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Liu X.-H.,The Institute of Blood Transfusion | Chi X.-Y.,The Institute of Blood Transfusion | Jiao S.-X.,The Institute of Blood Transfusion
Chinese Journal of Tissue Engineering Research

BACKGROUND: The discovery of novel HLA alleles is accelerated by development of molecular biology and applications of numerous new technologies. These new findings not only rich HLA family, but also find a breakthrough point for the study of genetic superiority or disappearance of national gene. OBJECTIVE: To identify two novel HLA-A alleles and to analysis their nucleotide sequences. METHODS: The two samples from two volunteers of Chinese Marrow Donor Program were detected using PCR-SBT and GSSP methods. The HLA-A locus in the two samples were both abnormal genes, and the nucleotide sequence differences were analyzed. RESULTS AND CONCLUSION: The sequences of the samples were different from all alleles in the HLA databases. Sample 1 was found to be different from the closet matching allele A*24:02:01 in one nucleotide substitutions, 360 G > C in exon 3, resulting in amino acid changed from glutamine to histidine at codon 96; and sample 2 differed from A*26:01:01 in one nucleotide substitution, 97 T > C in exon 2, resulting in amino acid changed from tyrosine to histidine at codon 9. The novel alleles were identified and assigned the name HLA-A*24:233 and HLA-A*26:89 officially by the WHO Nomenclature Committee. © 2015 Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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