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Liang Y.-X.,Beijing Tsinghua Changgeng Hospital | Nong B.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Liang L.-X.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region
World Chinese Journal of Digestology | Year: 2015

AIM: To assess the clinical effects of endoscopic retrograde cholangiopancreatography (ERCP) in the treatment of choledocholithiasis with ectopic duodenal papilla. METHODS: Clinical cases of choledocholithiasis were collected through the hospital’s database to identify the cases with ectopic papilla of Vater. Based on the location of duodenal papilla, the safety and efficacy of ERCP were analyzed. RESULTS: The total number of choledocholithiasis patients who had undergone ERCP was 968 cases, and the number of cases with ectopic duodenal papilla was 6 (0.62%). Of the 6 patients, 2 underwent surgery, 3 were treated by biliary stent placement, and 1 had successful stone removal. CONCLUSION: Patients suffering from choledocholithiasis with ectopic duodenal papilla can be treated by ERCP, and surgery can be considered a useful alternative. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Zheng M.,Guangxi Medical University | Wei J.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Tang Y.,Guangxi Medical University | Yang C.,Guangxi Medical University | And 3 more authors.
Journal of Molecular Neuroscience | Year: 2014

Disruption of the blood–brain barrier (BBB) is a surrogate marker of acute inflammatory lesions in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Data from experiments suggest that apolipoprotein E (ApoE) plays an important role in the antiinflammatory and immunological process in MS/EAE. Recent researches have shown that lack of ApoE leads to loss of cerebrovascular integrity and BBB breakdown causing neuronal injury. Cerebrovascular effects of ApoE might be another important element resulting to more susceptibility to MS/EAE. However, there is no direct evidence that ApoE dependently contributes to maintaining BBB integrity in EAE. In this study, we induced EAE in ApoE−/− mice and wild-type mice. During EAE, our results show that lack of ApoE increased the Evan’s blue (EB) permeability of BBB. Furthermore, deficiency of ApoE upregulated MMP-9 expression activity but decreased the expression of endothelial cell tight junction integral proteins claudin-5 and occludin. Our result also suggests that the protective role of ApoE in EAE by maintaining BBB integrity could be another interesting therapeutic target at MS/EAE. © 2014, Springer Science+Business Media New York.


Li B.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Wang Z.,Wuhan University | Zhong Y.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Lan J.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | And 2 more authors.
Medical Oncology | Year: 2015

CC chemokine receptor-9 (CCR9) is highly expressed in non-small cell lung cancer (NSCLC) tissues and cell lines. However, the biological functions and the signals elicited by the interaction between CCR9 and its natural ligand CCL25 in NSCLC are unknown. Here, we selectively depleted CCR9 and inhibited CCR9–CCL25 interaction in NSCLC cells using small recombinant lentivirus-mediated miRNA, and investigated the tumorigenic effects in vitro and in vivo. Compromised CCR9–CCL25 interaction promoted apoptosis in NSCLC cells by activating phosphoinositide 3-kinase (PI3K)/Akt in vitro. In addition, we showed that CCR9–CCL25 interaction mediated the activation of the PI3K/Akt pathway in NSCLC cells, resulting in the up-regulation of anti-apoptotic proteins, as well as the down-regulation of apoptotic proteins in a PI3K-/Akt-dependent manner. These CCR9–CCL25-mediated effects were abrogated in the presence of a PI3K inhibitor (wortmannin 10 nM) or by inhibiting the CCR9–CCL25 interaction through CCR9 silencing, which also suggested that the biological function of CCR9–CCL25 was mainly regulated by PI3K. In vivo studies also demonstrated a significantly lower tumor burden in mice receiving CCR9-silence cells than those in mice receiving control cells. Together, these data suggested that CCR9–CCL25 interaction induced tumorigenesis of NSCLC cells and that this induction might be accomplished through the activation of the PI3K/Akt pathway. These findings may lead to a better understanding of the biological effects of CCR9–CCL25 interaction and provide clues for identifying novel therapeutic and preventive molecular markers for NSCLC. © 2015, Springer Science+Business Media New York.


Li G.,Guangxi Medical University | Shen Q.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Li C.,Guangxi Medical University | Li D.,Guangxi Medical University | And 2 more authors.
Clinical and Translational Oncology | Year: 2015

Background: MicroRNAs (miRNAs) in body fluids such as serum and plasma can be stably detected and used as potential biomarkers in hepatocellular carcinoma (HCC) diagnosis. Objective: To systematically evaluate circulating miRNAs from HCC expression profiling studies and to determine miRNA biomarkers for HCC detection. Methods: A systematic review and meta-analysis of published studies were carried out for comparing the circulating miRNA expressions between HCC patients and healthy people, hepatitis, or cirrhosis patients. A miRNA ranking system that considered the number of comparisons in agreement and total number of samples was used. Then the summary receiver-operating characteristic curve (sROC) results of the top miRNAs were combined to further evaluate their diagnostic value using Meta-disc 1.4. Results: In the 17 included studies, three circulating miRNAs (miR-21, miR-122, and miR-223) were repeatedly reported three times or more in both HCC patients vs. healthy controls and vs. other hepatitis or cirrhosis patients. In further analysis, the area under curve (AUC) of sROC for miR-21, miR-122 and miR-223 in discriminating HCC patients from healthy people are 0.9293, 0.8128, and 0.8597, respectively. Conclusions: Circulating miR-21 has highest level of diagnostic efficiency among three miRNAs candidate biomarkers (miR-21, miR-122, and miR-223) for detection of HCC. © 2015, Federación de Sociedades Españolas de Oncología (FESEO).


