Zhao W.,General Hospital of the Peoples Liberation Army |
Luo J.,Solomonbrothers Medical Institute |
Jiao S.,General Hospital of the Peoples Liberation Army
Scientific Reports | Year: 2014
Long non-coding RNAs (lncRNAs) are a kind of RNAs with regulation that participate fundamental cellular processes via diverse mechanisms. Despite the potential importance of lncRNAs in multiple kinds of cancer has been well studied, no comprehensive survey of cancer subtype associated lncRNAs. Here, we performed an array-based transcriptional survey of lncRNAs across 150 lung cancer samples comprising both adenocarcinoma and squamous cell carcinoma, and 306 breast cancer patients with clear clinical information. In lung cancer, 72 lncRNAs are identified to be associated with tumor subtypes and their functions as well as the associated proteins are predicted by constructing coding-non-coding co-expression network. The results suggest that they are mostly related with epidermis development, cell adhesion and response to stimulus. The validation results show the high concordance and confirmed the robust of the identification results. In breast cancer, we found 3 lncRNA genes are associated with estrogen receptor a (ER) positive and ER negative subtypes and tumor histologic grade. Survival (Kaplan-Meier) analysis results suggest that the expression pattern of the 3 lncRNAs is significantly correlated with clinical outcomes. The current study provides the first large-scale survey of lncRNAs within cancer subtypes, and may offer new targets for their diagnosis, therapy and prognosis.
Shen L.,PLA Fourth Military Medical University |
Sun X.,PLA Fourth Military Medical University |
Fu Z.,General Hospital of the Peoples Liberation Army |
Yang G.,PLA Fourth Military Medical University |
And 2 more authors.
Clinical Cancer Research | Year: 2012
It is well established that the altered metabolism exhibited by cancer cells, including high rates of glycolysis, lactate production, and biosynthesis of lipids, nucleotides, and other macromolecules, and which may occur either as a consequence or as a cause of tumorigenesis, plays an essential role in cancer progression. Recently, the tumor suppressor p53 was found to play a central role in this process. Here, we review the role of p53 in modulating tumor metabolism. Specifically, we focus on the functions of p53 in regulating aerobic glycolysis, oxidative phosphorylation, the pentose phosphate pathway, fatty acid synthesis and oxidation, and glutamine metabolism, and we discuss the therapeutic strategy whereby p53 helps to prevent malignant progression. © 2012 AACR.
Huang M.,PLA Fourth Military Medical University |
Wang H.-Q.,PLA Fourth Military Medical University |
Zhang Q.,General Hospital of the Peoples Liberation Army |
Yan X.-D.,PLA Fourth Military Medical University |
And 2 more authors.
Journal of Orthopaedic Research | Year: 2012
Intervertebral disc degeneration (IDD) pertains to the loss of extracellular matrix (ECM), particularly the early loss of aggrecan, the turnover of which is regulated by ADAMTSs. Amongst the etiological factors of IDD, mechanical stress plays an important role in the physiological and pathological processes of nucleus pulposus (NP) cells. However, the role of ADAMTSs and their inhibitor in human NP cells under mechanical stress has not been elucidated to date. The purpose of this study was to investigate the role of ADAMTSs and TIMP-3 in NP cells under mechanical stress. Human NP cells isolated from non-degenerative and degenerative discs were subjected to dynamic compressive load. The expression of ADAMTSs, aggrecan, and TIMP-3 was detected by quantitative real-time PCR and/or Western blot. Consequently, the gene expression of ADAMTS-1, 4, and 5 increased significantly in loaded NP cells compared with not-loaded cells from either non-degenerative or degenerative discs, whereas the gene expression of aggrecan decreased significantly. Moreover, Western blot indicated increased protein levels of ADAMTSs-1, 4, and 5. However, the expression of TIMP-3 altered insignificantly. Together, this study is the first addressing the underlying mechanisms of compressive load as a contributing factor to IDD in terms of ADAMTSs. Our results suggest that compressive load leads to the increase in ADAMTS-1, 4, and 5 that contributes to the decrease of aggrecan and IDD via TIMP-3 independent machinery. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc.
