Xingyuan S.,The Third Affiliated Hospital of Qiqihar Medical College |
Hanbing S.,The Third Affiliated Hospital of Qiqihar Medical College |
Hongxia B.,The Third Affiliated Hospital of Qiqihar Medical College |
Zhimin O.,Zhejiang University of Technology
Korean Journal of Chemical Engineering | Year: 2013
Tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate was synthesized using asymmetric reduction of tert-butyl (S)-6-chloro-5-hydroxy-3-oxo-hexanoate with liquid-core immobilized Saccharomyces cerevisiae CGMCC No. 2233. The optimum conditions for preparation of the liquid-core immobilized cells were found to be 2% guar gum, 5% CaCl2, 0. 8% sodium alginate, capsule diameter 2mm, 0. 3% chitosan (1. 0×105) solution, and 30 min for formation of the film of liquid-core immobilized cells. The optimum re-cultivation time was 32 h. The optimum reduction conditions were found to be pH 6. 8-7. 2, 160 r/min, and 30 °C. Conversion was found to reach 100% when initial concentration of substrate was less than 50 g/L. The diastereomeric excess of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate exceeded 99%. The liquid-core immobilized cells retained their effectiveness even after 15 uses. © 2012 Korean Institute of Chemical Engineers, Seoul, Korea.
Sun J.,Harbin Medical University |
Sun J.,Mudanjiang Medical College |
He W.,Harbin Medical University |
Bai S.-Z.,Harbin Medical University |
And 15 more authors.
Differentiation | Year: 2013
The calcium-sensing receptor (CaSR), a G protein coupled receptor, is involved in a number of physiological and pathological processes. Embryonic stem cells (ESCs) have a potential role to differentiate into all types of cells. Whether CaSR is functionally expressed in ESCs is unclear. In this study, the expression and distribution of CaSR in 129 mouse ES-D3 cell lines were detected by Western blotting and immunofluorescence; and the intracellular calcium concentration ([Ca2+]i) was measured using Laser Confocal Scanning Microscopy. Mouse embryonic stem cells (mESCs) were cultured to embryoid bodies (EBs) and the differentiation of EBs into cardiomyocytes was induced by icariin (ICA). The cardiac specific proteins, a-Actinin and cardiac troponin-I (cTnI), were analyzed by immunofluorescence, and the differentiation rate was analyzed by flow cytometry. The expression of cardiac-specific transcription factors, Nkx2.5 and GATA-4, was detected by Western blotting. We found that the CaSR protein exists in both mESCs and mESC-derived cardiomyocytes (mESC-CMs). Increasing extracellular calcium or neomycin (an agonist of CaSR) increased [Ca2+]i and the differentiation rate. These effects were abolished by inhibition of CaSR, phospholipase C, IP3 receptor and Ca2+ ATPase, or by depletion of the sarcoplasmic reticulum Ca2+ store, respectively. Activation of CaSR up-regulated protein expression of Nkx2.5 and GATA4 in EBs at an early stage of ICA-induced differentiation. In conclusion, CaSR is functionally expressed in mESCs, and activation of CaSR is involved in the differentiation of mESCs into cardiomyocytes by facilitating the expression of NKx2.5 and GATA-4. © 2012 International Society of Differentiation.
Liu H.-D.,The Third Affiliated Hospital of Qiqihar Medical College |
Tong J.-Z.,Harbin Medical University |
Qu B.,Harbin Medical University |
Jin S.-Z.,Harbin Medical University |
And 4 more authors.
World Chinese Journal of Digestology | Year: 2014
AIM: To investigate the correlation between functional dyspepsia (FD) and Helicobacter pylori (H. pylori) infection. METHODS: A total of 1570 FD patients with H. pylori infection were recruited. Four hundred FD patients with H. pylori infection who did not have abnormal social psychological factors were divided randomly into either a treatment group or a control group. The treatment group was further divided into an eradication success group or an eradication failure group by H. pylori eradication status one month after the eradication therapy. Twelve months after treatment, improvement of symptoms and the average cost of rescue medication were analyzed and compared between groups. RESULTS: In the H. pylori eradication success group, the symptoms improved more significantly compared with the H. pylori eradication failure group and control group one month after treatment, and the proportion of patients with symptomatic improvement ≥ 50% in the H. pylori eradication success group was significantly higher than those in the other two groups 12 mo after treatment. The average cost of rescue medication in the H. pylori eradication success group was significantly less than that in the control group and the H. pylori eradication failure group one month before the end of follow-up. CONCLUSION: H. pylori has a certain relevance to FD. H. pylori eradication in H. pylori positive FD patients who are not associated with abnormal social psychological factors can achieve good treatment effects. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved.