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Lei W.,Peoples Hospital Of Mianzhu | Liu Y.-E.,The PLA Second Artillery General Hospital | Zheng Y.,Third Peoples Hospital Of Chengdu | Qu L.,Design Institute of Chengdu Military Region
Medical Science Monitor | Year: 2015

Background: Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.Material/Methods: The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was downregulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.Results: MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.Conclusions: Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation. © Med Sci Monit.

Zhang Y.-K.,PLA Fourth Military Medical University | Zhang Y.-K.,The PLA Second Artillery General Hospital | Wang H.,Beijing Institute of Radiation Medicine | Leng Y.,Capital Medical University | And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2011

MicroRNAs (miRNAs) are small, noncoding ribonucleic acids (ncRNAs), which regulate gene expression by targeting mRNAs for translational repression and degradation. Several lines of evidences have indicated that miRNAs act as tumor suppressors and oncogenes. However, the role of miRNAs in pathogenesis of multiple myeloma (MM) remains unclear. In this study, we examined the profile of miRNA expression of primary MM cells, using miRNA microarray and quantitative real-time polymerase chain reaction (qPCR) techniques. These results showed that in the bone marrow specimens analyzed, miRNA-29b was significantly downregulated. Similar results were also observed in human myeloma cell lines (HMCLs). Adenovirus-mediated overexpression of miR-29b induced apoptosis and elevated caspase-3 activation in HMCLs. Using a bioinformatics approach, we found a perfect complementarity between miRNA-29b and the 3'UTR of myeloid-cell-leukemia 1(Mcl-1). It is further confirmed that miRNA-29b downregulated the level of Mcl-1 without effect on the mRNA level using both qRT-PCR assays and Western blot analyses. Moreover, we observed that enforced miR-29b expression by using a retarget miRNA-29b expression vector (Ad5F11p-miR-29b) could induce apoptosis and elevate caspase-3 activation in HMCLs. Our results also indicated that miRNA-29b-induced apoptosis acted antagonistically with IL-6 in HMCLs. These findings suggest that miRNA-29b may play an important role in MM as a tumor suppressor. © 2011 Elsevier Inc.

Zhang Y.,The PLA Second Artillery General Hospital | Li H.,The PLA Second Artillery General Hospital | Li S.,The PLA Second Artillery General Hospital | Su Z.,Beijing Institute of Radiation Medicine | And 2 more authors.
Qiangjiguang Yu Lizishu/High Power Laser and Particle Beams | Year: 2011

Male Babl/c mouses were exposed to 0.625 mW/cm 2 and 1.25 mW/cm 2 2856 MHz microwaves for 30 min×2 times. Venous blood samples were collected at 1 h, 1 d, 3 d, 7 d, 15 d and 30 d after exposure and differential counting of blood cells were performed. Immediate increases in white blood cell count and percentage, neutrophilic granulocyte count and percentage were observed after exposure to 0.625 mW/cm 2 and 1.25 mW/cm 2 microwave irradiations. On days 3, 7 and 15, they elevated more significantly than control group (p≤0.01). Lymphocyte count and percentage decreased after exposure to both irradiations. On days 3, 7 and 15, they reduced more significantly than control group (p≤0.01). Both 0.625 mW/cm 2 and 1.25 mW/cm 2 dose exposures have little effect on, platelets and erythrocytes. Low dose electromagnetic radiations could increase the white blood cell count and the neutrophilic granulocyte count, while reduce the lymphocyte count in the body.

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