Institute of the Cancer Research

Fort Worth, TX, United States

Institute of the Cancer Research

Fort Worth, TX, United States

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Mukerjee A.,Institute of the Cancer Research | Luchowski R.,Fort Technologies | Luchowski R.,Maria Curie Sklodowska University | Ranjan A.P.,Institute of the Cancer Research | And 4 more authors.
Current Pharmaceutical Biotechnology | Year: 2010

We report a significant increase of a curcumin (1,7-bis[4-hydroxy-3- methoxyphenyl]-1,6-heptadiene-3,5-dione) fluorescence brightness when deposited on plasmonic platforms (self assembled silver nanostructures formed on the surface of silver semitransparent film). The enhancement of fluorescence intensity is accompanied by a strong decrease in fluorescence lifetime. Simultaneously, the increased photostability of curcumin, a pigment showing strong antiinflammatory, antioxidant and antitumorigenic activity, allows long-time detection and monitoring. We believe that the use of plasmonic platforms will improve detection, delivery and imaging of curcumin. © 2010 Bentham Science Publishers Ltd.


Fudala R.,University of North Texas Health Science Center | Fudala R.,Jan Kochanowski University | Ranjan A.P.,Institute of the Cancer Research | Mukerjee A.,Institute of the Cancer Research | And 6 more authors.
Current Pharmaceutical Biotechnology | Year: 2011

MMP-9 enzyme recognizes a peptide sequence Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys and cleaves the peptide into two parts. We synthesized a dual fluorophore beacon consisting of 5-FAM and Cy5 dyes. The fluorescence emission of the fluorescein moiety is dramatically quenched by Cy5 molecule due to Förster Resonance Energy Transfer (FRET) and the fluorescence of Cy5 is strongly enhanced. Upon addition of MMP-9 enzyme, the fluorescence of 5-FAM intensifies and Cy5 decreases. The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions. © 2011 Bentham Science Publishers Ltd.

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