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Gan W.,University of Chinese Academy of Sciences | Wu J.,University of Chinese Academy of Sciences | Lu L.,University of Chinese Academy of Sciences | Xiao X.,The Sichuan Provincial Peoples Hospital | And 9 more authors.
PLoS ONE | Year: 2012

Dysregulation of the complement system has been linked to pathogenesis of hypertension. However, whether genetic changes of complement factor H (CFH) and its related genes are associated with hypertension is unknown. We genotyped three SNPs in the CFH gene cluster that are closely linked to age-related macular degeneration, namely rs1061170 (Y402H), rs2274700 (A473A) and rs7542235 (CFHR1-3Δ), and tested for their associations with blood pressure and hypertension risk in a population-based cohort including 3,210 unrelated Chinese Hans (50-70 years of age) from Beijing and Shanghai. We found that rs2274700 (A473A) and rs7542235 (CFHR1-3Δ) were both significantly associated with diastolic blood pressure (DBP) (β = 0.632-1.431, P≤0.038) and systolic blood pressure (SBP) (β = 1.567-4.445, P≤0.008), and rs2274700 (A473A) was associated with hypertension risk (OR [95%CI]: 1.175 [1.005-1.373], P = 0.048). Notably, the associations of rs2274700 (A473A) with DBP (P = 2.1×10-3), SBP (P = 8×10-5) and hypertension risk (P = 7.9×10-3) were significant only in the individuals with low CRP levels (<2.0 mg/l), but not in those with CRP levels ≥2.0 mg/l (P≥0.0807) (P for interaction ≤0.0467). However, no significant association between rs1061170 (Y402H) and blood pressure or hypertension risk was observed (P≥0.259). In conclusion, our results suggest that genetic variations in CFH and its related genes may contribute to hypertension risk in Chinese Hans. © 2012 Gan et al. Source

Zhang H.,Chengdu University of Traditional Chinese Medicine | Zhao L.,Chengdu University of Traditional Chinese Medicine | Yang S.,Chengdu University of Traditional Chinese Medicine | Chen Z.,Chengdu University of Traditional Chinese Medicine | And 4 more authors.
Journal of Traditional Chinese Medicine | Year: 2013

OBJECTIVE: To evaluate the therapeutic effect of scalp electroacupuncture for mild cognitive impairment (MCI) in the early stage. METHODS: Two hundred and thirty three MCI patients were randomly divided into three groups: the drug group, the scalp electroacupuncture group, and the syndrome differentiation group. For the scalp electroacupuncture group, the points of Baihui (DU 20), Sishecong (EX-HN1), Fengchi (GB 20), and Shenting (DU 24) were selected. For the syndrome differentiation group, specific acupoints were added on the basis of syndrome differentiation and according to the scale for the differentiation of syndromes in vascular dementia (SDSVD) beside the acupoints used in the scalp electroacupuncture group. For the drug group, nimodipine was orally administered. Each patient was treated for two courses, eight weeks. The score differences in mini-mental state examination (MMSE), picture recognition, and clock drawing test were observed before and after the treatment. RESULTS: After treatment, the score differences in MMSE and clock drawing test were of obvious statistical significance among three groups (P<0.01, P<0.05). The score differences in picture recognition were of extremely statistical significance between the scalp electroacupuncture group and the syndrome differentiation group (P<0.01), while the difference was not found in the drug group (P>0.05). There were statistical significant differences in therapeutic effects between the scalp electroacupuncture group and the drug group, and between the syndrome differentiation group and the drug group (P<0.05), while no statistical difference was found between scalp electroacupuncture group and the syndrome differentiation group (P>0.05). CONCLUSION: All the three therapies may improve the cognitive function of MCI patients. The therapeutic effects in the scalp electroacupuncture and syndrome differentiation groups were basically the same, but superior to nimodipine. © 2013 JTCM. All rights reserved. Source

Tang J.,The Sichuan Provincial Peoples Hospital | Wang Z.,The Sichuan Provincial Peoples Hospital | Chen L.,The Sichuan Provincial Peoples Hospital | Huang G.,The Sichuan Provincial Peoples Hospital | Hu X.,The Sichuan Provincial Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2015

It is reported that gossypol acetate (GAA) has obvious effects on inhibiting the growth of tumors, by inhibiting the activity of enzymes. Ultrastructural study showed that GAA can cause morphological changes of mitochondria which leads to the apoptosis of tumors. However, little is known about the pathways that how the GAA triggers apoptosis of tumors and what kind of the molecular events have happened when GAA is added. The aim of the study is try to know if GAA have some functions on pituitary tumor cell. And if there are any changes after GAA treatment, we try to understand the mechanisms that how GAA regulate the growth of pituitary tumor cell. The study was carried out on rat lactotroph cell lines, GH3 and MMQ. Q-PCR and western blot (WB) assay are used to determine the expression level of genes and the protein level. Both the miR-15a (mimics) overexpression cell line and miR-15a knock out (inhibitor) cell line were obtained in GH3 and MMQ. Apoptosis rate was determined by flow cytometry (FCM). Our study revealed that: 1) GAA inhibits the proliferation of pituitary tumor cells of GH3, MMQ. 2) GAA upregulate the expression of miR15a in GH3 and MMQ. 3) Overexpressed miR-15a (mimics) downregulates the expression level of Bcl-2. 4) Knock down miR-15a (inhibitor) upregulates Bcl-2 and reverse the apoptosis induced by GAA. Our study indicates that GAA-induced decrease in cell proliferation is associated with decreased expression of Bcl-2 and increased miR-15a. Based on this, we propose developing GAA as a novel therapeutic tool in the management of pituitary tumor. © 2015, E-Century Publishing Corporation. All rights reserved. Source

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