The Military General Hospital of Chengdu PLA

Chengdu, China

The Military General Hospital of Chengdu PLA

Chengdu, China
SEARCH FILTERS
Time filter
Source Type

Liu X.,Shanghai University | Lin N.,Shanghai University | Lin N.,The Military General Hospital of Chengdu PLA | Zan D.,Shanghai University | And 2 more authors.
Cell Biochemistry and Biophysics | Year: 2011

The objective is to study the effect of zymosan on antioxidant and immune function of S180 tumor-bearing mice. Seventy Kunming mice were randomly divided into seven groups: a normal control group (NC), a tumor control group (TC), three dose groups of zymosan (low, medium, high), a cyclophosphamide (Cy) group, and a combination of zymosan and Cy group. The S180 tumor-bearing mice model was established by the inoculation of cancer cell suspension subcutaneously in the mouse's right anterior limb. At the 19th day, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activity in liver homogenate were analyzed. The reverse transcriptase-polymerase chain reaction was used to determine the mRNA expression levels of IL-2, TNF-α, and TGF-β1. The activity of GSH-Px and SOD in the liver increased with the dose of zymosan, whereas the activity of MDA significantly decreased in the higher-dose groups of zymosan, compared to the TC group (P < 0.01). In the zymosan groups, mRNA expression levels in tissues of S180 tumor-bearing mice were significantly higher for TNF-α and IL-2, but lower for TGFβ1 than in the Cy or TC group (P < 0.01). The high-dose of zymosan markedly showed a depressant effect on S180 tumor, enhanced by the action of Cy that increased mRNA expression levels of TNF-α and IL-2. The mechanism of zymosan on the inhibition of tumor growth may be due to its ability to enhance the antioxidant and immune function in a dose-dependent manner. © 2011 Springer Science+Business Media, LLC.


Zhao J.,Sichuan University | Deng M.,Sichuan University | Zeng J.,Sichuan University | Huang Z.,Sichuan University | And 4 more authors.
Colloids and Surfaces A: Physicochemical and Engineering Aspects | Year: 2012

In order to obtain magnetic nanoparticles (NPs) coated with small biomolecules, histidine (His)-coated iron oxide (His-Fe 3O 4) and cobalt ferrite (His-CoFe 2O 4) with a polycrystalline structure were prepared by a simple liquid deposition method at room temperature. The growth mechanism for His-Fe 3O 4 and His-CoFe 2O 4 NPs was preliminarily explained, and their growth conditions, such as His concentrations, Co/Fe ratio, the synthesis time, were studied. The analysis of Fourier transform infrared spectra and X-ray photoelectron spectroscopy results indicated that the as-prepared particles were conjugated with the small histidine molecules. The hysteresis loops of NPs revealed their good magnetic property. L929 Cell tests indicate the good cytocompatibility of His-Fe 3O 4 and His-CoFe 2O 4 NPs, which is better than the previous prepared magnetic NPs with the polymer. © 2012 Elsevier B.V.


Yin G.,Sichuan University | Huang Z.,Sichuan University | Deng M.,Sichuan University | Zeng J.,Sichuan University | Gu J.,The Military General Hospital of Chengdu PLA
Journal of Colloid and Interface Science | Year: 2011

Silk fibroin (SF)-coated Fe 3O 4 nanoparticles (NPs) with good superparamagnetism were successfully prepared via a bio-mineralization process at room temperature. Two cell tests revealed that mineralized SF-coated Fe 3O 4 NPs presented good cytocompatibility for L929 and osteoblast cells and higher cell density after 5d with high concentrations of SF-coated Fe 3O 4 NPs (up to 0.5mg/mL). These resulted from SF surface coating on NPs, nano-surface morphology and iron ion release of Fe 3O 4 NPs. The mineralized SF-coated Fe 3O 4 NPs could be envisioned for various bone orthopedic and therapeutic applications, in which SF-coated NPs location is controlled through an external magnetic field to promoted bone growth. © 2011 Elsevier Inc.


Zhao J.,Sichuan University | Huang Z.,Sichuan University | Zeng J.,Sichuan University | Deng M.,Sichuan University | And 3 more authors.
Journal of Inorganic and Organometallic Polymers and Materials | Year: 2012

Magnetic nanoparticles (NPs) of cobalt oxide (Co 3O 4) with the diameter of 20-40 nm have been prepared by a simple liquid deposition method in the Histidine (His) assistance at room temperature. Ethanol plays an important role in the preparation of cobalt oxide NPs with a polycrystalline structure. The growth mechanism for Co 3O 4 cube particles has been preliminarily explained. The hysteresis loop of NPs reveals their good magnetic property indicating that they can be used in hyperthermia, cell separation etc. These applications need the magnetic particles with cytocompatible properties. The analysis of IR spectrum, TG curve and HRTEM image indicated that cobalt oxide particles was conjugated with the His molecules. Escherichia coli (E. coli) and L929 cells tests suggest a good cellular compatibility at a concentration of less than 0.25 mg/mL, indicating that the prepared Co 3O 4 NPs have a potential for several biomedical applications. © 2011 The Author(s).


