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Fu X.,Stevens Institute of Technology | Fu X.,The National Engineering Research Center for Tissue Restoration and Reconstruction | Fu X.,South China University of Technology | Xu M.,Stevens Institute of Technology | And 4 more authors.
Biomaterials | Year: 2014

Nanofibrous matrices hold great promise in skin wound repair partially due to their capability of recapturing the essential attributes of native extracellular matrix (ECM). With regard to limited studies on the effect of nanofibrous matrices on keratinocytes, the present study was aimed to understand how the topographical feature of nanofibrous matrices regulates keratinocyte motility by culturing keratinocytes on polycaprolactone (PCL)/collagen nanofibrous matrices (rough surface with fiber diameters of 331±112nm) or the matrices coated with a thin layer of collagen gel to form a secondary ultrafine fibrous network (smooth surface with ultrafine fiber diameters of 55±26nm). It was found that the PCL/collagen nanofibrous matrices alone did not stimulate cell migration, while collagen gel coating could significantly increase cell motility. Further studies demonstrated that the ultrafine fibrous network of collagen gel coating significantly activated integrin β1, Rac1 and Cdc42, facilitated the deposition of laminin-332 (formerly called laminin-5), and promoted the expression of active matrix metalloproteinases (MMPs) (i.e., MMP-2 and 9). Neutralization of integrin β1 activity abrogated the gel coating-induced keratinocyte migration. These findings provide important evidence on the role of topographical features of nanofibrous matrices in regulating the phenotypic alteration of keratinocytes and suggest the possible utility of collagen-containing nanofibrous matrices for skin regeneration especially in re-epithelialization. © 2013 Elsevier Ltd. Source


Lin C.,The National Engineering Research Center for Tissue Restoration and Reconstruction | Lin C.,South China University of Technology | Lin C.,Wenzhou Medical College | Mao C.,The National Engineering Research Center for Tissue Restoration and Reconstruction | And 9 more authors.
Wuji Cailiao Xuebao/Journal of Inorganic Materials | Year: 2013

The 45S5 bioactive glass (45S5) was prepared by a melting process, while sol-gel bioactive glass (SGBG) was obtained by Sol-Gel method. The bioactive glasses were characterized by SEM, BET and XRD. Then the wound healing effect was investigated through the wound healing time, wound healing rate and histology examination. The results indicate that the bioactive glass can lessen the wound healing time and increase the healing rates of diabetic rats. Compared with the 45S5, SGBG can promote wound healing of diabetic rats more quickly and efficiently due to the larger surface area and nanostructure. Histological examination shows that bioactive glasses promote the proliferation of fibroblasts and growth of granulation tissue. All results suggest that bioactive glass can accelerate the recovery of skin wounds and SGBG with nanostructure has a better healing effect in diabetes-impaired models. Source


Mao C.,The National Engineering Research Center for Tissue Restoration and Reconstruction | Mao C.,South China University of Technology | Lin C.,The National Engineering Research Center for Tissue Restoration and Reconstruction | Lin C.,South China University of Technology | And 3 more authors.
Journal Wuhan University of Technology, Materials Science Edition | Year: 2014

In order to accelerate the chronic wounds healing, we investigated the healing effects of bioactive glass and Yunnan baiyao ointments in streptozotocin-induced diabetic rats. The ointments were prepared by mixing 45S5 bioactive glass powder (16% weight) with Vaseline and different weight percentages of Yunnan baiyao. Full-thickness defect wounds were created on the back of 130 SD rats and were randomly divided into 8 groups. The wound healing rates were calculated at 4, 7, 10, 14 and 21 days after surgery. The samples were harvested for further observations. Considering the wound closure rate, group 6 (with 5% Yunnan baiyao) has better wound healing performance than other diabetic groups. The lower inflammatory response was observed by gross observation and confirmed by the results of H&E staining and TEM observation. Besides, the proliferation of fibroblasts, the formation of granulation tissue, as well as the vascularization, were improved in group 6 compared to other diabetic groups. All results suggest that bioactive glass and Yunnan baiyao ointments can accelerate the recovery of diabetes-impaired skin wounds, and comparing to other diabetic groups, group 6 (with 5% Yunnan baiyao) has better healing effect. © 2014, Wuhan University of Technology and Springer-Verlag Berlin Heidelberg. Source

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