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Lui N.-P.,Hong Kong Baptist University | Chen L.-W.,The Forth Military Medical University | Yung W.-H.,Chinese University of Hong Kong | Chan Y.-S.,University of Hong Kong | Yung K.K.-L.,Hong Kong Baptist University
PLoS ONE | Year: 2012

Here we report a previously unknown self repair mechanism during extremely early stages of rat Parkinsonism. Two important cell survival signaling cascades, Phosphatidylinositol-3 kinases (PI3K)/Akt pathway and extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway, could be responsible for this potential endogenous rescue system. In the 6-hydroxydopamine-lesioned rat, the phosphorylated p44/42 MAPK and its downstream target, the phosphorylated Bad at Ser 112, were up-regulated at post-lesion day 3 and lasted for a couple of weeks. Although the change in the phosphorylated Akt kinase was negligible throughout the studied period, its downstream target, the phosphorylated Bad at 136, was increased from post-lesion day 3 to post-lesion day 14. In the mean time, nestin-positive reactive astrocytes with low levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) appeared at post-lesion day 3 in 6-hydroxydopamine-lesioned rat. BDNF was expressed in both striatum and substantia nigra whereas GDNF was displayed in striatum only. At post-lesion day 14, nestin, BDNF and GDNF expressions were diminished. These neurotrophic factors were believed to initiate the above anti-apoptotic signal transduction cascades as we could see that their expression patterns were similar. The data strongly suggest that there is an endogenous repair effort by evoking the cell survival signaling and possibly via the releases of BDNF and GDNF from nestin-immunoreactive reactive astrocytes. ERK/MAPK pathway was proposed to be the key endogenous neuroprotective mechanisms, particularly in early stages of rat Parkinsonism. However, the self repair effort is only functional within an extremely short time window immediately after onset. © 2012 Lui et al. Source


Ma R.N.,The Forth Military Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2010

To evaluate the immunogenicity of the decellularized laryngeal scaffold. Twelve perfused, decellularized laryngeal scaffolds were obtained from rabbits through common carotid artery perfusion with detergents. The twelve decellularized laryngeal scaffolds and the twelve fresh larynxes were then implanted in para-laryngeal muscles of rabbits and harvested after two weeks, four weeks, twelve weeks and twenty-four weeks, respectively. Macroscopic view, histological examination and lymphocyte infiltration test were performed. The decellularized larynxes were implanted and preserved the laryngeal extracellular matrix and laryngeal architecture. The decellularized larynx did not show obvious immunological rejection after implanted into the para-laryngeal muscles of the recipient rabbits. The volume of implanted larynx became smaller but retained cartilage scaffold. The larynxes in the control group presented the serious immunological rejection and the majority tissues of the larynxes were disintegrated and substituted by the fibrous connective tissues after four weeks. The peripheral tissues were damaged and necrotic at different degrees. The quantity of the lymphocyte infiltration in the control group was higher than that in the experiment group and the result had the statistical significance (P < 0.01). Perfused, decellularized technique can construct a low immune laryngeal cartilage scaffold which could be a satisfactory material for laryngeal repair. Source


Zhu J.,Shaanxi University of Science and Technology | Zhang G.,Shaanxi University of Science and Technology | Miao Z.,The Forth Military Medical University | Shang T.,Shaanxi University of Science and Technology
Colloids and Surfaces A: Physicochemical and Engineering Aspects | Year: 2012

A novel comblike polymer, amphoteric polycarboxylic (AmPC), was designed as a dispersant for coal-water slurry (CWS) and synthesized in aqueous solution from the copolymerization of macromonomer polyethylene glycol-acrylate monoester (PA), sodium p-styrene sulfonate (SSS) and cationic comonomer methacrylatoethyl trimethyl ammonium chloride (DMC). PA was prepared by esterifying with polyethylene glycol (PEG) and acrylic acid (AA) in our laboratory. And then, the molecule structure of amphoteric polycarboxylic dispersant was characterized by means of Fourier transformer infrared (FTIR). Besides, the dispersant was applied in Shenfu coal slurry. By examining the apparent viscosity of the coal slurry using rheometer, the effects of the mole ratio of SSS and PA, amount of cationic monomer DMC, initiator concentration and reaction temperature on the AmPC dispersant performance were discussed. The wetting property of AmPC dispersant on the coal surface and Zeta potential of the CWS with AmPC dispersant was measured. The result shows that the apparent viscosity decrease of amphoteric polycarboxylic dispersant is better than the dispersant in stock which does not have cationic monomer when the dosage of DMC is 5.0. wt%. It has been proved that the amphoteric polycarboxylic dispersion agent is suitable for Shenfu coal slurry. When the dosage of AmPC dispersant is up to 0.3. wt%, the highest concentration of coal-slurry can reach 65.0. wt%. AmPC dispersant with both anionic group and cationic group will provide the better anchoring action with the coal via the adsorption of ion pair. It possesses not only dramatic wetting on coal but also stable steric hindrance so as to disperse coal particles in water. © 2012 Elsevier B.V. Source


