The Inner Mongolia Autonomous Region Peoples Hospital

Hohhot, China

The Inner Mongolia Autonomous Region Peoples Hospital

Hohhot, China
SEARCH FILTERS
Time filter
Source Type

Zhang S.,Chongqing Key Laboratory of Neurobiology | Zhang S.,Chongqing Medical University | Wang W.,Chongqing Medical University | Wang W.,Chongqing Key Laboratory of Neurobiology | And 16 more authors.
Behavioural Brain Research | Year: 2016

CD36 is a member of the class B scavenger receptor family of cell surface proteins, which plays a major role in fatty acid, glucose and lipid metabolism. Besides, CD36 functions as a microglial surface receptor for amyloid beta peptide. Regarding this, we suggest CD36 might also contribute to neuropsychiatric disease. The aim of this study was to achieve a behavioral phenotype of CD36 knockout (CD36-/-) mice. We characterized the behavior of CD36-/- mice and C57BL/6J mice by subjecting them to a series of tests, which include SHIRPA primary behavioral screen test, 1% sucrose preference test, elevated plus-maze test, open-field test and forced swimming test. The results showed that CD36-/- mice traversed more squares, emitted more defecation, exhibited higher tail elevation and had more aggressive behaviors than C57BL/6J mice. The CD36-/- mice spent more time and traveled longer distance in periphery zone in the open-field test. Meanwhile, the numbers that CD36-/- mice entered in the open arms of elevated plus-maze were reduced. These findings suggest that CD36-/- mice present an anxious phenotype and might be involved in neuropsychiatric disorders. © 2015.


Wu Y.,Chongqing Medical University | Wu Y.,Chongqing Key Laboratory of Neurobiology | Fu Y.,Chongqing Key Laboratory of Neurobiology | Fu Y.,Chongqing Medical University | And 21 more authors.
Behavioural Brain Research | Year: 2016

Major depressive disorder (MDD) is a debilitating illness. However, the underlying molecular mechanisms of depression remain largely unknown. Increasing evidence supports that inflammatory cytokine disturbances may be associated with the pathophysiology of depression in humans. Systemic administration of lipopolysaccharide (LPS) has been used to study inflammation-associated neurobehavioral changes in rodents, but no metabonomic study has been conducted to assess differential metabolites in the prefrontal cortex (PFC) of a LPS-induced mouse model of depression. Here, we employed a gas chromatography-mass spectrometry-based metabonomic approach in the LPS-induced mouse model of depression to investigate any significant metabolic changes in the PFC. Multivariate statistical analysis, including principal component analysis (PCA), partial least squares-discriminate analysis (PLS-DA), and pair-wise orthogonal projections to latent structures discriminant analysis (OPLS-DA), was implemented to identify differential PFC metabolites between LPS-induced depressed mice and healthy controls. A total of 20 differential metabolites were identified. Compared with control mice, LPS-treated mice were characterized by six lower level metabolites and 14 higher level metabolites. These molecular changes were closely related to perturbations in neurotransmitter metabolism, energy metabolism, oxidative stress, and lipid metabolism, which might be evolved in the pathogenesis of MDD. These findings provide insight into the pathophysiological mechanisms underlying MDD and could be of valuable assistance in the clinical diagnosis of MDD. © 2016 Elsevier B.V.


Cao Z.,Breast | Zeng Q.,The Inner Mongolia Autonomous Region Peoples Hospital | Pei H.,Breast
Oncology Letters | Year: 2016

It is well known that heat shock protein 90 (HSP90) overexpression is correlated with poor prognosis and chemo‑resistance in human malignant cancers. At the same time, wighteone, or 6‑prenyl‑5,7,4'‑trihydroxyisoflavone, a major isoflavone component of the ornamental tall tree Erythrina suberosa, has been demonstrated to exhibit a potent anti‑proliferative effect on human leukemia HL‑60 cancer cell lines. In this study, the effects of wighteone on the proliferation of HER2‑positive breast cancer cells were investigated, and the action mechanism was explored. MCF‑7 HER2‑positive breast cancer cells were treated with various concentrations of wigh­teone. The growth inhibitory rate of the cells was calculated by MTT assay, apoptosis was detected by flow cytometry, and the expression level of HSP90 was assessed by western blot analysis. The addition of wighteone at concentrations ranging from 1‑10 g/ml in the medium for 48 h had a marked inhibi­tion on the proliferation of HER2‑positive cancer cell lines. The growth inhibitory rates with 0.5, 2 or 8 mM wighteone were significantly higher compared with the control group. Apoptosis in the wighteone‑treated cells was also significantly higher compared with the control group. The expression level of HSP90 in the wighteone group was significantly lower than that in the control group. Our findings demonstrated that wigh­teone effectively inhibited the proliferation of HER2‑positive cancer cell lines, and this is considered to be the result of downregulating HSP90 receptor and downstream signaling. © 2016, Oncology Letters. All rights reserved.