Wang M.,General Hospital of the Peoples Liberation Army |
Chen B.,PLA Fourth Military Medical University |
Wang J.,PLA Fourth Military Medical University |
Ma X.,PLA Fourth Military Medical University |
Wang Y.,General Hospital of the Peoples Liberation Army
Journal of Minimally Invasive Gynecology | Year: 2014
Study Objective: To evaluate outcomes relative to treatment using systemic methotrexate (MTX) alone or systemic MTX combined with ultrasound (US)-guided local injection of potassium chloride (KCl) or MTX in women with live tubal ectopic pregnancies. Design: Case-control study (Canadian Task Force classification II-2). Setting: Departments of Obstetrics and Gynecology in 2 hospitals in China. Patients: Eighty-two women with live tubal ectopic pregnancies. Intervention: Participants in the study received treatment using either systemic MTX (n=37; systemic treatment group) or systemic MTX and US-guided local injection of either MTX or KCl (n=45; combined treatment group). Measurements and Main Results: Patient clinical features and outcomes were compared. There were no significant differences between the patient groups insofar as baseline gestational age, β-human chorionic gonadotropin concentration, or size of conceptus. The success rate in patients who received combined therapy (93.3%) was much higher than in those who received only systemic treatment (73.0%) (p<.05). In the combined treatment group, the success rate was similar between women who received locally injected KCl (95.2%) and those who received locally injected MTX (91.7%). Conclusion: The significantly higher success rate in patients who received combined US-guided local injection and systemic MTX suggests that this is an efficient nonsurgical option in women with tubal pregnancy, high serum β-human chorionic gonadotropin concentration, and fetal cardiac activity. © 2014 AAGL.
Ke Q.,Zhejiang University |
Luo B.,Zhejiang University |
Qi M.,Zhejiang University |
Du Y.,Zhejiang University |
Wu W.,General Hospital of the Peoples Liberation Army
Muscle and Nerve | Year: 2013
Introduction: Hypokalemic periodic paralysis (HypoPP) is an autosomal dominant skeletal muscle ion channelopathy. Sex hormones are natural ion channel regulators. Different sex hormones have different effects on ion channels. A comparison of the penetrance and phenotype between males and females with HypoPP mutations should aid in proving that sex hormones play different roles in HypoPP and also provide the basis for the development of therapies against HypoPP. Methods: We identified all mutation carriers in 4 HypoPP families using PCR sequencing techniques. All patients underwent clinical investigation. Results: There were 8 men and 7 women mutation carriers in the 4 families. Male carriers had 100% penetrance, but female penetrance was only 28.57%. The highest attack frequency was 50-150 times/year for the men, whereas it was 30-50 times/year for the women. The attacks disappeared during pregnancy. Conclusions: The penetrance and attack frequency were lower in women than in men with HypoPP mutations. © 2012 Wiley Periodicals, Inc.
Dang H.-Z.,General Hospital of the Peoples Liberation Army |
Dang H.-Z.,401 Hospital of the Peoples Liberation Army |
Yu Y.,Chinese People's Liberation Army |
Jiao S.-C.,General Hospital of the Peoples Liberation Army
World Journal of Gastroenterology | Year: 2012
AIM: To study the risk factors for liver metastasis and the prognosis in patients with human epidermal growth factor receptor 2 (HER2) over-expressing gastric cancer (GC). METHODS: A total of 84 GC patients recruited from the General Hospital of the People's Liberation Army (PLA) between 2003 and 2010 were randomly enrolled in this study. HER2 expression was detected by immunohistochemistry in 84 GC patients with liver metastases. The study group consisted of 66 men and 18 women, with an average age of 54 years (range: 19-74 years). Liver metastasis was diagnosed by magnetic resonance imaging or computed tomography. Patients were followed-up and predictive factors of liver metastasis were evaluated. RESULTS: The median follow-up period was 47 mo (range: 6-85 mo). The characteristics of 35 (25.7%) patients with HER2 over-expression of liver metastatic GC are presented. HER2 over-expression was detected in 23 out of 49 (46.9%) patients with intestinal GC, and 9 out of 35 (25.7%) patients with diffuse GC. 29 out of 59 (49.2%) patients aged < 60 years were HER2-positive, while 8 out of 25 (32%) patients aged = 60 were HER2-positive; a significant difference (P < 0.05). Univariate analysis (log-rank test) showed that HER2 over-expression, sex, Lauren classification, differentiation and disease-free interval were correlated with poor survival (P < 0.05). Survival analysis with a survival curve showed that HER2 over-expression was significantly relevant, with a reduced survival time in GC patients with liver metastases (P < 0.01). 