The Sixth Peoples Hospital of Hangzhou

Hangzhou, China

The Sixth Peoples Hospital of Hangzhou

Hangzhou, China
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Zhang Y.-L.,The Sixth Peoples Hospital of Hangzhou
Chinese Journal of Microbiology and Immunology (China) | Year: 2011

Objective: To establish a rapid, sensitive, and specific quantitative method to detect hepatitis C virus. Methods: A primer set targeting HCV 5'UTR was designed. The isothermal amplification was performed by the Bst DNA polymerase and AMV reverse transcriptase, under the temperature of 60°C for 60 min. The signal was monitored by SYBR Green I. Results: One hundred and twenty positive serum samples, confirmed by the real-time PCR. All were detected by the isothermal amplification, while 110 healthy subjects' samples were negative by the both methods. The lower detect limit was determined to 10 IU/ml HCV-RNA, by the assay on serial dilutions of the quality control standards obtained from clinical investigation center of MOH. Conclusion: A real time reverse loop-mediated isothermal amplification method was developed to detect HCV, with the characteristic of rapidity, high sensitivity and specificity. Copyright © 2011 by the Chinese Medical Association.


Zhang F.,Ningbo Development Zone Center Hospital | Huang W.,The Sixth Peoples Hospital Of Hangzhou | Sheng M.,Hospital of Longsai | Liu T.,Shanghai University
Tumor Biology | Year: 2015

Recent studies have shown that microRNA-451 (miR-451) was significantly decreased in osteosarcoma tissues and was identified as a tumor suppressor in other types of human cancers. However, its clinical significance and molecular mechanisms in osteosarcoma are still not well understood. MiR-451 levels are evaluated by quantitative reverse transcription-polymerase chain reaction (RT-PCR) in osteosarcoma cell lines and in 68 pairs of osteosarcoma and adjacent noncancerous tissues. Then, the associations of miR-451 expression with clinicopathological features of patients were determined. The effects of miR-451 in osteosarcoma cells were examined by MTT and Matrigel invasion assay. The functional target of miR-451 were determined by bioinformatics analysis and validated by luciferase reporter analyses and Western blot assay. Our results showed that the expression of miR-451 was significantly downregulated in osteosarcoma tissues compared with corresponding noncancerous tissues (P < 0.01). Particularly, statistical analysis of primary human osteosarcoma indicated that decreased expression of miR-451 was correlated with metastasis and recurrence. Moreover, the miR-451 force-expression suppressed cell proliferation and invasion in vitro. Based on bioinformatics analysis, we found that chemokine ligand 16 (CXCL16) was identified as a direct functional target of miR-451. Consistent with the effects of miR-451, silencing CXCL16 could phenocopy the effects of miR-451 on phenotypes of osteosarcoma cells. Furthermore, CXCL16 expression was upregulated in osteosarcoma tissues and inversely associated with miR-451 in human osteosarcoma tissues. Our data reveal a downregulated expression of miR-451 in osteosarcoma tissues, which is inversely associated with CXCL16 levels. These observations demonstrated that miR-451 may play an important role in tumor growth and metastasis in osteosarcoma. © 2014, International Society of Oncology and BioMarkers (ISOBM).


Guo L.-K.,The First Affiliated Hospital of Xinxiang Medical College | Zhang C.-X.,The First Affiliated Hospital of Xinxiang Medical College | Guo X.-F.,The Sixth Peoples Hospital of Hangzhou
Journal of Chinese Integrative Medicine | Year: 2011

