Hou J.,The General Hospital of Shenyang Military Region |
Wang H.,The General Hospital of Shenyang Military Region |
Li X.,The General Hospital of Shenyang Military Region |
Zhu Y.,The General Hospital of Shenyang Military Region
Experimental and Therapeutic Medicine | Year: 2017
The present study aimed to investigate the effects of remifentanil during adaptation followinsg myocardial ischemia, and its possible clinical applications. Remifentanil was used during the simulation of adaptation following ischemia, which was performed using a Langendorff heart perfusion system. A total of 75 rats were divided into five groups, and the coronary flow, cardiac output and the cardiac enzyme content in coronary effluent prior to ischemia and post-reperfusion were recorded. Electron microscopy was used to observe myocardial ultrastructure, and the volume of aortic and coronary effluent was also measured. The recovery of cardiac output upon reperfusion was significantly higher following remifentanil treatment (91%), as compared with the ischemic control group (78%; P<0.05). The coronary flow of the experimental group following reperfusion decreased by 4 ml/min compared with the control group not exposed to ischemia, whilst the coronary flow of the ischemic control group was reduced by 20-24 ml/min. Flameng scoring of the mitochondria demonstrated improved mitochondrial ultrastructure following remifentanil treatment (score, 1.25±0.31), as compared the ischemic control group (score, 3.14±0.17). Lactate dehydrogenase (LDH) levels in the remifentanil-treated group were significantly lower at 10 and 30 min post-reperfusion (15.3±7.1 and 10.2±6.8 U/l, respectively), as compared with the control group (29.7±8.3 and 20.6±6.8 U/l, respectively; P<0.05). The results of the present study suggested that the application of remifentanil following ischemia protected heart function via the opioid receptors by reducing myocardial enzyme release, and attenuating ischemia-induced changes to the myocardial cell and mitochondrial structure. © 2017, Spandidos Publications. All rights reserved.
Wang Y.,Shenxiang 705 Hospital |
Han H.-G.,The General Hospital of Shenyang Military Region
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: The visual recovery and complications of the cataract patients are various after different intraocular lens materials implantation, so the selection of appropriate intraocular lens materials is the key of the research. Objective: To investigate the properties and biocompatibility of intraocular lens materials. Methods: The materials were classified according to the hardness and location of the intraocular lens after implantation in order to analyze the pros and cons of the intraocular lens thus guiding the patients to select the appropriate intraocular lens for implantation. The properties and biocompatibility of the commonly used intraocular lens materials were evaluated. RESUTLS AND CONCLUSION: Polymethylmethacrylate intraocular lens is the first choice of rigid material, but it cannot tolerate autoclaving. Acrylic intraocular lens is the soft and foldable intraocular lens that has the similar optical and biological properties with polymethylmethacrylate intraocular lens, and it can improve the visual acuity after implantation with less complications. Posterior capsular opacification is the most important indicator for biocompatibility evaluation which is related with the material and design of intraocular lens. The stability of intraocular lens in the capsular bag is also the indicator to evaluate the biocompatibility. The material properties, biocompatibility, and visual function should be taking into consideration in the design and selection of intraocular lens materials. Guarantee the efficacy after intraocular lens implantation and reduce the incidence of complications, so that to get vision rehabilitation for the cataract patients.
Cao N.,The General Hospital of Shenyang Military Region
Chinese Journal of Tissue Engineering Research | Year: 2012
BACKGROUND: Hemodialysis is the main method for the treatment of acute and chronic renal failure; hemodialysis machine can purify the blood through diffusion, convection and filtration procedures by a semi-permeable membrane, and it can excrete the metabolic wastes and excess electrolyte in blood. Selection of the dialysis membrane is closely related with the effectiveness of hemodialysis. OBJECTIVE: To perform a multilevel analysis on the articles addressing hemodialysis membrane materials using the search and depth analysis capabilities of CNKI database. METHODS: The CNKI database was used to search articles related to hemodialysis membrane materials from January 2002 to December 2011 with the key words of "hemodialysis, dialysis membrane, biomaterials". The publishing time, number, subject category, source journals, fund assistance, author distribution, research institution, key words, cited frequency and download frequency of the articles were analyzed. We expressed the articles through text and charts. RESULTS AND CONCLUSION: A total of 95 articles were included for the analysis. Judging from the number of the articles, there was 20 articles published in 2002; the number of the articles published in Chinese Journal of Blood Purification was up to 19. In view of the key words, hemodialysis membrane was mainly used for the treatment of uremia, and the analysis of the hemodialysis membrane was mainly focused on the biocompatibility of hemodialysis membrane materials, including the effect of dialysis membrane on the oxidative stress and phosphate removal of the patients with maintenance hemodialysis. The articles regarding hemodialysis membrane in CNKI database were analyzed by bibliometric method which could provide the research ideas and valuable data for the workers engaged in the basic research and clinical implementation of hemodialysis membrane materials.
