Li X.,The Second Peoples Hospital of Yunnan Province |
Lu P.,The Second Peoples Hospital of Yunnan Province |
Li B.,The Second Peoples Hospital of Yunnan Province |
Zhang W.,The Second Peoples Hospital of Yunnan Province |
And 3 more authors.
International Journal of Biochemistry and Cell Biology | Year: 2017
The precise role of interleukin (IL)-10 in breast cancer is not clear. Previous studies suggested a tumor-promoting role of IL-10 in breast cancer, whereas recent discoveries that IL-10 activated and expanded tumor-resident CD8+ T cells challenged the traditional view. Here, we investigated the role of IL-10 in HLA-A2-positive breast cancer patients with Grade III, Stage IIA or IIB in-situ and invasive ductal carcinoma, and compared it with that of IL-2, the canonical CD8+ T cell growth factor. We first observed that breast cancer patients presented higher serum levels of IL-2 and IL-10 than healthy controls. Upon prolonged TCR stimulation, peripheral blood CD8+ T cells from breast cancer patients tended to undergo apoptosis, which could be prevented by the addition of IL-2 and/or IL-10. The cytotoxicity of TCR-activated CD8+ T cells was also enhanced by exogenous IL-2 and/or IL-10. Interestingly, IL-2 and IL-10 demonstrated synergistic effects, since the enhancement in CD8+ T cell function when both cytokines were added was greater than the sum of the improvements mediated by each individual cytokine. IL-10 by itself could not promote the proliferation of CD8+ T cells but could significantly enhance IL-2-mediated promotion of CD8+ T cell proliferation. In addition, the cytotoxicity of tumor-infiltrating CD8+ T cells in breast tumor was elevated when both IL-2 and IL-10 were present but not when either one was absent. This synergistic effect was stopped by CD4+CD25+ regulatory T cells (Treg), which depleted IL-2 in a cell number-dependent manner. Together, these results demonstrated that IL-2 and IL-10 could work synergistically to improve the survival, proliferation, and cytotoxicity of activated CD8+ T cells, an effect suppressible by CD4+CD25+ Treg cells. © 2017 Elsevier Ltd
Li J.N.,The Second Peoples Hospital Of Yunnan Province |
Zhang Y.,Kunming Medical University |
Wang Y.F.,The Second Peoples Hospital Of Yunnan Province |
Chen J.Y.,Kunming Medical University
Genetics and Molecular Research | Year: 2017
Stem cell therapy faces many problems including poor survival rates and low viability. Enhancing the biological functions of stem cells improves efficacy of therapies. Estrogen, whose levels are elevated during pregnancy, affects the properties of bone marrow mesenchymal stem cells. Evidence suggests that adipose-derived stem cells (ADSCs), which are a type of adult mesenchymal stem cells, can be used in regenerative medicine. In fact, ADSCs from pregnant animals have been used in clinical therapies. However, the effect of the donor’s reproductive status on proliferation of ADSCs is unknown. We investigated the effect of 17b-estradiol (E2) and progesterone (P) on the in vitro proliferation of ADSCs from laboratory rats. ADSCs were obtained from five different groups of 15 rats each - non-pregnant, pregnant, in perinatal period, non-pregnant and treated with E2, and non-pregnant and treated with P. Adhesion and viability of ADSCs were determined by MTT assay, and cell cycle was followed by flow cytometry. The proliferation rate of ADSCs from pregnant rats was significantly higher than those from the non-pregnant rats (P < 0.05); however, there was no statistically significant difference in proliferation rates during different phases of pregnancy (P > 0.05). Additionally, ADSCs from pregnant rats possess higher adhesion property in early stage (P1 passage) and higher proliferation rate than ADSCs from non-pregnant rats. Interestingly, ADSCs from non-pregnant rats that were treated with E2, but not those treated with P, showed higher proliferation rates than those from their untreated counterparts. These results suggest that the proliferative capacity and residence time in different cell cycle phases of ADSCs can be regulated by extrinsic factors such as estrogen concentration. © 2017 The Authors.
Zhou Y.-H.,CAS Kunming Institute of Zoology |
Zhou Y.-H.,University of Chinese Academy of Sciences |
Liu F.-L.,CAS Kunming Institute of Zoology |
Liu F.-L.,University of Chinese Academy of Sciences |
And 7 more authors.
PLoS ONE | Year: 2011
Background: Co-infection with HIV and HCV and/or HBV is highly prevalent in intravenous drug users (IDUs). Because of the proximity to the "Golden Triangle", HIV prevalence among the IDUs is very high in the China-Myanmar border region. However, there are few studies about co-infection with HIV and HCV and/or HBV, especially in the region that belongs to Myanmar. Methods: 721 IDUs, including 403 Chinese and 318 Burmese, were investigated for their HIV, hepatitis B virus (HBV), and hepatitis C virus (HCV) serological status. Statistical analysis was performed to evaluate the differences of the epidemic situation between the Chinese IDUs and the Burmese IDUs. Results: Among the Chinese IDUs and the Burmese IDUs, HCV infection was the most prevalent (69.0% vs 48.1%, P<0.001), followed by HBV (51.6% vs 43.1%, P<0.05) and HIV (33.7% vs 27.0%, P>0.05). Besides, there were more HIV-HBV co-infected IDUs (20.1% vs 11.3%, P<0.005), and HIV-HCV co-infected IDUs (31.8% vs 23.9%, P<0.05) in China than in Myanmar, as well as HIV-HBV-HCV triple infection (19.1% vs 10.4%, P<0.005). Conclusion: Co-infection with HIV and HCV and/or HBV is highly prevalent among the IDUs in the China-Myanmar border region. The HIV epidemic appears to be in a downward trend, compared with previous reports. However, all infections were more prevalent among the Chinese IDUs than among the Burmese. © 2011 Zhou et al.
