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Wang H.,The Fourth Peoples Hospital of Jinan
International Journal of Clinical and Experimental Medicine | Year: 2016

The study was to compare carbohydrate antigen (CA) 242 and CA19-9 in lesion tissue and serum, and explore the association with gastric precancerous lesions. Sixty-two patients were divided into gastric precancerous lesion group (32 patients with atrophic gastritis), and chronic superficial gastritis (CSG) group (30 patients with CSG). The gastric precancerous lesion group was further divided into moderate precancerous lesion (16 with atrophic gastritis and moderate gastric dysplasia) and severe precancerous lesion subgroups (16 with atrophic gastritis and severe gastric dysplasia). Serum and tissue samples were acquired from each patient. CA242 and CA19-9 levels in serum and tissue were measured by enzyme-linked immunoabsorbent assay and electro-chemiluminescence method, respectively. For the gastric precancerous lesion group, CA242 and CA19-9 in lesion tissue were significantly higher than those in serum (P<0.01). Besides, CA242 and CA19-9 in lesion tissue were significantly increased in the gastric precancerous lesion group compared with the CSG group (P<0.01). CA242 and CA19-9 in lesion tissue weresignificantly higher in severe precancerous lesion subgroup than those in moderate precancerous lesion subgroup (P<0.01). However, no significant difference was detected in serum CA242 and CA19-9 between the two groups (P>0.05). CA242 and CA19-9 in lesion tissue might aid indistinguishing gastric precancerous lesion from CSG, and is associated with gastric precancerous lesion severity. © 2016, E-Century Publishing Corporation. All rights reserved. Source


Bian H.,Shandong University | Bian W.,The Fourth Peoples Hospital of Jinan | Lin X.,Shandong University | Ma Z.,Shandong University | And 2 more authors.
Neurochemical Research | Year: 2016

To explore the effect of glycogen synthase kinase 3β (GSK-3β) silencing on Tau-5 phosphorylation in mice suffering Alzheimer disease (AD). GSK-3β was firstly silenced in human neuroblastoma SH-SY5Y cells using special lentivirus (LV) and the content of Tau (A-12), p-Tau (Ser396) and p-Tau (PHF-6) proteins. GSK-3β was also silenced in APP/PS1 mouse model of AD mice, which were divided into three groups (n = 10): AD, vehicle, and LV group. Ten C57 mice were used as control. The memory ability of mice was tested by square water maze, and the morphological changes of hippocampus and neuron death were analyzed by haematoxylin–eosin staining. Moreover, the levels of Tau and phosphorylated Tau (p-Tau) were detected by western blotting and immunohistochemistry, respectively. The lentivirus-mediated GSK-3β silencing system was successfully developed and silencing GSK-3β at the cellular level reduced Tau phosphorylation obviously. Moreover, GSK-3β silence significantly improved the memory ability of AD mice in LV group compared with AD group (P < 0.05) according to the latency periods and error numbers. As for the hippocampus morphology and neuron death, no significant change was observed between LV group and normal control. Immunohistochemical detection and western blotting revealed that the levels of Tau and p-Tau were significantly down-regulated after GSK-3β silence. Silencing GSK-3β may have a positive effect on inhibiting the pathologic progression of AD through down-regulating the level of p-Tau. © 2016 Springer Science+Business Media New York Source


Guo L.,Shandong University | Liu Y.,Shandong University | Ding Z.,The Fourth Peoples Hospital of Jinan | Sun W.,Shandong University | Yuan M.,Shandong University
Oncology Letters | Year: 2016

