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Godlewski M.M.,Macquarie University | Godlewski M.M.,Warsaw University of Life Sciences | Pietrzak P.,Warsaw University of Life Sciences | Kotunia A.,The Kielanowski Institute of Animal Physiology and Nutrition | And 2 more authors.
Livestock Science | Year: 2010

A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor β1 (TGF-β1) were overlayed with electron microscopy finder-grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF-β1-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine. © 2010 Elsevier B.V. Source


Jurka P.,Warsaw University of Life Sciences | Max A.,Warsaw University of Life Sciences | Hawrynska K.,Warsaw University of Life Sciences | Snochowski M.,The Kielanowski Institute of Animal Physiology and Nutrition
Reproduction in Domestic Animals | Year: 2010

Contents: The cystic endometrial hyperplasia and pyometra complex is one of the most common uterine diseases in bitches. The appearance of pharmacological preparations containing anti-progestagens created new possibilities for pyometra treatment. The aim of this study was to evaluate the curative effect of the anti-progestagen aglepristone treatment of pyometra in bitches of different ages. Twenty four bitches of different breeds, aged from 0.8 to 9.5 years (21-48 kg) exhibiting clinical pyometra symptoms (two groups - I ≤ 5 years, n = 14 and II >5 years, n = 10) were evaluated. Information about the general reproductive health was collected up to 54 months after anti-progestagen treatment. Remission of clinical symptoms and return of blood chemistry results and total leucocyte count to referential values were achieved in all cases within 14 days of treatment. Bitches were naturally mated at the first, and when unsuccessful, the second oestrus after treatment. In group I, no recurrence of pyometra symptoms was observed during following cycle(s). Eight bitches (57.1%) had a full-term pregnancy and the number of newborn pups ranged from 1 to 12. None of the bitches from the group II became pregnant. In conclusion, the basic indication for conservative pharmacological treatment of pyometra is preserving female fertility and obtaining offspring. The important conditions for successful aglepristone treatment are: the young age (up to 5 years) and the lack of detectible ovarian cysts. It seems necessary to mate bitches in the first or second oestrus after finishing treatment. The efficacy of treatment can be measured by the after-treatment pregnancy rate. © 2008 The Authors. Journal compilation © 2008 Blackwell Verlag. Source


Zielinska M.,Warsaw University of Life Sciences | Zielinska M.,The Kielanowski Institute of Animal Physiology and Nutrition | Sawosz E.,Warsaw University of Life Sciences | Grodzik M.,Warsaw University of Life Sciences | And 5 more authors.
Archives of Animal Nutrition | Year: 2012

The objective of the present investigation was to evaluate the effects of taurine and Au nanoparticles on the expression of genes related to embryonic muscle development and on the morphological characteristics of muscles. Fertilised chicken eggs (n = 160) were randomly divided into four groups: without injection (Control) and with injection of Au nanoparticles (NanoAu), taurine (Tau) or Au nanoparticles with taurine (NanoAu + Tau). The experimental solutions were given in ovo, on the third day of incubation, by injecting 0.3 ml of the experimental solution into the air sack. The embryos were evaluated on the 20th day of incubation. The methods included gene expression at the mRNA and protein levels, immunohistochemistry, histology and microscopy. In groups NanoAu, Tau and NanoAu + Tau, the muscle structure and the number of muscle cells were affected. Furthermore, taurine increased fibre diameter, the total number of nuclei, the proportion of proliferating cell nuclear antigen (PCNA)-positive cells and the total cell number. Also, gene expression of basic fibroblast growth factor-2 and PCNA was downregulated. There were no significant interactions between NanoAu and taurine, indicating that NanoAu did not enhance the effects of taurine. It may be concluded that 20 days after injection, NanoAu affected some parameters of muscle development, but the most profound effects were those of taurine. © 2012 Taylor and Francis Group, LLC. Source

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