The Third Affiliated Hospital of Zunyi Medical College

Zunyi, China

The Third Affiliated Hospital of Zunyi Medical College

Zunyi, China
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Yu Y.,The Third Affiliated Hospital of Zunyi Medical College | Cao F.,The First Affiliated Hospital of Zunyi Medical College | Ran Q.,The First Affiliated Hospital of Zunyi Medical College | Wang F.,The Third Affiliated Hospital of Zunyi Medical College
Biochemical and Biophysical Research Communications | Year: 2017

Traumatic brain injury (TBI) remains a life-threatening disease. Accumulating evidences have showed that neuroinflammatory response is a critical biological event in the progression of TBI induced astrocyte damage. However, the exact mechanisms are not well understood. In this study, we demonstrated that long non-coding RNA (lncRNA) Gm4419 promoted trauma-induced astrocyte apoptosis by up-regulating the expression of inflammatory cytokine tumor necrosis factor α (TNF-α). We observed that Gm4419 was aberrantly induced after injury on astroglial cells in vitro. Overexpression of Gm4419 in injury-treated astrocytes increased protein expressions of TNF-α, Bax, cleaved caspase-3 and cleaved caspase-9, decreased levels of Bcl-2 and CyclinD1, and significantly led to cellular apoptosis. Mechanically, Gm4419 transcript could function as a sponge for miR-466l and miR-466l could target TNF-α 3' UTR for degradation and translation inhibition. Therefore, Gm4419 could up-regulate TNF-α expression by competitively binding miR-466l and then contribute to inflammatory damage as well as astrocyte apoptosis during TBI. Generally speaking, our findings provide better understandings of the mechanism underlying Gm4419 in trauma-induced neuroinflammation and neurological deficits. Thus, the present study would expand the insight into the novel approaches for TBI therapy. © 2017 Elsevier Inc.


Yao S.-T.,The First Affiliated Hospital of Zunyi Medical College | Cao F.,The First Affiliated Hospital of Zunyi Medical College | Chen J.-L.,The Third Affiliated Hospital of Zunyi Medical College | Chen W.,The First Affiliated Hospital of Zunyi Medical College | And 7 more authors.
Journal of Molecular Neuroscience | Year: 2017

Complement-mediated inflammation plays a vital role in intracerebral hemorrhage (ICH), implicating pro-inflammatory factor interleukin-1beta (IL-1β) secretion. Brain samples and contralateral hemiencephalon were all collected and detected by Western blot. NLRP3 expression was located by dual immunofluorescence staining at 1, 3, and 5 days post-ICH. Brain water content was examined post-ICH. The neural deficit scores were evaluated by observers blindly. ILs were detected by ELISA. SiRNAs targeting NLRP3 (siNLRP3), siASC, and siControl were injected to inhibit NLRP3 function. To test the complement activation via Nod-like receptor (NLR) family pyrin domain-containing 3 (NLRP3), normal rabbit complement (NRC) was injected with lipopolysaccharide (LPS) to facilitate the complement function. As a result, complement 3a (C3a) and complement 5a (C5a) were upregulated during the ICH-induced neuroinflammation, and ablation of C3 attenuates ICH-induced IL-1β release. Though the LPS rescues the neuroinflammation in the ICH model, C3 deficiency attenuates the LPS-induced inflammatory effect. The NLRP3 inflammasome was activated after ICH and was located in the microglial cell of the mouse brain, which exhibits a time-dependent manner. However, the number of NLRP3/Iba-1 dual-labeled cells in the C3−/− group is less than that in the WT group in each time course, respectively. IL-1β and IL-18 released in perihematoma tissue, caspase-1-p20, brain water content, and behavioral outcomes were attenuated in the siNLRP3 and siASC groups than in the siControl and ICH groups. We also found that 5% of complement supplement enhances ICH-induced IL-1β release, while NLRP3 and ASC inhibition attenuates it. In conclusion, complement-induced ICH neuroinflammation depended on NLRP3 activation, which facilities LPS- and ICH-induced neuroinflammation, and NLRP3 is required for ICH-induced inflammation. © 2016, Springer Science+Business Media New York.


