Chiyoda-ku, Japan

The Nippon Dental University

www.ndu.ac.jp
Chiyoda-ku, Japan

The Nippon Dental University is a private university in Tokyo and Niigata, Japan, established in 1947. The predecessor of the school was founded in 1907. One out of every seven dentists in Japan is a graduate of this school.The university attracted international opprobrium in 2006 when its museum of medicine and dentistry declined to return a copy of Andreas Vesalius's De Humani Corporis Fabrica , stolen from the library of Christ Church, University of Oxford in 1995. The book was one of 74 books stolen from the library, 73 of which were subsequently recovered, with the full cooperation of libraries and dealers all over the world. http:/features203097.article Wikipedia.

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We recently indicated that brain-derived neurotrophic factor (BDNF) enhances the excitability of small-diameter trigeminal ganglion (TRG) neurons projecting onto the trigeminal nucleus interpolaris/caudalis (Vi/Vc) transition zone via a paracrine mechanism following masetter muscle (MM) inflammation. The present study investigated whether modulation of voltage-gated potassium (K) channels by BDNF contributes to this hyperexcitability effect. To induce inflammation we injected complete Freund's adjuvant (CFA) into the MM. The escape threshold from mechanical stimulation applied to skin above the inflamed MM was significantly lower than in naïve rats. TRG neurons innervating the site of inflammation were subsequently identified by fluorogold (FG) labeling, and microbeads (MB) were used to label neurons projecting specifically to the Vi/Vc region. BDNF significantly decreased the total, transient (IA), and sustained (IK) currents in FG-/MB-labeled small-diameter TRG neurons under voltage-clamp conditions in naïve and inflamed rats. The magnitude of inhibition of IA and IK currents by BDNF in FG-/MB-labeled TRG neurons was significantly greater in inflamed rats than in naïve rats, and BDNF inhibited IA to a significantly greater extent than IK. Furthermore, co-administration of K252a, a tyrosine kinase inhibitor, abolished the suppression of IA and IK currents by BDNF. These results suggested that the inhibitory effects of BDNF on IA and IK currents in small-diameter TRG neurons projecting onto the Vi/Vc potentiate neuronal excitability, and in turn, contribute to MM inflammatory hyperalgesia. These findings support the development of voltage-gated K+ channel openers and tyrosine kinase inhibitors as potential therapeutic agents for the treatment of trigeminal inflammatory hyperalgesia. © 2014 IBRO.


Patent
Hitachi Ltd. and The Nippon Dental University | Date: 2012-08-09

Provided is a medicinal agent for medical applications, which can act on the function of a target cell specifically. The medicinal agent for medical applications comprises: a cell-incorporated substance that can be incorporated into a target cell specifically; and an acting substance that can act on the function of the target cell and is bound to the cell-incorporated substance.


Patent
The Nippon Dental University and Hitachi Ltd. | Date: 2014-06-18

Provided is a medicinal agent for medical applications, which can act on the function of a target cell specifically. The medicinal agent for medical applications comprises: a cell-incorporated substance that can be incorporated into a target cell specifically; and an acting substance that can act on the function of the target cell and is bound to the cell-incorporated substance.


Ono H.,The Nippon Dental University | Miyamoto A.,High Energy Accelerator Research Organization
European Physical Journal C | Year: 2013

