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Ye X.-W.,State Key Laboratory of Biotherapy of China | Ye X.-W.,University of Sichuan | Xiao M.,State Key Laboratory of Biotherapy of China | Xiao M.,University of Sichuan | And 8 more authors.
International Journal of Immunogenetics | Year: 2011

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease characterized by airway obstruction that is not fully reversible, and there is evidence of a hereditary component in COPD. We aimed to determine whether the polymorphisms -2548G/A of leptin (LEP) gene were associated with COPD and its severity in Chinese. A total of 456 subjects with COPD and 422 healthy controls from West China Hospital were enrolled in this study. COPD patients had been undergone a spirometry and a physical examination to refer the GOLD I-IV stages. The polymorphisms in the leptin promoter region at position -2548 G/A were detected by Polymerase chain reaction-restriction fragment length polymorphism analysis. The genotypes and alleles were scored, and the frequencies of the alleles and genotypes in patients and controls were compared. A significantly higher risk for COPD was observed for carriers of the LEP -2548 AA genotype [odds ratio (OR) = 7.87, 95% confidence interval (CI) 4.19-14.77, P < 0.001] and carriers of the LEP -2548 GA genotype (OR = 2.98, 95% CI 1.57-5.66, P = 0.001). The LEP -2548 A allele: frequency was significantly higher in the patient group compared with the control group (OR = 2.75, 95% CI: 2.20-3.44, P < 0.001). We also found a significant relationship between leptin gene polymorphism and the severity of COPD. In the present case-control study, we found an association between the -2548 G/A variant of the leptin gene and pathogenesis, severity of COPD in the Chinese population. It suggests that leptin -2548 G/A should be used as a genetic marker of COPD severity. © 2010 Blackwell Publishing Ltd.

Hu X.,The Peoples Hospital of Guizhou Province
Chinese Journal of Neurology | Year: 2011

Objective: To study the regulation effect of estrogen in expression of matrix metalloproteinase-9 (MMP-9) in the central nervous system (CNS) in mice with experimental autoimmune encephalomyelitis (EAE). Methods: The 60 mice were overiectomized and 2 weeks later EAE was induced with MOG 35-55 peptide in these mice. They were divided into a treatment group and a control group. The treatment group was treated with estrogen and the control group was given PBS. Clinical symptoms in these two groups were scored and compared. HE staining was used to observe inflammation in the brain and spinal cord. The MMP-9 expression in the CNS was examined by quantitative real-time PCR and immunofluorescence staining. Results: The incidence of disease was lower (treatment and control group were 8/30 and 28/30 respectively) and clinical symptoms were milder (treatment and control group were 3.23±0.83 and 1.62±1.00 respectively, t=3.811 and P<0.05) in the treatment group than those in the control group. HE staining showed the decreased infiltration of inflammatory cell in the treatment group (Treatment group: inflammatory score were 0.895±0.206, 0.752±0.302, 0.732±0.183 in acute, relief and chronic phase respectively; Control group: inflammatory score were 3.472±0.635, 2.881±0.662, 1.891±0.482 in acute, relief and chronic phase respectively, t=8.622, 6.543 and 5.027, all P<0.05). The quantitative real-time PCR and immunofluorescence staining showed that the expression of MMP-9 in the CNS was decreased in the treatment group. Conclusion: Estrogen may decrease MMP-9 expression in the CNS, reduce inflammation and clinical symptoms in mice with EAE.

