The Samuel Robert Noble Foundation
The Samuel Robert Noble Foundation
Kanchupati P.,South Dakota State University |
Wang Y.,South Dakota State University |
Wang Y.,Northwest Agriculture and Forestry University |
Anower M.R.,South Dakota State University |
And 4 more authors.
Crop Science | Year: 2017
We recently identified the alfalfa (Medicago sativa L.) germplasm River Side (RS) and Foster Ranch (FR), both naturally adapted to the Grand River National Grassland environment in South Dakota and showing superior freezing tolerance. To understand the molecular basis of freezing tolerance in RS and FR, we examined expression of the C-repeat binding factor-like (CBFl) genes in alfalfa. Eighteen CBFl genes were identified after examining the genome of Medicago truncatula Gaertn., a close relative of alfalfa. Phylogenetic analysis clustered Medicago CBFs into four subgroups. Expression profiling of these genes in alfalfa seedlings revealed diverse cold-induction patterns. Four of the genes that showed an early induction as CBF3 in Arabidopsis under cold stress were selected for detailed expression analyses. These genes varied in expression patterns, in different tissues and at different developmental stages, and exhibited different diurnal regulation without cold treatment. Two of the genes, MsCBFl-17 and MsCBFl-18, showed an early and high induction under cold stress in RS and Apica, a cold-tolerant cultivar, when compared with a nonfreezing tolerant germplasm, suggesting that these two genes are potentially the functional homologs of CBF3. On the other hand, MsCBFl-11 was the only gene that was induced in all three cold-tolerant germplasm, including FR, but the induction was relatively late compared with MsCBFl-17 and MsCBFl-18. Together, these findings suggest that the CBFs may play an important role in the regulation of freezing tolerance in alfalfa, and additional mechanisms exist to explain the superior freezing tolerance in RS and FR. © Crop Science Society of America | 5585 Guilford Rd., Madison, WI 53711 USA. All rights reserved.
PubMed | The Samuel Robert Noble Foundation
Type: Journal Article | Journal: Molecular plant-microbe interactions : MPMI | Year: 2012
Cucumber mosaic virus (CMV) associated with D satellite RNA (satRNA) causes lethal systemic necrosis (LSN) in tomato (Solanum lycopersicum), which involves programmed cell death. No resistance to this disease has been found in tomato. We obtained a line of wild tomato, S. habrochaitis, with a homogeneous non-lethal response (NLR) to the infection. This line of S. habrochaitis was crossed with tomato to generate F1 plants that survived the infection with NLR, indicating that NLR is a dominant trait. The NLR trait was successfully passed on to the next generation. The phenotype and genotype segregation was analyzed in the first backcross population. The analyses indicate that the NLR trait is determined by quantitative trait loci (QTL). Major QTL associated with the NLR trait were mapped to chromosomes 5 and 12. Results from Northern blot and in situ hybridization analyses revealed that the F1 and S. habrochaitis plants accumulated minus-strand satRNA more slowly than tomato, and fewer vascular cells were infected. In addition, D satRNA-induced LSN in tomato is correlated with higher accumulation of the minus-strand satRNA compared with the accumulation of the minus strand of a non-necrogenic mutant D satRNA.
Morsy M.R.,The Samuel Robert Noble Foundation |
Oswald J.,The Samuel Robert Noble Foundation |
He J.,The Samuel Robert Noble Foundation |
Tang Y.,The Samuel Robert Noble Foundation |
Roossinck M.J.,The Samuel Robert Noble Foundation
Biochemical and Biophysical Research Communications | Year: 2010
The fungus Curvularia protuberata carries a dsRNA virus, Curvularia thermal tolerance virus, and develops a three-way symbiotic relationship with plants to enable their survival in extreme soil temperatures. To learn about the genome of C. protuberata and possible mechanisms of heat tolerance a collection of expressed sequence tags (ESTs) were developed from two subtracted cDNA libraries from mycelial cultures grown under control and heat stress conditions. We analyzed 4207 ESTs that were assembled into 1926 unique transcripts. Of the unique transcripts, 1347 (70%) had sequence similarity with GenBank entries using BLASTX while the rest represented unknown proteins with no matches in the databases. The majority of ESTs with known similarities were homologues to fungal genes. The EST collection presents a rich source of heat stress and viral induced genes of a fungal endophyte that is involved in a symbiotic relationship with plants. Expression profile analyses of some candidate genes suggest possible involvement of osmoprotectants such as trehalose, glycine betaine, and taurine in the heat stress response. The fungal pigment melanin, and heat shock proteins also may be involved in the thermotolerance of C. protuberata in culture. The results assist in understanding the molecular basis of thermotolerance of the three-way symbiosis. Further studies will confirm or refute the involvement of these pathways in stress tolerance. © 2010 Elsevier Inc.