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Manila, Philippines

Masangkay F.R.,Institute of Tropical Medicine | Masangkay F.R.,The Philippine Womens University | Masangkay F.R.,Far Eastern University of Manila
International Journal of Mycobacteriology

Objective: To investigate the performance of " tofu-whey liquid medium" for the propagation of . Mycobacterium tuberculosis (MTB) strain H37Rv. Method: Two hundred micro liters (200. μl) of 1 McFarland standard (1. mg/ml-bacillary suspension) were inoculated into different batches of tofu-whey liquid medium. Each series contained three trials of test (tofu-whey liquid medium) and control media (Middlebrook 7H9 medium). Turbidity was measured within three weeks of inoculation using a nephelometer. The combinations of various tofu-whey liquid culture media were as follows; T1 (tofu-whey. +. ADC. +. glycerol. +. Potassium sulfate. +. Magnesium citrate. +. Sodium glutamate); T2 (tofu-whey. +. ADC. +. glycerol. +. Potassium sulfate. +. Magnesium citrate); T3 (tofu-whey. +. ADC. +. glycerol. +. Potassium sulfate); T4 (tofu-whey. +. ADC. +. glycerol); T5 (tofu-whey. +. ADC); and T6 (tofu-whey only). Results: In all test and control liquid culture media, the multiplication of . M. tuberculosis was documented under light and fluorescence microscopy. Of various tofu-whey medium used, T1 demonstrated the most potential for MTB propagation. The increased turbidity reading represented by the value in " unit drop of % transmittance" was higher (25 scores) in the T1 tofu-whey medium, compared with the T6 tofu-whey medium (8 scores). The overall growth was significantly better in Middlebrook 7H9 culture media, although by the third day of incubation, the bacillary growth was superior in the T1 tofu-whey culture media. Sub-cultures in Lowenstein-Jensen (L-J) medium yielded between 87% (47 of 54) and 89% (48 of 54) recovery rate with between 7% and 13% contamination rate with coagulase-negative staphylococci. Conclusion: Tofu-whey media can be used as an economical alternative to Middlebrook 7H9 in resource-limited settings. © 2012 Asian-African Society for Mycobacteriology. Source

Hilario J.E.,University of the Philippines at Diliman | Hilario J.E.,The Philippine Womens University | Perez T.R.,Ateneo de Manila University
Philippine Agricultural Scientist

Inflows from the Balete, Wawa and Laurel Rivers and from fish cages within Taal Lake, Batangas, Philippines were studied to assess the input of nitrogen (N) and phosphorus (P) into the lake. Physical parameters such as temperature, total dissolved solids (TDS), pH, conductivity and dissolved oxygen (DO) were recorded in situ. Nutrient concentration and wind data collected were used as inputs in a modified nutrient transport model simulating advection and dispersion of nutrient concentrations in Taal Lake. Results showed significant concentrations of P (P<0.01) and N (P<0.05) in Laurel River, with mean discharges of 5 and 4 kg d-1, respectively, while Balete and Wawa Rivers contributed high mean discharges of 85 kg d-1 nitrate-nitrogen (NO3-N). Measurements of TDS and conductivity were similarly significant (P<0.01) in the fish cages. Simulated distribution patterns of nutrients revealed that during the Northeast (NE) monsoon, nutrient discharges from the Laurel and Balete Rivers were transported southward while nutrients coming from the Wawa River moved in the northwest and southwest directions. During the Southwest (SW) monsoon, nutrients from Laurel were transported northwest while nutrients from Wawa and Balete moved in the southwest direction. High N and P concentrations in Laurel could be attributed to the neutral pH measured throughout the study period since P is soluble and N-fixers grow best at circum-neutral pH. NH4-N and NO3-N are soluble ions and are easily leached, so that losses of these ions from soils to freshwaters reflect the amount of fixation in the soils. A nutrient transport model of Taal Lake was formulated to quantitatively analyze the transport and fate of P and N nutrients that enter the lake via tributaries. Nutrient discharges into the lake were transported by water movements which had three main influences: height differences of surface level with gravitational flow, density with buoyancy or sinking and surface wind stress with transfer of momentum as well as energy. Wind stress was responsible for the slow nutrient transport in the lake. Source

The Philippine Womens University | Entity website

The HZB-SIRDs Bachelor and Master of Arts in Foreign Services curricula are primarily designed to help students and professionals achieve their goals: to start a career in diplomacy and international relations or to enhance further the practitioners knowledge about the subjects. BACHELOR OF ARTS IN FOREIGN SERVICE ...

The Philippine Womens University | Entity website

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Cervo M.M.C.,The Philippine Womens University | Llido L.O.,The Philippine Womens University | Llido L.O.,Rush University Medical Center | Barrios E.B.,University of the Philippines at Diliman | Panlasigui L.N.,The Philippine Womens University
Journal of Nutrition and Metabolism

This randomized, controlled trial examined the effects of canned pineapple consumption on immunomodulation, nutritional status, and physical health of ninety-eight (98) school children with mean age of 8.44±0.20. The study participants were divided into three groups: Group A (33) includes subjects who were not given canned pineapple, Group B (33) includes those who were given 140 g, and Group C (32) includes those given 280 g of canned pineapple for nine weeks. Each major group was further divided into two groups: normal (N) and underweight (U) based on 2007 WHO Growth Reference Standards. Sociodemographic, anthropometric, physical examination, dietary intake, hemoglobin level, and immunological data were analyzed. Results showed a decrease in incidence of viral and bacterial infections for both Group B and Group C (normal and underweight) after canned pineapple consumption. Granulocyte production increased by 0.77-26.61% for normal weight subjects and 14.95-34.55% for underweight. CD16+56 count augmented by 20.44-22.13% for normal weight and 3.57-15.89% for underweight subjects. Thus, intake of both one can (140 g) and two cans (280 g) of canned pineapple may shorten the duration and incidence of infection and may increase the production of granulocytes and CD16+56, but intake of two cans (280 g) demonstrated higher granulocyte and CD16+56 production. This trial is registered with Philippine Health Research Registry: PHRR140826-000225. © 2014 Mavil May C. Cervo et al. Source

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