Background: Functional constipation is one of the most common gastrointestinal diseases. Currently, there is no effective Western medical therapy for functional constipation and it significantly impacts the quality of life of the patients. Integrated traditional Chinese and Western medicine therapies were reported to have better therapeutic effects than routine Western medicine therapies. Objective: To explore the efficacy of acupuncture combined with Chinese herbal medicine Plantain and Senna Granule in the treatment of functional constipation. Design, setting, participants and interventions: A total of 390 patients with confirmed functional constipation enrolled from the Department of Gastroenterology, the First Affiliated Hospital of Xinxiang Medical College in China from February 2008 to January 2010 were randomly divided into three groups, with 130 cases in each group. Acupuncture group was treated with puncture of point Tianshu (ST25), Shangjuxu (ST37), Zusanli (ST36), Dachangyu (BL25) and Zhigou (SJ6) twice daily for four weeks, while Plantain and Senna Granule group was treated with 5 g of Plantain and Senna Granule once daily, and the combination group was given above-mentioned acupuncture and Plantain and Senna Granule. Main outcome measures: The defecation cycle, stool property, constipation symptom, accompanying symptoms, gastrointestinal transit time, including total gastrointestinal transit time, mouth-intestine transit time, colonic transit time, right colonic transit time, left colonic transit time and rectosigmoid colonic transit time, and adverse reactions of the three groups were evaluated before treatment, at the end of treatment and 64 weeks after treatment, respectively. Results: Compared with before treatment, the scores of defecation cycle, stool property, constipation symptoms and accompanying symptoms, and gastrointestinal transit time all decreased significantly at the end of treatment in each group (P<0. 01), and the combination group showed better results than the other groups (P<0.05). Compared with the end of treatment, the above-mentioned indexes recurred insignificantly in the combination group and acupuncture group after 64 weeks of follow-up (P>0. 05), but all recurred significantly in the Plantain and Senna Granule group (P>0. 05). Both short- and long-term total response rates of the combination group showed significant differences compared with those of the other two groups (P<0. 05 or P<0.01). No serious adverse reactions occurred in all the three groups. Conclusion; Acupuncture plus Plantain and Senna Granule can significantly decrease gastrointestinal transit time and defecation cycle in patients with functional constipation; it can also change stool property and alleviate constipation symptom and accompanying symptoms, with good security and tolerance.


Zhang C.,Xinxiang Medical University | Guo L.,Xinxiang Medical University | Guo X.,Xinxiang Medical University | Guo X.,The Sixth Peoples Hospital of Hangzhou | Li G.,Xinxiang Medical University
Journal of Traditional Chinese Medicine | Year: 2012

OBJECTIVE: To study the clinical curative effect, safety and mechanism of action of electroacupuncture combined with Zhizhukuanzhong capsules (ZZKZC) in treating gastroesophageal reflux disease (GERD). METHODS: A total of 480 patients with confirmed GERD were randomly divided into four groups: the electroacupuncture group, the ZZKZC group, the combined therapy group, and the control group, with 120 cases in each group. Each case in the electroacupuncture group was treated with electroacupuncture on Zusanli (ST 36), Zhongwan (CV 12), Neiguan (PC 6), Taichong (LR 3) and Gongsun (SP 4) once daily for 6 weeks. Each case in the ZZKZC group was treated with oral administration of 1.29 g ZZKZC three times daily. The combined therapy group had electroacupuncture and ZZKZC. The control group was given oral administration of 5 mg mosapride three times and 20 mg pantoprazole twice daily. The 24-hour intraesophageal total number of reflux episodes with pH <4 (or bilirubin absorbance ≥ 0.14), the number of long-term (≥5 min) reflux episodes, the percentage of upright time, the percentage of supine time, the percentage of total time of pH <4 (or bilirubin absorbance ≥0.14), endoscopic grading score, symptom score, quality of life score, and adverse reactions were observed before treatment, at the end of treatment and 54 weeks after treatment in the four groups. RESULTS: The 24-hour intraesophageal pH and bile reflux, endoscopic grading score and symptom score were all significantly decreased at the end of treatment in every group, while the scores of 8 dimensions of quality of life were all increased compared with those before treatment (P<0.01). All of these indices were better in the combined therapy group than those in the other groups (P<0.05). These indices did not significantly deteriorate in the combined therapy group and electroacupuncture group 54 weeks after treatment compared with the end of treatment (P>0.05); however, these indices all significantly deteriorated in the ZZKZC and control groups (P>0.05). The short and long-term total efficacy rates in the combined therapy group showed significant superiority to those in the other groups (P<0.05 or P<0.01). No serious adverse reactions were found in the four groups. CONCLUSION: Electroacupuncture and ZZKZC play an important role in inhibiting intraesophageal acid and bile reflux, decreasing the endoscopic grading score, and alleviating the symptoms of gastroesophageal reflux to improve the quality of life. However, the effect of combined treatment is more effective, with better security and long-term efficacy, and therefore, this combination treatment is appropriate for clinical use. © 2012 JTCM. All rights reserved.


