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Zhang W.,Peking Union Medical College | Chen Y.,Peking Union Medical College | Yang X.,The Second Affiliated Hospital of Baotou Medical College | Fan J.,Peking Union Medical College | And 5 more authors.
PLoS ONE | Year: 2012

Background: The development of peripheral arterial disease (PAD) is heterogeneous even in the presence of similar risk factors. Our aim was to determine whether inter-individual differences in leukocyte telomere length contribute to the susceptibility of PAD. Methods: A total of 485 patients with PAD (defined by the ankle-brachial index) and 970 age- and gender-matched controls were recruited from seven rural communities in Henan Province in China. The relative leukocyte telomere length was determined by a quantitative PCR-based method. Two common promoter variants of the hTERT gene were genotyped to assess their effects on telomere length and the risk of PAD. In vivo luciferase assay was performed to study the transcriptional activity. Results: After adjustment for vascular risk factors and genetic variants in the hTERT gene, individuals in the lowest and middle tertiles of telomere length had a significantly higher risk of PAD than did those in the highest tertile (odds ratio [OR] 1.73, 95% confidence interval [CI] 1.29-2.49 in the middle tertile; 3.15, 95%CI 2.31-4.29 in the lowest tertile). Haplotype analysis using the 2 variants (rs2735940 and rs2853669) showed that subjects with the at-risk C-C haplotype had shorter telomere length than those individuals with the T-T haplotype and consistently had 1.30-fold (OR 1.30, 95%CI 1.06-1.58; P = 0.005) increased risk for PAD. The C-C haplotype had 43% lowered transcription activity of hTERT promoter (P<0.001). Conclusion: The associations between the functional haplotype of hTERT gene and telomere length and the risk of atherosclerotic PAD suggested that mean leukocyte telomere length may independently serve as a potential predictor of PAD. © 2012 Zhang et al.


Wang C.-L.,The First Affiliated Hospital of Baotou Medical College | Liu L.-P.,The First Affiliated Hospital of Baotou Medical College | Han L.-H.,Baotou Medical College | Su Y.,Baotou Medical College | And 7 more authors.
Toxicological and Environmental Chemistry | Year: 2016

Electrophoresis release test (ERT) was established by our lab to observe the re-released hemoglobin (Hb) from red blood cells (RBCs) and whole blood. In this study, ERT was performed to study the effects of different plasma components including plasma, serum, albumin, globulin, fibrinogen, glucose, amino acid, vitamin, insulin, hormone, and inorganic ions on re-released Hb from RBC and whole blood samples during ERT. The results showed that plasma, serum, albumin, globulin, compound amino acid, essential amino acid, vitamin C, insulin, hormone, NaCl, KCl, CaCl2, and NaHCO3 − decreased re-released RBC Hb; while glucose, vitamin B1, vitamin B2, and vitamin B12 elevated re-released RBC Hb. The differing effects of various plasma components on re-released Hb of RBC may play a significant role in blood conservation. © 2016 Informa UK Limited, trading as Taylor & Francis Group


Pan X.-P.,The Second Affiliated Hospital of Baotou Medical College | Dang T.,The Second Affiliated Hospital of Baotou Medical College | Meng X.-M.,The Second Affiliated Hospital of Baotou Medical College | Xue K.-C.,The Second Affiliated Hospital of Baotou Medical College | And 2 more authors.
Cell Biochemistry and Biophysics | Year: 2011

Post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) is one of the most common and serious complications of ERCP, which has become a major concern for digestive endoscopists. In the present study, we examine whether pancreatic duct stenting can reduce the incidence of PEP. Forty patients who underwent an ERCP in our hospital were selected according to their risk factors for PEP. They were randomly divided into two groups: (1) 20 subjects received a pancreatic duct stenting after ERCP to prevent pancreatitis (stent group, S); and (2) the other 20 subjects did not receive pancreatic duct stenting after ERCP (non-stent group, NS). Urine and serum amylase, and serum lipase were determined after the operation; symptoms of abdominal pain were monitored; cost of hospitalization was evaluated for the comparison. The results show that (1) 18 cases developed PEP, and they were significantly less in S group than in NS group (4 vs. 14 cases; P < 0. 01); (2) Serum amylase was significantly lower in S group than in NS group (197. 8 ± 339. 7 vs. 825. 4 ± 1253. 4 U/l, respectively; P < 0. 05); (3) The intensity of abdominal pain was 3. 4 ± 0. 8 in S group, compared to 4. 1 ± 1. 2 in NS group; (4) Duration of pharmaceutical treatment was not significantly different between the two groups (4. 2 ± 1. 4 vs. 6. 1 ± 2. 3 days, in S and NS group, respectively); and (5) The total hospitalization cost was significantly less in S group (8928 ± 2635 RMB) than in NS group (11288 ± 4325 RMB; P < 0. 05). It is concluded that pancreatic duct stenting can reduce the incidence of PEP, shorten the duration of hospitalization, and therefore, lessen patients' financial burden. It is shown to be an effective way to prevent PEP. © 2011 Springer Science+Business Media, LLC.


PubMed | The Second Affiliated Hospital of Baotou Medical College
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2011

Post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) is one of the most common and serious complications of ERCP, which has become a major concern for digestive endoscopists. In the present study, we examine whether pancreatic duct stenting can reduce the incidence of PEP. Forty patients who underwent an ERCP in our hospital were selected according to their risk factors for PEP. They were randomly divided into two groups: (1) 20 subjects received a pancreatic duct stenting after ERCP to prevent pancreatitis (stent group, S); and (2) the other 20 subjects did not receive pancreatic duct stenting after ERCP (non-stent group, NS). Urine and serum amylase, and serum lipase were determined after the operation; symptoms of abdominal pain were monitored; cost of hospitalization was evaluated for the comparison. The results show that (1) 18 cases developed PEP, and they were significantly less in S group than in NS group (4 vs. 14 cases; P < 0.01); (2) Serum amylase was significantly lower in S group than in NS group (197.8 339.7 vs. 825.4 1253.4 U/l, respectively; P < 0.05); (3) The intensity of abdominal pain was 3.4 0.8 in S group, compared to 4.1 1.2 in NS group; (4) Duration of pharmaceutical treatment was not significantly different between the two groups (4.2 1.4 vs. 6.1 2.3 days, in S and NS group, respectively); and (5) The total hospitalization cost was significantly less in S group (8928 2635 RMB) than in NS group (11288 4325 RMB; P < 0.05). It is concluded that pancreatic duct stenting can reduce the incidence of PEP, shorten the duration of hospitalization, and therefore, lessen patients financial burden. It is shown to be an effective way to prevent PEP.

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