killing by the neutrophils as compared with its parent strain. Our results also demonstrated that the WTA enhanced pneumococcal colonization and dissemination independently of the host strains. These results indicate that WTA plays an important role in pneumococcal pathogenesis, both in colonization and dissemination processes. © 2015, The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg. Source


Zhou J.-W.,Zunyi Medical College | Zhou J.-W.,Affiliated Hospital of Jining Medical College | Ma R.,Affiliated Hospital of Jining Medical College | Tang W.-T.,The Third Affiliated Hospital of Zunyi Medical College | And 4 more authors.
Acta Medica Mediterranea | Year: 2011

Objective: To explore and compare the skewing features of T-cell receptor (TCR) alpha chain complementarity determining region 3 (CDR3) between in peripheral blood and in colon tissue of patients with colorectal carcinoma (CRC). Methods: Using real-time fluorescence quantitative reverse transcription polymerase chain reaction (FQ-PCR) with DNA melting curve technique, the features of TRAV CDR3 were determined. Results: The gene melting spectral patterns (GMSPs) of 32 TRAV gene families exhibited highly multimodal shapes for most of the TRAV gene families, and comparing with healthy controls and tissue sample controls with lymphnoditis respectively, many GMSPs of PBMC and TIL in patients with CRC showed skewed-peak (single-peak, biaed-peak and low-peak). However, some TRAV gene families which exhibited predomiant usage or limited usage were shared in PBMC and TIL, and there were similar or same sequences in some TRAV gene families which exhibited single-peak in PBMC or in TIL, some of families shared the same motifs. Conclusions: The skewing of TRAV in PBMC was different from that in TIL in most of patients with CRC, this information may be suggestive to the study of the immunological mechanism of CRC and the individualized therapy to it. Source


Yang L.,The Third Affiliated Hospital of Zunyi Medical College | Yang L.,Guizhou Provincial Cardiovascular Institute | Guan H.,Peking Union Medical College | He J.,The Third Affiliated Hospital of Zunyi Medical College | And 7 more authors.
Cell Biology International | Year: 2012

We have investigated whether VEGF (vascular endothelial growth factor) regulates the proliferative capacity and eNOS (endothelial nitric oxide synthase)/NO (nitric oxide) pathway of EPCs (endothelial progenitor cells) by activating CaN (calcineurin)/NFAT (nuclear factor of activated T-cells) signalling. EPCs were obtained from cultured mononuclear cells isolated from the peripheral blood of healthy adults. Treatment with VEGF (50 ng/ml) potently promoted CaN enzymatic activity, activation of NFAT2, cell proliferation, eNOS protein expression and NO production. Pretreatment with cyclosporin A (10 mg/ml), a pharmacological inhibitor of CaN or 11R-VIVIT, a special inhibitor of NFAT, completely abrogated the aforementioned effects of VEGF treatment and increased apoptosis. The results indicate that VEGF treatment promotes the proliferative capacity of human EPCs by activating CaN/NFAT signalling leading to increased eNOS protein expression and NO production. © The Author(s) Journal compilation © 2012 Portland Press Limited. Source

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