Zhang C.-X.,Xinxiang Medical University | Guo L.-K.,Xinxiang Medical University | Guo X.-F.,The Sixth Peoples Hospital of Hangzhou
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2015

Objective: To investigate the correlation of cigarette smoking and the combination of polymorphisms of β3-adrenergic receptor (β3-AR) gene Trp64Arg and manganese superoxide dismutase9Ala/Val (MnSOD9Ala/Val) genes with nonalcoholic fatty liver disease (NAFLD). Methods: The genetic polymorphisms of β3-AR gene Trp64Arg and MnSOD9Ala/Val were analyzed by polymorphism-polymerase chain reaction (PCR) technique in peripheral blood leukocytes of 720 NAFLD patients and 720 healthy persons. Results: The frequency of β3-AR gene Trp64Arg (A/A) and MnSOD9Ala/Val (V/V) was 39.4% and 71.7% in NAFLD patients and 21.1% and 43.3% in healthy controls, respectively. Statistical tests showed significant differences in the frequency between the two groups (P<0.01). The risk of NAFLD in patients carrying Trp64Arg(A/A) was significantly higher than that in the controls (OR=2.434, 95% CI=1.816-4.075). The individuals carrying MnSOD9Ala/Val (V/V) had a higher risk of NAFLD (OR=3.308, 95% CI=1.913-4.509). Combined analysis of the polymorphisms showed that the percentage of Trp64Arg (A/A)/MnSOD9Ala/Val (V/V) in NAFLD and control groups was 32.8% and 6.5%, respectively (P<0.01). The patients carrying Trp64Arg (A/A)/MnSOD9Ala/Val (V/V) had a higher risk of NAFLD (OR=9.753, 95% CI=4.292-12.426). The smoking rate of the case group was significantly higher than that in the control group (OR=2.623, 95% CI=1.425-4.957, P<0.01), and statistic analysis suggested an interaction between cigarette smoking and Trp64Arg(A/A)/MnSOD9Ala/Val(V/V) which increased the risk of NAFLD (OR=33.764, 95% CI=18.907-61.582). Conclusion: Trp64Arg(A/A), MnSOD9Ala/Val (V/V) and cigarette smoking are the risk factors for NAFLD, and they can play a synergetic role in NAFLD. ©, 2015, Xi'an Medical University. All right reserved.


Zhang C.-X.,Xinxiang Medical University | Guo L.-K.,Xinxiang Medical University | Guo X.-F.,The Sixth Peoples Hospital of Hangzhou
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2015

Objective: To explore the expressions of Toll-like receptor4/NF-κB and PI3K/AKT/NF-κB signaling pathways in rat ulcerative colitis (UC) induced by the combined enema of trinitrobenzene sulphonic acid and ethano and the interventional effect of electroacupuncture on them. Methods: Totally 240 male Wistar rats were randomly divided into 6 groups: normal control group, model control group, electroacupuncture group, TLR4mAb group, LY294002 group, and TLR4mAb combined with LY294002 (T&L) group. The combined enema of trinitrobenzene sulphonic acid (TNB) and ethanol was intrarectally administered for 4 weeks to induce UC. At the same time of modeling, Zusanli point was electro-acupunctured in electroacupuncture group while intraperitoneal injection of TLR4mAb and LY294002 was given respectively to the corresponding group. Each rat was treated with the above-mentioned TLR4mAb injection and LY294002 injection in T&L group for 4 weeks. The disease activity index (DAI) of all the rats was evaluated daily. The rats were killed after 4 weeks. The colonic mucosa damage index (CMDI) and tissue damage index (TDI) were evaluated by a pathologic grading system. The expressions of P-Akt and active NF-κB protein in the colon mucosa were determined by Western blotting. TLR4 mRNA, PI3K mRNA, AKT mRNA, NF-κB mRNA, TNF-αmRNA and IL-1βmRNA expressions were measured with RT-PCR. Results: Compared with those in normal control group, TLR4 mRNA, PI3K mRNA, P-AKT, active NF-κB, TNF-α mRNA and IL-1β mRNA expressions as well as DAI, CMDI and TDI were all increased obviously in model control group (P<0.01). Compared with those in model control group, TLR4mRNA expression was decreased obviously in TLR4mRNA group (P<0.01), the expressions of PI3KmRNA and P-AKT were decreased obviously in LY294002 group (P<0.01). Not only TLR4mRNA expression but also PI3KmRNA and P-AKT expressions were decreased significantly in electroacupuncture group and T&L group (P<0.01). Corresponding to the above-mentioned changes, active NF-κB, TNF-αmRNA and IL-1βmRNA expressions as well as DAI,CMDI and TDI were decreased obviously in all the treated groups compared with those in model control group (P<0.05 or P<0.01), but the six indexes were better in electroacupuncture group and T&L group than in TLR4mAb group and LY294002 group (P<0.05). There were obvious positive correlations of active NF-κB with TNF-αmRNA and IL-1β mRNA expressions (r1=0.579, P<0.05; r2=0.561, P<0.05). Conclusion: Electroacupuncture can significantly decrease NF-κB activity and TNF-αmRNA and IL-1β mRNA expressions in UC rats, thus alleviating the severity of UC, which is closely correlated to its blocking both TLR4/NF-κB and PI3K/AKT/NF-κB signaling pathways. ©, 2015, Xi'an Medical University. All right reserved.


