Wang H.-Y.,South China University of Technology |
Jia L.-H.,South China University of Technology |
Wu R.-R.,South China Institute of Environmental Sciences |
Wu R.-R.,The Key Laboratory of Water and Air Pollution Control of Guangdong Province |
And 2 more authors.
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2014
A total of 33 fecal samples of human and different animal species were collected in the Pearl River Delta region. Genomic DNA were then efficiently extracted and amplified by using 14 specific Bacteroidales primers that have been reported as tracking markers of different sources. Primers with high specificity and sensitivity were then selected and used for polluted water samples analysis. The results show that the purity and extract efficiency of the genome DNA meet the requirement of the following experiments. 85% of the 33 fecal samples analyzed were positive when using Bacteroidales universal primer 2#(Bac32F/Bac708R), and most of the mammalian animals and chicken fecal samples were detected as positive samples when use this primer. Human-specific marker 3#(HF134F/Bac708R) and 4#(HF183F/Bac708R), ruminant-specific marker 8#(CF128F/Bac708R) and porcine-specific marker 10#(PF163F/Bac708R) showed high sensitivity and specificity in the Pearl River Delta region. Polluted water samples analysis results were the same as the actual type of pollution, implying Bacteroidales universal primer 2#, human-specific primer 3# and 4# and porcine-specific primer 10# could be used as pollution tracking markers in the Pearl River Delta region.
Dai Y.-L.,Appraisal Center for Environment and Engineering |
Tao J.,South China Institute of Environmental Sciences |
Tao J.,The Key Laboratory of Water and Air Pollution Control of Guangdong Province |
Lin Z.-J.,South China Institute of Environmental Sciences |
And 3 more authors.
Huanjing Kexue/Environmental Science | Year: 2013
The meteorological and environmental data including visibility, SO2, NO2 and PM10 were collected in four major megacities (Beijing, Shanghai, Guangzhou and Chengdu) in the years 2006-2009. Based on the data, seasonal and annual variations of the haze frequency and the key impact factors were discussed. The results indicated that the highest frequencies of haze occurred in summer, winter, spring and autumn for Beijing, Shanghai, Guangzhou and Chengdu, respectively. The trends of haze frequency decreased in Beijing and Guangzhou, while increased in Shanghai and Chengdu during the studied period. The PM10 concentration and relative humidity were the key factors for visibility degradation in the four megacities. The variation of visibility was sensitive to relative humidity in Beijing, to PM10 in Shanghai and Guangzhou, and to both in Chengdu.
Lu J.,South China University of Technology |
Guo C.,South China University of Technology |
Guo C.,The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters |
Li J.,South China University of Technology |
And 6 more authors.
World Journal of Microbiology and Biotechnology | Year: 2013
A fusant strain F14 with high biodegradation capability of phenanthrene was obtained by protoplast fusion between Sphingomonas sp. GY2B (GenBank DQ139343) and Pseudomonas sp. GP3A (GenBank EU233280). F14 was screened and identified from 39 random fusants by antibiotic tests, scanning electron microscope (SEM) and randomly amplified polymorphic DNA (RAPD). The result of SEM analysis demonstrated that the cell shape of fusant F14 different from parental strains. RAPD analysis of 5 primers generated a total of 70 bands. The genetic similarity indices between F14 and parental strains GY2B and GP3A were 27.9 and 34.6 %, respectively. F14 could rapidly degrade phenanthrene within 24 h, and the degradation efficiency was much better than GY2B and GP3A. GC-MS analysis of metabolites of phenanthrene degradation indicated F14 had a different degradation pathway from GY2B. Furthermore, the fusant strain F14 had a wider adaptation of temperatures (25-36 °C) and pH values (6.5-9.0) than GY2B. The present study indicated that fusant strain F14 could be an effective and environment-friendly bacterial strain for PAHs bioremediation. © 2013 Springer Science+Business Media Dordrecht.
Jiang D.,South China Institute of Environmental Sciences |
Jiang D.,The Key Laboratory of Water and Air Pollution Control of Guangdong Province |
Jiang D.,State Environmental Protection Key Laboratory of Water Environmental Simulation and Pollution Control |
Liu Y.,South China Institute of Environmental Sciences |
And 7 more authors.
Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae | Year: 2013
A novel electromagnetic aerobic membrane bioreactor (EMAMBR) was developed to remove trace naphthalene(Nap) and phenanthrene(Phe) in effluent from a printing and dyeing wastewater treatment plant. The performance of the EMAMBR was evaluated by monitoring the degradation efficiency of Nap and Phe compared to conventional aerobic MBR and electro-aerobic MBR. The effects of some parameters such as current strength, hydraulic retention time (HRT) and initial pH on the performance of the EMAMBR were investigated and degradation pathways of the Nap and Phe were proposed based on GC/MS data. The results showed that the EMAMBR could achieve enhanced degradation of Nap and Phe compared with conventional aerobic MBR and electro-aerobic MBR. The average degradation efficiency of Nap and Phe in the EMAMBR were 30%~60% higher than that obtained from the conventional aerobic MBR and electro-aerobic MBR and was improved with the increase of current strength and HRT. Up to 75% Nap(2.77~4.75 μg·L-1 )and Phe (2.0~8.0 μg·L-1)were degraded at current strength 20 mA, HRT 4 h and pH 6.5~7.5 and the total concentration of Polycyclic Aromatic Hydrocarbons (PAH) in effluent can meet the national drinking water standards(GB 5749-2006). The Nap was degraded via the salicylic acid pathway while Phe was degraded via both salicylic acid and o-phthalic acid pathways.
Tang Z.,South China Institute of Environmental Sciences |
Tang Z.,The Key Laboratory of Water and Air Pollution Control of Guangdong Province |
Cen C.,South China Institute of Environmental Sciences |
Cen C.,The Key Laboratory of Water and Air Pollution Control of Guangdong Province |
And 6 more authors.
Chinese Journal of Environmental Engineering | Year: 2015
In China, the flat glass industry is facing great challenge in NOx control. The research and application of highly efficient deNOx technologies for flue gas from flat glass furnaces are benefit to both glass industry development and atmospheric environmental protection. Selective catalytic reduction (SCR) of NOx is considered to be the most efficient deNOx technology, but some technical problems prevent SCR technology from glass furnaces. On the basis of the successful design and construction of pilot scale (10 000 m3/h) SCR system at a flat glass furnace, we analyzed the influent fluctuation and the corresponding effects, investigated the critical process parameters including flue gas temperature, ammonia control and flue gas volume, and then carried out a six-day running test. By these work conducted in real flue gas, a number of first-hand data and experience focusing on SCR technology for the deNOx of flat glass furnaces were shared in this paper. ©, 2015, Science Press. All right reserved.