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Osman A.G.,The National Center for Research | Elaziz F.I.A.,University of Dongola | ElHassan G.A.,Sudan University of Science and Technology
Pakistan Journal of Nutrition | Year: 2010

A field experiment was conducted at Dongola University Farm at Al-Seleim - Dongola University Farm, Northern State to study the effects of Rhizobium Ieguminosarum biovar viceae and Bacillus megatherium var. Phosphaticum (BMP) inoculation and phosphorus and nitrogen fertilization on the yield and seed quality of faba bean (Vicia faba L.) cultivar seleim. Rhizobium inoculation significantly (p≤0.05) increased yield, seed ash, fat, crude protein and 100-seed weight. BMP inoculation significantly (p≤0.05) increased seed moisture, fat, crude fiber and crude protein content. On the other hand, Rhizobium and BMP Co-inoculation significantly (p≤0.05) increased yield, seed ash, protein content and 100-seed weight. Application of chemical fertilizers increased yield, seed ash, fat, crude protein content and 100-seed weight. In addition to that, nitrogen fertilization significantly (p≤0.05) increased the hydration coefficient. Nitrogen fertilization in the presence of Rhizobium significantly (p≤0.05) increased the cookability. © Asian Network for Scientific Information, 2010.


Osman A.G.,The National Center for Research | Abd Elaziz F.I.,University of Dongola
Advances in Environmental Biology | Year: 2010

A field experiment was conducted at Dongola University Farm at Al-Seleim to study the effects of Rhizobium leguminosarum biovar viceae and Bacillus megatherium var. Phosphaticum (BMP) inoculation and phosphorus and nitrogen fertilization on the symbiotic properties, and yield of faba bean (Vicia faba L.) cultivar seleim. Rhizobium inoculation significantly (P≤0.05) increased nodulation, shoot and nodule dry weight, nitrogen and phosphorus content in shoot and yield. BMP inoculation significantly (P≤0.05) increased nodulation, nodule, shoot and root dry weights, nitrogen and phosphorus content in shoot Rhizobium and BMP co-inoculation significantly (P≤0.05) increased nodulation, nodules, shoot and root dry weights, nitrogen and phosphorus content and yield. Application of chemical fertilizers increased shoot and root dry weight, nitrogen and phosphorus content and yield. The 4-way interaction significantly (P≤0.05) increased shoot dry weight and phosphorus content. © 2010, American-Eurasian Network for Scientific Information.


Abdalla A.S.,The National Center for Research | Osman A.G.,The National Center for Research | Abdelgani M.E.,The National Center for Research | Rugheim A.M.E.,Islamic University
Advances in Environmental Biology | Year: 2011

A field experiment was conducted at Ummdagarsi area in Gezira state, central Sudan to study the effects of inoculation with Rhizobium and Bacillus megatherium var. Phosphaticum (BMP) and phosphorus and nitrogen fertilization on yield and seed quality of chick pea plants variety Bergag. Inoculation with root nodule bacteria significantly (P≤0.05) increased nodule number, shoot dry weight, root dry weight, nodule dry weight, shoot nitrogen content and chick pea seed yield. Inoculation with phosphate solubilizing bacteria was found to cause a significant (P≤0.05) increase in nodule number, dry weight of shoot, root and nodule, shoot nitrogen and phosphorus content and yield. Results revealed that the use of chemical fertilizers significantly (P≤0.05) increased shoot dry weight, root dry weight, nodule dry weight, shoot nitrogen and phosphorus content and yield.


Osman A.G.,The National Center for Research | Rugheim A.M.E.,Islamic University | Elsoni E.M.,Ministry of Agriculture
Advances in Environmental Biology | Year: 2011

A field experiment was conducted at Elbagair area, central Sudan, for two successive seasons 2009/2010 - 2010/2011 to study the effects inoculation with five introduced or locally isolated Rhizobium or Bradyrhizobium strains each alone or with Bacillus megatherium var. phosphaticum strain (BMP) alone, and combination of each rhizobial strain with Bacillus megatherium var. phosphaticum strain (BMP) on the symbiotic properties, and yield of pigeon pea (Cajanus cajan). Rhizobium and BMP inoculation separately significantly (P≤0.05) increased nodulation, nodules dry weight, root dry weight, shoot dry weight, nitrogen and phosphorus content in shoot and seed yield of pigeon pea plants in comparison to the un-inoculated control. Rhizobium and BMP co-inoculation significantly (P≤0.05) increased nodulation, nodules, root and shoot dry weights nitrogen and phosphorus content in shoot and seed yield. The results of this study revealed the need for biological fertilizers with compatible effective strains for application to inoculate pigeon pea, especially this crop is cultivated in Sudan without fertilization.


