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Wei B.,Peoples Hospital of Guangxi Autonomous Region | Huang Q.,Guangxi University | Zhong X.,The Peoples hospital of guangxi Zhuang autonomous region
OncoTargets and Therapy | Year: 2015

Objective: Nucleostemin (NS) is a new protein localized in the nucleolus of most stem cells and tumor cells, which regulates their self-renewal and cell cycle progression. The aim of this study was to investigate the expression of NS in colorectal cancer (CRC) and the effects of NS knockdown in the Sw620 cell line to provide basis for clinical target therapy. Methods: NS expression in 372 patients with CRC and 367 normal participants was assessed using immunohistochemistry. The expression level of NS gene was evaluated by polymerase chain reaction. Then, the relationship among NS expression, clinicopathological features, and prognosis was analyzed. Silencing of NS expression was achieved by using NS-specific small-interfering RNAs. The viability and growth rate of Sw620 cells were determined by proliferation and invasion assays. Cell cycle distribution of the cells was analyzed by flow cytometry. Results: High NS expression was positively related with node metastasis, distant metastasis, and TNM stage. In Kaplan–Meier survival analysis, patients with low NS expression always had significantly longer survival time than those with high expression. Moreover, our results showed that knockdown of NS expression inhibited proliferation and viability of Sw620 cells in a time-dependent manner. Cell cycle studies revealed that NS depletion resulted in G1 cell cycle arrest at short times of transfection (24hours), followed with apoptosis at longer times (48hours and 72hours), suggesting that post-G1 arrest apoptosis occurred in Sw620 cells. Conclusion: Overall, these results point to the essential role of NS in Sw620 cells; thus, this gene might be considered a promising target for treatment of CRC. © 2015 Wei et al.

Hu G.,Renmin University of China | Lin H.,The Peoples hospital of guangxi Zhuang autonomous region | Liu X.-Y.,Renmin University of China | Wang E.-Y.,Renmin University of China | Xia Z.-Y.,Renmin University of China
International Journal of Clinical and Experimental Medicine | Year: 2016

Growing evidence has highlighted the contribution of gastrointestinal ischemia/reperfusion (IR) in the process of acute lung injury. This study aims to investigate effect of Shenfu injection on preventing and treating lung injury caused by intestinal ischemia/reperfusion. Effect of Shenfu injection on levels of tumor necrosis factor-α, inducible nitric oxide synthase and intercellular adhesion molecule-1 was also determined. An intestinal ischemia/ reperfusion model was established. Sprague-Dawley rats were randomly divided into ischemia/reperfusion, normal control, and Shenfu groups. Blood gases and blood lactate, lung wet/dry weight ratio, and myeloperoxidase activity were detected. Mean arterial pressure was monitored before and after reperfusion. The amount of tumor necrosis factor-α in plasma and lung tissue was determined using enzyme-linked immunosorbent assay. Inducible nitric oxide synthase and intercellular adhesion molecule-1 expression in lung and intestinal tissue were detected by immunohistochemistry. Shenfu injection significantly attenuated pathological damage in lung caused by intestinal ischemia/reperfusion, improved oxygenation in lungs, and reduced lung wet/dry weight ratio and myeloperoxidase activity. After Shenfu injection, tumor necrosis factor-α contents in plasma and lung tissue, and expressions of inducible nitric oxide synthase and intercellular adhesion molecule-1 caused by intestinal ischemia/reperfusion, were inhibited. The mean arterial pressure in ischemia/reperfusion group after reperfusion was significantly decreased compared to Shenfu group. In conclusion, Shenfu injection could prevent occurrence of lung injury caused by intestinal ischemia/reperfusion and accordingly prevented development of multiple organ dys-function. This effect is achieved through inhibiting release of tumor necrosis factor-α, inducible nitric oxide synthase and intercellular adhesion molecule-1. © 2016, E-Century Publishing Corporation. All rights reserved.

