Cluj-Napoca, Romania
Cluj-Napoca, Romania

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Tamokou J.D.,Babes - Bolyai University | Tamokou J.D.,University of Dschang | Chouna J.R.,Babes - Bolyai University | Chouna J.R.,University of Dschang | And 11 more authors.
PLoS ONE | Year: 2013

Traditional remedies have a long-standing history in Cameroon and continue to provide useful and applicable tools for treating ailments. Here, the anticancer, antimicrobial and antioxidant activities of ten antioxidant-rich Cameroonian medicinal plants and of some of their isolated compounds are evaluated.The plant extracts were prepared by maceration in organic solvents. Fractionation of plant extract was performed by column chromatography and the structures of isolated compounds (emodin, 3-geranyloxyemodin, 2-geranylemodin) were confirmed spectroscopically. The antioxidant activity (AOA) was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) bleaching method, the trolox equivalent antioxidant capacity (TEAC), and the hemoglobin ascorbate peroxidase activity inhibition (HAPX) assays. The anticancer activity was evaluated against A431 squamous epidermal carcinoma, WM35 melanoma, A2780 ovary carcinoma and cisplatin-resistant A2780cis cells, using a direct colorimetric assay. The total phenolic content in the extracts was determined spectrophotometrically by the Folin-Ciocalteu method. Rumex abyssinicus showed the best AOA among the three assays employed. The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods. The lowest IC50 values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia. The Ficus asperifolia and Psorospermum febrifugum extracts are selective against A2780cis ovary cells, a cell line which is resistant to the standard anticancer drug cisplatin. Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin. Its selectivity against the platinum-resistant A2780cis cell line is highest. All of the extracts display antimicrobial activity, in some cases comparable to that of gentamycin. © 2013 Tamokou et al.


Muresan B.,Babes - Bolyai University | Cimpoiu C.,Babes - Bolyai University | Hosu A.,Babes - Bolyai University | Bischin C.,Babes - Bolyai University | And 4 more authors.
Studia Universitatis Babes-Bolyai Chemia | Year: 2015

Reported here is a first detailed analysis of the antioxidant activity in traditional fruit-derived distilled alcoholic beverages from Romania (“ţuica”). A distinctly highest activity, paralleled by Folin-Ciocalteu data and UV-vis absorbances, was seen in the most common type of such brandy, made of plums; other samples included brandies made of apples, pears, fruit mixtures, wine, and cereals. In fact, the values seen for the plum brandy were, even before maturation with wood, similar to those of commercially-available wood-maturated whisky. Increases of up to one order of magnitude in antioxidant activity were seen in all brandies upon maturation with various types of wood – with mulberry tree by far the most efficient, followed by oak and cherry – and with lowest values seen for acacia. Attempts to identify anticancer activity in concentrated extracts prepared from plum brandy, failed. Copper electron paramagnetic (EPR) signals are shown for the first time in such brandy samples. © 2015, Universitatea Babes-Bolyai, Catedra de Filosofie Sistematica. All rights reserved.

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