Jia N.,The Fourth Peoples Hospital of Shenzhen |
Xu J.,Fujian Medical University |
Zhao H.,Qiqihar Medical University |
Wang Y.,Qiqihar Medical University |
And 3 more authors.
Clinica Chimica Acta | Year: 2015
The aim of this study was to examine the individual and interactive associations of five non-synonymous variants of four DNA repair relevant genes (XRCC1, XRCC3, hOGG1, NQO1) with hypertension in a large northeastern Han Chinese population. This was a hospital-based study involving 1009 hypertensive patients and 756 normotensive controls. All five variants satisfied the Hardy-Weinberg equilibrium. With a Bonferroni corrected alpha of 0.05/5, significance was only attained in the genotype (P. =. 0.007) and allele (P. =. 0.006) distributions of rs25487 in XRCC1 gene between patients and controls, with its mutant allele conferring 29% (95% CI: 1.09-1.53; P. =. 0.003), 31% (95% CI: 1.05-1.62; P. =. 0.015) and 66% (95%CI: 1.10-2.52; P. =. 0.016) increased risks of hypertension under the additive, dominant and recessive models, respectively after adjusting for confounders. The frequency of allele combination C-A-C-G-C (alleles in order of rs1799782, rs25487, rs861539, rs1052133 and rs1800566) was significantly higher in patients than in controls (P. =. 0.003), while that of C-G-C-C-C was significantly lower (P. =. 0.001). Interaction analysis failed to identify any suggestive evidence of synergism across five examined variants. Our findings provide evidence for a contributory role of XRCC1 gene rs25487 variant in the development of hypertension, and this variant possibly acted in a recessive pattern. © 2014 Elsevier B.V.
Huang J.,University of Hong Kong |
Song G.,The Third Peoples Hospital Of Shenzhen |
Yin Z.,The Fourth Peoples Hospital Of Shenzhen |
Fu Z.,The Fourth Peoples Hospital Of Shenzhen |
Ye Z.,The Fourth Peoples Hospital Of Shenzhen
Archives of Rheumatology | Year: 2016
Objectives: This study aims to determine the levels of bone turnover markers in canonical wingless pathway in patients with ankylosing spondylitis (AS) and the correlation with disease activity indexes. Patients and methods: We recruited a total of 43 AS patients (34 males, 8 females; mean age 36.8±9.3 years; range 22 to 62 years) and age- and sex-matched 42 healthy controls (32 males, 10 females; mean age 36.1±9.7; range 24 to 59 years). Serum levels of components of canonical wingless pathway including Dickkopf-1, glycogen synthase kinase-3β, β-catenin, alkaline phosphatase, and osteocalcin were detected using enzymelinked immunosorbent assay method. All patients were assessed in terms of erythrocyte sedimentation rate, C-reactive protein, Bath Ankylosing Spondylitis Disease Activity Index, Bath Ankylosing Spondylitis Functional Index, Ankylosing Spondylitis Disease Activity Score, and the modified Stoke’s Ankylosing Spondylitis Spine Score. Pearson’s correlation test was used to analyze the correlation between serum bone turnover markers and clinical assessment indexes. Results: No significant difference was observed between AS patients and healthy controls for the levels of glycogen synthase kinase-3β, β-catenin, alkaline phosphatase, and osteocalcin, respectively (p>0.05). The level of Dickkopf-1 was significantly higher in AS patients (1914.5±407.8 pg/mL) than in healthy controls (1729.1±352.9 pg/mL) (p<0.05). There was no correlation between high Dickkopf-1 level and any of the clinical parameters contributing to inflammation or bone formation. However, the correlation between osteocalcin and disease duration was significant in AS patients (r=0.323, p=0.034). Conclusion: Alteration of bone turnover markers in canonical wingless pathway was observed in AS. This might partially explain the complicated mechanism of bone formation in the disease. © 2016 Turkish League Against Rheumatism. All rights reserved.
PubMed | The Third Peoples Hospital of Shenzhen, University of Hong Kong and The Fourth Peoples Hospital of Shenzhen
Type: Journal Article | Journal: Clinical rheumatology | Year: 2016
This was an exploratory analysis comparing the safety and efficacy of immunoadsorption (IAS) combination therapy in severe systemic lupus erythematosus (SLE) receiving corticosteroid pulse and immunosuppressant treatment. Patients enrolled all had predominant organ involvement including proteinuria, thrombocytopenia, pericardial effusion, and cerebral involvement requiring corticosteroid pulse treatment. Fifty-two patients in study group received IAS plus corticosteroid and cyclophosphamide treatment. Fifty-two patients in non-IAS group received corticosteroid and cyclophosphamide treatment. Outcome measurement included C3, dsDNA, AnuA, and SLE disease activity index (SLEDAI) 2k score and in particular cases, proteinuria quantification and platelet count. Disease activity dropped significantly in both groups. Improvement of disease activity markers was more significant in study group than that in non-IAS group. The lower dosage of steroid in study group suggested the steroid-sparing effect of IAS. No severe adverse effect occurred during IAS. Our study suggested IAS as an additional therapy to steroid pulse and immunosuppressant in treating severe SLE.
