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Wei X.,Central South University | Li Q.,Central South University | Han Z.,The Second Peoples Hospital of Hainan Province | Lin D.,The Second Peoples Hospital of Hainan Province | Yu P.,Central South University
International Journal of Clinical and Experimental Medicine | Year: 2015

This study’s objectives are to assess the efficacy of detecting apoptotic caspase-3, -8, and -9 in human sperm and plasma using enzyme-linked immunosorbent assays (ELISA), and to compare these levels between fertile and infertile patient groups of Li nationality in China. This study offers a non-invasive, alternative strategy to analyzing sperm parameters in infertile males. Fifty-six infertile males were investigated; asthenospermia (n = 19), oligoasthenoteratozoospermia (n = 20), azoospermia (n = 17) compared with 20 healthy fertile controls. They were subjected to semen analysis by computer-assisted sperm assay (CASA). We found that caspase-3, -8, -9 existed in all specimens in both sperms and plasma. The level of caspase-3 and caspase-8 in plasma were both significantly higher than in sperm. Levels of caspase-8 and caspase-9 in sperm and plasma were significantly negatively correlated with sperm concentration, motility and A % (motility grade A). The level of caspase-8 in plasma was significantly negatively correlated with sperm concentration. However, only in healthy fertile controls sperm concentration was significantly negatively correlated with caspase-9 in sperm. Compared with the healthy fertile controls, only the OAT group exhibited significantly increased level of caspase-8 in sperm (P < 0.05). It is concluded that caspase-8 and caspase-9 in sperm and plasma are correlated with sperm motility, and can reflect the quality of sperm in vitro. © 2015, Int J Clin Exp Med. All rights reserved. Source


Zhou H.-L.,The Second Peoples Hospital of Hainan Province | Li L.-D.,The Second Peoples Hospital of Hainan Province | Wang P.,The Second Peoples Hospital of Hainan Province
International Eye Science | Year: 2014

AIM: To determine the group distribution characteristics of lachrymal duct obstruction diseases in major Li Miao minority areas of Hainan province. METHODS: Totally 5 353 residents were selected and researched by randomized cluster sampling in the major Li Miao minority areas of Hainan province. Ocular examination and lachrymal duct flushing were carried out, and questionnaire survey on lachrymal duct obstruction was conducted. The ratio of lachrymal duct obstruction diseases and group distribution characteristics were analyzed based on above research. RESULTS: The prevalence ratios of lachrymal duct obstruction was 4.47% in major Li Miao minority areas of Hainan province, with 2.62% in urban area, and 5.93% in rural area respectively. Prevalence ratios of men and women group were 1.69% and 6.39% correspondingly. Difference between the two groups was statistically significant (χ2=67.2821, P=0.0000). The highest prevalence ratio was 40-69 year-old group, second one was 70-79 year-old group, especially for women in these groups. The prevalence ratios of Ledong, Lingshui, Baisha and Changjiang county were higher than those of Baoting, Qiongzhong county and Wuzhishan city. No significant difference was found between both eyes. CONCLUSION: In major Li Miao minority areas of Hainan province, lachrymal duct obstruction mainly occurs in 40-79 year-old patients, with specially higher ratio of women. Statistically, significant difference of the prevalence ratio between urban and rural areas exists. The higher prevalence ratio is attributed to age, gender, geographical location, climate condition, health environment and so on. The prevalence ratio is higher in the dry and windy areas than in the humid and less windy areas. Copyright 2014 by the IJO Press. Source


Wei X.,Central South University | Han Z.,The Second Peoples Hospital of Hainan Province | Ren B.,Central South University | Xiao X.,Central South University | And 7 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: To investigate the presence of anti-sperm antibodies (AsAb) and the correlation between AsAb positivity and the expression of soluble major histocompatibility complex class I chain-related A and B (sMICA or sMICB) in the sera of infertile people of the Li nationality from Hainan, China. Method: A total of 136 people (68 couples) from five villages in the Wuzhishan region, Hainan province participated in this study. Among them, 31 couples were included in the fertile group and 37 couples in the infertile group. AsAb and sMICA/sMICB levels in serum were detected by ELISA. The median sMICA/sMICB levels between and among groups were compared by Mann-Whitney rank U testing and Kruskal-Wallis H testing, and the AsAb positivity rate was compared by Pearson Chi-Square testing. Correlation analysis was performed by calculating the Spearman’s rho coefficient for nonparametric data. Results: The serum levels for the fertile group (AsAb: 15.5 [4.0~127.0] U/ml, sMICA: 18.33 [13.30~52.40] pg/ml, sMICB: 27.72 [18.63~47.43] pg/ml) were not statistically different from those for the infertile group (AsAb: 18.0 [9.8~95.0] U/ml, sMICA: 20.95 [15.78~23.81] pg/ml, sMICB: 26.26 [18.06~61.38] pg/ml). However, grouping based on AsAb positivity revealed a statistically significant difference for the sMICA/sMICB levels (AsAb positive group: sMICA: 5.56 [4.30~17.23] pg/ml, sMICB: 16.13 [7.54~25.43] pg/ml; AsAb negative group: sMICA: 22.00 [18.05~66.13] pg/ml, sMICB: 36.51 [20.53~67.22] pg/ml; P < 0.01). These results suggest that AsAb is negatively associated with both sMICA (Spearman’s coefficient, -0.475, P < 0.01) and sMICB (Spearman’s coefficient, -0.381; P < 0.01). The analysis also shows that sMICA levels are positively associated with sMICB levels (Spearman’s coefficient, 0.635; P < 0.01). Conclusion: AsAb can be detected in the serum of fertile and infertile Li people. However, there appears to be limited clinical value in the conventional detection of AsAb, sMICA and sMICB in serum for diagnosing infertility. People with positive AsAb expression have lower levels of sMICA/sMICB expression in serum, which may be one mechanism by which people produce AsAb. © 2015, E-Century Publishing Corporation. All Rights Reserved. Source

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