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Man S.,Tianjin University | Gao W.,Tianjin University | Zhang Y.,Tianjin University of Traditional Chinese Medicine | Huang L.,Chinese Institute of Materia Medica | Liu C.,The State Key Laboratories of Pharmacodynamics and Pharmacokinetics
Biomedical Chromatography

On-line ultra-performance liquid chromatography (UPLC) coupled with diode-array detection (UPLC/DAD) and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) were used for separation, identification and structural analyses of saponins in Rhizoma Paridis saponins (RPS) and rat plasma after oral administration of RPS. Thirty steroidal saponins in RPS were identified by comparing their retention time, accurate mass measurement and positive and negative mass spectrometry data with that of reference compounds. The UPLC/Q-TOF-MS method was proved to be rapid and efficient in that 30 steroidal saponins, including three kinds of saponins (prototype, pennogenyl and diosgenyl saponins) were tentatively characterized within 6min. After oral administration of RPS, 21 original saponins were absorbed in RPS-treated rat plasma. Our results indicated that UPLC/Q-TOF-MS is a rapid and effective tool for identification of a series of saponins at trace level. © 2010 John Wiley & Sons, Ltd. Source

Jiang Q.,Tianjin University | Gao W.,Tianjin University | Li X.,Tianjin University | Xia Y.,Tianjin University | And 5 more authors.
Food Hydrocolloids

The physicochemical, morphological and crystal structure characterization of the starches separated from rhizomes of Dioscorea opposita Thunb. Dioscorea alata Linn., Dioscorea nipponica Makino, Dioscorea bulbifera Linn. and Dioscorea septemloba Thunb. were studied and compared. Amylose content varied between 13.58% and 20.05%. Water-binding capacity, swelling power, solubility and total starch content of starches differed significantly. Scanning electron micrographs revealed that the surface was smooth or rough, the granules were oval to spherical and the size was obviously different. D. nipponica displayed A-type pattern. D. opposita D. alata, D. septemloba and D. bulbifera starches all exhibited C-type crystal. While the crystallinity degree of the starches separated from the five species were about 33.90%, 37.63%, 43.11%, 32.06% and 53.35%, respectively. The gelatinization transition temperatures (T o, T p and T c) and enthalpy of gelatinization (ΔH gel) and peak height index (PHI) were determined. D. OT, D. AL and D. BL starches showed the higher enthalpy of gelatinization. Pasting viscosity of D. OT and D. BL starches were much higher than others. The five plants could be separated into two groups: D. OT, D. AL and D. BL; D. ST and D. NM. © 2012 Elsevier Ltd. Source

Qu Z.,Tianjin University | Yang H.,Tianjin University | Zhang J.,Tianjin University | Huo L.,Tianjin University | And 4 more authors.
Neurochemical Research

Cerebralcare granule® (CG) is a preparation of Traditional Chinese Medicine that widely used in China. It was approved by the China State Food and Drug Administration for treatment of headache and dizziness associated with cerebrovascular diseases. In the present study, we aimed to investigate whether CG had protective effect against d-galactose (gal)-induced memory impairment and to explore the mechanism of its action. d-gal was administered (100 mg/kg, subcutaneously) once daily for 8 weeks to induced memory deficit and neurotoxicity in the brain of aging mouse and CG (7.5, 15, and 30 g/kg) were simultaneously administered orally. The present study demonstrates that CG can alleviate aging in the mouse brain induced by d-gal through improving behavioral performance and reducing brain cell damage in the hippocampus. CG prevents aging mainly via suppression of oxidative stress response, such as decreasing NO and MDA levels, renewing activities of SOD, CAT, and GPx, as well as decreasing AChE activity in the brain of d-gal-treated mice. In addition, CG prevents aging through inhibiting NF-κB-mediated inflammatory response and caspase-3-medicated neurodegeneration in the brain of d-gal treated mice. Taken together, these data clearly demonstrates that subcutaneous injection of d-gal produced memory deficit, meanwhile CG can protect neuron from d-gal insults and improve memory ability. © 2016 Springer Science+Business Media New York Source

Jiang Q.,Tianjin University | Gao W.,Tianjin University | Li X.,Tianjin University | Man S.,Tianjin University of Science and Technology | And 4 more authors.
Food Hydrocolloids

The effect of alkali-treatment for 0, 15, and 30 days at 35°C on amylose content, morphological properties, crystalline properties, swelling power, solubility, absorbance spectra and invitro digestibility of starches from Dioscorea zingiberensis, Dioscorea persimilis and Dioscorea opposita were investigated. The amylose content of all the three starches decreased after 15 days of alkaline treatment and then increased after 30 days of alkaline treatment. There were similar changes in relative crystallinity for D.zingiberensis and D.persimilis starches. It was observed that the three starches displayed a reduction trend in swelling power with a significant increase in solubility. Adhesion among some of the starch granules was observed after alkali-treatment for 30 days in D.zingiberensis and D.opposita starches, while D.persimilis starch showed some hollows on the granule surface. The rise in the absorbance ratio of 1047/1035 and 1047/1022cm-1 from FT-IR was observed during alkali-treatment for D.persimilis and D.opposita starches. Alkali-treatment elevated the invitro digestibility with resistant starch values climbing up from 50.16% to 64.95% and from 66.14% to 70.74% for D.zingiberensis and D.persimilis starches, respectively, but there was no significant change in resistant starch value for D. opposita starch. © 2014 Elsevier Ltd. Source

Zhang J.,College of Logistics | Zhang J.,Tianjin University | Gao W.,Tianjin University | Liu Z.,Tianjin University | And 2 more authors.
Iranian Journal of Pharmaceutical Research

High performance liquid chromatography (HPLC) with diode array detection (DAD) and electrospray ionization tandem mass spectrometry (ESI/MS/MS) was used to analyze the main components in the methanol extract of Fructus Aurantii (FA) and the metabolites in rat biological samples after oral administration of the methanol extract of FA. There were 31 constituents identified in the extract of FA including 2 alkaloids, 1 coumarin, 10 flavonoid glycosides and 18 ploymethoxylated favones. According to the UV spectrum and MS fragment character of main components in the methanol extract of FA, 18 parent constituents and 11 metabolites were tentatively identified in rat biological samples. Three groups of components in biological samples detected included flavonoid glycosides, their glucuronides and ploymethoxylated favones. It was interested that flavonoid glycosides, their glucuronides and ploymethoxylated favones can be investigated in rat plasma and urine, while in rat feces samples only flavonoid glycosides were detected. Triglycosyl, naringenin, neoeriocitrin, neoeriocitrin narirutin and hesperidin were the main components in rat feces which were found either in the plasma or in urine samples. However, naringin and neohesperidin were the main flavonoid glycosides which absorbed after oral administration. Except flavonoid glycosides and their glucuronides, ploymethoxylated favones also the constituents absorbed because it was investigated mainly in rat plasma and urine but not in feces samples. The identification and elucidation of parent and metabolism components analyzed in biological samples provided the data for further pharmacological and clinical research on FA. © 2014 by School of Pharmacy. Source

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