Fu W.-M.,Chinese University of Hong Kong |
Zhang J.-F.,Chinese University of Hong Kong |
Wang H.,Chinese University of Hong Kong |
Xi Z.-C.,Shanghai University of Traditional Chinese Medicine |
And 9 more authors.
Journal of Proteomics | Year: 2012
Hepatocellular carcinoma (HCC) is a global public health problem which causes approximately 500,000 deaths annually. Considering that the limited therapeutic options for HCC, novel therapeutic targets and drugs are urgently needed. In this study, we discovered that 1,3,5-trihydroxy-13,13-dimethyl-2H-pyran [7,6-b] xanthone (TDP), isolated from the traditional Chinese medicinal herb, Garcinia oblongifolia, effectively inhibited cell growth and induced the caspase-dependent mitochondrial apoptosis in HCC. A two-dimensional gel electrophoresis and mass spectrometry-based comparative proteomics were performed to find the molecular targets of TDP in HCC cells. Eighteen proteins were identified as differently expressed, with Hsp27 protein being one of the most significantly down-regulated proteins induced by TDP. In addition, the following gain- and loss-of-function studies indicated that Hsp27 mediates mitochondrial apoptosis induced by TDP. Furthermore, a nude mice model also demonstrated the suppressive effect of TDP on HCC. Our study suggests that TDP plays apoptosis-inducing roles by strongly suppressing the Hsp27 expression that is specifically associated with the mitochondrial death of the caspase-dependent pathway. In conclusion, TDP may be a potential anti-cancer drug candidate, especially to cancers with an abnormally high expression of Hsp27. © 2012 Elsevier B.V.
Xu J.,Guangdong Medical College |
Xiang L.,The Fourth Peoples Hospital of Shenzhen |
Chen J.,The Fourth Peoples Hospital of Shenzhen |
He Q.,The Fourth Peoples Hospital of Shenzhen |
And 3 more authors.
Photodiagnosis and Photodynamic Therapy | Year: 2013
Background: We evaluate the effectiveness of combination treatment using photodynamic therapy after carbon dioxide laser in preventing the recurrence of condylomata acuminata for intraanal warts in HIV positive homosexual men. A retrospective survey of 41 patients referred to the STD clinic of the Fourth People's Hospital of Shenzhen for the treatment of intraanal warts among HIV positive homosexual men between Janurary 2009 and September 2011. Methods: The patients who accepted CO2 laser monotherapy for the same diagnosis were used as the comparison group. After the confirmation of the diagnosis of HIV infection and intraanal warts, the patients were treated with the combination treatment using PDT with 5-ALA thermal gel immediately after CO2 laser ablation of the warts. PDT was performed with irradiation of 100J/cm2 at an irradiance of 100-150mW/cm2 with a semiconductor laser, wavelength 635nm. PDT therapy was repeated twice with 2 weekly intervals. Follow up examinations including an anoscopy every 4 weeks after the latest PDT. Results: After 3 cycles of PDT treatments, 39 cases of anoscopy examination showed no new or recurrent lesions. At the end of the sixth months, recurrence occurred in 12(29%) cases. HPV 11 was present in 6 (50%) of these recurrences. HPV 6/11 or 16/18 remains positive in 19 cases (46.3%) and HPV negative in 22 cases. Conclusions: The combination treatment with CO2 laser and PDT is much more effective in reducing the treatment cycles and the time intervals of the whole treatment for intraanal warts in HIV infected people. © 2012 Elsevier B.V.