Wang L.,The Peoples Hospital of Guizhou Province | Guo P.,The Peoples Hospital of Guizhou Province
International Journal of Clinical and Experimental Medicine | Year: 2016

Leukemia has severely endangered people healthy. Imatinib mesylate is a newly-developed targeting drug for treating leukemia. This study aimed to investigate the molecular mechanism of imatinib mesylate on regulating K562 cell apoptosis via mediating micro RNA-139 (miR-139) expression. K562 cells were treated with imatinib mesylate and were examined for their growth, proliferation and apoptosis by flow cytometry. MiR-139 expression level was determined by RT-PCR. We also synthesized miR-139 expressing vectors and transfected them into K562 cells, followed by imatinib mesylate treatment and cell viability and apoptotic assays. The inhibition of K562 cell growth and proliferation occurred after imatinib mesylate treatment, accompanied with cell apoptosis. In leukemia patient sample, the expression level of miR-139 was significantly higher than normal people. K562 cell over-expressing miR-139 had depressed level of imatinib mesylate-induced cell apoptosis. Imatinib mesylate induces the apoptosis of K562 cells via suppressing miR-139 expression. © 2016, E-Century Publishing Corporation. All rights reserved.

Wang Y.-J.,Southern Medical University | Wang Y.-J.,Pearl Laboratory Animal Science and Technology Co. | Liu W.,Southern Medical University | Chen C.,The Peoples Hospital of Guizhou Province | And 5 more authors.
PLoS ONE | Year: 2013

Background: The radiation-induced energy metabolism dysfunction related to injury and radiation doses is largely elusive. The purpose of this study is to investigate the early response of energy metabolism in small intestinal tissue and its correlation with pathologic lesion after total body X-ray irradiation (TBI) in Tibet minipigs. Methods and Results: 30 Tibet minipigs were assigned into 6 groups including 5 experimental groups and one control group with 6 animals each group. The minipigs in these experimental groups were subjected to a TBI of 2, 5, 8, 11, and 14 Gy, respectively. Small intestine tissues were collected at 24 h following X-ray exposure and analyzed by histology and high performance liquid chromatography (HPLC). DNA contents in this tissue were also examined. Irradiation causes pathologic lesions and mitochondrial abnormalities. The Deoxyribonucleic acid (DNA) content-corrected and uncorrected adenosine-triphosphate (ATP) and total adenine nucleotides (TAN) were significantly reduced in a dose-dependent manner by 2-8 Gy exposure, and no further reduction was observed over 8 Gy. Conclusion: TBI induced injury is highly dependent on the irradiation dosage in small intestine and inversely correlates with the energy metabolism, with its reduction potentially indicating the severity of injury. © 2013 Wang et al.

Zhou T.,The Peoples Hospital of Guizhou Province | Xie H.,The Peoples Hospital of Guizhou Province | Yue Z.,The Peoples Hospital of Guizhou Province
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology | Year: 2013

OBJECTIVE: This study aimed to assess the relationship between the distributions of Haemophilus aggregatibacter (H. aggregatibacter ), Porphyromonas gingivalis (P. gingivalis ), Prevotella intermedia (P. intermedia), Tannerella forsythensis (T. forsythensis), and Treponema denticola (T. denticola) in subgingival plaque and different periodontal conditions of chronic periodontitis.METHODS: Twenty patients (80 sites) with chronic periodontitis and ten healthy subjects (20 sites) were included. The study sites were distributed into different groups according to the differences in their pocket probing depths (PD). The groups were described as follows: group A, PD< or = 4 mm; group B, 4 mm < PD < or = 6 mm; group C, PD>6 mm. Semi-quantification of the subgingival microorganism samples was analyzed by polymerase chain reaction (PCR) and reverse hybridization assay.RESULTS: The prevalence rates and quantities of P. gingivalis, P. intermedia, T. forsythensis, and T. denticola were significantly higher in groups B and C than in the healthy group. Higher prevalence rates and quantities of P. gingivalis were detected in group A than in the healthy group. The quantities of T. forsythensis and T. denticola were also significantly higher in group C than in group B. However, the prevalence rates and quantities ofH. aggregatibacter showed no significant difference among the groups.CONCLUSION: The prevalence rates and levels ofP. gingivalis, P. intermedia, T. forsythensis, and T. denticola possibly increased as the depths of the periodontal pockets increased. The quantity ofP. gingivalis was correlated with the early stage of chronic periodontitis. The quantities of T. forsythensis and T. denticola were associated with local development of moderate or severe chronic periodontitis.

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