Precise measurement of the Higgs boson couplings is an important task for International Linear Collider (ILC) experiments and will clarify the understanding of the particle mass generation mechanism. In particular, high precision measurement of Higgs branching ratios plays a key role in the search for the origin of the Yukawa and Higgs interactions. In this study, the measurement accuracies of Higgs boson branching ratios to b and c quarks and gluons were evaluated using a full detector simulation based on the International Large Detector, and assuming a Higgs mass of 120 GeV/c2. We analyze two center-of-mass (CM) energies, 250 and 350 GeV, close to the e+e-→ZH and e+e- → tt̄ production thresholds. At both energies, an integrated luminosity of 250 fb-1 and an electron (positron) beam polarization of -80 % (+30 %) were assumed. We obtain the following measurement accuracies for the product of the Higgs production cross section and the branching ratio of the Higgs into bb̄, cc̄, and gg: 1. 0 %, 6. 9 %, and 8. 5 % at a CM energy of 250 GeV and 1. 0 %, 6. 2 %, and 7. 3 % at 350 GeV. (After writing our article, Large Hadron Collider experiments reported the observation of a new resonance around the mass of 125 GeV/c2 (ATLAS Collaboration, arXiv:1207. 7214v1 [hep-ex]; CMS Collaboration, arXiv:1207. 7235v1 [hep-ex]). Considering the small difference in branching ratios of the Higgs at masses of 120 and 125 GeV/c2, our results are not significantly affected by this mass difference.) © 2013 The Author(s).


Kanazawa T.,The Nippon Dental University | Matsumoto S.,The Nippon Dental University
Brain Research Bulletin | Year: 2014

Transient receptor potential vanilloid 1 (TRPV1) is a polymodal sensor that is activated by heat (>43°C), acid, or capsaicin, the pungent ingredient of hot peppers. Reports that mice lacking TRPV1 display heat avoidance behaviors and TRPV1-negative neurons respond to heat suggest that an additional heat sensor is present. Anoctamin 1 (ANO1; also known as transmembrane protein 16A [TMEM16A]), is a component of Ca2+-activated chloride channels (CaCCs), and has been recently identified as a heat sensor, activated by temperatures over 44°C. ANO1 is highly co-localized with TRPV1 in small-diameter dorsal root ganglion (DRG) neurons. The aim of the present study was to investigate co-expression of ANO1 and TRPV1 in rat trigeminal ganglion (TG) neurons innervating the tongue by using retrograde labeling and immunohistochemical techniques. Fluoro-gold (FG) retrograde labeling was used to identify the TG neurons innervating the anterior two thirds of the tongue; as expected, most labeling was detected in the mandibular division of the TGs. The FG-labeled TG neurons showed TRPV1 immunoreactivity (17.9%) and ANO1 immunoreactivity (13.7%), indicating that TRPV1- and ANO1-expressing neurons were present in the mandibular division of the TGs. Seventy-six percent of the ANO1-immunoreactive TG neurons were also immunoreactive for TRPV1; this co-expression was mainly detected in small- to medium-diameter TG neurons. The high degree of co-expression of TRPV1 and ANO1 suggests that cooperation between ANO1 and TRPV1 plays a role in the signaling pathways of nociceptive TG neurons. © 2014 Elsevier Inc.


Lin J.,The Nippon Dental University
International journal of oral science | Year: 2010

AIM: To evaluate the interactive effects of different self-adhesive resin cements and tribochemical treatment on bond strength to zirconia. METHODOLOGY: The following self-adhesive resin cements for bonding two zirconia blocks were evaluated: Maxcem (MA), Smartcem (SM), Rely X Unicem Aplicap (UN), Breeze (BR), Biscem (BI), Set (SE), and Clearfil SA luting (CL). The specimens were grouped according to conditioning as follows: Group 1, polishing with 600 grit polishing paper; Group 2, silica coating with 110 microm Al2O3 particles which modified with silica; and, Group 3, tribochemical treatment--silica coating + silanization. Specimens were stored in distilled water at 37 degrees C for 24 hours before testing shear bond strength. RESULTS: Silica coating and tribochemical treatment significantly increased the bond strength of the MA, UN, BR, BI, SE and CL to zirconia compared to #600 polishing. For both #600 polished and silica coating treatments, MDP-containing self-adhesive resin cement CL had the highest bond strengths to zirconia. CONCLUSION: Applying silica coating and tribochemical treatment improved the bond strength of self-adhesive resin cement to zirconia, especially for CL.