2-year survival was not associated with the patient's age. A disease-free survival longer than 12 mo has a significant association with extended overall survival (OS) in GC patients with liver metastases. The median survival time after the diagnosis of liver metastases was 18 mo [95% confidence interval (CI): 9.07-26.94] among HER2 positive GC patients with liver metastases. In comparison, for 49 (69.4%) out of 84 HER2 negative patients with liver metastatic GC, the median survival time was 47 mo (95% CI: 19.37-74.63). In patients with HER2 positive liver metastatic GC, the median OS was significantly shorter than in HER2 negative patients (median, 20.32 mo; 95% CI: 16.51-24.13 vs median, 50.14 mo; 95% CI: 37.83-62.45; P < 0.01). CONCLUSION: HER2 over-expressing GC patients with liver metastases have a poor prognosis. Overall survival was significantly lower in HER2 positive patients. HER2-overexpression is correlated with a lower survival rate. © 2012 Baishideng. All rights reserved.
Chen C.,Second Hospital of Tangshan |
Tang P.,General Hospital of the Peoples Liberation Army |
Zhang X.,Second Hospital of Tangshan
Plastic and Reconstructive Surgery | Year: 2012
Background: This article reports sensory reconstruction of a finger pulp defect using a dorsal homodigital island flap including double dorsal branches of the proper digital nerves. Methods: From February of 2008 to December of 2009, the dorsal homodigital island flap was used in 15 fingers in 15 patients. The average patient age was 32 years. The injured digits included six index, six long, and three ring fingers. The mean size of the finger pulp defects was 2.4 × 2.0 cm, the mean flap size was 2.5 × 2.1 cm, and the mean pedicle length was 1.2 cm. Neurorrhaphy was performed between the dorsal branches of the proper digital nerves and the proper digital nerves at the recipient site. Flap sensation was assessed using static two-point discrimination and Semmes-Weinstein monofilament testing. For comparison, 28 patients treated using a cross-finger flap including a single nerve branch from February of 2005 to October of 2007 were included. Results: In the study group, all flaps survived completely. At a mean follow-up of 19 months, the mean static two-point discrimination and Semmes-Weinstein monofilament scores on the pulp were 5.8 mm and 3.94, respectively. In the comparison group, the scores were 8.4 mm and 4.11, respectively. There was a significant difference between the two groups in static two-point discrimination and no significant difference in Semmes-Weinstein monofilament scores. Conclusions: The dorsal homodigital island flap is an alternative for finger pulp reconstruction. The authors suggest performing double neurorrhaphies to improve flap sensation. Copyright © 2012 by the American Society of Plastic Surgeons.
Wang T.,General Hospital of the Peoples Liberation Army
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2010
To assess the value of positron emission tomography (PET) with (11)C-choline (CH), (11)C-methionine (MET), (18)F-fluorothymidine (FLT), and (11)C-acetate (AC) in diagnosis of pulmonary abnormalities and the features of pulmonary abnormalities in PET. From June 2002 to June 2007, 100 patients with pulmonary nodules or masses confirmed by CT scans received PET with special tracers. Fifty-eight patients received CH-PET, 16 patients received MET-PET, 22 patients received FLT-PET, 4 patients received AC-PET. PET data was analyzed by visual method and semiquantitative method with standard uptake value (SUV). Diagnoses were compared with pathology and follow-up survey. For identification of pulmonary neoplasms with CH-PET, the sensitivity, specificity and accuracy were 84.2% (32/38), 57.9% (11/19) and 75.4% (43/57). In cancer cases, SUV had no correlation with tumor size or age. For identification of pulmonary neoplasms with MET-PET, the sensitivity, specificity and accuracy were 6/7, 6/9 and 75.0% (12/16). In cancer cases, SUV had not correlation with tumor size or age. For identification of pulmonary neoplasms with FLT-PET, the sensitivity, specificity and accuracy were 85.7% (12/14), 2/8 and 63.6% (14/22). In cancer cases, SUV had not correlation with tumor size or age. In AC-PET, only 1 case of pulmonary metastasis of kidney clear cell carcinoma showed acetate avid. Two squamous cell carcinoma and 1 adenocarcinoma didn't appear abnormal in AC-PET. CH, MET, FLT, AC are valuable in diagnosing but also lead to false positive and false negative.