PubMed | the Sixth peoples Hospital of Hangzhou and Xinxiang Medical University
Type: Journal Article | Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae | Year: 2015

To investigate the interaction of the polymorphisms of cyclooxygenase-2-1195G/A (COX-2-1195G/A) and manganese superoxide dismutase 9Ala/Val (MnSOD9Ala/Val) genes and the high-fat diets and its potential correlation with ulcerative colitis (UC).The genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val were analyzed by polymorphism-polymerase chain reaction (PCR) in peripheral blood leukocytes obtained from 750 UC patients (UC group) and 750 healthy subjects (control group).The frequencies of COX-2-1195G/A(A/A) and MnSOD9Ala/Val(V/V) were 49.07% and 50.13% in UC group and 21.20% and 22.40% in control group, respectively (P<0.01). The risk of UC significantly increased in subjects with COX-2-1195G/A(A/A) genotype (OR=3.5808,95%CI=1.8062-5.3478) and in those with MnSOD9Ala/Val(V/V) genotype(OR=3.4828,95%CI=1.9137-5.5496). Pooled analysis of the polymorphisms showed that distribution frequency of COX-2-1195G/A(A/A)/MnSOD9Ala/Val (V/V) was 40.67% in UC group and 8.40% in control group (P<0.01). Subjects with COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V) had a significantly higher risk of UC (OR=7.5655,95% CI=4.1849-11.2037). The rate of high-fat diets was significantly higher in the UC group than in the control group(49.73 vs.20.13%,P<0.01),and statistic analysis suggested an interaction between high-fat diet and COX-2-1195G/A(A/A)(=11.81821)and MnSOD9Ala/Val (V/V)(=9.0107), which increase risk of UC.COX-2-1195G/A(A/A),MnSOD9Ala/Val (V/V), and high-fat diet are the risk factors of UC. The interaction between the genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val and the high-fat diet increases the risk of UC.


Shi Q.,Soochow University of China | Gao W.,the Sixth Peoples Hospital of Hangzhou | Han X.L.,Soochow University of China | Zhu X.S.,Soochow University of China | And 7 more authors.
Science China Life Sciences | Year: 2014

Nerve conduit is one of strategies for spine cord injury (SCI) treatment. Recently, studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site. However, the scaffold by itself was difficult to meet the need of SCI functional recovery. The basic fibroblast growth factor (bFGF) administration significantly promotes functional recovery after organ injuries. Here, using a rat model of T9 hemisected SCI, we aimed at assessing the repair capacity of implantation of collagen scaffold (CS) modified by collagen binding bFGF (CBD-bFGF). The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI. The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group, when evaluated by the 21-point Basso-Beattie-Bresnahan (BBB) score and footprint analysis. Both hematoxylin and eosin (H&E) and immunohistochemical staining of neurofilament (NF) and glial fibrillary acidic protein (GFAP) demonstrated that fibers were guided to grow through the implants. These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery. © 2014 The Author(s).