Li X.-M.,The Third Affiliated Hospital of Zunyi Medical College | Liu X.-Y.,The Third Affiliated Hospital of Zunyi Medical College | Liu Z.-X.,The Third Affiliated Hospital of Zunyi Medical College
Oncology Letters | Year: 2015

Gestational trophoblastic disease with a primary extra-uterine nidus is rare, particularly during the postmenopausal period. The present report outlines a case of high-risk choriocarcinoma (International Federation of Gynecology and Obstetrics stage IV; World Health Organization score 13) in a 68-year-old female exhibiting neoplasm. The choriocarcinoma developed 20 years subsequent to the onset of menopause and 42 years following the patient's final pregnancy, and was associated with multiple metastases to the lungs, skull, neck, lymph nodes and skin. The patient was administered two courses of systemic chemotherapy with tegafur (800 mg) and actinomycin D (200 μg). Local chemotherapy was also administered to the scalp and left flank masses; the masses were injected first with methotrexate, and then with 5-fluorouracil. During chemotherapy, the patient's levels of β-human chorionic gonadotrophin (β-HCG) decreased from 3,171 IU/l to 1,763 IU/l, however by the conclusion of the courses of systemic and local chemotherapy, β-HCG levels had increased to 3,704 IU/l. The patient and their family subsequently elected to end treatment. The patient subsequently succumbed to infection, tumor consumption and organ insufficiency. This study describes the clinical and radiological features, as well as the treatment used for this rare type of choriocarcinoma. © 2015, Spandidos Publications. All rights reserved.


Ma Z.,the Third Affiliated Hospital of Zunyi Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

By nearly 3-year retrospective analysis of cases with space-occupying lesions in unilateral nasal sinus in Guizhou Province People's Hospital, clinical diagnostic and misdiagnosis of such lesions were explored to provide references for clinicians in diagnosis and treatment of such diseases. Combining related literatures in recent years, 213 patients with space-occupying lesions in unilateral nasal sinus were selected. The patients misdiagnosed were reviewed for its clinical manifestations, of patients had been misdiagnosed, imaging features and and pathology. Of 213 patients, 116 cases located in the left nasal sinus and 97 in right, 65 patients were nasal polyps, 66 were sinus cyst, 20 were fungal sinusitis, 31 were benign tumor, 28 were malignant tumor and 3 were nasal foreign body. Misdiagnosis were as follow: 12 patients with malignant tumor were misdiagnosed as nasal polyps and the misdiagnosis rate 5.63%. Nasal foreign bodies were misdiagnosed as sinusitis in 2 cases and the misdiagnosis rate 0. 94%. Inserted papilloma misdiagnosed as nasal polyps in 6 cases and the misdiagnosis rate 8.45%. Fungal sinusitis misdiagnosed as purulent sinusitis in 5 cases and the misdiagnosis rate 2.35%. Sinus cyst misdiagnosed as sinusitis in 8 cases and the misdiagnosis rate 3.75%. Enquiry history, carefully specialized examination including nasal endoscopy, three-dimensional image and biopsy are crucial on the accurate diagnosis and reducing the misdiagnosis.


Zhang W.,The Third Affiliated Hospital of Zunyi Medical College | Huang B.,The Third Affiliated Hospital of Zunyi Medical College | She C.,The Third Affiliated Hospital of Zunyi Medical College | Liu Y.,The Third Affiliated Hospital of Zunyi Medical College | And 3 more authors.
Saudi Medical Journal | Year: 2015

Objectives: To re-evaluate the epidemiology of hand, foot, and mouth disease (HFMD) in a non-vaccinated population in Zunyi, China. Methods: We used laboratory-based data from the Third Affiliated Hospital of Zunyi Medical College, Zunyi, China to assess the epidemiology of the HFMD caused by enteroviruses between January 2012 and November 2014. Real-time polymerase chain reaction was used to determine human enteroviruses from a total of 12313 probable cases enrolled in this retrospective study. All analyses were stratified by enterovirus serotype, gender, and age. Results: Virological results were available for 12313 cases of probable HFMD. A total of 5750 cases were positive for viral detection, and the positive rates of infection caused by other enteroviruses was 46.7%, EV71 9%, and CVA16 4.7%. During the study period there was a substantial increase in the occurrence of HFMD. Most of the HFMD patients (87.4%) were aged 0-59 months (median 24; range 0-59). Males showed a higher HFMD prevalence rate (62.4%) than females (37.6%). Conclusion: Enterovirus infection remains an important public health problem and other entroviruses are emerging as the major causative agent of the HFMD in Zunyi, China. © 2015, Saudi Arabian Armed Forces Hospital. All rights reserved.