Wu Z.L.,The Fourth Peoples Hospital of Shenzhen
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2010
To see the HBV DNA detection instance in the HBsAg negative people and to study the serological method detection strategy for detecting hepatitis B virus large surface protein (HBLP) to filtrate the occult HBV infection. The HBsAg negative serum samples were divided into HBsAb negative and positive two species according to the hepatitis B virus markers (HBVM) in daily work excepting the special HBVM modes. Total 2000 stochastic serum samples with 1000 HBsAb negative results and 1000 HBsAb positive results were collected from the copy tubes to detect HBVM with national ELISA reagent kits and put them -20 degrees C frostily. Mixed samples (8 x 30 microl) were analyzed with fluorescence quantitative PCR (FQ-PCR) and filtrated the individual positive samples. The filtrated samples were doubly tested again with American MONOLISA HBsAg ULTRA reagents. No HBV DNA positive results were found out from the 1000 HBsAb positive samples and 19 cases HBV DNA positive results were found out from the 1000 HBsAb negative samples. On these 19 samples, the HBsAg results from the American MONOLISA HBsAg ULTRA reagents were all positive and the HBLP results were all positive, too. The 19 HBV DNA quantitative results were divided into 2 cases more than 500 copies/ml, 3 cases between 400-500 copies/ ml, 3 cases between 300-400 copies/ml, 7 cases between 200-300 copies/ml and 4 cases between 100-200 copies/ml. The leaked samples tested HBsAg with national reagents are mostly from the HBsAb negative people. HBLP results may be positive on these samples and detecting HBLP marker is propitious to filtrate the occult HBV infection. This study provided a kind of serological reference for actively searching for the detecting strategy in occult HBV infection field.
PubMed | East China University of Science and Technology and the Fourth Peoples Hospital of Shenzhen
Type: Journal Article | Journal: European journal of mass spectrometry (Chichester, England) | Year: 2016
Amyloid-degrading enzyme neprilysin (NEP) plays a pivotal role in eliminating A The oxidized modification of NEP by 4-hydroxy-2-nonenal (HNE) may reduce the clearance of A in cultured cells and Alzheimers disease (AD) brains. The aim of this research is to study whether HNE could modify the NEP protein and identify the specific sites of HNE-NEP modification using a linear trap quadrapole (LTQ) Velos Pro-Orbitrap Elite mass spectrometer. NEP activity was determined after SH-SY5Y cells had incubated with HNE (20 M) for 24 hours. To identify the sites of NEP modification, samples of both native and HNE-modified NEP digested by trypsin were analyzed using a LTQ Velos Pro-Orbitrap Elite mass spectrometer. The NEP peptide sequence information from the fragment ion masses was used to search for the sites of NEP adduction. HNE-treated cells showed a 60% loss of NEP activity. NEP was covalently adducted at Lys 93, Lys 472 by HNE via Michael addition. Compared to the control group, the sites of modified peptide in NEP showed a consistent 156 Da increased in m/z, which provides sequence information and might contribute to further studies on drug design and the therapeutics of AD.
Wang H.,The Fourth Peoples Hospital Of Shenzhen |
Lu X.-H.,The Fourth Peoples Hospital Of Shenzhen |
Zuo W.,The Fourth Peoples Hospital Of Shenzhen
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Allogeneic penetrating keratoplasty is the most effective method for treating corneal blindness. However, the incidence of rejections is high after keratoplasty, so it is urgent to develop an immunosuppressive drug with high efficacy and low toxicity. OBJECTIVE: To establish allogeneic penetrating keratoplasty models and monitor the expression of tumor necrosis factor-a in blank control group and after transforming growth factor-β1 eyedrop during acute rejection period of corneal grafts. METHODS: Allogeneic penetrating keratoplasty models were established and were randomly divided into blank control group, ciclosporin A group (1% ciclosporin A), and transforming growth factor-β1 group (1 µg/ml transforming growth factor-pi eyedrop). The medications from each group commenced at 1 day after surgery, one eyedrop once, three eyedrops per day. All the operated eyes were given 0.3% ofloxacin ophthalmic solutions and 0.5% tropicaide ophthalmic solution, three times per day, for 12 days. The corneal grafts were harvested for hematoxylin-eosin staining and immunehistochemical staining (SABC method), to detect tumor necrosis factor-a expression in corneal grafts. RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that, corneal grafts were significantly thickened, a large number of histoleucocytes and lymphocytes infiltrated in the blank control group; corneal grafts showed normal thickness and no inflammatory cells infiltrated in the transforming growth factor-p1 group. Immunohistochemical staining showed that, there were less cells positive for tumor necrosis factor-a in the transforming growth factor-β1 group compared with the blank control group (P < 0.05). Transforming growth factor-β1 eye drops can reduce the expression of tumor necrosis factor-a in the corneal grafts during acute rejection period, and reduce the inflammatory cells infiltration in the corneal grafts, which is probably the mechanism of transforming growth factor-p1 to prevent and treat corneal allograft rejection.