Zhu J.,General Hospital of the Peoples Liberation Army
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2010
To analyze the influence of benign prostatic hyperplasia (BPH) drugs on incidence and pathology grading of prostate cancer in China. Retrospectively investigated the history of drug treatment in 1029 cases of BPH in patients from February 1998 to December 2004. According to the history of drug use, the patients were divided into 4 groups: finasteride group, alpha-receptor inhibitor group, finasteride and alpha-receptor inhibitor combination group and control group (untreated group). We gathered pathology sections of patients in all groups, and gave Gleason Score to each. The difference of incidence and pathology grading of prostate cancer were analyzed by Stata 7.0. The incidence of prostate cancer in the population of our study was 13.5%; The incidence in finasteride group, alpha-receptor inhibitor group, combination group and control group was 9.8%, 16.0%, 10.3% and 18.6%, respectively. There was significant difference between the two groups with the use of finasteride and the two groups without it (P < 0.05). In our study, the ratio of middle or high level pathology grading (Gleason ≥ 7) in prostate cancer patients was 58.3%, the ratio of middle or high level pathology grading prostate cancer patients in the four groups was 71.4%, 59.6%, 67.7% and 40.0%, respectively. In the comparison of composition ratio of middle or high level prostate cancer, there was significant difference between the two groups with the use of finasteride and the two groups without it (P < 0.05). Finasteride can lower the risk of prostate cancer, but increase the pathology grade of the prostate cancer which has occurred in the same time. The alpha-receptor inhibitor does not have the same effect.
Guo A.-T.,General Hospital of the Peoples Liberation Army |
Li Y.-M.,General Hospital of the Peoples Liberation Army |
Wei L.-X.,General Hospital of the Peoples Liberation Army
World Journal of Gastroenterology | Year: 2012
AIM: To assess the clinicopathologic features and its relationship with prognosis of pseudomyxoma peritonei (PMP) in Chinese patients. METHODS: The clinicopathologic features and follow-up data of 92 patients with PMP were reviewed and retrospectively analyzed. The cases were categorized into three groups: disseminated peritoneal adenomucinosis (DPAM), peritoneal mucinous carcinomatosis (PMCA), and peritoneal mucinous carcinomatosis with intermediate or discordant features (PMCA-I/D). The log-rank test was used to analyze survival for each group and various clinicopathological parameters. Multivariate Cox proportional-hazard models were constructed to determine the important factors associated with survival. RESULTS: The median age at diagnosis was 51.9 years (range: 22-76 years). The median follow up was 124 mo. The 3-, 5-and 10-year survival rates were 74.0%, 67.4% and 49.1%, respectively. There were 49 (53.2%) patients with DPAM, 26 (28.3%) with PMCA-I and 17 (18.5%) with PMCA. Patients with DPAM, PMCA-I/D and PMCA exhibited statistically significant difference in survival (P = 0.001). The 3 year survival for DPAM, PMCAI/D and PMCA was 97.0%, 80.0% and 67.0%, respectively; the 5 year survival was 80.0%, 67.0% and 50.0%, respectively; and the 10 year survival was 65.0%, 28.0% and 14.0%, respectively. Survival rate was significantly lowest in patients < 40 age years of age (P = 0.011). Appendiceal tumor and extra-ovarian parenchymal organ involvement were significantly related to overall survival. Patients with appendiceal mucinous adenocarcinoma (MACA) showed the significantly poorer prognosis (P = 0.011). Multivariate analysis showed that pathological classification, age, appendiceal tumor were significant related to overall survival. CONCLUSION: The clinical process "PMP" should be pathologically classified into DPAM, PMCA and PMCA-I/ D. Pathological classification, age, appendiceal MACA are survival independent predictors in Chinese patients with PMP. © 2012 Baishideng. All rights reserved.