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Shao J.,The Wuxi Peoples Hospital | Xin Y.,Jiangnan University | Yao Y.,The Wuxi Peoples Hospital | Zhu J.,The Wuxi Peoples Hospital
Clinica Chimica Acta

Background: In the development of high myopia, some secondary ocular diseases such as macular detachment (MD) and macular hole (MH) may occur owing to the elongation of the eyeball. Higher concentrations of misfolded transthyretin (TTR) had been detected in abnormal vitreous humor, but the mechanisms are still unclear. Method: TTRs of high myopia and healthy vitreous were purified with TTR polyclonal antibody-Sepharose. Gel exclusion chromatography, cleavage activity assay and a fluorescent probe were employed for the functional comparison of natural and abnormal TTRs. Results: Compared with natural transthyretin, MH TTR showed lower retinol-binding protein (RBP) binding ability; and MD TTR could not bind with RBP at all. Additionally, MH and MD TTR did not reveal cleavage activity against apolipoprotein AI (apoA-I). Furthermore, the kinetic parameters of the interactions between abnormal TTRs and a thyroxine-like fluorescent probe were quite different from those of natural TTR. Conclusions: The results suggested that misfolded TTRs in MD and MH patients' vitreous completely or partially lost natural bio-functions; and this should be associated with abnormal high TTR levels. © 2012 Elsevier B.V. Source

Zhao J.J.,The Wuxi Peoples Hospital | Feng D.H.,The Wuxi Peoples Hospital | Cheng L.,The Wuxi Peoples Hospital | Lu T.,The Wuxi Peoples Hospital | Wang L.,The Wuxi Peoples Hospital
Genetics and Molecular Research

The purpose of this study was to investigate the association of the g.27667T>A genetic variant in the osteoprotegerin (OPG) gene with bone mineral density (BMD) and osteoporosis. A total of 393 primary osteoporosis subjects and 402 healthy controls were recruited. The BMD of the femoral neck hip, lumbar spine (L2-4), and total hip were evaluated by Norland XR-46 dual-energy X-ray absorptiometry. The g.27667T>A genetic variant was genotyped using created restriction site-polymerase chain reaction. Our data indicated significant differences in BMD of the femoral neck hip, lumbar spine (L2-4), and total hip among different genotypes. Individuals with the genotype TT had significantly higher BMDs than those of genotypes TA and AA (P < 0.05). Results from this study suggest that the g.27667T>A genetic variant in the OPG gene is potentially related to BMD and osteoporosis in Chinese postmenopausal women. © FUNPEC-RP. Source

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