Overexpression of integrin-linked kinase correlates with malignant phenotype in non-small cell lung cancer and promotes lung cancer cell invasion and migration via regulating epithelial-mesenchymal transition (EMT)-related genes
Chen D.,Shenyang University |
Zhang Y.,The General Hospital of Shenyang Military Region |
Zhang X.,Shenyang University |
Li J.,Shenyang University |
And 6 more authors.
Acta Histochemica | Year: 2013
Integrin-linked kinase (ILK), a member of the serine/threonine kinases, has been implicated in oncogenesis and progression of human cancers. The aim of this study was to characterize the role of ILK in lung cancer aggressiveness and the underlying molecular mechanisms. ILK protein expression was assessed by immunohistochemistry in a cohort of non-small cell lung cancer (NSCLC) patients, and a series of in vitro assays was conducted to elucidate the function of ILK in lung cancer. Overexpression of ILK protein was detected in 30.6% (33/108) of primary NSCLC tissues and correlated with the TNM stage (P= 0.001) and lymph node metastasis (P= 0.033). Ectopic overexpression of ILK in lung cancer cells promoted cell migration and invasion in vitro, and led to the acquisition of epithelial-mesenchymal transition (EMT) phenotype, as evidenced by the spindle-like morphology, down-regulation of E-cadherin, and up-regulation of vimentin, fibronectin, Snail and Slug. In addition, the down-regulation of E-cadherin induced by ILK was significantly reversed by nuclear factor-κB (NF-κB) inhibitor BAY 11-7028 and small interfering RNA (siRNA) targeting NF-κB p65, suggesting a role of the NF-κB signaling pathway in ILK-induced EMT. Overall, our results suggest that ILK promotes lung cancer cell migration and invasion through the induction of EMT process. © 2012 Elsevier GmbH.
Zong C.,Shenyang University |
Wang J.,The General Hospital of Shenyang Military Region |
Shi T.-M.,Shenyang University
Tumor Biology | Year: 2014
MicroRNAs (miRNAs) have recently been identified as a novel class of gene regulators, playing an important role in various malignancies. In the present study, we investigated the role of miRNA-130b in the development of drug resistance in ovarian cancer cells. The human ovarian carcinoma cell line A2780 and paclitaxel-resistant A2780/Taxol cells were exposed to the chemotherapeutic agent cisplatin or paclitaxel in the presence or absence of transfected miR-130b. Cell viability assays were then performed using the Cell Counting Kit-8 (CCK-8) assay. Reverse transcription polymerase chain reaction and Western blotting were used to assess the messenger RNA (mRNA) and protein expression levels of glutathione S-transferase (GST)-π, multidrug resistance (MDR)1, or P-glycoprotein (P-gp). Following transfection, we found higher expression levels of miR-130b in A2780/Taxol cells than in A2780 cells (p < 0.05). Both A2780 and A2780/Taxol cells showed decreased sensitivity to paclitaxel and cisplatin compared with mock-transfected and negative control cancer cells (p < 0.05). The mRNA expression levels of MDR1 and GST-π (p < 0.05) and the protein expression levels of P-gp and GST-π were downregulated following miR-130b transfection in comparison to mock-transfected and negative control cancer cells. Our findings suggest that miRNA-130b may be involved in the development of drug resistance in ovarian cancer. © 2014, International Society of Oncology and BioMarkers (ISOBM).
Tang C.,The General Hospital of Shenyang Military Region |
Xue H.,The General Hospital of Shenyang Military Region |
Bai C.,Liaoning Medical University |
Fu R.,Liaoning Medical University |
Wu A.,Liaoning Medical University
Phytomedicine | Year: 2010
Disruption of blood-brain barrier (BBB) and edema formation play a key role in the development of neurological dysfunction after cerebral ischemia. In this study, the effects of Tanshinone IIA (Tan IIA), one of the active ingredients of Salvia miltiorrhiza root, on the BBB and brain edema after transient middle cerebral artery occlusion in rats were examined. Our study demonstrated that Tan IIA reduced brain infarct area, water content in the ischemic hemisphere. Furthermore, Tan IIA significantly decreased BBB permeability to Evans blue, suppressed the expression of intercellular adhesion molecule-1 (ICAM-1), matrix metalloproteinase-9 (MMP-9), inhibited the degradation of tight junction proteins zonula occludens-1 (ZO-1) and Occludin. These results demonstrated that Tan IIA was effective for attenuating the extent of brain edema formation in response to ischemia injury in rats, partly by Tan IIA's protective effect on the BBB. Our results may have implications in the treatment of brain edema in cerebral ischemia. © 2010 Elsevier GmbH.
