Liu K.,The Second Affiliated Hospital of Jiamusi Medical College |
Shi C.,The Second Affiliated Hospital of Jiamusi Medical College |
Liu S.-F.,The Second Affiliated Hospital of Jiamusi Medical College |
Zhang H.-K.,The Second Affiliated Hospital of Jiamusi Medical College
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Although raloxifene has been widely applied in the treatment of osteoporosis, its influence on fracture healing is unclear. OBJECTIVE: To investigate the effect of raloxifene on the healing of mandibular fractures in rabbits. METHODS: Totally 24 New Zealand white rabbits were collected. Model of bilateral mandibular angle bone defect (1.5 mm×10.0 mm) in rabbits was established. All rabbits were randomly divided into experimental and control groups. The rabbits in the experimental group were performed 7.5 mg/(kg•d) raloxifene at day 2 after modeling for 30 days, while those in the control group had no treatment. RESULTS AND CONCLUSION: X-ray observation showed that callus formation was not obvious in the two groups after modeling for 1 week. Defect area of the experimental group was indistinct, but that of the control group could be found at week 3. At week 4, callus in the experimental group was increased and medullary cavity passed again. Callus in the control group was more, and medullary cavity did not pass. Compared with control group, bone mineral density and serum bone alkaline phosphatase in the experimental group was increased obviously after modeling for 1, 3 and 4 weeks (P < 0.05), and they both reached the peak. The expression of bone morphogenetic protein 2 in callus was higher than that of the control group after modeling for 3 and 4 weeks (P < 0.01). These results suggest that raloxifene can promote osteoblast differentiation, accelerate bone mineral deposition, and induce bone morphogenetic protein 2 expression at the same time, and thereby accelerate the healing of bone fracture.