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Lu W.,Chinese Academy of Agricultural Sciences | Lu W.,Dalian University of Technology | Zhang Z.,Chinese Academy of Agricultural Sciences | Xu P.,Dalian University of Technology | And 4 more authors.
Journal of Phytopathology | Year: 2012

We have developed a multiplex RT-PCR protocol for the simultaneous detection of three viroids in three different genera that infect hops: Hop latent viroid (HLVd; Cocadviroid), Hop stunt viroid (HSVd; Hostuviroid) and Apple fruit crinkle viroid (AFCVd; Apscaviroid). The method was validated by testing 175 hop samples collected from the Xinjiang autonomous region of China. All samples were found to be positive for HLVd but negative for AFCVd, confirming the widespread or even ubiquitous infection of HLVd and the low incidence of AFCVd in hops in China. In addition, HSVd was detected in 22.86% of the samples tested. This rapid and reliable multiplex RT-PCR assay provides an effective method for detection of three important viroid species in large-scale surveys for disease management in hops. © 2012 Blackwell Verlag GmbH. Source

Hua F.A.N.,Shihezi University | Hua F.A.N.,The Key Oasis Eco Agriculture Laboratory of Xinjiang Production and Construction Group | Feng L.,Shihezi University | Feng L.,The Key Oasis Eco Agriculture Laboratory of Xinjiang Production and Construction Group | And 4 more authors.
Research on Crops | Year: 2013

Drip irrigation has been applied to sugarbeet cultivation in Xinjiang currently, while there are presently very few reports concerning optimal irrigation amounts and nitrogen application quantities in the cultivation of drip irrigated sugarbeet in Xinjiang, China. This study provides a theoretical basis for economically rational irrigation and nitrogen application programmes enabling the standardized cultivation of sugarbeet. Field tests were performed in conjunction with a two-factor quadratic saturation D optimum design scheme involving moisture and nitrogen to establish a binary quadratic polynomial mathematical model with moisture and nitrogen as independent variables and root yield and sugar concentration as target functions. Analysis and optimization using the model yielded the optimal irrigation and nitrogen application amounts. Results showed that there was a significant interaction between moisture and nitrogen with regard to root yield and sucrose content. Moisture is the chief factor affecting beet root weight and sugar content, followed by nitrogen. Moisture and nitrogen have a significant interactive promoting effect on root yield, but also have a significant interactive inhibiting effect on sucrose concentration. Under low levels of irrigation and nitrogen application, root yield increases with irrigation amount and the nitrogen application rate. However, after certain levels are reached, increases in root yield are not significant. Sucrose content first decreases then increases with irrigation amount and nitrogen application rate. Under the experimental conditions employed in this study, irrigation amounts and nitrogen application rates of 6417-8100 m3/ha and 166.5-292.8 kg/ha, respectively, achieved root yields exceeding 80 t/ha. Source

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