Adam O.,The National Center for Research | Ali A.K.M.,The National Center for Research | Hubschen J.M.,La Lumiere | Muller C.P.,La Lumiere
BMC Infectious Diseases | Year: 2014

Background: Epidemiological data about congenital rubella syndrome (CRS) are scarce and rubella vaccine is not yet included in the childhood immunization schedule in Sudan. This study aimed to identify and describe CRS cases among Sudanese infants with congenital eye or heart defects.Methods: Between February and September 2010, paired oral fluid and dried blood spot samples were collected from 98 infants aged up to 12 months. These infants were enrolled during their visits to five hospitals in Khartoum, Sudan. Clinical samples were screened for rubella IgM and for ≥ 6 months old infants also for IgG antibodies by ELISA. The oral fluid of IgM and/or IgG positive patients was tested for rubella RNA by reverse transcriptase PCR.Results: Our findings revealed that two children (2.0%) were IgM positive and another five children (5.1%) were positive for IgG antibodies. None of the five infants of which enough oral fluid was available for RNA investigation was PCR positive.Conclusions: This study documented the presence of CRS in Sudan and highlighted the importance of rubella vaccine introduction for preventing future CRS cases in the country. © 2014 Adam et al.; licensee BioMed Central Ltd.


Salama Abdalla A.,The National Center for Research | Abdelgani M.E.,The National Center for Research | Osman A.G.,The National Center for Research
Pakistan Journal of Nutrition | Year: 2013

A field experiment was conducted at Ummdagarsi area in Gezira state, central Sudan to study the effects of inoculation with Rhizobium and Bacillus megatherium var. Phosphaticum (BMP), phosphorus and nitrogen fertilization on yield and seed quality of chickpea plants variety Bergag. Rhizobium inoculation significantly (p≤0.05) increased yield, ash, fiber, tannin and protein content of chickpea seeds. BMP inoculation significantly (p≤0.05) increased ash, fiber, protein content and cookability. Rhizobium and BMP co-inoculation significantly (p≤0.05) increased yield, 100 seed weight, ash, fat, protein content and cookability. Application of chemical fertilizers significantly (p≤0.05) increased yield, 100 seed weight, ash, fat and protein content. © Asian Network for Scientific Information, 2013.


Elsoni E.M.,The National Center for Research | Osman A.G.,The National Center for Research
Pakistan Journal of Nutrition | Year: 2011

A field experiment was conducted at Elbagair area, Al Jazirah state, central Sudan for two successive seasons 2009/2010-2010/2011. In these experiments pigeon pea plants were either inoculated with five introduced or locally isolated Rhizobium or bradyrhizobium strains each alone or with Bacillus Megatherium var. Phosphaticum strain (BMP) alone and combination of each rhizobial strain with Bacillus Megatherium var. Phosphaticum strain (BMP). In addition to control for comparison. Inoculation with Rhizobium significantly (p<0.05) increased pigeon pea seed yield, 100-seed weight, cookability, moisture content, ash, fat, fiber and protein. BMP inoculation significantly (p<0.05) increased seed yield, 100-seed weight, cookability, fat, fiber and protein content. Rhizobium and BMP co-inoculation significantly (p<0.05) increased seed yield, 100-seed weight, cookability, moisture, fat, fiber and protein content. Inoculation with Rhizobium strain TAL 1132 and co-inoculation with Rhizobium strain USDA 3472 and BMP as phosphobacterin was efficient to give significant yield, with good quality. © Asian Network for Scientific Information, 2011.