Sun L.-T.,Inner Mongolia Medical College | Xiao W.-L.,The Inner Mongolia Autonomous Region Peoples Hospital
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2015

OBJECTIVE: To study the expression level of p53 in tumor tissue of patients with acute leukemia (AL) and its clinical significance.METHODS: From April 2013 to April 2015, 80 patients with AL in our hospital were chosen as leukemia group, at the same time, 50 patients with non-hematologic diseases were chosen as control group. All the patients were detected by bone marrow smear mean and p53 staining. The positive rate of p53 and staining score were compared between 2 groups. The clinical data of leukemia group were collected, and the correlation of clinical features with p53 expression was analyzed.RESULTS: In the control group, the positive rate of p53 was 6.00%, the staining score was (0.2 ± 0.1); in the leukemia group, the positive rate of p53 was 73.75%, the staining score was (1.9 ± 0.4), the difference was statistically significant (P < 0.05). The expression of p53 significantly correlated with the blood routine indicators, clinical manifestation, multiple infiltration and curative effects (P < 0.05), and the p53 expression not correlated statistically with the sex, age, anamnesis, family history (P > 0.05).CONCLUSION: Compared with the patients with non-hematologic diseases, the expression level of p53 in the AL patients increases significantly, the p53 expression correlates significantly with the blood routine indicators, clinical manifestation and curative effect of the patients.


Lin X.,Chongqing Medical University | Zhou X.,Chongqing Medical University | Liu D.,Chongqing Medical University | Yun L.,The Inner Mongolia Autonomous Region Peoples Hospital | And 4 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2016

Hyperglycemia or high-glucose (HG)-induced apoptosis in human retinal pigment epithelial (RPE) cells is a characteristic process in diabetic retinopathy. In our study, we examined whether microRNA-29 (miR-29) may regulate HG-induced RPE cell apoptosis. Human RPE cell line, ARPE-19 cells, was treated with various high concentration of glucose in vitro. HG-induced RPE cell apoptosis was examined by terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay and miR-29 gene expression by quantitative RT-PCR (qRT-PCR). miR-29 was then downregulated in RPE cells, and its effect on HG-induced apoptosis was examined by TUNEL assay and western blot assay on caspase-7 protein. Association of miR-29 on its downstream target, PTEN, in HG-induced RPE cell apoptosis was evaluated by dual-luciferase assay and qRT-PCR. PTEN was silenced in RPE cells. The effects of PTEN downregulation on miR-29-mediated HG-induced RPE cell apoptosis were also examined by TUNEL and western blot assays. HG induced significant apoptosis in RPE cells in a dose-dependent manner. miR-29 was upregulated by HG in RPE cells. miR-29 downregulation protected HG-induced apoptosis and reduced the production of caspase-7 protein in RPE cells. PTEN was shown to be directly downregulated by HG and then upregulated by miR-29 downregulation in RPE cells. Small interfering RNA (siRNA)-mediated PTEN downregulation reversed the protective effect of miR-29 downregulation on HG-induced RPE cell apoptosis. This study demonstrates that miR-29, through inverse association of PTEN, plays an important role in the process of HG-induced apoptosis in RPE cells. © 2016 The Society for In Vitro Biology