Zeng K.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Zheng W.,Renmin University of China | Mo X.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Liu F.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | And 4 more authors.
Archives of Gynecology and Obstetrics | Year: 2015

Purpose: MicroRNAs (miRNAs) exhibit dysregulated expression in human cancer and play an important role in carcinogenesis. The aim of this study was to identify a distinct miRNA expression signature for cervical cancer and cervical intraepithelial neoplasia (CIN) and to investigate the function of deregulated miRNAs in cervical carcinoma. Methods: A miRNA microarray was used to compare miRNA expression profiles in cervical cancer, CIN and normal cervical tissues. Real-time RT-PCR was used to validate the expression of 9 miRNAs in 103 cervical tissues. Bioinformatics programs were used to predict potential target genes and their function. Functional studies were performed to characterize the effect on cervical cancer cells by overexpression of miR-218 and miR-21. Results: We identified deregulated miRNAs in cervical cancer and high-grade squamous intraepithelial lesions (HSIL). MiR-218 was the most downregulated (0.175-fold decrease) miRNA, and miR-21 was the most upregulated (5.67-fold increase) miRNA. In addition, the expression patterns of 9 miRNAs were validated by real-time RT-PCR. Bioinformatics analyses and functional studies indicated that miR-218 and miR-21 may be involved in cancer invasion and metastasis. Conclusion: Our study demonstrated that miRNAs are aberrantly expressed in cervical cancer and cervical preneoplastic lesions. These miRNAs may be involved in the progression of cervical neoplasm as potential tumor suppressor genes or oncogenes. © 2015, Springer-Verlag Berlin Heidelberg.


Hu G.,Renmin University of China | Lin H.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Liu X.-Y.,Renmin University of China | Wang E.-Y.,Renmin University of China | Xia Z.-Y.,Renmin University of China
International Journal of Clinical and Experimental Medicine | Year: 2016

Growing evidence has highlighted the contribution of gastrointestinal ischemia/reperfusion (IR) in the process of acute lung injury. This study aims to investigate effect of Shenfu injection on preventing and treating lung injury caused by intestinal ischemia/reperfusion. Effect of Shenfu injection on levels of tumor necrosis factor-α, inducible nitric oxide synthase and intercellular adhesion molecule-1 was also determined. An intestinal ischemia/ reperfusion model was established. Sprague-Dawley rats were randomly divided into ischemia/reperfusion, normal control, and Shenfu groups. Blood gases and blood lactate, lung wet/dry weight ratio, and myeloperoxidase activity were detected. Mean arterial pressure was monitored before and after reperfusion. The amount of tumor necrosis factor-α in plasma and lung tissue was determined using enzyme-linked immunosorbent assay. Inducible nitric oxide synthase and intercellular adhesion molecule-1 expression in lung and intestinal tissue were detected by immunohistochemistry. Shenfu injection significantly attenuated pathological damage in lung caused by intestinal ischemia/reperfusion, improved oxygenation in lungs, and reduced lung wet/dry weight ratio and myeloperoxidase activity. After Shenfu injection, tumor necrosis factor-α contents in plasma and lung tissue, and expressions of inducible nitric oxide synthase and intercellular adhesion molecule-1 caused by intestinal ischemia/reperfusion, were inhibited. The mean arterial pressure in ischemia/reperfusion group after reperfusion was significantly decreased compared to Shenfu group. In conclusion, Shenfu injection could prevent occurrence of lung injury caused by intestinal ischemia/reperfusion and accordingly prevented development of multiple organ dys-function. This effect is achieved through inhibiting release of tumor necrosis factor-α, inducible nitric oxide synthase and intercellular adhesion molecule-1. © 2016, E-Century Publishing Corporation. All rights reserved.


Zhu Y.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Yuan Y.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Huang H.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Huang H.,Roswell Park Cancer Institute
International Journal of Clinical and Experimental Medicine | Year: 2015

Purpose: This study observed the relationship between procalcitonin (PCT) and results of sputum culture, the relationship between PCT and results of blood culture to evaluate and compare the value of PCT in respiratory and bloodstream infections. Methods: We analyzed 1616 patients in which PCT and sputum culture were concurrently ordered and analyzed, and 1096 patients in which PCT and blood culture were concurrently ordered and analyzed from January 2014 to May 2015. PCT concentrations were measured by on a Roche Cobas E601 ECL analyzer. Results: The average values of PCT from patients with sputum culture positive and negative were 0.42 (0.17-2.16) and 0.12 (0.06-0.57) ng/ml respectively, and the average values of PCT from patients with blood culture positive and negative were 9.54 (2.10-48.47) and 0.28 (0.10-1.23) ng/ml respectively. In sputum culture, positive rate of PCT in cases with growth of pathogens was 47.1%. In blood culture, positive rate of PCT in cases with growth of pathogens was 89.2%. Conclusions: PCT is useful in early diagnosis of respiratory infections and bloodstream infections, but the specificity of PCT in diagnosing respiratory infections is not as high as it is in bloodstream infections. © 2015, E-Century Publishing Corporation. All rights reserved.


