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Ruan T.,Nanjing Medical University | He X.,Nanjing Medical University | Yu J.,The Second Peoples Hospital of Wuxi | Hang Z.,Nanjing Medical University
Oncology Letters | Year: 2016

Liver cancer, particularly hepatocellular carcinoma (HCC), is one of leading causes of cancer-related mortality worldwide. Upregulation of the evolutionary conserved Hippo signaling pathway has been observed in HCC patients, and Yes-associated protein 1 (YAP1) has been reported to play a key role in HCC tumorigenesis. microRNAs (miRNAs) are a family of small non-coding RNAs, usually 21-25 nucleotides in length, and are essential in the regulation of gene expression. Abnormal miRNA expression has been implicated in the initiation and progression of numerous forms of cancers, including liver cancer. Here, we report the identification of a novel miRNA, miR-186, and its functions as an HCC tumor suppressor. We observed that miR-186 was downregulated in several HCC cell lines, and that it directly targets YAP1 mRNA. Overexpression of miR-186 in HCC cells significantly downregulates YAP1 mRNA and protein levels, leading to downregulation of the Hippo signaling pathway, which in turn severely inhibits HCC cell migration, invasion and proliferation. Our study is the first to report the direct involvement of miR-186 in downregulating YAP1 and, more significantly, inhibiting HCC tumorigenesis, and supports the role miR-186 as a potential therapeutic target in treating liver cancer. © 2016, Spandidos Publications. All rights reserved. Source

Zhao Z.,Jiangsu University | Jin C.,The Second Peoples Hospital of Wuxi | Ding K.,Jiangsu University | Ge X.,Changzhou Wujin Chinese Medicine Hospital | Dai L.,Jiangsu University
Experimental Dermatology | Year: 2012

Abstract: Melanoblasts (MB) are also called melanocyte (MC) precursor cells. In recent years, people have successfully cultivated human and mouse MB. Previous studies have shown that EDN3 induces cultivated bird MC to re-differentiate into double potential progenitor cells of MB. However, no study has reported whether in vitro cultivated human MC can be dedifferentiated. Our research on MC that were purified and cultivated in vitro found that adding 10nm endothelin 1 (EDN1) (ET-1) to the MC medium without phorbol 12-myristate 13-acetate (PMA) induced a few MC to dedifferentiate and become a new type of cell. This new cell type was separated, purified, cloned and identified using multiple approaches. The results show that 88.7%, 8.69% and 2.5% of this new cell type were cells in the G0-G1, G2-M and S stages, respectively. The new cell type did not exhibit an apparent apoptotic peak, and its apoptotic rate was 0.09%. Stage I melanosomes were observed in the cytoplasm and were negative for the DOPA reaction. The cell surface antigen expression was positive for tyrosinase-related protein 2, negative or positive for c-kit and negative for S-100 and HMB45, showing that these cells were dedifferentiated MB of MC. Our findings provided evidence for atavism of mature human MC under certain conditions. © 2012 John Wiley & Sons A/S. Source

Xu T.,The Second Peoples Hospital of Wuxi | Xiao D.,The Second Peoples Hospital of Wuxi | Zhang X.,Central South University
Oncology Letters | Year: 2013

ECRG4 has been shown to be a candidate tumor suppressor in several tumors, but its role in head and neck cancer remains poorly understood. In the present study, the effect of ECRG4 on head and neck cancer was investigated in vitro and in vivo. pFLAG-CMV-2-ECRG4 was stably transfected into squamous cell carcinoma of the head and neck (SCCHN) M2 cell lines to overexpress the ECRG4 gene. Real-time PCR and western blot analysis were performed to detect gene and protein expression, respectively. An MTT assay and fow cytometric analysis were used to detect the growth of M2 cells. Matrigel™ invasion and scratch assays were applied to observe the invasion and migration of the cells. A tumorigenicity assay was applied to test the tumor growth and cervical lymph node metastasis in vivo. Based on the data, pFLAG-CMV-2-ECRG4 significantly increased the expression of ECRG4 in the M2 cells. The constructed plasmid inhibited cell proliferation and promoted cell cycle arrest and apoptosis in the M2 cells. The growth rate and metastasis of the tumor cells in xenografts were suppressed following the overexpression of ECRG4 in nude mice. These data suggest that ECRG4 plays a significant role in the regulation of growth and metastasis in SCCHN, providing new clues for the diagnosis and therapy of SCCHN. Source

Ren T.-L.,The Second Peoples Hospital of Wuxi | Yang C.-J.,The Second Peoples Hospital of Wuxi | Hang Y.-X.,The Second Peoples Hospital of Wuxi | Shi H.,Yancheng No. 1 Hospital | And 4 more authors.
Analytical Letters | Year: 2013

Adenosine deaminase plays a crucial role in the development and maintenance of the normal immune system. Although the determination of adenosine deaminase in serum may serve as a noninvasive diagnostic tool in evaluation of the active phase of systemic lupus erythematosus and the severity of this disease, detection of this analyte in a biologically complex mixture remains a major challenge. In this report, a colorimetric biosensor is reported for the determination of adenosine deaminase activity. The sensor employed DNAzyme as the signal transformation element which does not have a complicated label process. The proposed method was simple, rapid, and economical, allowing monitoring of adenosine deaminase to levels less than 0.1 U L-1. The simplicity of this device suggests it may be a candidate for the rapid screening of proteins in human serum and have wide applications in the field of clinical diagnosis. © 2013 Copyright Taylor and Francis Group, LLC. Source

Ren T.-L.,The Second Peoples Hospital of Wuxi | Han Z.-J.,The Second Peoples Hospital of Wuxi | Yang C.-J.,The Second Peoples Hospital of Wuxi | Hang Y.-X.,The Second Peoples Hospital of Wuxi | And 5 more authors.
Journal of Biochemical and Molecular Toxicology | Year: 2014

Rheumatoid arthritis (RA) is a chronic inflammatory disease with complex genetic factors. Single-nucleotide polymorphisms (SNPs) in the SLC22A4 gene have been previously reported to be associated with RA in Japanese but not European populations. This study further investigated the association of SLC22A4 polymorphisms, in particular slc2F1/slc2F2, with RA in the Chinese population, the largest Asian population. A total of 160 human subjects with 95 RA patients and 65 healthy controls were genotyped for slc2F1-G/A and slc2F2-C/T polymorphisms. The results showed that there was a significant difference in the genotype distribution of these two polymorphisms between the two groups. In addition, the presence of slc2F1 A allele and slc2F2 T allele carries a 1.93-fold and 2.14-fold increased risk for anticyclic citrullinated peptide (CCP) positivity, respectively. Overall, this study provided evidence that SLC22A4 gene polymorphisms played important roles in the etiology of RA in the largest Asian population, the Chinese population. © 2014 Wiley Periodicals, Inc. Source

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