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Cui X.-G.,Shandong University | Liu C.-Y.,The Peoples Hospital of Zhangqiu | Wei B.,General Hospital of Yanzhou Coal Mining company | Zhao W.-J.,General Hospital of Yanzhou Coal Mining company | Zhang W.-F.,General Hospital of Yanzhou Coal Mining company
Mitochondrial DNA | Year: 2014

Animal models played an important role in osteoarthritis studies. Here, the complete mitochondrial genome sequence of the Guinea pig was reported for the first time. The total length of the mitogenome was 16,797 bp. It contained the typical structure, including two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and one non-coding control region (D-loop region). The overall composition of the mitogenome was estimated to be 34.9% for A, 26.1% for T, 26.0% for C and 13.0% for G showing an A-T (61.0%)-rich feature. This mitochondrial genome sequence will provide new genetic resource into osteoarthritis disease. © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted Source

Zhang K.,Qingdao University | Liu X.,The Peoples Hospital of Zhangqiu | Hao F.,Qingdao University | Dong A.,Qingdao University | Chen D.,Qingdao University
American Journal of Translational Research | Year: 2016

Objective: Anaplastic thyroid cancer (ATC) is one of the most lethal human malignancies. However, the molecular mechanisms of ATC invasion are poorly understood. The transforming growth factor-beta (TGF-β) signaling pathway plays a critical role in promoting tumor metastasis. TGF-β1 was found to be overexpressed in anaplastic thyroid cancer (ATC). We therefore tested our hypothesis that targeted down-regulation of TGF-β1 inhibits invasion of ATC cells. Methods: Effects of TGF-β1 stimulation or TGF-β1 sliencing by small interfering RNA (TGF-β1 siRNA) on invasion in 8505C and SW1736 cells in vitro was detected. Using siRNAs and inhibitors to examine the TGF-β1 signaling pathway. Results: TGF-β1 siRNA inhibits cell migration and invasion in vitro, followed by inactivation of pSMAD2, S100A4 and MMP-2/9. TGF-β stimulation activated pSMAD2-dependent S100A4 and MMP-2/9 expression, and increased cell migration and invasion. The depletion of pSMAD2 or S100A4 or MMP-2/9 expression inhibited TGF-β signaling pathway. Moreover, it significantly weakened the proinvasive effects of TGF-β on ATC cells. Conclusions: Therapies targeting the TGF-β1 inhibits invasion of ATC cells by impeding the SMAD2-dependent S100A4-MMP-2/9 signalling in vitro. © 2016, E-Century Publishing Corporation. All rights reserved. Source

Qian T.,Fudan University | Chen M.,Fudan University | Gao F.,Fudan University | Meng F.,Fudan University | And 2 more authors.
Magnetic Resonance Imaging | Year: 2014

Objective: To evaluate the correlation between findings from diffusion weighted imaging (DWI) and microvascular density (MVD) measurements in VX2 liver tumors after transarterial embolization ablation (TEA). Materials and Methods: Eighteen New Zealand white rabbits were used in this study. VX2 tumor cells were implanted in livers by percutaneous puncture under computed tomography (CT) guidance. Two weeks later, all rabbits underwent conventional magnetic resonance imaging (MRI) (T1 and T2 imaging), DWI, (b=100, 600, and 1000s/mm2) and TEA. MRI was performed again1 week after TEA. Liver tissue was then harvested and processed for hematoxylin and eosin (H&E) staining and immunohistochemical staining for CD31to determine MVD. Results: VX2 liver tumors were successfully established in all 18 rabbits. Optimal contrast was achieved with a b value of 600s/mm2.The maximum pre-operative apparent diffusion coefficient (ADC)difference value was 0.28×10-3±0.10×10-3mm2/s, and was significantly different (P<0.001) from the maximum postoperative ADCdifference value of 0.47×10-3±0.10×10-3mm2/s. However, the mean ADC value for the entire tumor was not significantly correlated with MVD (r=0.221, P=0.379), nor was the ADC value for the regions of viable tumor (r=-0.044, P=0.862). However, the maximum postoperative ADCdifference value was positively correlated with MVD(r=0.606, F=12.247, P=0.003). © 2014 Elsevier Inc. Source

Liu J.,Central South University | Liu J.,Tsinghua University | Li N.,The Peoples Hospital of Zhangqiu | Li L.,Central South University | And 8 more authors.
Oncology Letters | Year: 2013

Magnetic-mediated hyperthermia (MMH) is a promising local thermotherapy approach for cancer treatment. The present study investigated the feasibility and effectiveness of MMH in esophageal cancer using a rabbit tumor model. The therapeutic effect of two hyperthermia approaches, magnetic stent hyperthermia (MSH), in which heat is induced by the clinical stent that is placed inside the esophagus, and magnetic fluid hyperthermia (MFH), where magnetic nanoparticles are applied as the agent, was systematically evaluated. A rabbit esophageal tumor model was established by injecting VX2 carcinoma cells into the esophageal submucosa. The esophageal stent was deployed perorally into the tumor segment of the esophagus. For the MFH, magnetic nanoparticles (MNPs) were administered to the rabbits by intratumoral injection. The rabbits were exposed under a benchtop applicator using an alternative magnetic field (AMF) with 300 kHz frequency for the hyperthermia treatment. The results demonstrated that esophageal stents and MNPs had ideal inductive heating properties upon exposure under an AMF of 300 kHz. MSH, using a thermal dose of 46°C with a 10-min treatment time, demonstrated antitumor effects on the rabbit esophageal cancer. However, the rabbit esophageal wall is not heat-resistant. Therefore, a higher temperature or longer treatment time may lead to necrosis of the rabbit esophagus. MFH has a significant antitumor effect by confining the heat within the tumor site without damaging the adjacent normal tissues. The present study indicates that the two hyperthermia procedures have therapeutic effects on esophageal cancer, and that MFH may be more specific than MSH in terms of temperature control during the treatment. Source

Li G.,Shandong University | Wang N.,The Peoples Hospital of Zhangqiu | Sun C.,The Peoples Hospital of Zhangqiu | Li B.,The Peoples Hospital of Zhangqiu
World Journal of Surgical Oncology | Year: 2014

Background: It has been demonstrated that eIF3f expression is significantly decreased in many human cancers, a fact which plays an important role in human cancer. However, the expression of eIF3f in gastric cancer (GC) is not well understood to date. Therefore, the aim of this study is to detect the expression of eIF3f in GC. Methods: The expression of eIF3f was examined by immunohistochemistry in tissues with stage I to III GC and adjacent non-cancerous tissues (ANCT) of 195 gastrectomy specimens; clinicopathological results, including survival, were analyzed. Results: The positive expression rate of eIF3f was significantly higher in ANCT tissues than in GC. eIF3f levels were correlated with more advanced tumor stages and likelihood of recurrence (all P <0.05). The Kaplan-Meier survival curves indicated that decreased expression of eIF3f could serve as a prognosis marker for poor outcome of GC patients (P = 0.04). Conclusions: eIF3f may play an important role in recurrence, thus representing a promising predictive marker for the prognosis of GC. © 2014 Li et al.; licensee BioMed Central Ltd. Source

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