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Li Y.-Q.,The Second Hospital Of Xian Jiaotong University | Xue J.-H.,The Second Hospital Of Xian Jiaotong University | Zhang Y.,The Second Hospital Of Xian Jiaotong University | Gao D.-F.,The Second Hospital Of Xian Jiaotong University | And 2 more authors.
Experimental and Therapeutic Medicine | Year: 2015

The adhesion of monocytes to endothelial cells is one of the early stages in the development of atherosclerosis. The expression of type IV collagenases, which include matrix metalloproteinase (MMP)-2 and MMP-9, in monocytes is hypothesized to play an important role in monocyte infiltration and transformation into foam cells. The aim of the present study was to examine the effects of monocyte-endothelium interactions on the expression levels of type IV collagenases and their specific inhibitors in monocytes, and to investigate the roles of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in this process. Monocytes were single-cultured or co-cultured with endothelial cells. The expression of the type IV collagenases, MMP-2 and MMP-9, and their specific inhibitors, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2, in monocytes was determined by immunohistochemistry followed by image analysis. The expression levels of MMP-2 and MMP-9 were found to be low in the single-culture monocytes, but increased significantly when the monocytes and endothelial cells were co-cultured. However, treatment with monoclonal TNF-α or IL-1β antibodies partially inhibited the upregulated expression of MMP-2 and MMP-9 in the co-cultured monocytes. Expression of TIMP-1 and TIMP-2 was observed in the single monocyte culture, and a small increase in the expression levels was observed when the monocytes were co-cultured with endothelial cells. Therefore, monocyte-endothlium interactions were shown to increase the expression of type IV collagenases in monocytes, resulting in the loss of balance between MMP-2 and -9 with TIMP-1 and -2. In addition, TNF-α and IL-1β were demonstrated to play important roles in this process. © 2014, Spandidos Publications. All rights reserved.

Wu J.W.,The Second Hospital Of Xian Jiaotong University | Xiao S.X.,The Second Hospital Of Xian Jiaotong University | Huo J.,The Second Hospital Of Xian Jiaotong University | An J.G.,The Second Hospital Of Xian Jiaotong University | Ren J.W.,The Second Hospital Of Xian Jiaotong University
Archives of Dermatological Research | Year: 2014

Multiple familial trichoepithelioma (MFT) (OMIM: 601606) is an autosomal dominantly inherited disorder characterized by numerous, skin-colored papules and nodules with pilar differentiation. Recently, several mutations in the cylindromatosis (CYLD) gene have been reported in MFT. In this study, a mutation analysis of the CYLD was conducted in a Chinese pedigree of typical MFT. Affected individuals were identified through probands from Shanxi Province, China. Lesional skin biopsy of the proband revealed the typical histopathological characteristics of trichoepithelioma. Individuals belonging to five consecutive generations were similarly affected, which indicated an autosomal dominant inheritance pattern. Genomic DNA was extracted from peripheral blood lymphocytes using standard phenol/chloroform extraction method. All the coding exons (4–20) and exon–intron boundaries of the CYLD gene were amplified by polymerase chain reaction (PCR). Direct sequencing of all PCR products amplified from the complete coding regions of the CYLD gene was performed to identify mutations. Sequencing of the CYLD gene was performed in a further 100 unrelated, unaffected control individuals to exclude the possibility of polymorphism. A novel heterozygous frameshift mutation c.1169_1170delCA (p.Thr390Argfs) was identified in exon 10 of the CYLD gene in the affected family members. This mutation was also detected in unaffected family members, but not in the unrelated, healthy individuals who were also analyzed. Our study expands the database on the CYLD gene mutations in MFT and should be useful in providing genetic counseling and prenatal diagnosis for families affected by MFT. © 2014, Springer-Verlag Berlin Heidelberg.

Li D.H.,The Fourth Hospital of Xian | Li X.P.,The Fourth Hospital of Xian | Zhang S.C.,The Fourth Hospital of Xian | Wu J.F.,The Second Hospital Of Xian Jiaotong University | Qi Y.H.,The Second Hospital Of Xian Jiaotong University
Genetics and Molecular Research | Year: 2016

Despite more than a century of intensive study, the mechanisms of successful pregnancy remain unclear. Recent research suggests that NF-κB (nuclear factor kappa B) plays an important role in embryo implantation. In the current study, we aimed to identify SNPs that contribute to genetic susceptibility for recurrent implantation failure (RIF). Thus, we examined the potential associations between RIF and ten SNPs (rs28362491, rs3774932, rs1598856, rs230528, rs230521, rs3774956, rs4648055, rs3774964, rs4648068, and rs3774968) of the NF-κB gene. Participants included 209 patients with RIF and 395 controls. Our results revealed that there were statistically significant differences observed in the allelic and genotypic frequencies of the rs28362491 promoter in the NF-κB gene. The frequency of the del/ del genotype was significantly higher in RIF patients than in healthy controls (P = 0.004). Compared with healthy controls, the RIF patients carried a higher frequency of the rs28362491 del allele (P = 0.010). Furthermore, strong linkage disequilibrium was observed in the three identified haplotype blocks (D’ > 0.9). Particularly, in block 1 (rs230528-rs230521), the A-C haplotype occurred significantly more frequently (P = 0.029) in subjects with RIF (P = 0.0003). In contrast, the A-G haplotype occurred significantly less frequently (P = 0.008) in RIF subjects. These findings support an important role for G-712A polymorphisms of NF-κB in RIF, and may guide future studies that aim to characterize genetic risk factors for RIF. © FUNPEC-RP.

Yang J.,The Second Hospital Of Xian Jiaotong University | Liu N.,The Second Hospital Of Xian Jiaotong University | Kang A.-J.,The Second Hospital Of Xian Jiaotong University | Zhao S.-P.,The Second Hospital Of Xian Jiaotong University | And 4 more authors.
World Chinese Journal of Digestology | Year: 2012

AIM: To investigate the expression of transmembrane protein 16A (TMEM16A) in colorectal carcinoma. METHODS: The expression of TMEM16A was detected by immunohistochemistry in 67 surgical colorectal carcinoma specimens and matched tumor-adjacent colorectal specimens. RESULTS: TMEM16A was expressed in both cytoplasm and cell membrane. Among 67 colorectal carcinoma specimens, TMEM16A expression was negative in 4 cases (5.97%), weakly positive in 11 cases (16.42%), positive in 20 cases (29.85%), and strongly positive in 32 cases (47.76%). Among 67 tumor-adjacent healthy tissue specimens, TMEM16A expression was negative in 27 cases (40.30%), weakly positive in 35 cases (52.24%), positive in 3 cases (4.48%), and strongly positive in 2 cases (2.99%). The positive ("positive" plus "strongly positive") rate of TMEM16A expression was significantly higher in colorectal carcinoma tissue than in tumor adjacent healthy tissue (77.61% vs 7.46%, P < 0.005). CONCLUSION: Aberrant expression of TMEM16A is a frequent feature in colorectal carcinoma. TMEM16A can be used as a new candidate target for diagnosis and treatment of colorectal carcinoma. © 2012, WJC Press. All rights reserved.

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