Moglad E.H.O.,The National Center for research | Alhassan M.S.,The National Center for research | Koko W.S.,The National Center for research | Saadabi A.M.,Neelain University
Journal of Medical Sciences (Faisalabad) | Year: 2012

The development of drug resistance as well as appearance of undesirable side effects of certain antibiotics has lead to the search for new antimicrobial agents mainly from plant extracts with the goal to discover new chemical structures. To overcome these disadvantages, the research was carried out to investigate the in-vitro antibacterial and antifungal activity of Cadaba farinose (Capparaceae), Solanum nigrum (Solanaceae), Senna occidentalis (Caesalpiniaceae) and Maerua oblongifolia (Capparaceae). Eighty percent Methanol and chloroform extracts of leaves of plants were screened for their antimicrobial activity against different pathogenic bacteria and fungi. These were Staphylococcus aureus, Bacillus subtilus, Escherichia coli, Salmonella typhi and Aspergillus niger and Candida albicans using the cup plate agar diffusion method. All methanol extracts exhibited inhibitory effects against all tested organisms with zones of inhibition ranging from 11-25 mm except the methanol extract of both parts of Maerua oblongifolia were active against Escherichia coli and Aspergillus niger. The results obtained from plants extracts were compared to some of the commercially used drugs. The Minimum Inhibitory Concentrations (MICs) of the most active extracts of these plants against standard bacteria and fungi were also determined and found that MI Cs a ranging between concentration 2.5-5 mg mL-1. All of the plants extracts were phytochemically screened and triterpenes, saponins and tannins were present in all of the methanolic extracts. Coumarins, sterols and triterpenes were found in all choloroform extracts, this finding indicated that these extracts of such plants promising antimicrobial agents.


Mohamed S.S.E.,The National Center for Research | Babiker H.M.,Ministry of Agriculture
Advances in Environmental Biology | Year: 2012

A field experiment was carried out in season 2007/08 in two sites in a semi-arid zone in the Northern State of Sudan to study the effects of Rhizobium inoculation and urea fertilization on the symbiotic properties and yield of faba bean cultivar seleim (SM-L). Rhizobium inoculation individually significantly (P≤0.05) increased shoot and nodule dry weight, nitrogen content in shoot and yield. Inoculation with Rhizobium with addition of urea as starter dose (20 kg N/ha) increased number of nodules, shoot and root dry weights, nitrogen content and yield. Urea fertilization significantly (P≤0.05) increased shoot dry weight, number of pods and yield. The results of the study indicated the efficiency of using biofertilizer based on Rhizobium isolate ENRRI 9 in increasing the yield of faba bean in the studied sites in a semi-desert zone in Sudan.


Samejima H.,Kobe University | Samejima H.,Nagoya University | Babiker A.G.,Sudan University of Science and Technology | Babiker A.G.,The National Center for Research | And 3 more authors.
Pest Management Science | Year: 2016

BACKGROUND: Purple witchweed (Striga hermonthica), Orobanchaceae, is an obligate root-parasitic weed of important cereal crops. The parasite is a copious seed producer, and a huge seed bank develops soon after the onset of the initial infestation. To germinate, a Striga seed requires a pretreatment in a moist warm environment and a subsequent exposure to an exogenous stimulant. One approach to reduce the seed bank is artificially to induce germination of the seeds in the absence of or away from the host roots. A newly developed germination stimulant for S. hermonthica, designated as T-010, was evaluated for efficacy in greenhouse and field experiments under artificial Striga infestation. RESULTS: T-010 displayed germination-inducing activity in soil. Formulated T-010 applied at 0.1, 1 and 10 kg ai ha−1 to potted soil containing S. hermonthica seeds, previously conditioned by judicious irrigation, reduced Striga emergence by 94–100%. Results of a field trial showed that formulated T-010, at the same rates as for the pot experiment, delayed and reduced Striga emergence by 33% and increased sorghum shoot and head dry weight by 18.7–40.2% and 187–241% respectively. CONCLUSION: These findings demonstrated, for the first time, the technical feasibility of suicidal germination for controlling S. hermonthica. Optimising structure, formulation and application protocol of germination stimulants should be the main goal for further improvement of the technology. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry

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