Yan X.,Sichuan University | Yan X.,The Military General Hospital of Chengdu PLA | Huang Z.,Sichuan University | He M.,Sichuan University | And 5 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2012

Specific antibody of Human chorionic gonadotrophin (HCG) was conjugated onto hierarchical ZnO arrays through the carbodiimide technique, and the photoluminescence (PL) intensity of ZnO arrays were enhanced linearly with the linked antibody concentration in the range of 40-160. ng/mL, which resulted from the nano sheet structure and rough surface in hierarchical ZnO columns. After the specific combination between antigen and antibody on hierarchical ZnO arrays, the enhanced PL intensity of ZnO arrays was also basically linear with the concentration of HCG antigen. Thus, detection of HCG antigen in the range of 2-20. ng/mL was achieved based on PL intensity enhancement, suggesting that the prepared ZnO arrays are envisioned to be applied in the detection of early tumor markers in future. © 2011 Elsevier B.V.


Wang Y.,Sichuan University | Liao X.,Sichuan University | Huang Z.,Sichuan University | Yin G.,Sichuan University | And 2 more authors.
Colloids and Surfaces A: Physicochemical and Engineering Aspects | Year: 2010

Ni-doped ZnO nanoparticles (NPs) were successfully prepared by bioassisted synthesis technique in the template of silk-fibroin (SF) peptide at room temperature. The dopant Ni ions were incorporated into the wurtzite structure of ZnO crystal. Photoluminescence results of Ni-doped ZnO particles indicated a strong emission band at 410. nm. The field dependence of magnetization measured at room temperature exhibited the good ferromagnetic properties. Bacteriological tests revealed that the prepared Ni-doped ZnO particles with SF peptide had nontoxicity for Staphylococcus aureus and Escherichia coli. © 2010 Elsevier B.V.


Zou Y.,Sichuan University | Huang Z.,Sichuan University | Wang Y.,Sichuan University | Liao X.,Sichuan University | And 2 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2013

Co-doped ZnO particles were successfully prepared via a facile biomineralization process in the template of silk-fibroin (SF) peptides at room temperature, and SF peptides were coated onto the surface of particles. The ratio of Zn/Co in reactive solution could substantially influence the morphology of as-prepared particles, and the rough spherical particles including some nanoparticles of 50. nm diameters could be obtained at 4:1 ratio of Zn/Co. The saturation magnetization of SF-coated Co-ZnO particles was 8.63. emu/g, much larger than that of Co-ZnO without SF. L929 cell test revealed that the Co-doped ZnO particles had a good cellular compatibility at the concentration of less than 0.25. mg/mL due to silk-peptide coating, indicating that the prepared Co-doped ZnO particles have a potential for the biomedical applications. © 2012 Elsevier B.V.


Liu J.,Sichuan University | Deng M.,Sichuan University | Huang Z.,Sichuan University | Yin G.,Sichuan University | And 2 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2013

There is an urgency to prepare non-neurotoxic nanoparticles (NPs) as MRI contrast agent. We prepared ZnFe2O4 NPs with monocrystal structure in the template of silk-fibroin (SF) peptide at room temperature. Analyses of their crystallite size and NPs diameter after the growth of 1d and 8d indicated that SF peptide could promote nucleation, growth and dispersion of NPs. Results of EDS, Raman and TG proved that there was 4wt% of the chemical composition of SF in as-prepared NPs. A possible growth mechanism of ZnFe2O4 NPs in the template of SF is proposed. The saturation magnetizations of NPs with SF (SF-NPs) were about 15 and 30emu/g for 1 and 8 d, respectively. SF-NPs obviously promoted the viability of PC12 cells at NPs concentration of 0.0625mg/mL in 2d and 5d of co-culture. Analysis of neurite length indicated no significant decreases of the experimental groups at different SF-NPs concentrations (from 0.0625 to 0.5mg/mL) after 5d cell co-culture, because SF peptide coating could slow the release of iron/zinc ions from NPs. Furthermore, SF-NPs did not destroy the organelles, karyotheca and the neurite using the observation in the cell ultrathin sections. Based on their good magnetic property and neuro-cytocompatibility, the potential application of ZnFe2O4 NPs with SF as MRI contrast agents would be envisioned. © 2013 Elsevier B.V.

Loading The Military General Hospital of Chengdu PLA collaborators
Loading The Military General Hospital of Chengdu PLA collaborators