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Xiao G.,Chinese Academy of Agricultural Sciences | Xiao G.,Pre State Key Laboratory for Germplasm Innovation and Resource Utilization of Crops | Xiao G.,The Oil Crops Research Institute National Oil Crops Improvement Center | Zhang Z.Q.,The Oil Crops Research Institute National Oil Crops Improvement Center | And 9 more authors.
Gene | Year: 2014

In the present study, we characterized the transcriptional regulatory region (KF038144) controlling the expression of a constitutive FAD2 in Brassica napus. There are multiple FAD2 gene copies in B. napus genome. The FAD2 gene characterized and analyzed in the study is located on chromosome A5 and was designated as BnFAD2A5-1. BnFAD2A5-1 harbors an intron (1192. bp) within its 5'-untranslated region (5'-UTR). This intron demonstrated promoter activity. Deletion analysis of the BnFAD2A5-1 promoter and intron through the β-glucuronidase (GUS) reporter system revealed that the 220 to 1. bp is the minimum promoter region, while 220 to 110. bp and +. 34 to +. 285. bp are two important regions conferring high-levels of transcription. BnFAD2 transcripts were induced by light, low temperature, and abscisic acid (ABA). These observations demonstrated that not only the promoter but also the intron are involved in controlling the expression of the BnFAD2A5-1 gene. The intron-mediated regulation is an essential aspect of the gene expression regulation. © 2014 Elsevier B.V. Source

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