Tsuchimochi M.,The Nippon Dental University | Hayama K.,The Nippon Dental University
Physica Medica | Year: 2013

Several small gamma cameras (SGCs) intended for surgical use are now in development or currently being marketed. In this review, we discuss the characteristics, performance, and clinical use of SGCs which are hand-held and small enough to be easily managed by surgeons during their procedures. We expect that SGCs have the potential to be used more widely in radioguided surgery. As advancing molecular imaging technologies will broaden clinical indications, SGCs will likely be used and integrated with other imaging modalities into numerous types of radioguided surgery in the near future. © 2012 Associazione Italiana di Fisica Medica.


Patent
The Nippon Dental University | Date: 2011-06-08

Provided are a method for forming at least a tooth root in a tooth containing a tooth crown through in vitro culturing; i.e., without using xenotransplantation or allotransplantation techniques (in vivo culturing); and a regenerated tooth obtained by the method. This method is a method for forming at least a tooth root in a tooth containing a tooth crown, the method including forming a culture core containing the tooth and a cell-containing base material, the tooth being wrapped with the cell-containing base material, and culturing the culture core in a medium so as to form at least the tooth root in the tooth contained in the culture core, wherein the cell-containing base material contains at least one kind of cells selected from periodontal ligament-derived cells, bone marrow-derived cells, dental follicle-derived cells, dental pulp-derived cells and dental papilla-derived cells and wherein the medium contains predetermined components.


Patent
The Nippon Dental University | Date: 2011-03-04

A method for forming at least a tooth root in a tooth containing a tooth crown, including: forming a culture core containing the tooth and a cell-containing base material, the tooth being wrapped with the cell-containing base material, and culturing the culture core in a medium to form at least the tooth root in the tooth contained therein, wherein the cell-containing base material contains at least one kind of cells selected from periodontal ligament-derived cells, bone marrow-derived cells, dental follicle-derived cells, dental pulp-derived cells and dental papilla-derived cells, and the medium contains a component contained in a conditioned medium of a serum-free-cultured cell line of a human uterocervical squamous carcinoma cell line; an additive containing at least one selected from IL-1, IL-6, IL-8, IL-9, EGF, IGF-I, GH, PDGF-AB, VEGF, LIF, HGF, FGF-2, FGF-1, BMP-2, BMP-4, M-CSF, dexamethasone, insulin, thyroxine, thyrocalcitonin, ascorbic acid and -glycerophosphate; or both of them.


Saiki K.,The Nippon Dental University | Konishi K.,The Nippon Dental University
Molecular Oral Microbiology | Year: 2014

Outer membrane protein PG27 is essential for secretion/maturation of conserved C-terminal domain (CTD) proteins such as gingipains, HagA, and PG0026. To determine the binding partner(s) of PG27, we used a Porphyromonas gingivalis mutant strain, 83K48, which expressed functional histidine-tagged PG27. Purification of histidine-tagged PG27 from 83K48 found that 136-kDa and 264-kDa proteins accompanied histidine-tagged PG27. Mass spectrometry revealed the 136-kDa protein and 264-kDa protein to be PG0026 and PG1837 (HagA), respectively. PG0026 is a C-terminal signal peptidase which cleaves the CTDs of other CTD proteins. A cross-linking and a native electrophoresis studies suggested the interaction between histidine-tagged PG27 and HagA and the interaction between histidine-tagged PG27 and PG0026. We constructed Porphyromonas gingivalis gene disrupting mutants, and characterized them. PG0026 was required for the full activities of gingipains, whereas HagA was not. A mutation disrupting PG0026 affected localization of PG27, but a mutation disrupting PG1837 showed little effect on the expression and localizations of PG27 and PG0026. To determine the functional role of HagA, we used PG1837-disruptant 83K54 which expressed functional histidine-tagged PG27. Histidine-tagged PG27 in 83K54 was co-purified with not only PG0026 but also several contaminated proteins. These results suggest that PG0026 interacts with PG27 in the absence of HagA, and that the binding state of a PG27-PG0026 complex was affected and stabilized by HagA. Taken together, these data suggest that secreted PG0026 anchors to the cell by interacting with PG27, is stabilized by HagA, and functions in processing of other CTD proteins such as gingipains. © 2013 John Wiley & Sons A/S.

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