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Zhang L.,The General Hospital of Jinan Military Command
International immunopharmacology | Year: 2013

11β-Hydroxysteroid dehydrogenase 1 (11β-HSD1) plays an important role in inflammation. However, the role of 11β-HSD1 in rheumatoid arthritis (RA) remains unknown. The purpose of this study was to evaluate the therapeutic effects of a selective 11β-HSD1 inhibitor BVT-2733 in collagen-induced arthritis (CIA) and its underlying mechanisms. CIA mice were treated with BVT-2733 (100 mg/kg, orally) or vehicle twice daily for 2 weeks. Arthritis score and joint histology were investigated. The levels of pro-inflammatory cytokines as well as anti-type II collagen antibody (anti-CII) were detected by ELISA. Western blot analysis was used to assess the activation of NF-κB and NLRP1 inflammasome in joint tissues and in human RA synovial cells. BVT-2733 treatment attenuated the arthritis severity and anti-CII level in CIA mice. BVT-2733 also decreased the levels of serum TNF-α, IL-1β, IL-6 and IL-17. BVT-2733 treatment also significantly reduced synovial inflammation and joint destruction. NF-κB activation and NLRP1 inflammasome assembly were also inhibited in arthritic joints and human RA synovial cells. In conclusion, BVT-2733 exhibits an anti-inflammatory effect on CIA. This protective effect is, at least partly, mediated by inhibition of the NF-κB and NLRP1 inflammasome signaling pathways. 11β-HSD1 inhibition may represent a potential therapeutic target for RA patients. © 2013.

Wang Z.,456 Hospital of PLA | Li H.,456 Hospital of PLA | Cao H.,456 Hospital of PLA | Zheng J.,The General Hospital of Jinan Military Command
International Journal of Clinical and Experimental Pathology | Year: 2014

Objective: We studied the clinicopathological features of type AB thymoma with liver metastases and explore the histological types, diagnosis and differential diagnosis, treatment and prognosis for this disease. Methods: Liver metastasis specimens were derived from one case of type AB thymoma 5 years after operation and were examined histologically using light microscopy and immunohistochemistry. A comprehensive analysis was performed on the patient's clinical manifestations, histopathology, immunohistochemistry features, differential diagnosis, treatment and prognosis in combination with a review of the relevant literature. Results: Metastases were detected 5 years after initial operation on a 49-year-old, female patient with type AB thymoma. The pathological diagnosis for the liver metastases was type AB thymoma. Immunohistochemistry staining showed CKpan (+), CD3 (+), CD2 (+), TdT (+), CD5 (+), P53 (+), Hep-1 (-), and Ki67-positive cells (25%). The expression level of galectin-3 was higher than in the mediastinal thymoma tissue identified 5 years prior. Conclusion: Liver metastases of type AB thymoma are extremely rare. This case indicated that type AB thymoma with low malignant potential might recur and develop distant metastases. Overexpression of p53, galectin-3 and Ki67 in type AB thymoma might coordinately manipulate the process of development, progression and malignant transformation of type AB thymomas.

Li X.-L.,Hainan Branch of PLA General Hospital | Liu Z.-Y.,Chinese PLA General Hospital | Zhou N.,Chinese PLA General Hospital | Zhu T.,The General Hospital of Jinan Military Command | And 2 more authors.
International Journal of Surgery | Year: 2016

Objective: To evaluate the long-time clinical outcomes of robotic sacral hysteropexy for pelvic organ prolapse (POP). Methods: Five women who underwent robotic sacral hysteropexy for the treatment for POP. Blood loss, operative time, length of stay, blood transfusion, pulmonary embolus, gastrointestinal or genitourinary tract injury, ileus, bowel obstruction, post-operative fever, and urinary retention were recorded for all patients. Results: All the operative procedures were successfully performed using the robotic approach. In one case with perineal laceration, perineal repair was simultaneously performed, and in one patient with combined leiomyoma, myomectomy was performed first. The other three cases underwent no additional procedures during the surgery. Neither intra-nor post-operative complications occurred in all 5 cases. After follow-up one year, all patients declared their satisfaction with the achieved anatomical and functional results. Conclusions: The robotic sacral hysteropexy is a minimally invasive technique for POP repair. We found low complication rates and high patient satisfaction with a minimum of 1 year followup. Larger series with longer follow-up data are needed to justify its widespread use. © 2016 IJS Publishing Group Limited.

Yin D.-P.,The General Hospital of Jinan Military Command | Liu L.,Shanghai JiaoTong University
International Eye Science | Year: 2013

The therapy and functional recovery of optic nerve injury is a historical tough problem. Because after the optic nerves, as a part of central nervous system, were injured, the optic nerves were short of the microenvironment which nerves need to repair and regeneration. For that, it is very important that the effective nerves' protection, the prevention of neurons' death and to promote nerves' renovation. A great quantity of investigation has demonstrated that the visual function's recovery is closely related to the injury degree of retinal ganglial cells(RGCs), the axoplasm conveying materiae synthesis functional status, the self reparation capability and the self regeneration capability. In the recent decade, with the comprehending to the injury mechanism of nerves, a kind of researches in nerve preserved showed fairly advancements, which have revealed an excellent perspective in the part of healing the optic nerve injury. We read the recent pertinent literatures in the country of exterior and interior and composed a review concerning with the empirical study and the clinical therapy correlated to the RGCs' regeneration, the optical nerves' protection after the optic nerves injured. Copyright 2013 by the IJO Press.

Yin D.-P.,The General Hospital of Jinan Military Command
Chinese Journal of Ophthalmology | Year: 2013

Objective: This study is to investigate the effect of the combination of the olfactory ensheathing cells (OEC) transplantation and intravitreous injection of ciliary neurotrophic factor (CNTF) on the retinal ganglial cells (RGC) apoptosis and axonals' reparation and regeneration. Methods: In this study, the supraorbital margin exposure of the optic nerves was used to establish adult SD rats' optic nerve inhausted injury model as control group. Then the purified OECs were injected into the optic nerve sheaths, and CNTF was injected into the vitreous body simultaneously. The rats were divided into control group, CNTF group, OECs group, and OEC + CNTF combined group. At 4 weeks postoperatively, a cholera toxin B subunit (CTB) anterograde tracing technique and fluorescence (FG) biotinylated dextran amine (BDA) retrograde tracing technique were adopted to evaluate the survival of RGC and the regeneration of optic nerve axons. The number of survival neurons of the same vision field and the density of neurons were evaluated by analysis of variance. Results: At the one and three quadrant distance between optic disc 2 mm spot, the number of the RGC in the control group was significantly (F=633.38, P < 0.01) decreased compared with the normal group (46.00 ± 1.42, 34.80 ± 1.34, 25.00 ± 1.61, 15.40 ± 2.30). The survived RGC in OEC group was significantly more than that in CNTF group, while the combined treatment with CNTF and OEC had strongest repair effect. The neuron axon density showed a statistically significant difference in the average optical density value at distance between foramen opticum 2 mm spot (OEC + CNTF: 3.18 ± 0.26, OEC: 2.96 ± 0.28, CNTF: 2.83 ± 0.37, and control: 2.75 ± 0.12, respectively, F=17.66, P < 0.01). Conclusions: Optic nerves damage can be repaired and regenerated by the combined treatment with OEC and CNTF suggesting that CNTF and OEC have synergistic effect on the treatment of optic nerve injury and repair. Transplantation of OECs may genetically modify the secretion of human CNTF and promote optic nerve injury repair. (Chin J Ophthalmol, 2013, 49: 1020-1028). Copyright © 2013 by the Chinese Medical Association.

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