Gao W.Y.,the General Hospital of Shenyang Military Region
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010
To verify the efficacy of Jianpi Tiaogan Wenshen Recipe (JTWR) in treating diarrhea-predominant irritable bowel syndrome (IBS-D) and to analyze its therapeutic mechanism through observing the effect of JTWR on clinical symptoms and rectal sensibility in patients. With a prospective, randomized controlled trial adopted, 80 patients with IBS-D were assigned randomly equally and to two groups. The treatment group was treated with JTWR, and the control group was treated with pinaverium bromide tablet (PVB), all for four weeks. Patients' symptoms, such as abdominal discomfort, pain, and distension; frequency of defecation; appearance of stool; and occurrence of tenesmus were recorded before and after treatment by scoring, and the rectal sensitivity was detected as well. Patients with therapeutic effect of cured and markly effective were followed up four weeks after withdrawal of medication. Three cases in the treatment group and four cases in the control group were dropped. Except the appearing of mucus stool, no statistically significant difference was shown between the two group in all other symptoms, either at before or after treatment; but the end point scores of individual symptoms between pre- and post-treatment were different statistically in both groups (P<0.05). Per-protocol population set (PPS) analysis on comprehensive effect showed that the total effective rate and the cure rate in the treatment group was 81.1% (30/37) and 24.3% (9/37), and those in the control group, 80.6% (29/36) and 19.4% (7/36) respectively; while the full analysis set (FAS) showed a result of 80.0% (32/40) and 22.5% (9/40) vs 77.5% (31/40) and 17.5% (7/40) respectively, all with insignificant difference between groups (P>0.05). Follow-up study showed that relapse or aggravation of disease occurred in four cases in the treatment group and 12 in the control group respectively, showing significant difference between groups (P<0.01). Rectal sensitivity examination showed that the rectal thresholds of sensation, defecation, and maximum tolerable volume were improved in both groups after treatment (P<0.05), but showed no significant difference between groups (P>0.05). JTWR is effective in treating IBS-D, with the effect better than PVB in improving mucus stool, also in the remote effect. Its therapeutic mechanism is possibly by way of adjusting the sensitivity of rectum.
Fan L.-H.,The General Hospital of Shenyang Military Region |
He L.,The General Hospital of Shenyang Military Region |
Cao Z.-Q.,The General Hospital of Shenyang Military Region |
Xiang J.,The General Hospital of Shenyang Military Region |
Liu L.,The General Hospital of Shenyang Military Region
International Braz J Urol | Year: 2012
Objective: To study the effect of ischemia preconditioning (IP) on renal ischemia/rep er-fusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats. Materials and Methods: The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., repeated four times. The Wistar rat kidneys used for warm ischemia preconditioning were subjected to 60 min of renal warm ischemia followed by reperfusion. The rat kidneys with ischemia/ reperfusion were compared with the ischemia preconditioning group to observe rat renal function and changes in the expression of renal adhesion molecules ICAM-1, P--Selectin, and E-Selectin. Results: The expression of rat renal adhesion molecules (ICAM-1, P-Selectin, and E-Se-lectin) with ischemia preconditioning was significantly lower than that of the ischemia/ reperfusion group. Serum creatinine was significantly lower than that in the ischemia/ reperfusion group after 48 hours. Conclusions: Ischemia preconditioning has a protective effect on renal function. Reduced expression of renal adhesion molecules is likely a mechanism involved in the observed protection.
Chen Y.-Z.,Shenyang University |
Fan Z.-H.,Chinese Medicine Hospital in Linyi City |
Zhao Y.-X.,Shenyang University |
Bai L.,Shenyang University |
And 3 more authors.
Tumor Biology | Year: 2014
To investigate the association of LIG1 with the risk of lung cancer, all subjects of unrelated ethnic Han Chinese in Liaoning Province were involved in a hospital-based case–control study. The case group consisted of 370 histologically diagnosed lung cancer patients; 314 controls were selected from cancer-free patients during Dec. 2009 to Dec. 2011. LIG1 rs1050298SNP were analyzed by TaqMan real-time PCR method. All statistical analyses were performed with Statistical Product and Service Solution sv13.0 (SPSS). The genotype distribution frequency of LIG1 rs1050298 SNP displayed significant difference between the case and the control group. Individuals carrying the LIG1 rs1050298 T genotype had higher risks of lung cancer, especially those with squamous cell carcinoma. © 2014, International Society of Oncology and BioMarkers (ISOBM).
PubMed | The General Hospital of Shenyang Military Region
Type: | Journal: Stem cells international | Year: 2017
Articular cartilage injury and defect caused by trauma and chronic osteoarthritis vascularity are very common, while the repair of injured cartilage remains a great challenge due to its limited healing capacity. Stem cell-based tissue engineering provides a promising treatment option for injured articular cartilage because of the cells potential for multiple differentiations. However, its application has been largely limited by stem cell type, number, source, proliferation, and differentiation. We hypothesized that (1) adipose-derived stem cells are ideal seed cells for articular cartilage repair because of their accessibility and abundance and (2) the microenvironment of articular cartilage could induce adipose-derived stem cells (ADSCs) to differentiate into chondrocytes. In order to test our hypotheses, we isolated stem cells from rabbit adipose tissues and cocultured these ADSCs with rabbit articular cartilage chondrocytes. We found that when ADSCs were cocultured with chondrocytes, the proliferation of articular cartilage chondrocytes was promoted, the apoptosis of chondrocytes was inhibited, and the osteogenic and chondrogenic differentiation of ADSCs was enhanced. The study on the mechanism of this coculture system indicated that the role of this coculture system is similar to the function of TGF-