The PLA 117 Hospital

Hangzhou, China

The PLA 117 Hospital

Hangzhou, China
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Hu H.,Zhejiang Chinese Medical University | Hu H.,Nanchang University | Hu H.,Zhejiang University of Technology | Zhu Q.,Wenzhou University | And 10 more authors.
Medical Science Monitor | Year: 2017

Background: Paeoniflorin is a monoterpene glycoside extracted from the roots of Paeonia lactiflora and is used in Chinese herbal medicine to treat hyperlipidemia. The aim of this study was to evaluate the effects of an enriched extract of paeoniflorin on cholesterol levels, hemodynamics, and oxidative stress in a hyperlipidemic rat model. Material/Methods: Male Sprague-Dawley rats were fed high-cholesterol diets and treated with three different doses of paeoniflorin for 12 weeks. The effects of paeoniflorin treatment were assessed on cholesterol levels, cholesterol metabolism, red blood cell vascular flow using hemorheology, antioxidant enzymes, and expression of the rate-limiting enzyme in the mevalonate pathway, 3-hydroxy-3-methylglutharyl-coenzyme A reductase (HMG-CoAR). Rat liver histology and immunohistochemical analysis were performed to evaluate the expression of nuclear factor erythroid 2–related factor 2 (Nrf2), cytochrome P450 7A1 (CYP7A1), and peroxisome proliferator-activated receptors (PPAR)-a. Protein expression HMG-CoAR, low-density lipoprotein receptor (LDLR), PPAR-a and CYP7A1 was measured by Western blotting. Antioxidant activity in rat liver was determined by measuring superoxide dismutase (SOD) and malondialdehyde (MDA). Results: Serum and hepatic cholesterol, hepatic steatosis and the products of cholesterol metabolism were reduced by paeoniflorin treatment, which also reduced the activity of HMG-CoAR and upregulated the expression of LDLR, PPAR-a, and CYP7A1 expression, increased SOD, decreased MDA, and upregulated Nrf2 expression. Conclusions: The findings of this study in a rat model of hyperlipidemia have shown that paeoniflorin regulates hepatic cholesterol synthesis and metabolism and may also protect the liver from oxidative stress. © Med Sci Monit.

Liu M.,The PLA 117 Hospital | Zhang W.-D.,The PLA 117 Hospital | Zhang M.-O.,Hangzhou Traditional Chinese Medicine Hospital | Chen X.-C.,The PLA 117 Hospital | Zou Q.,The PLA 117 Hospital
Journal of Biomaterials and Tissue Engineering | Year: 2015

The aim of the present study was to develop sustained release (SR) matrix tablet of ketorolac tromethamine using natural polymer xanthan gum with cellulose derivative (sodiumcarboxy methyl cellulose) and Polyvinylpyrrolidone K30 for effective management of pain and inflammation in spinal injuries. The formulated tablets were evaluated for uniformity of weight, drug content, friability, hardness, thickness, swelling index, in vitro drug release and in vivo analgesic and antiinflammatory activity. The physicochemical properties of tablets were found within the limits. The SR matrix tablets of ketorolac showed good swelling (increased with xanthan gum’s concentration), drug content and the dissolution profile. The drug released increased with decrease in swelling properties. The formulation with 20% w/v of xanthan showed the lowest % drug release (49.6%) at the end of 8 hrs. It was concluded that increasing the concentration of gum from 5% w/v to 20% w/v in the formulation decreased the amount of drug release from the tablet. The formulation with 10% xanthan gum found to be the best in % pain reduction (54.6%) and % inhibition of paw edema (51.58%). The xanthan gum based SR matrix tablets of ketorolac tromethamine can be explored for potential therapeutic uses in spinal injuries. © 2015 American Scientific Publishers. All rights reserved.

Zheng J.,The PLA 117 Hospital | Yang Y.-H.,The PLA 117 Hospital | Lou S.-L.,The PLA 117 Hospital | Zhang D.-S.,The PLA 117 Hospital | Liao S.-H.,Central South University
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Spine is relatively complex in structure, shape, material properties, and load bearing. The traditional biomechanical method cannot solve these problems. Objective: To investigate the stress distribution of intervertebral disc, zygapophysial joints and vertebral body of degenerative scoliosis, and to provide accordance to the biomechanical mechanism of degenerative scoliosis occurrence and development. Methods: Based on the successive CT images of spinal column from T12 to superior segment of S1 of degenerative scoliosis patients, the special material properties were attributed to the model to form the integrated and effective three-dimensional finite element model of degenerative scoliosis. The model was loaded on the anteflexion, extension, left lateral bending, right lateral bending, left rotation and right rotation conditons. Then the spinal activity and the stress distribution of intervertebral disc, vertebral body and articular cartilage of zygapophysial joints were calculated and analyzed. Results and conclusion: The spinal activities of degenerative scoliosis finite element model were less than those of common lumbar spine. The stress distribution of intervertebral disc was inclined to the verge of it and the greatest stress was appeared on the extension motion. The apex of scoliosis was the place of stress concentration and the obvious stress concentration of articular cartilage of zygapophysial joints was appeared on the rotation motion, then followed by the extension motion, especially that of articular cartilage of zygapophysial joints on the apex of scoliosis. Stress concentration is easily appeared on the apex of scoliosis, and the extension and rotation motion can aggravate the development of degenerative scoliosis.