Wang B.,The Forth Military Medical University | Shen C.,Changan University | Zhang L.,The Forth Military Medical University | Qi L.,The Forth Military Medical University | And 5 more authors.
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2015

Purpose: Our purpose was to explore pupil light response (PLR) with respect to the change in sensitivity of photoreceptors during various dark adaptation phases and to determine the optimal duration of dark adaptation time before the PLR. Methods: The PLR was recorded in 15 healthy subjects and three patients with neural or retinal vision loss after 1-sec blue and red light stimuli of 1, 10, and 100 cd/m2. The PLR was repeated nine times at different checkpoints during the 40-minute dark adaptation. The transient contraction amplitude, sustained contraction amplitude, and relative sustained contraction ratio of the PLR were analyzed. Results: The increase in the transient contraction amplitude during the entire dark adaptation process was significant (changing up to 45.1 %) in the initial phase of dark adaptation under different stimulus conditions. The changes in the sustained contraction amplitude and the relative sustained contraction ratio were substantial (changing up to 71.0 % and 37.2 % from 1 to 20 minutes of dark adaptation, respectively) under high-intensity blue illumination. The inflection point of the contraction curves in the dark adaptation was 15 or 20 minutes. The patients’ PLR results changed in a similar manner. Conclusions: The changes in the sensitivity of different photoreceptors occurred at different rates, and the contraction amplitude of the PLR was significantly affected by the dark adaptation duration. So 20 minutes of dark adaptation before PLR testing was suggested to achieve a consistent and stable pupil response. The dark adaptation effect should be put into consideration when comparing the results from different phases of the PLR test. © 2015, Springer-Verlag Berlin Heidelberg. Source


Tu Y.,The Forth Military Medical University | Lu J.,The Forth Military Medical University | Fu J.,Shanxi Medical University | Cao Y.,The Forth Military Medical University | And 4 more authors.
Japanese Journal of Clinical Oncology | Year: 2010

Objective: Neuroepithelial-transforming protein 1 is a member of the guanine nucleotide exchange factor family, a group of proteins which are known to activate and thereby regulate Rho family members. Deregulation of neuroepithelial-transforming protein 1 expression has been found in certain types of human tumors. To investigate its prognostic value in human gliomas, which is currently unknown, we examined the correlation between neuroepithelialtransforming protein 1 expression and prognosis in patients with gliomas. Methods: Immunohistochemical staining was performed to detect neuroepithelial-transforming protein 1 expression patterns in the biopsies from 96 patients with primary gliomas. Kaplan-Meier survival and Cox's regression analyses were performed to evaluate the prognosis of patients. Results: Immunohistochemical analysis with anti-neuroepithelial-transforming protein 1 antibody revealed that neuroepithelial-transforming protein 1 was significantly associated with the Karnofsky performance scale score and World Health Organization grades of patients with gliomas. Especially, the positive expression rates of neuroepithelial-transforming protein 1 were significantly higher in patients with higher grade (P = 0.001) and lower Karnofsky's performance scale score (P = 0.005). The median survival of patients with high neuroepithelialtransforming protein 1 expression was significantly shorter than that with low expression and without expression (316, 892 and 1180 days, respectively). Cox's multifactor analysis showed that the Karnofsky performance scale (P = 0.01), World Health Organization grade (P = 0.008) and neuroepithelial-transforming protein 1 (P = 0.006) were independent prognosis factors for human glioma. Conclusions: Taken together, our study indicates for the first time that neuroepithelial-transforming protein 1 status may be a highly sensitive marker for glioma prognosis and suggest that the expression patterns of neuroepithelial-transforming protein 1 might be a potent tool for predicting the clinical prognosis of glioma patients. © The Author (2010). Published by Oxford University Press. All rights reserved. Source

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