Chen W.,The Third Affiliated Hospital of Zunyi Medical College | Feng C.-L.,The Third Affiliated Hospital of Zunyi Medical College | Zhu H.-T.,The Third Affiliated Hospital of Zunyi Medical College
World Chinese Journal of Digestology | Year: 2015

AIM: To assess the effects of different procedures of pancreaticojejunostomy selected based on pancreatic duct diameter and other factors on postoperative recovery.METHODS: A retrospective analysis was performed of the clinical data for 108 patients who underwent pancreaticojejunostomy at the Third Affiliated Hospital of Zunyi Medical College from January 2010 to January 2014. For patients with a pancreatic duct diameter ≥ 3 mm (n = 42), pancreaticojejunostomy was adopted (group A). There were 66 patients with a duct diameter < 3 mm, of whom 28 had a relatively thick pancreatic stump larger than the diameter of the jejunum and received the modified Child pancreaticojejunostomy (group B), and the remaining 38 with a pancreatic stump diameter smaller than the diameter of the jejunum received binding pancreaticojejunostomy (group C). The rate of postoperative complications and clinical efficacy were compared for the two groups.RESULTS: The pancreatic duct diameter was significantly larger in group A than in groups B and C (P < 0.05), while the pancreatic stump was the largest in group B, significantly larger than the other two groups (P < 0.05). The total time of surgery was not statistically significant among the three groups (P > 0.05). There were nine cases of pancreatic fistula in all of the patients, and the total rate of pancreatic fistula was 8.33%. The rates of postoperative abdominal bleeding, pancreatic fistula, digestive dysfunction, abdominal infection, death, and the average length of stay were not statistically significant among the three groups (P > 0.05).CONCLUSION: Selection of pancreaticojejunostomy based on the patient's pancreatic duct diameter, jejunum diameter and the diameter of the pancreatic stump is a reasonable way. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Xu H.,Chongqing Medical University | Wang L.,Chongqing Medical University | Huang J.,Chongqing Medical University | Zhang Y.,Chongqing Medical University | And 7 more authors.
Journal of Microbiology | Year: 2015

Pneumococcal asymptomatic colonization of the respiratory tracts is a major risk for invasive pneumococcal disease. We have previously shown that pneumococcal wall teichoic acid (WTA) was involved in pneumococcal infection of sepsis and adherence to epithelial and endothelial cells. In this study, we investigated the contribution of pneumococcal WTA to bacterial colonization and dissemination in murine models. The result showed that nasopharynx colonizing D39 bacterial cells have a distinct phenotype showing an increased exposure of teichoic acids relative to medium-grown bacteria. The WTA-deficient mutants were impaired in their colonization to the nasopharynx and lungs, and led to a mild inflammation in the lungs at 36 h post-inoculation. Pretreatment of the murine nares with WTA reduced the ability of wild type D39 bacteria to colonize the nasopharynx. In addition, the WTA-deficient strain was impaired in its ability to invade the blood and brain following intranasal administration. WTA-deficient D39 strain was reduced in C3 deposition but was more susceptible to the killing by the neutrophils as compared with its parent strain. Our results also demonstrated that the WTA enhanced pneumococcal colonization and dissemination independently of the host strains. These results indicate that WTA plays an important role in pneumococcal pathogenesis, both in colonization and dissemination processes. © 2015, The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.


Zhou J.,The Affiliated Hospital of Jining Medical College | Zhou J.,Zunyi Medical College | Ma R.,Zunyi Medical College | Luo R.,Zunyi Medical College | And 6 more authors.
Cancer Epidemiology | Year: 2010

