The First Peoples Hospital of Yuhang District

Hangzhou, China

The First Peoples Hospital of Yuhang District

Hangzhou, China
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Lu W.-L.,Chinese Academy of Sciences | Li J.-M.,Chinese Academy of Sciences | Yang J.,Chinese Academy of Sciences | Xu C.-G.,The First Peoples Hospital Of Huzhou | And 4 more authors.
Chinese Journal of Integrative Medicine | Year: 2017

Objective: To study the effects of Astragalus polysaccharide (APS), the primary effective component of the Chinese herb medicine Astragalus membranaceus (frequently used for its anti-hepatic fibrosis effects), on nanoscale mechanical properties of liver sinusoidal endothelial cells (SECs). Methods: Using endothelial cell medium as the control, 5 experimental groups were established utilizing different concentrations of APS, i.e. 12.5, 25, 50, 100, and 200 μg/mL. By using atomic force microscopy along with a microcantilever modified with a silicon dioxide microsphere as powerful tools, the value of Young’s modulus in each group was calculated. SAS 9.1 software was applied to analyze the values of Young’s modulus at the pressed depth of 300 nm. Environmental scanning electron microscopy was performed to observe the surface microtopography of the SECs. Results: The value of Young’s modulus in each APS experimental group was significantly greater than that of the control group: as APS concentration increased, the value of Young’s modulus presented as an increasing trend. The difference between the low-concentration (12.5 and 25 μg/mL) and high-concentration (200 μg/mL) groups was statistically signifificant (P<0.05), but no significant differences were observed between moderateconcentration (50 and 100 μg/mL) groups versus low- or high-concentration groups (P>0.05). Surface topography demonstrated that APS was capable of increasing the total area of fenestrae. Conclusions: The values of Young’s modulus increased along with increasing concentrations of APS, suggesting that the stiffness of SECs increases gradually as a function of APS concentration. The observed changes in SEC mechanical properties may provide a new avenue for mechanistic research of anti-hepatic fibrosis treatments in Chinese medicine. © 2017 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag GmbH Germany


Lou Z.,Zhejiang University | Yu X.,Shaoxing Shangyu Peoples Hospital | Li Y.,Zhejiang University | Duan H.,Zhejiang University | And 2 more authors.
Journal of Voice | Year: 2017

Objective: This study aimed to investigate the risk factors for postoperative arytenoid dislocation caused by endotracheal intubation. Methods: From September 2014 to September 2016, the records of 28 patients with a history of postoperative arytenoid dislocation were reviewed. Patients matched in type of anesthesia and surgery were chosen as the control (n = 56). Recorded data for all patients were demographics, smoking status, alcoholic status, operation time, and anesthesia procedures. For arytenoid dislocation cases, we further analyzed the incidences of the left and right arytenoid dislocations. Categorical variables were presented as frequencies and percentages, and were compared using the chi-square test. Continuous variables were expressed as means ± standard deviation and compared using the Student unpaired t test. To determine the predictors of arytenoid dislocation, a logistic regression model was used for multivariate analysis. Statistical significance was indicated by P < 0.05. Results: Twenty-eight patients demonstrating postoperative arytenoid dislocation (10 women and 18 men) were included, with a mean age of 55 ± 12 years. Sixteen patients (57.14%) had left arytenoid dislocation and 12 (42.86%) had right arytenoid dislocation. Univariate analysis indicated that body mass index (BMI) was associated with arytenoid dislocation (P < 0.01), and logistic regression analysis showed that BMI (P = 0.025) was an independent risk factor for postoperative arytenoid dislocation. Conclusions: BMI might be the independent risk factor for postoperative arytenoid dislocation. © 2017 The Voice Foundation.


Shi D.,The First Peoples Hospital of Yuhang District | Wang Q.-Z.,The Third Affiliated Hospital of Xin Xiang Medical College
Clinics and Research in Hepatology and Gastroenterology | Year: 2012