Li Y.,The Second Peoples Hospital of Yunnan province |
Yang Y.,The Second Peoples Hospital of Yunnan province |
Shi L.,Peking Union Medical College |
Li X.,The Second Peoples Hospital of Yunnan province |
And 2 more authors.
Diabetes/Metabolism Research and Reviews | Year: 2012
Adiponectin, which is secreted by the white adipose tissue, plays an important role in type 2 diabetes mellitus (T2DM) and its complications. Since 2002, many investigators explored the association between ADIPOQ single nucleotide polymorphisms and T2DM in different ethnic populations from different regions. In China, the results of numerous studies of the association between ADIPOQ and T2DM were not consistent, which may be caused by population-specific effects or environmental effects. This review describes the association between ADIPOQ and T2DM, the metabolic characteristics and the complications of T2DM in Chinese populations. © 2012 John Wiley & Sons, Ltd.
Li Y.,The Second Peoples Hospital of Yunnan Province |
Li X.,The Second Peoples Hospital of Yunnan Province |
Shi L.,Peking Union Medical College |
Yang M.,The Second Peoples Hospital of Yunnan Province |
And 5 more authors.
PLoS ONE | Year: 2011
Recently, many studies have reported that the SNP+45(T>G) and SNP+276(G>T) polymorphisms in the adiponectin gene are associated with type 2 diabetes (T2DM) in the Chinese Han population. However, the previous studies yielded many conflicting results. Thus, a meta-analysis of the association of the adiponectin gene with T2DM in the Chinese Han population is required. In the current study, we first determined the distribution of the adiponectin SNP+276 polymorphism in T2DM and nondiabetes (NDM) control groups. Our results suggested that the genotype and allele frequencies for SNP+276 did not differ significantly between the T2DM and NDM groups. Then, a meta-analysis of 23 case-control studies of SNP+45, with a total of 4161 T2DM patients and 3709 controls, and 11 case-control studies of SNP+276, with 2533 T2DM patients and 2212 controls, was performed. All subjects were Han Chinese. The fixed-effects model and random-effects model were applied for dichotomous outcomes to combine the results of the included studies. The results revealed a trend towards an increased risk of T2DM for the SNP+45G allele as compared with the SNP+45T allele (OR = 1.34; 95% CI, 1.11-1.62; P<0.01) in the Chinese Han population. However, there was no association between SNP+276 and T2DM (OR = 0.90; 95% CI, 0.73-1.10; P = 0.31). The results of our association study showed there was no association between the adiponectin SNP+276 polymorphism and T2DM in the Yunnan Han population. The meta-analysis results suggested that the SNP+45G allele might be a susceptibility allele for T2DM in the Chinese Han population. However, we did not observe an association between SNP+276 and T2DM. © 2011 Li et al.
PubMed | The Second Peoples Hospital of Yunnan Province, Honghe Center Blood Station and Kunming University of Science and Technology
Type: | Journal: Oncotarget | Year: 2017
We investigated the association between single nucleotide polymorphisms (SNPs) in ALDH2, which has been associated with alcohol dependence and several types of diseases, and the risk of drug addiction in a Chinese Han population. In a case-control study that included 692 cases and 700 healthy controls, eight SNPs in ALDH2 were selected and genotyped using the Sequenom MassARRAY platform. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using unconditional logistic regression after adjusting for age and gender. We determined that rs671 is significantly associated with a 1.551-fold increased drug addiction risk (95% CI = 1.263-1.903; p < 0.001). In the genetic model analysis, we found that rs671 is associated with an increased risk of drug addiction under additive, dominant and recessive models (p < 0.001), while rs886205, rs441 and rs4646778 displayed a decreased drug addiction risk under additive and recessive model, respectively (p < 0.05). SNP rs671 remained significant after Bonferroni correction (p<0.00125). Additionally, we observed that haplotype GTCAC was associated with increased drug addiction risk (OR = 1.668; 95% CI, 1.328-2.094, p < 0.001); in contrast, ATCGC was a protective haplotype for drug addiction risk (OR = 0.444; 95% CI, 0.281-0.704, p < 0.001). Our findings showed that ALDH2 polymorphisms are significantly associated with the risk of drug addiction in the Chinese Han population.