Mu X.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Mu X.,Guangxi University | Wei J.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Li P.,Guangxi University
International Journal of Clinical and Experimental Medicine | Year: 2015

The purpose of this study was to compare the safety and efficacy of micro-endoscopic discectomy (MED) and open discectomy (OD) for lubmar disc herniation (LDH). Randomised controlled trials (RCTs) comparing MED with OD for LDH were searched comprehensively in PubMed, EMBASE, the Cochrane Library. Relevant studies retrieved, data extracted and the quality of included studies were independently performed by two authors. RevMan software (Version 5.2.0) was used to analyse and synthesis relevant data of the included studies. Nine RCTs involving 774 patients were obtained and reported the relevant outcome measures. Compared with OD group, there were significant difference in the general operation indicators including operation time, blood loss, site of incision, hospital stay and time of return to work, biochemical indexes including C-reactive protein (CRP) and interleukin-6 (IL-6) in MED group. Meanwhile, there were no difference in effective rate, complication including total complications, dural leaks occurred and recurrence of the disc herniation, compared MED group with OD group. MED had slighter trauma, milder blood loss and shorter healing time than OD. The results demonstrated MED has great efficacy and safety comparable to OD. So we think that MED can be used routinely for LDH patients, especially the patients of old and intolerable major surgery. Meanwhile, it is necessary for surgeon to master indication and contraindication of MED and improve the operative technique. © 2015, International Journal of Clinical and Experimental Medicine. All rights reserved.


Wei B.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Huang Q.,Guangxi University | Zhong X.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region
OncoTargets and Therapy | Year: 2015

Objective: Nucleostemin (NS) is a new protein localized in the nucleolus of most stem cells and tumor cells, which regulates their self-renewal and cell cycle progression. The aim of this study was to investigate the expression of NS in colorectal cancer (CRC) and the effects of NS knockdown in the Sw620 cell line to provide basis for clinical target therapy. Methods: NS expression in 372 patients with CRC and 367 normal participants was assessed using immunohistochemistry. The expression level of NS gene was evaluated by polymerase chain reaction. Then, the relationship among NS expression, clinicopathological features, and prognosis was analyzed. Silencing of NS expression was achieved by using NS-specific small-interfering RNAs. The viability and growth rate of Sw620 cells were determined by proliferation and invasion assays. Cell cycle distribution of the cells was analyzed by flow cytometry. Results: High NS expression was positively related with node metastasis, distant metastasis, and TNM stage. In Kaplan–Meier survival analysis, patients with low NS expression always had significantly longer survival time than those with high expression. Moreover, our results showed that knockdown of NS expression inhibited proliferation and viability of Sw620 cells in a time-dependent manner. Cell cycle studies revealed that NS depletion resulted in G1 cell cycle arrest at short times of transfection (24hours), followed with apoptosis at longer times (48hours and 72hours), suggesting that post-G1 arrest apoptosis occurred in Sw620 cells. Conclusion: Overall, these results point to the essential role of NS in Sw620 cells; thus, this gene might be considered a promising target for treatment of CRC. © 2015 Wei et al.


Zheng H.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Nong Z.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Lu G.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region
Medical Science Monitor | Year: 2015

Background: Nuclear factor E2-related factor 2 (Nrf2) plays an anti-oxidative and phase II detoxification function via its upregulation on various antioxidant response elements (ARE) genes. Nrf2 can protect both normal and cancer cells from damages of cell stress, thereby exerting a critical role in the development of cancer. The expression and significance of Nrf2 in gastric cancer, however, has not been reported. This study thus aimed to investigate the expression of Nrf2 in gastric cancer tissues via immunohistochemical (IHC) staining. Material/Methods: Gastric carcinoma tissues from a total of 175 patients during surgical resection were examined for Nfr2 expression profiles using IHC staining on paraffin-embedded slides. Between-group-comparisons were performed by chi-square, Fisher’s exact, or Mann-Whitney U test. The correlation between Nfr2 expression and clinical indexes was further analyzed by Kaplan-Meier test, univariate/multivariate analysis, and log-rank test. Results: Nrf2 is mainly expressed in nuclei of gastric carcinoma tissues, with significant correlation with clinical indexes, including tumor size, invasive depth, lymph node metastasis, and invasion. Patients with Nrf2-positive expression had significantly lower survival rates compared to those in the negative group (p<0.01), with chemoresistance against 5-fluorouracil (5-FU) (p<0.05). Conclusions: Nrf2 expression is positively correlated with invasive gastric cancer, suggesting its utility as a predictive index for unfavorable prognosis. © Med Sci Monit, 2015.

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