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PubMed | Shanghai JiaoTong University, The Second Affiliated Hospital of Kunming Medical College, Tianjin Medical University, Central South University and 4 more.
Type: Journal Article | Journal: Journal of diabetes investigation | Year: 2016

To assess the efficacy and safety of acetyl-L-carnitine (ALC) on diabetic peripheral neuropathy compared with methylcobalamin (MC).This was a multicenter, randomized, parallel-group, double-blind, double-dummy, positive-controlled, non-inferior phaseII clinical trial. Diabetic patients with abnormal nerve conduction test results were randomized in a 1:1 ratio to receive oral ALC 500mg t.i.d. or MC 0.5mg t.i.d. for 24weeks. The neuropathy symptom score, neuropathy disability score and neurophysiological parameters were measured during follow up.A total of 232 patients were randomized (ALC n=117, MC n=115), 88% of which completed the trial. At week24, patients from both groups had significant reductions in both neuropathy symptom score and neuropathy disability score with no significant difference between two groups (neuropathy symptom score reduction: ALC vs MC 2.352.23, P<0.0001 vs 2.112.48, P<0.0001, intergroup P=0.38; neuropathy disability score reduction ALC vs MC 1.661.90, P<0.0001 vs 1.351.65, P<0.0001, intergroup P=0.23). Neurophysiological parameters were also improved in both groups. No significant difference was found between groups in the development of adverse events.ALC is as effective as MC in improving clinical symptoms and neurophysiological parameters for patients with diabetic peripheral neuropathy over a 24-week period with good tolerance.


PubMed | Washington University in St. Louis, Shenzhen University, Sun Yat Sen University, the Second Affiliated Hospital of Kunming Medical College and 4 more.
Type: | Journal: Scientific reports | Year: 2015

The phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway has been identified as an important pathway in renal cell carcinoma (RCC). We have reported a nonsense mutation in PIK3R1, which encodes the regulatory subunit of PI3K, in a metastatic RCC (mRCC), while the mutation was absent in the corresponding primary RCC (pRCC). To identify the function of PIK3R1 in RCC, we examined its expression in normal kidney, pRCC and mRCC by immunohistochemistry and real-time polymerase chain reaction. The expression of PIK3R1 significantly decreased in pRCC and was further reduced in mRCC compared with normal tissue. Besides, its expression levels were negatively correlated with T-category of tumor stage. Additionally, 786-O and A-704 cells with PIK3R1 depletion introduced by CRISPR/Cas9 system displayed enhanced proliferation, migration and epithelial-mesenchymal transition (EMT), and acquired a stem-like phenotype. Moreover, the PIK3R1 depletion promoted the phosphorylation of AKT in the cells. The knockdown of AKT by shRNA reduced p-GSK3 and CTNNB1 expression in the cells, while the depletion of CTNNB1 impaired stem-like phenotype of the cells. Overall, PIK3R1 down-regulation in RCC promotes propagation, migration, EMT and stem-like phenotype in renal cancer cells through the AKT/GSK3/CTNNB1 pathway, and may contribute to progression and metastasis of RCC.


Shen X.,Kunming University of Science and Technology | Sun D.,Kunming University of Science and Technology | Xie J.,The Second Affiliated Hospital of Kunming Medical College | Cai F.,Kunming University of Science and Technology | Li T.,The Second Affiliated Hospital of Kunming Medical College
Yingyong Lixue Xuebao/Chinese Journal of Applied Mechanics | Year: 2012

In this article, based upon the physiological data coming from the Human Medical literature, an approximate 3-dimensional geometric model for human spine of L3-L5 is established with the Solidedge which is a Computer-Aided Design software. With the finite element analysis software ANSYS, a more accurate human spine element model of L3-L5, which has the same material properties and contact characteristics with the true human spine, is built. The vertebra is divided into such structures as cortical bone and cancellous bone. The connections between vertebra and disc and between the small joints are contact. The finite element model is meshed using solid 187 element. An analysis of the mechanical properties under the 200N axial force and the 100N lateral force is made, the data of deformation and stress are obtained. This study provides believable data for the spinal biomechanics research and the correction of scoliosis spine.


Tao Y.,Peking Union Medical College | Chen J.,The Second Affiliated Hospital of Kunming Medical College | Yao Y.,Peking Union Medical College | Shi L.,Peking Union Medical College | And 4 more authors.
International Journal of Immunogenetics | Year: 2013

Summary: Recently, a 14-bp insertion/deletion polymorphism (+14 bp/-14 bp) in exon 8 of the Human leucocyte antigen-G (HLA-G) gene has been studied extensively because this polymorphism has been associated with HLA-G mRNA stability and could influence HLA-G mRNA expression. We investigated the distribution of the 14-bp insertion/deletion polymorphism in six different Chinese ethnic groups (Bulang, Wa, Hani, Jinuo, Maonan and Zhuang), which originated from three major ancient tribes (Di-Qiang, Baipu and Baiyue) in China. Comparison of the 14-bp insertion frequency in the six groups with other Chinese groups showed marked variation among the three ancient tribes, Di-Qing (0.490-0.534), Baipu (0.470-0.609) and Baiyue (0.280-0.344). Furthermore, the frequencies of the 14-bp insertion were similar in groups that came from the same ancient tribe, which indicated that the individuals who share the 14-bp insertion have the most probably inherited the 14-bp element from a common ancestor. In addition, an intra-tribal comparison of the 14-bp insertion/deletion frequencies between the descendants of the ancient ancestral tribes suggests that population histories or some environmental effects, such as founder effect or isolation, might also influence the distribution. © 2012 Blackwell Publishing Ltd.


