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Bao J.F.,The Sixth Peoples Hospital of Hangzhou
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2011

To investigate the dynamic expression of TGF-beta1/Smad protein in rats with liver fibrosis induced by carbon tetrachloride (CCl4), to study mechanism of TGF-beta1/Smad signaling and the relationship between its transduction and liver fibrosis. Fifty healthy male SD rats were randomly divided into two groups: normal control group and model group. Experimental liver fibrosis was induced by subcutaneous injection of CCl4. After six weeks and nine weeks, histopathological changes and degrees of fibrosis were observed by optical microscopy. Meanwhile, the expression of TGF-beta1, TP3R-I, Smad2/3 and Smad7 proteins was detected by immunohistochemistry. (1) Pathological observation of hepatic specimens: hepatic tissue of model group rat had inflammation and fibrosis in different degrees. By comparing with the degrees of inflammation and fibrosis model groups were more severe than normal control group (P < 0.05). (2) Changes of protein levels about TGF-beta1/Smad: the expressions of TGF-beta1, TbetaR-I, Smad2/3 and Smad7 in rat hepatic tissue were detected with immunohistochemistry techniques. The expressions of the four items in model group were higher than those of normal control group (P < 0.01). In fibrosis model group, there exist considerable positive correlations among expressions of TGF-beta1, TbetaR-I, Smad2/3, Smad7 and degrees of fibrosis in livers (P < 0.05 or 0.01). There is close relation between the level of TGF-beta1, TbetaR-I, Smad2/3, Smad7 and the different liver fibrosis grades due to CCl4. The up regulation of TGF-beta1, TbetaR- I, Smad2/3 and Smad7 in liver tissue is involved in the progression of hepatic fibrosis. Source


Zhu H.,U.S. Center for Disease Control and Prevention | Ding X.,Tianyuan Bio-Pharmaceutical | Chen X.,Tianyuan Bio-Pharmaceutical | Yao P.,U.S. Center for Disease Control and Prevention | And 12 more authors.
Vaccine | Year: 2011

In order to develop an animal model and an assay method to evaluate protective immune response to H5N1 avian influenza vaccination, H5N1 avian influenza vaccine was prepared. New Zealand rabbits were assigned to receive two doses of vaccine with different hemagglutinin (HA) dosage. The sera from vaccinated rabbits was evaluated to determine antibody titer and specificity using different tested methods including hemagglutination inhibition assay (HI), neutralizing assay (NT), cross-HI assay, cross-single immunodiffusion assay and cross-neutralization assay. The titer of HI antibody from rabbits immunized with different doses of HA were no less than 1:40 among groups 14 days after the first immunization. Whereas the NT antibody titer was less than 1:10 among groups 14 days after the first immunization. NT antibodies can be detected 14 days after the second immunization in rabbits immunized at HA doses higher than 6 μg, and the NT antibody titers were equal to or higher than 1:40. A good concentration-dependent NT antibody response can be detected in the vaccinated rabbits 14 days after the second immunization, and in contrast, no concentration-dependent relationship can be seen for HA antibody. The cross-HI test showed sera from vaccinated rabbits could cross react with influenza A H5N1 virus with the titers higher than 1:40. No cross reaction among different types (influenza A/H1N1 virus, influenza A/H3N2 virus, influenza B virus and influenza A/H5N1 virus) can be detected in the sera using the single immunodiffusion assay and using NT antibody test. This showed NT antibody test was demonstrated as a more accurate assay method for evaluating vaccination and quality of the vaccine than HI antibody test. © 2011 Elsevier Ltd. Source


Li H.,Zhejiang UniversityZhejiang | Huang D.,Chongqing Cancer Institute | Jiang L.,The Sixth Peoples Hospital of Hangzhou | Yao J.,Hang Zhou Plastic Surgery Hospital | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

