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Mohan Das N.,P.A. College | Sivakama Sundari S.,Arignar Anna College | Karuppusamy S.,The Madura College Autonomous | Mohan V.R.,Ethnopharmacology Unit | Parthipan B.,P.A. College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Objective: GC-MS (Gas chromatography and mass spectroscopy) analysis of ethanol extracts of leaf and stem bark of Cleidion nitidum was carried out to detect the bioactive components. Methods: The chemical compositions of the ethanolic extract of leaves and bark of Cleidion nitidum were investigated using Perkin - Elmer Gas Chromatography - Mass Spectra while mass spectra of the compounds found in the extract was matched with the National Institute of Standards of Technology (NIST) library. Results: Gas chromatography mass spectra (GC-MS) analysis revealed the presence of 16 compounds from leaves and 5 compounds from bark of the Cledion nitidum were identified. In GC-MS analysis, some of the phytocomponents screened were squalene, 3, 7, 11, 15-Tetramethyl-2, hexadecen-1-ol, Phytol and Vitamin E in leaves whereas, Thiophene-3-carbonitrite tetrahydro-4-oxo-, Dimethyl-2,6,10-dodecatrien-1-ol, D-Mannotetradecane-1,2,3,4,5-pentaol and Octanal 7-methoxy-3-7-dimethyl (5.13%) major compounds in the bark extract. Conclusion: These results indicate the ethanol extract of leaf and stem bark of Cleidion nitidum possesses potent anti-tumor, anticancer, cancer preventive, anti-inflammatory, antioxidant, antibacterial and pesticide effects so that it can be recommended as a plant of phytopharmaceutical importance.

Thenmozhi C.,The Madura College Autonomous | Vignesh V.,Bharathidasan University | Thirumurugan R.,Bharathidasan University | Arun S.,Alagappa University
Iranian Journal of Environmental Health Science and Engineering | Year: 2011

Malathion was used in vivo on fresh water fish Labeo rohita to study its toxicity. The acute toxicity tests were conducted during certain intervals in various concentrations (5, 10, 15, 20, 25 and 30 mg/L) of malathion. The physical and chemical analyses of water were carried out by following APHA methods. While treating with malathion, the percentage of fish mortality was assessed during 24, 48, 72 and 96 hours. The lethal and sub-lethal concentration of malathion were found to be LC100 (25 mg/L) and LC0 (5 mg/L), respectively. The antioxidant enzyme activity (Catalase 43.1±2.3, 16.5±0.57, 23.9±0.17 μ moles of phenol liberated/min/100mg protein and Glutathion-S-transferase (GST) 270.5±0.16, 143.2±1.03, 215.5±0.72 μ moles of phenol liberated/min/100mg protein), in the liver, muscle and gill, respectively increased during the accumulation of malathion, whereas it decreased (Catalase 17±1.44, 7.9±0.23, 10.7±0.69 μ moles of phenol liberated/min/100mg protein and GST 219.5±1.12, 108.1±0.34, 160.2±0.46 μ moles of phenol liberated/min/100mg protein) in the liver, muscle and gill respectively during depuration period. The effects of malathion resulted in the gradual decrease of nucleic acids, protein, free amino acids (FAA) and glycogen. During recovery period, the levels of biochemical components progressively increased indicating a probable recovery from the disruption of internal organ. Hence, the pesticide intoxication has made defective consequences in the normal metabolic pathways which led increasing the rate of morality in fish population.

Ananthaswamy V.,The Madura College Autonomous | Sahaya Amalraj L.,Raja Doraisingam Government Arts College
ARPN Journal of Engineering and Applied Sciences | Year: 2015

This paper demonstrates the effect of convective cooling on a temperature dependent viscosity liquid flowing steadily through a cylindrical pipe. In this model, it is assumed that due to Newton's cooling law heat is exchanged with the ambient and the viscosity model varies as an inverse linear function of temperature. The analytical expressions for fluid velocity and temperature are derived using Homotopy Analysis method and entropy generation rate, total entropy generated and the Bejan number for various parametric values are determined. Our results are compared with the previous work and found to be in good agreement. © 2006-2015 Asian Research Publishing Network (ARPN).

Muthukaruppan S.,District Institute of Education and Training | Senthamarai R.,SRM University | Rajendran L.,The Madura College Autonomous
International Journal of Electrochemical Science | Year: 2012

A mathematical modeling of immobilized glucoamylase kinetics by flow calorimetry is discussed. The model is based on non-stationary diffusion equation containing a non-linear term related to kinetics of the enzymatic reaction. This paper presents the complex numerical methods (The modified Adomian decomposition method) to solve the non-linear differential equations that describe the diffusion coupled with a non-linear reaction terms. Approximate analytical expressions for substrate concentration have been derived for all values of enzyme reactions parameters. © 2012 by ESG.

Senthilkumar K.P.,The Madura College Autonomous | Senthilkumar K.P.,Shree M And N Virani Science College | Thirumurugan R.,The Madura College Autonomous | Thirumurugan R.,Bharathidasan University
Asian Pacific Journal of Cancer Prevention | Year: 2012

Background: Tobacco contains agents which generate various potent DNA adducts that can cause gene mutations. Production of DNA adducts may be neutralized by glutathione S transferase (GST) along with other phase I and phase II enzyme systems. The existence of null type of GST among the population increases the susceptibility to various disorders and diseases. The present study focuses on the impact of high tobacco usage and possible null type mutation in GST loci. Methods: Genotypes of GST were detected by multiplex polymerase chain reaction in unrelated 504 volunteers of high tobacco using natives of Gujarat. Allelic frequencies were calculated using Statistical Package for Social Studies-16 software. Hardy Weinberg Equilibrium (HWE) was calculated using Chi square test. Two sided Fisher's significance test was used to compare allelic frequencies of different populations. Results: The frequency of homozygous null genotype of GSTM1 and GSTT1 were 20% (95% CI 16.7-23.9) and 35.5% (95% CI 31.4-39.9) respectively. The GSTM1 and GSTT1 null allele frequency distribution in the Gujarat population was significantly deviating from HWE. GSTT1 null frequency of Gujaratians was significantly higher and different to all reported low tobacco using Indian ethnics, while GSTM1 was not differing significantly. Conclusion: Tobacco usage significantly influences the rate of mutation and frequency of GSTT1 and M1 null types among the habituates. The rate of mutation in GSTT1 loci was an undeviating response to the dose of tobacco usage among the population. This mutational impact of tobacco on GSTT1 postulates the possible gene - environment interaction and selection of null genotype among the subjects to prone them under susceptible status for various cancers and even worst to cure the population with GSTT1 dependent drugs.

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