Chen X.-C.,The PLA 117 Hospital | Pan Y.-Q.,The PLA 117 Hospital | Zhao K.-Y.,The PLA 117 Hospital | Liu M.,The PLA 117 Hospital | He D.,The PLA 117 Hospital
Chinese Journal of Interventional Imaging and Therapy | Year: 2013

Objective: To explore the diagnostic value of hiding degenerative changes of lumbar spine with with self-controlled axial loading device of the lumbar spine. Methods: Totally 120 patients were examined by paired posas relaxed position CT/MR (PRP CT/MR) and axial compression in extension CT/MR (ACE CT/MR) with self-controlled axial loading device of the lumbar spine controlled, with compression less than 50% of the patient's weight. The results were compared with conventional CR/MR. Decrease of dural sac cross sectional area 015 mm2) to values smaller than 75 mm2, narrow lateral recess, narrow intervertebral foramen lumbar disk heriation were considered as additional valuable information (AVI). Results: AVI was found in 43 of 120 patients, including decrease of dural sac cross sectional area in 33 patients, narrow lateral recess and (or) narrow intervertebral foramen in 12, lumbar disk heriation in 11 and to a greater extent in 15 patients. AVI was found in 40 of 98 (40/98, 40.82%) patients with sciatica and 3 of 22 (3/22, 13.64%) patients with low back pain (P<0.05). Conclusion: ACE CT/MRI with self-controlled axial loading device has important value of clinical evaluation on hiding degenerative changes of lumbar spine.

Yang Y.-H.,The PLA 117 Hospital | Zheng J.,The PLA 117 Hospital | Lou S.-L.,The PLA 117 Hospital
International Journal of Clinical and Experimental Medicine | Year: 2014

Objective: To investigate the causes and managements of early postoperative complications of degenerative scoliosis (DS) treated with internal pedicle screw fixation. Methods: From Jan 2000 to Apr 2013, 325 DS patients treated with internal pedicle screw fixation in our hospital were retrospectively involved. The categories, causes, managements and outcomes of early postoperative complications were statistically analyzed. Results: Early postoperative complications occurred in 10.76% of the patients including 16 cases of lower limb numb or pain, 6 cases of decreased lower limb sensitivity and motor functions, which accounted for 62.86% of all complications, followed by incision infections (4/35, 11.43%) and rare cases of cerebrospinal fluid leakage, cardiac and renal inadequacy, urinary system and pulmonary infections. The incidence of overall complications (19.79%, p = 0.001) and nerve injuries (11.46%, p = 0.000) were significantly higher in long-segment than in short-segment fixations. Improper screw implanting, over correction of scoliosis and insufficient blood supply of the spinal cord during operation were risk factors for early postoperative complications and most of them were cured by anti-infection medication, incision dressing change, nerve nourishment, adjusting the screws and anti-osteoporosis treatments within 6 months after surgery. Only three cases with severe nerve injury did not improve until the 6 months postoperative follow-up. Conclusions: Most of the postoperative complications in our DS patients disappeared within 6 months after surgery and more than half of complications were nerve injuries. © 2014, E-Century Publishing Corporation. All Rights Reserved.

Hu J.-B.,Zhejiang University | Dong M.-J.,Ningbo No2 Hospital | Dong M.-J.,The PLA 117 Hospital | Zhang J.,The PLA 117 Hospital
Tropical Journal of Pharmaceutical Research | Year: 2016

Purpose: To design a dual inhibitor of natural origin capable of targeting ErbB1 and ErbB2 kinases for the treatment of lung cancer. Method: Advanced In silico drug designing techniques were explored in this study. Sequence and structure analysis of ErbB1 and ErbB2 was followed by three dimensional (3D) pharmacophore. The generated model was used for molecular docking simulation studies for predicting the best natural dual inhibitors, the selected inhibitors were subjected to absorption, distribution, metabolism, excretion and toxicology (ADME/Tox) prediction. Results: The results confirmedfive phytochemicals, viz. hyoscyamide, cannabisin F, cochinchinenene D, cannabisin E, and heliotropamide and five FDA approved drugs namely fesoterodine, antrafenine, fluspirilene, posaconazole, and iloprost to be potential inhibitors of both ErbB1 and ErbB2. The shortlisted compounds from both the panels were showing better MolDock score than the two reference drugs (Lapatinib and Afatinib). Conclusion: The 3D pharmacophore modelling and molecular docking simulations gave us ten compounds that successfully exploited dual inhibition of ErbB1 and ErbB2. With 8 and 12 hydrogen bonds with ErbB1 and ErbB2 respectively cannabisin F showed best interaction of all. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.

PubMed | The PLA 117 Hospital
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2013

The aim of this study was to detect MTA2 expression in pancreatic ductal adenocarcinoma (PDA) and to analyze its association with prognosis of PDA patients. MTA2 mRNA and protein expression were determined by real-time quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry in specimens of primary cancer and their adjacent noncancerous tissues in PDA patients. We found that MTA2 mRNA and protein expression levels were both significantly upregulated in PDA lesions compared with adjacent noncancerous tissues. Immunohistochemistry showed that high MTA2 expression was correlated with poor tumor differentiation, TNM stage, and lymph node metastasis. Kaplan-Meier survival analysis showed that patients with high expression levels of MTA2 showed lower overall survival rate than those with low expression levels. Multivariate analysis showed that high MTA2 protein expression was an independent prognostic factor for PDA patients. Our study suggests that overexpression of MTA2 may play an important role in the progression of PDA and MTA2 expression may serve as a biomarker for poor prognosis for PDA.

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