Bao J.F.,The Sixth Peoples Hospital of Hangzhou
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2011

To investigate the dynamic expression of TGF-beta1/Smad protein in rats with liver fibrosis induced by carbon tetrachloride (CCl4), to study mechanism of TGF-beta1/Smad signaling and the relationship between its transduction and liver fibrosis. Fifty healthy male SD rats were randomly divided into two groups: normal control group and model group. Experimental liver fibrosis was induced by subcutaneous injection of CCl4. After six weeks and nine weeks, histopathological changes and degrees of fibrosis were observed by optical microscopy. Meanwhile, the expression of TGF-beta1, TP3R-I, Smad2/3 and Smad7 proteins was detected by immunohistochemistry. (1) Pathological observation of hepatic specimens: hepatic tissue of model group rat had inflammation and fibrosis in different degrees. By comparing with the degrees of inflammation and fibrosis model groups were more severe than normal control group (P < 0.05). (2) Changes of protein levels about TGF-beta1/Smad: the expressions of TGF-beta1, TbetaR-I, Smad2/3 and Smad7 in rat hepatic tissue were detected with immunohistochemistry techniques. The expressions of the four items in model group were higher than those of normal control group (P < 0.01). In fibrosis model group, there exist considerable positive correlations among expressions of TGF-beta1, TbetaR-I, Smad2/3, Smad7 and degrees of fibrosis in livers (P < 0.05 or 0.01). There is close relation between the level of TGF-beta1, TbetaR-I, Smad2/3, Smad7 and the different liver fibrosis grades due to CCl4. The up regulation of TGF-beta1, TbetaR- I, Smad2/3 and Smad7 in liver tissue is involved in the progression of hepatic fibrosis.


Xun Y.H.,The Sixth Peoples Hospital of Hangzhou
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010

To observe the Chinese medicine constitution types and human leukocyte antigen (HLA)-DQA1 gene polymorphism in patients with hepatitis B (HB) virus infection in Chinese Han population of Zhejiang Province, for exploring the roles of constitution factor in pathogenesis of HB. A total of 240 subjects, including 120 biopsy-proven chronic HB (CHB), 60 HB asymptomatic carrier (ASC) and 60 resolved from HBV infection spontaneously (RHBS) were studied. Their Chinese medicine constitution type was judged by Wangqi's classification, and their genotype of HLA-DQA1 was detected by polymerase chain reaction sequence specific primer for comparing the difference between groups in distribution frequency (DF) of constitution types and genes. (1) As compared with the RHBS group, DF of yin-deficiency constitution and phlegm-dampness constitution in the CHB group was significant higher (20.0% vs. 6.7% and 12.5% vs. 1.7%), and that of placid constitution was significant lower (11.7% vs. 31.7%), showing statistical significance between groups (OR = 3.5, 95% CI: 1.16-10.60; OR = 8.4, 95% CI: 1.09-65.42; OR = 0.161, 95% CI: 0.076-0.34; all P < 0.05). (2) As compared with the ASC group, DF of damp-heat constitution was significant higher (24.2% vs. 6.7%, P < 0.05, OR = 4.462, 95% CI: 1.49-13.36), and that of placid constitution was significant lower (11.7% vs. 45.0%, P < 0.01, OR = 0.285, 95% CI: 0.13-0.62) in the CHB group. (3) As compared with RHBS group, DF of HLA-DQA1 * 0201 allele in CHB group was significant higher (38.3% vs. 5.8%, P < 0.01, OR = 10.04, 95% CI: 4.48-22.48); and that of HLA-DQA1 * 0102 allele was significant lower (9.6% vs. 36.7%, P < 0.01, OR = 0.183, 95% CI: 0.10-0.32). (4) As compared with ASC group, DF of HLA-DQA1 * 0201 allele in CHB group was significant higher (38.3% vs. 7.5%, P < 0.01, OR = 7.667, 95% CI: 3.7-15.87), and that of HLA-DQA1 * 0102 allele was significant lower (20.0% vs. 9.6%, P < 0.01, OR = 0.424, 95% CI: 0.23-0.79). Both Chinese medicine constitution and HLA-DQA1 gene polymorphism show connection with the outcomes of HB virus infection in Chinese Han population, but the real association between them is required for further study.

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