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OBJECTIVES: The aim of this study was to explore the role of the p38 mitogen-activated protein kinase (p38MAPK)/p53 signaling pathway in injury to the intestinal mucosal barrier during severe acute pancreatitis (SAP). METHODS: Both sham operation and SAP groups had 3 subgroups analyzed 3, 6, or 12 hours after the SAP induction. The concentrations of amylase, endotoxin, diamine oxidase, tumor necrosis factor α, and phospho-p38MAPK, p53, and caspase-3 and the messenger RNA levels of zonula occludens protein-1 and occludin in the intestine were measured. Immunohistochemical staining was used to determine the expression of zonula occludens protein-1 and occludin. Pathological changes of the pancreas and intestine were also assessed. Then, rats were randomly assigned to 5 groups—sham operation group, SAP group, 3 groups treated with different concentrations of p38MAPK-inhibitor SB203580—and the abovementioned experiment was repeated and analyzed 6 hours after the SAP induction. RESULTS: The phospho-p38MAPK reached a peak value at 6 hours after the SAP induction with obvious pathological injury to the pancreas and intestine. Treatment with SB203580 led to a less damage to the pancreatic and intestinal tissues. CONCLUSIONS: These results suggest that SAP activates the p38MAPK/p53 signaling pathway and induces injury to the intestinal mucosal barrier, which can be alleviated by inhibiting the p38MAPK/p53 pathway. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved. Source


Xu W.-Q.,the Second Peoples Hospital of Taizhou | Qi G.-Q.,the Second Peoples Hospital of Taizhou | Chen X.-Y.,the Second Peoples Hospital of Taizhou | Qi W.-Y.,the Second Peoples Hospital of Taizhou | And 2 more authors.
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014

Objective: To investigate the effects of drug treatment alone or drug combined with modified electroconvulsive therapy (MECT) on psychotic symptoms and cognitive function of patients with schizophrenia. Methods: One hundred and four inpatients with first-episode schizophrenia were divided into the low age group (age 18-26 years, n=52) and high age group (age 39-46 years, n=52) according to their ages. Each group was further divided into the drug treatment group and drug combined with MECT group based on the therapeutic method. The positive and negative syndrome scale (PANSS) score and Wisconsin card sorting test (WCST) were used to assess the psychotic symptoms and cognitive function for patients of each group before treatment and after 3, 6, and 12 weeks of treatment. The results were statistically analyzed. Results: The reduction of PANSS scores of the drug combined with MECT group of low age group were significantly higher than those of the drug treatment group after three weeks of treatment. The difference was statistically significant (P<0.001). The reduction of PANSS scores of the drug combined with MECT group of high age group were significantly higher than those of the drug treatment group after three weeks and six weeks of treatment. The difference was statistically significant (P<0.001). The number of responses and correct responses and completed categories of WCST of the drug combined with MECT group of low age group were significantly higher than those of the drug treatment group after three weeks of treatment. The number of continuous errors of the drug combined with MECT group of high age group was significantly lower than those of the drug treatment group. The differences were statistically significant (P<0.05). Conclusion: The MECT can rapidly improve the psychotic symptoms and increase the cognitive function of patients of different ages. Source

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