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Guo K.,Anhui Medical University | Lan C.-Z.,Anhui University of Traditional Chinese Medicine | Yu T.-T.,Anhui Medical University | Huang L.-L.,Anhui Medical University | And 3 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Xin-Ji-Er-Kang (XJEK), a Chinese herbal formula, is effective against hypertension induced coronary heart disease, viral myocarditis and toxic myocarditis. In this study, the effect of XJEK on cardiovascular system was investigated. To test the hypothesis that Xin-Ji-Er-Kang (XJEK) has an anti-hypertensive effect mediated through attenuation of cardiac remodeling, and amelioration of vascular endothelial dysfunction and oxidative stress. Materials and methods Hypertension was induced in Wistar rats by 2 kidney 1 clip (2K1C) treatment. The hypertensive rats were then randomly assigned into four groups and treated as follows: group 1 (Sham-operated [Sh-Op] group received only drinking water), group 2 (induced hypertensive model+no treatment), and group 3 (induced hypertensive+a single daily oral dose of 24 g kg-1 XJEK treatment) and group 4 (induced hypertensive+a single oral dose of 15 mg kg-1 Fosinopril treatment). The rats in all the defined groups were respectively treated for a period of 4 weeks. Cardiovascular parameter such as systolic blood pressure (SBP) was measured weekly by using tail-cuff apparatus; left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and the rate of the rise in left ventricular pressure (±dp/dt max) were measured by using a PowerLab 8/30 apparatus (AD Instruments, Australia) at the end of the 8th week; heart weight/body weight (HW/BW) was determined as an index of myocardial hypertrophy (MH). Hematoxylin and eosin (H&E) and Van Gieson (VG) stain were used to assess the cardio-histological changes. Colorimetric analysis was used to assay serum superoxide dismutase (SOD) activity, malondialdehyde (MDA), nitric oxide (NO), and hydroxyproline (Hyp) contents in cardiac tissue. Angiotensin II (Ang II) content in serum was assessed by radioimmunoassay; tetrahydrobiopterin (BH4) content in cardiac tissue, BNP and endothelial NOS (eNOS) in serum were determined by using ELISA, and the protein expressions of c-Jun NH2-terminal kinase (JNK), P-JNK, p38, P-p38, and NADPH oxidases-2 (Nox-2) were measured by western blot. Results XJEK therapy could impair the heart systolic and diastolic function, potently improve the heart weight index, inhibit the elevation of HW/BW ratio, and markedly ameliorate hemodynamic indices and vascular remodeling index. It has blunted the decrease of SOD, NO and the increase in MDA and Ang II serum contents, myocardial cross-section area (CSA), collagen volume fraction (CVF) and perivascular circumferential collagen area (PVCA) compared to the hypertensive model group. It also reduced the serum content of Hyp while increased BH4 levels in cardiac tissue. In addition, the expressions of Nox-2, P-JNK and P-p38MAPK were all suppressed compared to the hypertensive model group. Moreover, treatment with XJEK improved endothelial dysfunction (ED) manifested by promoting eNOS activities and enhancing the NO activity in serum. Conclusion The results of the present study show that XJEK attenuates 2K1C-induced hypertension in rats, which confirms our hypothesis that XJEK has an anti-hypertensive and cardiovascular remodeling effect via attenuation of cardiac remodeling and improvement of endothelial dysfunction and oxidative stress. © 2014 Elsevier Ireland Ltd.


Yuan H.,The Second Peoples Hospital Of Hefei City | Xia Q.,The Second Peoples Hospital Of Hefei City | Cao X.,The Second Peoples Hospital Of Hefei City | Wang X.,The Second Peoples Hospital Of Hefei City | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

The Angiotensin-converting enzyme (ACE) has been involved in the sporadic Alzheimer‘s disease (SAD) risk and investigated in numerous epidemiologic studies. ACE gene (SNPs rs4291A>T, rs4343A>G, and rs1800764T>C) polymorphisms may influence the risk for SAD. However, results thus far have been inconclusive. The purpose of the present study is to firstly investigate whether these three polymorphisms facilitate the susceptibility to SAD by a meta-analysis. The PubMed and EMBASE databases were searched for genetic association studies on three polymorphisms and SAD risk. 48 comparisons were identified and a meta-analysis was performed to evaluate the association between three polymorphisms and SAD risk by calculating combined odds ratios (ORs) and 95% confidence intervals (CIs). The combined results showed no significant association was found for rs4343A>G, rs4291A>T, and rs1800764C>T polymorphisms. However, the sensitivity analysis did not support the results for rs4921A>T (AA versus AT+TT: OR = 1.09, 95% CI: 1.01-1.18, P = 0.03 and AA versus AT: OR = 1.09, 95% CI: 1.00-1.18, P = 0.05). In summary, the meta-analysis results suggest rs4291 AA genotype within ACE gene is a risk factor for SAD and interactions might effect on ACE rs4291A>T genetic risk of SAD. However, the rs4343A>G or rs1800764T>C of ACE gene is not associated with SAD risk. © 2016, E-Century Publishing Corporation. All rights reserved.