Miao Y.-L.,Kunming Medical University |
Xiao Y.-L.,The Second Peoples Hospital of Yunnan Province |
Du Y.,Kunming Medical University |
Duan L.-P.,Kunming Medical University
World Journal of Gastroenterology | Year: 2013
AIM: To identify peripheral blood mononuclear cell (PBMC) gene expression profiles of ulcerative colitis (UC) patients, using oligonucleotide microarrays, to gain insights into UC molecular mechanisms. METHODS: The Human OneArray microarrays were used for a complete genome-wide transcript profiling of PBMCs from 12 UC patients and 6 controls. Differential analysis per gene was performed with a random variance model; t test and P values were adjusted to control the false discovery rate (5%). Gene ontology (GO) was deployed to analyze differentially expressed genes at significant levels between patients and controls to identify the biological processes involved in UC. RESULTS: Comparative analysis revealed that 4438 probes (4188 genes) were differentially expressed between the two groups, of which 3689 probes (3590 genes) were down-regulated whereas 749 probes (598 genes) were up-regulated. Many disregulated genes in our data have been reported by previous microarray studies carried out on intestinal mucosa samples, such as S100A8, CEACAM1 and S100A9. GO enrichment analysis revealed 67 high enrichment up-regulated categories and one significant down-regulated category. The up-regulated genes were mainly involved in immune and inflammatory response, cell cycle and proliferation, DNA metabolism and repair. CONCLUSION: Gene expression profiling of PBMCs from patients with UC has highlighted several novel gene categories that could contribute to the pathogenesis of UC. © 2013 Baishideng. All rights reserved.
Zhang H.,The Second Peoples Hospital of Yunnan Province
International Journal of Ophthalmology | Year: 2011
AIM: To estimate the therapeutic effect and safety of small incision phacoemulsification and IOL implantation on uveitis coexisting with cataract. METHODS: Phacoemulsification with small incision and IOL implantation were performed in 40 eyes of 32 patients through a small corneal incision, and the small pupil and posterior synechia were carefully handled, then the postoperative visual acuity and complications were retrospectively analyzed. RESULTS: Follow-up 1-13 months (7.6 months in average), all cases were achieved round pupil and visual acuity was improved. Posterior capsule ruptured was in 3 eyes and tridemia occurred in 2 eyes during surgery. Corneal edema, fibrous exudates in anterior chamber, posterior synechia and uveitis recurrence were the main postoperative complications. CONCLUSION: This method with a small corneal incision can protect iris from injury. It is a safe and effective method for management of uveitis coexisting with cataract, there is little inflammatory reaction, and visual acuity is satisfactory.
Tang H.-L.,The Second Peoples Hospital of Yunnan Province |
Fan L.-M.,The Second Peoples Hospital of Yunnan Province
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2015
Objective: To investigate the effect of recombinant human erythropoietin on immune function in mice with multiple myeloma. Methods: Multiple myeloma model mice were divided randomly into control group and treatment groups (low, middle and high doses). The rats in treatment groups were injected with recombinant human erythropoietin 100 μL (2.5, 5.0 and 10 mg/kg) via the tail vein for 30 days, and the rats in control group were injected isotonic saline solution of the same volume instead. After the experiment, the colorimetric MTT assay, neutral red method and ELISA kit were used to evaluate the proliferation of B and T lymphocytes, the phagocytic ability of macrophages and the content of serum TNF-α. Results: Compared with those in control group, different doses of recombinant human erythropoietin could significantly increase the proliferation of lymphocytes, increase the phagocytosis of macrophages and improve the production of serum TNF-α(P<0.05). Conclusion: Recombinant human erythropoietin can significantly improve immunoregulation of mice with multiple myeloma. ©, 2015, Xi'an Medical University. All right reserved.
PubMed | The Second Peoples Hospital of Yunnan Province and Central South University
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016
We investigated whether ABCB1 variants confer susceptibility to primary open-angle glaucoma and predict individual differences to latanoprost treatment.Between May 2013 and May 2015, 129 POAG patients enrolled in the Department of Ophthalmology, the Second Peoples Hospital of Yunnan Province were identified as the case group and 121 healthy individuals were included as the control group. Direct DNA sequencing was used to detect four ABCB1 gene polymorphisms, namely, -129T>C (rs3213619), 1236C>T (rs1128503), 2677G>T/A (rs2032582) and 3435C>T (rs1045642). All POAG patients received latanoprost eye drops once daily. The differences in intraocular pressure (IOP) and visual acuity (VA) before and 1 month after latanoprost treatment were compared in different SNPs genotypes.Statistically significant differences in genotype frequency were found in ABCB1 gene polymorphism 2677G>T/A and 3435C>T between the case group and the control group (both P<0.05). No significant difference in genotype frequency was found in -129T> C and 1236C>T between the two groups (both P>0.05). Importantly, ABCB1 gene 3435C>T polymorphism was associated with a remarkably reduced IOP and an improved VA in POAG patients before and after latanoprost eye drops treatment (both P < 0.05). However, no significant differences in IOP and VA were found in other three genotypes between the two groups (all P>0.05).Our results suggest that ABCB1 gene polymorphisms 2677G>T/A and 3435C>T may confer to associated with the susceptibility to POAG. The ABCB1 gene polymorphism 3435C>T may be linked to individual differences in response to latanoprost eye drops treatment in POAG patients.