Jia Y.,Inner Mongolia University | Wu D.,Erdos City Central Hospital | Yun F.,Inner Mongolia University | Shi L.,Inner Mongolia University | And 7 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Epithelial-mesenchymal transition (EMT) is associated with altered connection and junctions between cells and changes in abilities of invasion and migration. In this study, we investigated whether SK-BR-3 breast cancer cells induced to undergo EMT exhibit changes in morphological and invasion abilities after Transforming growth factor β1 (TGF-β1) treatment. Serum-deprived SK-BR-3 cells were treated with TGF-β1 (0, 10 ng/mL) for 24 h. The cells morphological changes were observed and imaged using inverted phase contrast microscope. Scratch experiment and invasion experiment were employed to detect changes of invasion ability, cell-flow experiment was used to assess cell cycle, immunohistochemistry technique was used to detect epithelial and mesenchymal markers after the crawling cells were fixed. Our research reveal that SK-BR-3 cells become larger and more messy, the elongated cells extend pseudopodia, the link of the cells became more loosely and cell gap widened after TGF-β1 treatment. SK-BR-3 cells showed faster growing and improved invasion abilities after TGF-β1 treatment, and reduced G1 phase cells proportion in the total number of cells after the conversion, in contrast the S phase cells accounted for the proportion of the total number of cells increased. These findings indicate that TGF-β1-induced EMT in breast cancer cells may be associated with major alterations in morphological and invasion abilities.


PubMed | The Inner Mongolia Autonomous Region Peoples Hospital and Chongqing Medical University
Type: Journal Article | Journal: In vitro cellular & developmental biology. Animal | Year: 2016

Hyperglycemia or high-glucose (HG)-induced apoptosis in human retinal pigment epithelial (RPE) cells is a characteristic process in diabetic retinopathy. In our study, we examined whether microRNA-29 (miR-29) may regulate HG-induced RPE cell apoptosis. Human RPE cell line, ARPE-19 cells, was treated with various high concentration of glucose in vitro. HG-induced RPE cell apoptosis was examined by terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay and miR-29 gene expression by quantitative RT-PCR (qRT-PCR). miR-29 was then downregulated in RPE cells, and its effect on HG-induced apoptosis was examined by TUNEL assay and western blot assay on caspase-7 protein. Association of miR-29 on its downstream target, PTEN, in HG-induced RPE cell apoptosis was evaluated by dual-luciferase assay and qRT-PCR. PTEN was silenced in RPE cells. The effects of PTEN downregulation on miR-29-mediated HG-induced RPE cell apoptosis were also examined by TUNEL and western blot assays. HG induced significant apoptosis in RPE cells in a dose-dependent manner. miR-29 was upregulated by HG in RPE cells. miR-29 downregulation protected HG-induced apoptosis and reduced the production of caspase-7 protein in RPE cells. PTEN was shown to be directly downregulated by HG and then upregulated by miR-29 downregulation in RPE cells. Small interfering RNA (siRNA)-mediated PTEN downregulation reversed the protective effect of miR-29 downregulation on HG-induced RPE cell apoptosis. This study demonstrates that miR-29, through inverse association of PTEN, plays an important role in the process of HG-induced apoptosis in RPE cells.


PubMed | The Inner Mongolia Autonomous Region Peoples Hospital and Inner Mongolia Medical College
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2015

To study the expression level of p53 in tumor tissue of patients with acute leukemia (AL) and its clinical significance.From April 2013 to April 2015, 80 patients with AL in our hospital were chosen as leukemia group, at the same time, 50 patients with non-hematologic diseases were chosen as control group. All the patients were detected by bone marrow smear mean and p53 staining. The positive rate of p53 and staining score were compared between 2 groups. The clinical data of leukemia group were collected, and the correlation of clinical features with p53 expression was analyzed.In the control group, the positive rate of p53 was 6.00%, the staining score was (0.2 0.1); in the leukemia group, the positive rate of p53 was 73.75%, the staining score was (1.9 0.4), the difference was statistically significant (P < 0.05). The expression of p53 significantly correlated with the blood routine indicators, clinical manifestation, multiple infiltration and curative effects (P < 0.05), and the p53 expression not correlated statistically with the sex, age, anamnesis, family history (P > 0.05).Compared with the patients with non-hematologic diseases, the expression level of p53 in the AL patients increases significantly, the p53 expression correlates significantly with the blood routine indicators, clinical manifestation and curative effect of the patients.


PubMed | The Inner Mongolia Autonomous Region Peoples Hospital
Type: Journal Article | Journal: Oncology letters | Year: 2016

It is well known that heat shock protein 90 (HSP90) overexpression is correlated with poor prognosis and chemo-resistance in human malignant cancers. At the same time, wighteone, or 6-prenyl-5,7,4-trihydroxyisoflavone, a major isoflavone component of the ornamental tall tree

Loading The Inner Mongolia Autonomous Region Peoples Hospital collaborators
Loading The Inner Mongolia Autonomous Region Peoples Hospital collaborators