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Lee J.E.,Armed Forces Capital Hospital | Kim H.-J,The Korean Institute of Tuberculosis | Lee S.W.,Armed Forces Capital Hospital | Lee S.W.,Seoul National University
BMC Infectious Diseases | Year: 2011

Background: The roles of the tuberculin skin test (TST) and QuantiFERON®-TB Gold In-Tube assay (QFT-IT) in the diagnosis of active tuberculosis (TB) are not clear in young adults. We evaluated the diagnostic accuracy of the TST and QFT-IT in smear-negative TB among young adults with no underlying disease.Methods: We prospectively enrolled 166 young participants 20-29 years of age with suspected active TB in a military hospital of South Korea. The TST and QFT-IT were performed for all participants.Results: Of the 143 patients included in the analysis, active TB was diagnosed in 100 (69.9%). There were 141 male patients, none of whom had immunosuppressive disease. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of TST were 94% (95% CI, 87-98%), 88% (95% CI, 74-96%), 95% (95% CI, 88-98%), and 86% (95% CI, 72-94%), respectively. The sensitivity, specificity, PPV, and NPV of the QFT-IT were 93% (95% CI, 86-97%), 95% (95% CI, 81-99%), 98% (95% CI, 92-99%), and 84% (95% CI, 69-93%), respectively. No significant differences were found between the TST and QFT-IT in any statistic.Conclusions: Both the TST and QFT-IT showed high sensitivity and specificity in differentiating active TB from other diseases. The diagnostic accuracy of these two tests did not differ significantly when applied to this clinical population of young, immunocompetent adults in whom neonatal BCG vaccination was common, there was no history of previous TB and in whom suspicion of TB was high. Trial registration: ClinicalTrials.gov: NCT00982969. © 2011 Lee et al; licensee BioMed Central Ltd.

Song K.-S.,Biometrix Technology Inc. | Nimse S.B.,Hallym University | Kim H.J.,The Korean Institute of Tuberculosis | Yang J.,The Korean Institute of Tuberculosis | Kim T.,Hallym University
Sensors (Switzerland) | Year: 2016

In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%). © 2016 by the authors; licensee MDPI, Basel, Switzerland.

Kim B.-J.,Seoul National University | Hong S.-H.,Seoul National University | Yu H.-K.,The Korean Institute of Tuberculosis | Park Y.-G.,The Korean Institute of Tuberculosis | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A previously undescribed, slowly growing, non-chromogenic Mycobacterium strain (299T) was isolated from the sputum sample of a patient with a symptomatic pulmonary infection. Phenotypically, strain 299T was generally similar to Mycobacterium koreense DSM 45576T and Mycobacterium triviale ATCC 23292T. The 16S rRNA gene sequence of strain 299T was similar to that of M. koreense DSM 45576T (GenBank accession no. AY734996, 99.5% similarity); however, it differed substantially from that of M. triviale ATCC 23292T (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 299T clustered together with M. koreense DSM 45576T and M. triviale ATCC 23292T, supported by high bootstrapping values (99 %). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, the hsp65 and rpoB genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 299T represents a novel mycobacterial species, for which the name Mycobacterium parakoreense sp. nov. is proposed. The type strain is 299T (=DSM 45575T=KCTC 19818T). © 2013 IUMS.

Kim B.-J.,Seoul National University | Math R.K.,Chung - Ang University | Jeon C.O.,Chung - Ang University | Yu H.-K.,The Korean Institute of Tuberculosis | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A slow-growing non-chromogenic mycobacterium was isolated from a patient with pulmonary disease. Phenotypically, strain 05-1390T was similar to Mycobacterium intracellulare ATCC 13950T. The 16S rRNA gene sequence (1385 bp) of strain 05-1390T showed a high degree of similarity to those of the M. intracellulare complex, namely Mycobacterium marseillense 5351974T (100%), M. intracellulare ATCC 13950T (99.8%) and Mycobacterium chimaera DSM 44623T (99.9%). Phylogenetic analysis based on internal transcribed spacer 1 (ITS1) and the hsp65 gene indicated that strain 05-1390T was closely related to M. intracellulare ATCC 13950T, but that it was a distinct phylogenetic entity. Of particular interest, an analysis based on the rpoB gene (701 bp) showed that it is closely related to Mycobacterium parascrofulaceum ATCC BAA-614T (99.4%), a scotochromogenic strain, rather than to the M. intracellulare-related strains. Unique MALDI-TOF MS profiles also supported the taxonomic status of this strain as a distinct species. These data support the conclusion that strain 05-1390T represents a novel mycobacterial species, for which the name Mycobacterium yongonense sp. nov. is proposed; the type strain is 05-1390T (5DSM 45126T5KCTC 19555T). © 2013 IUMS.

Ryoo S.,The Korean Institute of Tuberculosis | Kim H.J.,The Korean Institute of Tuberculosis
Osong Public Health and Research Perspectives | Year: 2014

The Korean National Tuberculosis Association (KNTA) set up the Korean Institute of Tuberculosis (KIT) in 1970 to foster research and technical activities pertaining to tuberculosis (TB). The KNTA/KIT had successfully conducted a countrywide TB prevalence survey from 1965 to 1995 at 5-year intervals. The survey results (decline in TB rates) established Korea as a country that had successfully implemented national control programs for TB. The KIT developed the Korea Tuberculosis Surveillance System and the Laboratory Management Information System, both of which were transferred to the Korea Centers for Disease Control and Prevention after its establishment. The KIT functions as a central and supranational reference TB laboratory for microbiological and epidemiological research and provides training and education for health-care workers and medical practitioners. Recently, the KIT has expanded its activities to countries such as Ethiopia, Laos, and Timor-Leste to support TB control and prevention. The KIT will continue to support research activities and provide technical assistance in diagnosing the infection until it is completely eliminated in Korea. © 2014.

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