This study was designed to investigate risk factors for lymph node metastasis of the Chinese people with early stage colorectal cancer, which was confirmed to a carcinoma that invaded the submucosa after radical resection. In total, 68 patients revealing submucosal invasive colorectal carcinoma on pathology who underwent curative radical resection from October 2007 to September 2011 were evaluated retrospectively. Tumor size, depth of submucosal invasion, histological grade, lymph-vascular invasion, tumor budding, and microacinar structure were reviewed independently by two pathologists. Student t-test for continuous variables and Chi-square test for categorical variables were used for comparing the clinic-pathological features between two groups (whether lymph node involvement existed or not). Continuous variables are expressed as the mean ± standard error while statistical significance is accepted at P < 0.05. As results, the mean age of the patients was 65.7 ± 9.6 years (range from 47 to 84). The mean tumor size (the largest diameter) was 21.5 ± 1.2 mm (range from 5 to 84 mm). Histologically, 11 patients (16.2%) had metastatic lymph node. The lymph node metastasis was significantly associated with the tumor budding (P = 0.038) and the tumor size (P = 0.031) while other factors were not statistically significant. Tumor budding seems to have a high sensitivity (80.0%) for lymph node metastasis, acceptable specificity (55.6%). Therefore, tumor budding should be performed in order to clarify early colorectal cancer with lymph node metastasis through pathologic analysis. © 2016, E-Century Publishing Corporation. All rights reserved. Source


Zhang C.-X.,The First Affiliated Hospital of Xinxiang Medical College | Guo L.-K.,The First Affiliated Hospital of Xinxiang Medical College | Guo X.-F.,The Sixth Peoples Hospital of Hangzhou
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2012

Objective: To observe the effects of electroacupuncture combined with Fuzheng-Liqi mixture on growth and metastasis of rat liver cancer and to explore the related mechanisms. Methods: Totally 200 male Wistar rats were randomly divided into five groups. Liver cancer model was induced with intragastric administration of diethylnitrosamine (DEN). At the same time of modeling, each rat in electroacupuncture group was treated with electroacupuncture of points Zusanli, Guanyuan, Neiguan, Sanyinjiao and Ganyu while Fuzheng-Liqi mixture was poured into the gastric cavity in Fuzheng-Liqi mixture group, and each rat was treated with the above-mentioned puncture and Fuzheng-Liqi mixture. After 16 weeks the rats were killed to check tumor growth and metastasis with the naked eye and light microscope. NF-κB activity and microvascular density (MVD) in liver tissues were determined with immunohistochemical method; TGF-β1mRNA and VEGFmRNA expressions were measured with RT-PCR method. And the levels of ROS, T-AOC, SOD, GSH-Px, CAT and LPO in liver tissues were determined with radioimmunoassay. Results: Compared with those in normal control group, tumor growth and metastasis parameters, ROS, LPO, NF-κB activity, TGF-β1 mRNA, VEGF mRNA and MVD expressions were increased obviously while the levels of T-AOC, SOD, GSH-Px and CAT were decreased obviously in model group (P<0.01); Compared with those in model control group, tumor growth and metastasis parameters, ROS, LPO, NF-κB activity, TGF-β1 mRNA, VEGF mRNA and MVD expressions were decreased obviously while the levels of T-AOC, SOD, GSH-Px and CAT were increased obviously in all the treatment groups (P<0.01). The above-mentioned indexes were better in combination group than in other treatment groups (P<0.05). NF- B activity had an obvious positive correlation with TGF-β1 mRNA and VEGFmRNA expressions (r 1=0.554, P<0.05; r 2=0.572, P<0.05). Conclusion: Electroacupuncture and Fuzheng-Liqi mixture can obviously decrease NF-κB activity, TGF-β1 mRNA, VEGFmRNA and MVD expressions in rats with hepatic tumor, thus decreasing tumor growth and metastasis parameters. This is closely correlated to their scavenging effects on free radicals. Source


Shi Q.,Soochow University of China | Gao W.,The Sixth Peoples Hospital of Hangzhou | Han X.L.,Soochow University of China | Zhu X.S.,Soochow University of China | And 7 more authors.
Science China Life Sciences | Year: 2014

Nerve conduit is one of strategies for spine cord injury (SCI) treatment. Recently, studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site. However, the scaffold by itself was difficult to meet the need of SCI functional recovery. The basic fibroblast growth factor (bFGF) administration significantly promotes functional recovery after organ injuries. Here, using a rat model of T9 hemisected SCI, we aimed at assessing the repair capacity of implantation of collagen scaffold (CS) modified by collagen binding bFGF (CBD-bFGF). The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI. The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group, when evaluated by the 21-point Basso-Beattie-Bresnahan (BBB) score and footprint analysis. Both hematoxylin and eosin (H&E) and immunohistochemical staining of neurofilament (NF) and glial fibrillary acidic protein (GFAP) demonstrated that fibers were guided to grow through the implants. These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery. © 2014 The Author(s). Source

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