The present study aimed to investigate the potential mechanisms used during signal transduction by M3 muscarinic acetylcholine receptor (CHRM3) in prostate cancer. The microarray datasets of GSE3325, including 5 clinically localized primary prostate cancers and 4 benign prostate tissues, were downloaded from the Gene Expression Omnibus database. The differentially-expressed genes (DEGs) in primary prostate cancer tissues compared with benign controls were screened using the Limma package. Gene Ontology and pathway enrichment analyses were performed using the Database for Annotation Visualization and Integrated Discovery. Next, a protein-protein interaction (PPI) network was constructed. Additionally, microRNAs (miRNAs) associated with DEGs were predicted and miRNA-target DEG analysis was performed using a Web-based Gene Set Analysis Toolkit. Finally, the PPI network and the miRNA-target DEG network were integrated using Cytoscape. In total, 224 DEGs were screened in the prostate cancer tissues, including 113 upregulated and 111 downregulated genes. CHRM3 and epidermal growth factor (EGF) were enriched in the regulation of the actin cytoskeleton. EGF and v-myc avian myelocytomatosis viral oncogene homolog (Myc) were enriched in the mitogen-activated protein kinase (MAPK) signaling pathway. EGF with the highest degree of connectivity was the hub node in the PPI network, and miR-34b could interact with Myc directly in the miRNA-target DEG network. EGF and Myc may exhibit significant roles in the progression of prostate cancer via regulation of the actin cytoskeleton and the MAPK signaling pathway. CHRM3 may activate these two pathways in prostate cancer progression. Thus, these two key factors and pathways may be crucial mechanisms during signal transduction by CHRM3 in prostate cancer. © Spandidos Publications 2015. All right reserved. Source


Mao G.,Peoples Care | Liu L.,The Fourth Peoples Hospital of Jinan
Experimental and Therapeutic Medicine | Year: 2014

The present study aimed to investigate the value of microRNA (miRNA)-18a for the early diagnosis of cerebral injury in patients with type 2 diabetes. Blood samples were collected from patients with type 2 diabetes, admitted to hospital between January and December 2013. The patients were randomly divided into three groups, which included one control and two experimental groups of severely and mildly diabetic patients (33 individuals per group). The levels of biochemical indicators in the serum, including S100 protein, neuron-specific enolase, myelin basic protein and endothelin-1, were determined. The mRNA and protein expression levels of hypoxia-inducible factor (HIF)-1α in the serum were measured by quantitative polymerase chain reaction (qPCR) and western blot analysis, respectively. In addition, the serum expression levels of miRNA-18a were determined by qPCR. The concentrations of the biochemical indicators in the severe diabetes group were significantly higher compared with those from the other two groups. Furthermore, the mRNA and protein expression levels of HIF-1α in the severe diabetes group were significantly upregulated compared with the other groups. However, the levels of miRNA-18a in the severe diabetes group were significantly downregulated compared with the other groups. The present study demonstrated that the elevation of biochemical indicators in the serum and the upregulation of HIF-1α mRNA and protein expression are associated with the downregulation of miRNA-18a. Therefore, miRNA-18a may be a potential genetic marker for the early diagnosis of cerebral injury induced by type 2 diabetes. © 2014, Spandidos Publications. All rights reserved. Source


Liu X.,Shandong University | Feng A.,The Fourth Peoples Hospital of Jinan | Cui Y.,Shandong University | Tobe R.G.,Shandong University
BioScience Trends | Year: 2013

Cervical cancer is the second leading cause of cancer deaths among women in the world and more than 85% of cervical cancer cases occur in women living in developing countries. Human papillomavirus (HPV) infection is the major cause of cervical cancer. Since 2006, two prophylactic vaccines against the high-risk strains of HPV have been developed and approved in more than 100 countries around the world. However, in China, HPV vaccines are still under clinical trials for government approval. In this paper feasibility and justification of HPV vaccine introduction into China is examined by reviewing experiences in both developed and developing countries where the vaccination program has been implemented. The vaccination program has showed significant cost-effectiveness and great health and economic impacts on cervical cancer prevention and control in both high-income and middle- and low-income countries. On the other hand, based on the lessons from both developed and developing countries, secondary prevention alone cannot fully play a role to reduce the incidence and the disease burden, and neither does the vaccination program. The epidemiological characteristics in China suggest an urgent need to introduce the vaccines and the geographically diversified prevalence of oncogenic HPV types as well as socioeconomic status also highlight the importance of region-driven approaches for cervical cancer prevention and control by integration of a screening and vaccination program. Source

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