Liang Y.-X.,Beijing Tsinghua Changgeng Hospital | Nong B.,The Peoples hospital of guangxi Zhuang autonomous region | Liang L.-X.,The Peoples hospital of guangxi Zhuang autonomous region
World Chinese Journal of Digestology | Year: 2015

AIM: To assess the clinical effects of endoscopic retrograde cholangiopancreatography (ERCP) in the treatment of choledocholithiasis with ectopic duodenal papilla. METHODS: Clinical cases of choledocholithiasis were collected through the hospital’s database to identify the cases with ectopic papilla of Vater. Based on the location of duodenal papilla, the safety and efficacy of ERCP were analyzed. RESULTS: The total number of choledocholithiasis patients who had undergone ERCP was 968 cases, and the number of cases with ectopic duodenal papilla was 6 (0.62%). Of the 6 patients, 2 underwent surgery, 3 were treated by biliary stent placement, and 1 had successful stone removal. CONCLUSION: Patients suffering from choledocholithiasis with ectopic duodenal papilla can be treated by ERCP, and surgery can be considered a useful alternative. © 2015 Baishideng Publishing Group Inc. All rights reserved.

Mu X.,The Peoples hospital of guangxi Zhuang autonomous region | Mu X.,Guangxi University | Wei J.,The Peoples hospital of guangxi Zhuang autonomous region | Li P.,Guangxi University
International Journal of Clinical and Experimental Medicine | Year: 2015

The purpose of this study was to compare the safety and efficacy of micro-endoscopic discectomy (MED) and open discectomy (OD) for lubmar disc herniation (LDH). Randomised controlled trials (RCTs) comparing MED with OD for LDH were searched comprehensively in PubMed, EMBASE, the Cochrane Library. Relevant studies retrieved, data extracted and the quality of included studies were independently performed by two authors. RevMan software (Version 5.2.0) was used to analyse and synthesis relevant data of the included studies. Nine RCTs involving 774 patients were obtained and reported the relevant outcome measures. Compared with OD group, there were significant difference in the general operation indicators including operation time, blood loss, site of incision, hospital stay and time of return to work, biochemical indexes including C-reactive protein (CRP) and interleukin-6 (IL-6) in MED group. Meanwhile, there were no difference in effective rate, complication including total complications, dural leaks occurred and recurrence of the disc herniation, compared MED group with OD group. MED had slighter trauma, milder blood loss and shorter healing time than OD. The results demonstrated MED has great efficacy and safety comparable to OD. So we think that MED can be used routinely for LDH patients, especially the patients of old and intolerable major surgery. Meanwhile, it is necessary for surgeon to master indication and contraindication of MED and improve the operative technique. © 2015, International Journal of Clinical and Experimental Medicine. All rights reserved.

Li B.,The Peoples hospital of guangxi Zhuang autonomous region | Wang Z.,Wuhan University | Zhong Y.,The Peoples hospital of guangxi Zhuang autonomous region | Lan J.,The Peoples hospital of guangxi Zhuang autonomous region | And 2 more authors.
Medical Oncology | Year: 2015

CC chemokine receptor-9 (CCR9) is highly expressed in non-small cell lung cancer (NSCLC) tissues and cell lines. However, the biological functions and the signals elicited by the interaction between CCR9 and its natural ligand CCL25 in NSCLC are unknown. Here, we selectively depleted CCR9 and inhibited CCR9–CCL25 interaction in NSCLC cells using small recombinant lentivirus-mediated miRNA, and investigated the tumorigenic effects in vitro and in vivo. Compromised CCR9–CCL25 interaction promoted apoptosis in NSCLC cells by activating phosphoinositide 3-kinase (PI3K)/Akt in vitro. In addition, we showed that CCR9–CCL25 interaction mediated the activation of the PI3K/Akt pathway in NSCLC cells, resulting in the up-regulation of anti-apoptotic proteins, as well as the down-regulation of apoptotic proteins in a PI3K-/Akt-dependent manner. These CCR9–CCL25-mediated effects were abrogated in the presence of a PI3K inhibitor (wortmannin 10 nM) or by inhibiting the CCR9–CCL25 interaction through CCR9 silencing, which also suggested that the biological function of CCR9–CCL25 was mainly regulated by PI3K. In vivo studies also demonstrated a significantly lower tumor burden in mice receiving CCR9-silence cells than those in mice receiving control cells. Together, these data suggested that CCR9–CCL25 interaction induced tumorigenesis of NSCLC cells and that this induction might be accomplished through the activation of the PI3K/Akt pathway. These findings may lead to a better understanding of the biological effects of CCR9–CCL25 interaction and provide clues for identifying novel therapeutic and preventive molecular markers for NSCLC. © 2015, Springer Science+Business Media New York.

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