Pan J.,The Second Peoples Hospital Of Hefei City | Gao C.,The Second Peoples Hospital Of Hefei City
International Journal of Clinical and Experimental Medicine | Year: 2016

The MCP-1 A2518G polymorphism has been indicated to be correlated with coronary artery disease (CAD) susceptibility, but the results of studies are still debatable. Thus, a meta-analysis was carried out. The databases of PubMed, Embase, Web of Science, and CNKI (China National Knowledge Infrastructure) were searched. The related data were extracted and pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated. At last, 21 case-control studies with 8,480 cases and 1,2366 controls were included in this meta-analysis. Overall, the significant association was showed (G allele vs. A allele: OR = 1.11, 95% CI: 1.02-1.21, P = 0.000; AG + GG vs. AA: OR = 1.14, 95% CI: 1.01-1.29, P = 0.000) between MCP-1 A2518G polymorphism and CAD risk. However, the evidence of heterogeneity was found among the investigated studies, and subgroup analyses were conducted according to source of population in control, ethnicity, types of CAD end points, and status of HWE. In conclusion, the meta-analysis suggests the MCP-1 A2518G polymorphism (AG + GG genotypes) is a risk factor for CAD. Further large-scale and well-designed studies are still needed to confirm the results of our meta-analysis. © 2016, E-Century Publishing Corporation. All rights reserved.


Dong S.-J.,The Second Peoples Hospital Of Hefei City | Cai X.-J.,The Second Peoples Hospital Of Hefei City | Li S.-J.,The Second Peoples Hospital Of Hefei City
Medical Science Monitor | Year: 2016

Background: 5-Fluorouracil (5-FU) based treatment is the standard therapy for metastatic colorectal cancer (CRC), but the development of chemoresistance is inevitable. Increasing evidence shows that dysregulation of microRNAs (miRNAs) is involved in malignant transformation. Thus, it is imperative that we find new diagnostic and prognostic marker for chemotherapy in CRC. Material/Methods: For clinical parameter analysis, 78 CRC tissues and adjacent normal tissues and 45 serum specimens from CRC patients were included in this study. For chemo-response analysis, 116 primary tissues were collected from the patients receiving first-line 5-FU treatment. Quantitative Real-Time PCR (qRT-PCR) was used to detect microRNAs expression. Results: The expression of miR-429 was significantly increased in both serum and primary tissues from CRC patients, and enhanced miR-429 level was associated with tumor size, lymph node metastasis, and TNM stage. The diagnostic and prognostic values were also confirmed in CRC by using primary tissues. For patients receiving 5-FU-based treatment, miR-429 levels were significantly lower in responding group. The proportions of patients that did not experience response to therapy were higher in primary tumors with high miR-429 expression levels as compared with primary tumors with low miR-429 expression levels. Finally, Kaplan-Meier survival analysis showed that miR-429 is an independent prognostic indicator for chemo-response to 5-FU therapy among CRC patients. Conclusions: High level of miR-429 expression was correlated with enhanced malignant potential and poor prognosis of CRC patients. Furthermore, miR-429 could affect the chemo-sensitivity of CRC patients to 5-FU therapy and was associated with poor response to 5-FU-based chemotherapy in patients with CRC. © Med Sci Monit, 2016.


