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Cui C.,Sun Yat Sen University | Zhang M.,The Peoples Hospital of Laiwu | Tian L.,Sun Yat Sen University | Jiang W.,Sun Yat Sen University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Purpose:To determine the correlation between pretreatment computed tomography (CT) data and survival duration after neoadjuvant chemoradiotherapy and surgery for locally advanced rectal cancer. Materials and meth­ods: 122 consecutive patients with advanced rectal cancer were assessed retrospectively. Pretreatment imaging and postoperative data were evaluated through Kaplan-Meier and Cox proportional hazard regression analyses. Results: Pretreatment CT identified 557 metastatic lymph nodes (mean, 4.55 per patient; median 4). Survival dura­tions were measured during the period between the application of CT and death or the last follow-up examination. Univariate analysis showed that the following factors had a significant impact on survival: maximum tumor diameter (P = 0.019), distance from inferior tumor margin to anorectal ring (P <0.0001), number of lymph nodes involved in patients with short-axis, lymph node diameter ≥8 mm (P <0.0001) in pretreatment CT, distance from the anorectal ring (P = 0.027), ypN stage (P = 0.0008), ypM stage (P = 0.046) and number of metastatic lymph nodes (P <0.0001) in clinical assessment. Multivariate analysis showed that the following factors were significant: number of lymph nodes in patients with short-axis lymph node diameter ≥5 mm but <8 mm (P = 0.044) and in those with this diam­eter ≥8 mm (P = 0.028; pretreatment CT) and number of metastatic lymph nodes (assessed in histopathological examination). Conclusion: Pretreatment lymph node size and number can predict survival duration after treatment for locally advanced rectal cancer. For patients with lymph nodes >8 mm (short-axis diameter) and/or >1, such lymph nodes tend to have a poor performance for prognosis. © 2015, E-Century Publishing Corporation. All rights reserved.

Wang D.,Shandong University | Jiang H.,Shandong University | Wang S.,The Peoples Hospital of Laiwu | Li H.,Shandong University | And 5 more authors.
Experimental and Therapeutic Medicine | Year: 2014

The aim of the present study was to construct tissue-engineered bone using a bioreactor and platelet-rich plasma (PRP). Bone marrow mesenchymal stem cells (BMSCs) and β-tricalcium phosphate (β-TCP) were cultured in a perfusion bioreactor with PRP-containing medium for 21 days to form a BMSC-TCP composite. Rabbits were then implanted with the BMSC-TCP composite. The morphology of the implanted BMSC-TCP composite was observed three months after surgery by scanning electron microscopy and hematoxylin and eosin (H&E) staining. In addition, the expression of cluster of differentiation (CD)31 and von Willebrand factor (WF) in the implanted BMSC-TCP composite was detected using immunohistochemistry. Bone formation was determined by comprehensive testing Following culture in a perfusion bioreactor and PRP, the BMSCs adhered to the β-TCP scaffold and the secretion of extracellular matrix was observed. The spreading and proliferation of cells was found to be enhanced on the scaffold. Furthermore, the vascular endothelial cell markers CD31 and VEF, were positively expressed. Therefore, these results suggest that tissue-engineered bone may be constructed using a bioreactor and PRP. PRP, which contains multiple growth factors, may promote vascularization of tissue-engineered bone.

Deng Y.,The Peoples Hospital of Laiwu | Geng L.,The Peoples Hospital of Laiwu
Journal of Practical Oncology | Year: 2012

Objective: To investigate the expression and clinical significance of Matriptase in cervical carcinoma. Methods: Immunohistochemistry method was used to detect expression of Matriptase in 70 cervical carcinoma tissues, 30 cervical intraepithelial neoplasia(CIN) tissues and 20 nomal cervical epithelia tissues. The relationship between positive rate and pathological type, clinical pathological features was analyzed. Results: Positive rate of Matriptase in cervical intraepithelial neoplasia(CIN) and cervical carcinoma tissues was 56.7% and 65.7%, which was higher than that in normal tissues, showing significant difference(P<0.05). In cervical carcinoma, expression of Matriptase protein was significantly correlated with different histological classifications, FIGO staye, and lymph nede metastasis(P<0.05). But there was no relation with pathological types. Conclusion: Matriptase may play an important role in the occurrence, development, invasion and metastasis of cervical intraepithelial neoplasia(CIN) and cervical carcinoma, it might be a potential biomarker for the diagnosis of cervical intraepithelial neoplasia(CIN) and cervical carcinoma.

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