Li C.,CAS Institute of Biophysics | Li C.,The Second Peoples Hospital of Shenzhen | Wu S.,The Second Peoples Hospital of Shenzhen | Wang H.,The Second Affiliated Hospital of Kunming Medical College | And 7 more authors.
Oncotarget | Year: 2015

Bladder cancer is one of the most common malignant tumors worldwide. Bladder cancer stem cells (BCSCs) have been isolated recently but have not been defined yet. Here we sorted BCSCs from bladder tumor tissues or normal bladder stem cells (NBBCs) from adjacent normal bladder tissues. We found that the C228T mutation (chr5, 1, 295, 228 C > T) of TERT promoter frequently occurs in BCSCs, but not exist in NBBCs. Importantly, introducing the C228T mutation in NBBCs causes TERT overexpression and transformation of bladder cancer. Restoration of the C228T mutation to T228C in BCSCs can recover the TERT expression to a basal level and abolish tumor formation. Additionally, the C228T mutation of TERT promoter triggers TERT expression leading to increased telomerase activity. TERT expression levels are consistent with clinical severity and prognosis of bladder cancer.


PubMed | CAS Institute of Biophysics, China Pharmaceutical University, Xi'an Jiaotong University, Dana-Farber Cancer Institute and The Second Affiliated Hospital of Kunming Medical College
Type: Journal Article | Journal: Theranostics | Year: 2017

Cancer stem cells (CSCs) are considered one of the key contributors to chemoresistance and tumor recurrence. Therefore, the precise identification of reliable CSC markers and clarification of the intracellular signaling involved in CSCs remains a great challenge in fields relating to cancer biology. Here, we implemented a novel chemoresistant prostate cancer patient-derived xenograft (PDX) model in NOD/SCID mice and identified CD54 as a candidate gene among the most highly enriched gene expression profiles in prostate tumors exposed to chronic cisplatin administration. Additional


PubMed | Yunnan Normal University, The Second Affiliated Hospital of Kunming Medical College, Yunnan University and Southwest forestry University
Type: | Journal: BioMed research international | Year: 2016

This paper presents an ultrasound simulation model for pulsatile blood flow, modulated by the motion of a stenosed vessel wall. It aims at generating more realistic ultrasonic signals to provide an environment for evaluating ultrasound signal processing and imaging and a framework for investigating the behaviors of blood flow field modulated by wall motion. This model takes into account fluid-structure interaction, blood pulsatility, stenosis of the vessel, and arterial wall movement caused by surrounding tissues motion. The axial and radial velocity distributions of blood and the displacement of vessel wall are calculated by solving coupled Navier-Stokes and wall equations. With these obtained values, we made several different phantoms by treating blood and the vessel wall as a group of point scatterers. Then, ultrasound echoed signals from oscillating wall and blood in the axisymmetric stenotic-carotid arteries were computed by ultrasound simulation software, Field II. The results show better consistency with corresponding theoretical values and clinical data and reflect the influence of wall movement on the flow field. It can serve as an effective tool not only for investigating the behavior of blood flow field modulated by wall motion but also for quantitative or qualitative evaluation of new ultrasound imaging technology and estimation method of blood velocity.


Bi H.,The Second Affiliated Hospital of Kunming Medical College | Ju J.,The Second Affiliated Hospital of Kunming Medical College
Chinese Journal of Gastroenterology | Year: 2012

The role of psychological factors in the pathogenesis of irritable bowel syndrome (IBS) has attracted more and more attention in clinical practice. With the increasing of the social pressure, symptoms in IBS patients could not be completely ameliorated by drug treatment only, and behavioral psychotherapy has some efficacy for the treatment of IBS. The aim of behavioral psychotherapy is not for complete cure of disease but to alleviate clinical symptoms of IBS patients, save medical expenditure and improve the quality of life. This article reviewed the advances in study on behavioral psychotherapy for IBS.


PubMed | The Second Affiliated Hospital of Kunming Medical College and Yunnan University
Type: Journal Article | Journal: Bio-medical materials and engineering | Year: 2016

The simulated Doppler blood flow signals are widely used to assess the performance of the clutter filters for removing wall components while reserving low-velocity signals scattered from physiological blood flow approaching the inner vessel-wall injured by a stenosed lesion.By simultaneously taking into account the natural attributes of the Doppler equipment, blood flow as well as vessel wall of pulsatile carotid arteries with a stenosis, a computer simulation method is presented to produce the compound Doppler ultrasound blood flow signals.The in-phase and quadrature (I/Q) axial as well as radial blood flow signals are simulated by superposing a series of cosine functions regulated by the spectrograms estimated from the axial and radial velocity profiles firstly obtained through the solution of the incompressible Navier-Stokes equations, respectively. Meanwhile, the I/Q Doppler signals echoed from pulsatile near (anterior) and far (posterior) walls are reproduced based on their radial movements during a cardiac cycle. Ultimately, those confirmed quadrature signals are summed to generate the compound Doppler signals including the contribution from both blood flow and stenosed vessel-wall.The compound Doppler ultrasound signals echoed from both axial and radial blood flows as well as vessel walls with obstruction grades of 0% (normal arteries), 10% and 25% are simulated respectively. The real signals from the left carotid artery with an approximately 10% stenosis degree are also collected for further assessing the believability of simulated versions.The simulated and clinical tests demonstrate that the proposed computer simulation method can produce compound Doppler signals with confirmed qualitative and quantitative characteristics resembled with the clinical versions, which could be used as an theoretical data source for evaluating the performance of the signal separation between pulsatile blood flows and vessel walls with mild stenosed-lesions.


PubMed | University of Sichuan and the Second Affiliated Hospital of Kunming Medical College
Type: | Journal: American journal of otolaryngology | Year: 2015

This article has been withdrawn at the request of the author(s) and/or editor. The publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.

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