PubMed | The Second Peoples Hospital of Hefei City
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016

BACKGROUND 5-Fluorouracil (5-FU) based treatment is the standard therapy for metastatic colorectal cancer (CRC), but the development of chemoresistance is inevitable. Increasing evidence shows that dysregulation of microRNAs (miRNAs) is involved in malignant transformation. Thus, it is imperative that we find new diagnostic and prognostic marker for chemotherapy in CRC. MATERIAL AND METHODS For clinical parameter analysis, 78 CRC tissues and adjacent normal tissues and 45 serum specimens from CRC patients were included in this study. For chemo-response analysis, 116 primary tissues were collected from the patients receiving first-line 5-FU treatment. Quantitative Real-Time PCR (qRT-PCR) was used to detect microRNAs expression. RESULTS The expression of miR-429 was significantly increased in both serum and primary tissues from CRC patients, and enhanced miR-429 level was associated with tumor size, lymph node metastasis, and TNM stage. The diagnostic and prognostic values were also confirmed in CRC by using primary tissues. For patients receiving 5-FU-based treatment, miR-429 levels were significantly lower in responding group. The proportions of patients that did not experience response to therapy were higher in primary tumors with high miR-429 expression levels as compared with primary tumors with low miR-429 expression levels. Finally, Kaplan-Meier survival analysis showed that miR-429 is an independent prognostic indicator for chemo-response to 5-FU therapy among CRC patients. CONCLUSIONS High level of miR-429 expression was correlated with enhanced malignant potential and poor prognosis of CRC patients. Furthermore, miR-429 could affect the chemo-sensitivity of CRC patients to 5-FU therapy and was associated with poor response to 5-FU-based chemotherapy in patients with CRC.


PubMed | the Second Peoples Hospital of Hefei City
Type: | Journal: The International journal of neuroscience | Year: 2015

Stroke is a multifactorial disease in which genetic factors play an important role. Previous studies associated angiotensin converting enzyme (ACE) (insertion/deletion, I/D) gene polymorphism with ischemic stroke risk in Caucasian individuals reported conflicting results. The purpose of this study was to evaluate the association between ACE (I/D) gene polymorphism and ischemic stroke risk by a meta-analysis.The related studies were searched in MEDLINE, EMBASE, and HuGEnet databases. The odds ratios (ORs) and corresponding 95% confidence intervals (CIs) for ischemic stroke risk associated with this polymorphism were estimated using fixed-effect or random-effects model. Twenty-two studies (5528/5081 cases/controls) were eligible in our meta-analysis.Overall, statistical associations of the ACE (I/D) polymorphism with ischemic stroke risk were found in dominant model (DD+ID versus II) : OR = 1.21, 95%CI = (1.06,1.38), P = 0.006, recessive model (DD versus ID+II): OR = 1.28, 95%CI = (1.05,1.55), P = 0.01, and homozygote comparison (DD versus II): OR = 1.37, 95%CI = (1.14,1.65), P = 0.001 for Caucasians. When stratifying according to stroke subtypes, there were similarly significant differences for small vessel disease in dominant model (DD+ID versus II) : OR = 1.44, 95%CI = (1.01,2.05), P = 0.04, recessive model (DD versus ID+II): OR = 1.30,95%CI = (1.09,1.55), P = 0.004, and homozygote comparison (DD versus II): OR = 1.44, 95%CI = (1.15,1.80), P = 0.001.This analysis suggests that the ACE (I/D) polymorphism may be a risk factor for ischemic stroke, genotype DD of ACE could increase the risk of ischemic stroke in Caucasians. Subgroup analyses indicate that stroke subtypes may be a genetic risk factor of ischemic stroke, and there might be a greater genetic liability with small vessel disease.


PubMed | The Second Peoples Hospital of Hefei City
Type: Journal Article | Journal: Molecular biology reports | Year: 2013

A large number of epidemiological studies have been performed to investigate the association between Alzheimers disease (AD) risk and interleukin-1 -511C/T genetic polymorphism, however, inconsistent results have been reported. The effect of the IL-1 -511C/T polymorphism on AD susceptibility was evaluated by a meta-analysis. Series of databases were researched. 14 studies involving 2640 AD case and 3493 control subjects were identified. The pooled results showed there were no statistical associations of interleukin-1 -511C/T genetic polymorphism with susceptibility to AD for five analysis models in all subjects. However, obvious heterogeneity among studies was detected. When stratifying for age at onset, ethnicity and geographic distribution of population to explore the original source of heterogeneity, the meta-analysis results based on geographic distribution of population showed the significant difference (CC vs CT, OR 1.26, 95% CI: 1.03, 1.54, z=2.25, P=0.025; CC vs CT+TT, OR 1.24, 95% CI: 1.03, 1.50, z=2.24, P=0.025) only in non-Europe. These findings indicate that the IL-1 -511C/T polymorphism might be associated with AD risk, and individuals with IL-1 -511C/C genotype might be at higher risk of AD in non-Europe. Further larger sample research would be warranted to confirm these conclusions.

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