Background: The immunoscope spectratyping to TRBV CDR3 had been applied in infectious diseases, tumors, autoimmune diseases and so on, this study aimed to primarily explore CDR3 spectratyping and molecular features of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma. Methods: Blood and tissue samples were collected from seven patients with colorectal carcinoma (CRC), while blood samples were also collected from two healthy controls as control. Using the real-time fluorescence quantitative reverse transcription polymerase chain reaction (RQ-PCR) and DNA melting curve analysis techniques, the features of T-cell receptor beta chain variable region (TRBV) were determined. Results: The gene melting spectral pattern (GMSP) of 24 TRBV gene families exhibited a highly diverse multimodal shape for most of the TRBV gene families, compared to healthy controls, the more GMSP of patients with CRC showed either a single peak, or several prominent melting peaks (skewed) for certain TRBV gene families. Different patients have different skewed patterns. In the analysis results of sequence, some TRBV CDR3 gene families showed sharing the same motif, such as, TRBV6 of P5, TRBV13.1 of P6 and TRBV21 of P7 (tissue sample) shared the same motif 'GT'; TRBV1 of P1 and TRBV21 of P7 shared the same motif 'AGG', TRBV11 of P1, TRBV21 of P5 and TRBV21 of P7 (tissue sample) shared the same motif 'TDTQY', and even TRBV21 of P5 and P7 (tissue sample) shared the large motif 'SGTDTQY'. As a whole, most of TRBV gene families have the similar motifs 'X-Q', the nucleotide 'X' mainly was 'E', it also was possible be 'T', 'G' or 'K' in some CDR3 gene families of patients with CRC. Conclusions: There were different GMSPs in different patients with CRC, CDR3 spectratyping and the molecular features of TCR β chain in the peripheral blood and tissue of patients with CRC were not same or similar, this information would provide ideas for individualized therapy to CRC. © 2010.


PubMed | The Third Affiliated Hospital of Zunyi Medical College and The First Affiliated Hospital of Zunyi Medical College
Type: | Journal: Journal of molecular neuroscience : MN | Year: 2016

Complement-mediated inflammation plays a vital role in intracerebral hemorrhage (ICH), implicating pro-inflammatory factor interleukin-1beta (IL-1) secretion. Brain samples and contralateral hemiencephalon were all collected and detected by Western blot. NLRP3 expression was located by dual immunofluorescence staining at 1, 3, and 5days post-ICH. Brain water content was examined post-ICH. The neural deficit scores were evaluated by observers blindly. ILs were detected by ELISA. SiRNAs targeting NLRP3 (siNLRP3), siASC, and siControl were injected to inhibit NLRP3 function. To test the complement activation via Nod-like receptor (NLR) family pyrin domain-containing 3 (NLRP3), normal rabbit complement (NRC) was injected with lipopolysaccharide (LPS) to facilitate the complement function. As a result, complement 3a (C3a) and complement 5a (C5a) were upregulated during the ICH-induced neuroinflammation, and ablation of C3 attenuates ICH-induced IL-1 release. Though the LPS rescues the neuroinflammation in the ICH model, C3 deficiency attenuates the LPS-induced inflammatory effect. The NLRP3 inflammasome was activated after ICH and was located in the microglial cell of the mouse brain, which exhibits a time-dependent manner. However, the number of NLRP3/Iba-1 dual-labeled cells in the C3


PubMed | the Third Affiliated Hospital of Zunyi Medical College
Type: Journal Article | Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

By nearly 3-year retrospective analysis of cases with space-occupying lesions in unilateral nasal sinus in Guizhou Province Peoples Hospital, clinical diagnostic and misdiagnosis of such lesions were explored to provide references for clinicians in diagnosis and treatment of such diseases.Combining related literatures in recent years, 213 patients with space-occupying lesions in unilateral nasal sinus were selected. The patients misdiagnosed were reviewed for its clinical manifestations, of patients had been misdiagnosed, imaging features and and pathology.Of 213 patients, 116 cases located in the left nasal sinus and 97 in right, 65 patients were nasal polyps, 66 were sinus cyst, 20 were fungal sinusitis, 31 were benign tumor, 28 were malignant tumor and 3 were nasal foreign body. Misdiagnosis were as follow: 12 patients with malignant tumor were misdiagnosed as nasal polyps and the misdiagnosis rate 5.63%. Nasal foreign bodies were misdiagnosed as sinusitis in 2 cases and the misdiagnosis rate 0. 94%. Inserted papilloma misdiagnosed as nasal polyps in 6 cases and the misdiagnosis rate 8.45%. Fungal sinusitis misdiagnosed as purulent sinusitis in 5 cases and the misdiagnosis rate 2.35%. Sinus cyst misdiagnosed as sinusitis in 8 cases and the misdiagnosis rate 3.75%.Enquiry history, carefully specialized examination including nasal endoscopy, three-dimensional image and biopsy are crucial on the accurate diagnosis and reducing the misdiagnosis.

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