Objective: The aim of the study was to compare the cost-effectiveness, efficacy and safety of using a reloading multiband ligator with a neotype conductor (SD-c) with using a disposable one for endoscopic variceal ligation (EVL) over subsequent sessions. Methods: Patients undergoing variceal ligation over subsequent sessions were randomly subjected to EVL using a reloading multiband ligator with an SD-c (reloading group) or a disposable ligator (control group). The cost, efficacy and safety were analyzed and compared between the two groups. Results: A total of 460 patients underwent at least one session of EVL. Variceal obliteration was achieved in 124 patients (69%) in the reloading group and in 130 patients (70%) in the control group. The number of cases in which an extra band was released during deployment was three in the reloading group and two in the controls. Acute fever was seen in 38 cases after EVL in the reloading group and in 40 cases in the controls. In the reloading group, acute variceal bleeding events within the first 24. h after EVL were seen in three out of 327 (0.92%) patients versus six out of 335 (1.79%) in the control group. However, there were no significant differences between the two groups (P> 0.05). The cost savings were 2350. yuan/$369.89 for one session and 4277. yuan/$673.19 per patient on average. Conclusion: Although there were no statistically significant differences in efficacy or safety between the two patient groups, using a reloading multiband ligator for EVL is nevertheless a cost-savings procedure. © 2012 Elsevier Masson SAS.


Sun J.D.,The First Peoples Hospital Of Yuhang District | Yuan H.,The First Peoples Hospital Of Yuhang District | Hu H.Q.,The First Peoples Hospital Of Yuhang District | Yu H.M.,The First Peoples Hospital Of Yuhang District
Genetics and Molecular Research | Year: 2016

We investigated the possible correlations between N-acetyltransferase-2 (NAT2) gene polymorphisms and the risk of coronary heart disease (CHD). CHD patients (113) and healthy controls (118) were enrolled from the First People’s Hospital of Yuhang between January 2013 and June 2014. The patients were divided into mild CHD (N = 72) and severe CHD (N = 41) subgroups. DNA samples were extracted and the distributions of NAT2 polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Clinical characteristic indexes of severe CHD patients were also examined for relevant statistical analysis. WT, M1, M2, and M3 alleles were observed in both case and control groups. PCR-RFLP identified a wild-type homozygote, WT/WT; a mutant heterozygote, WT/Mx; and a mutant homozygote, Mx/Mx (x = 1, 2, and 3) variant of the NAT2 genotype. Mx/Mx differed significantly between case and control groups (P < 0.05); the frequencies of all four alleles did not differ significantly between case and control groups (P > 0.05). Slow acetylator genotype frequencies were notably higher in the case group than in the control group (P < 0.05). Individuals with the slow acetylator genotype were at 1.97-times higher risk of CHD and also displayed higher triglyceride and lower high-density lipoprotein cholesterol levels than those with the rapid acetylator genotype (P < 0.05). Therefore, the NAT2 polymorphism was believed to be associated with increased risk of CHD, with the NAT2 slow acetylator genotype serving as a risk factor for severe CHD in a Chinese population. © FUNPEC-RP.


Huang Y.,The First Peoples Hospital of Yuhang District | Ren G.-P.,Zhejiang University | Xu C.,The First Peoples Hospital of Yuhang District | Dong S.-F.,The First Peoples Hospital of Yuhang District | And 4 more authors.
BMC Cancer | Year: 2014

Background: Altered expression of astrocyte elevated gene-1 (AEG-1) is associated with tumorigenesis and progression. The present study aimed to investigate the clinical and prognostic significance of AEG-1 expression in pancreatic ductal adenocarcinoma (PDAC).Methods: Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot analyses were employed to assess AEG-1 expression in three pancreatic cancer cell lines and normal pancreatic duct epithelial cells. qRT-PCR and immunohistochemical analyses were performed to detect AEG-1 expression in ten pairs of PDAC and normal pancreas tissues. Immunohistochemistry was then used to examine AEG-1 expression in paraffin-embedded tissues obtained from 105 patients, and its association with clinicopathological parameters including cancer classification was examined. Kaplan-Meier analysis was performed to study the survival rates of patients.Results: Expression of AEG-1 mRNA and protein was markedly higher in pancreatic cancer cell lines than that in the normal pancreatic duct epithelial cells. AEG-1 expression was evidently upregulated in PDAC tissues compared to that of the matched distant normal pancreas tissues. qRT-PCR data revealed that the tumor/non-tumor ratio of AEG-1 expression was >1.5-fold (up to 6.5-fold). Immunohistochemical data showed that AEG-1 protein was detected in 98.09% (103/105) of PDAC tissues; and they were found to be associated with tumor size (P = 0.025), advanced clinical stage (P = 0.004), T classification (P = 0.006), N classification (P = 0.003), and M classification (P = 0.007). Furthermore, Kaplan-Meier analysis showed that patients with high AEG-1-expressed PDAC had shorter overall survival. A multivariate Cox regression analysis revealed that clinical stage, T classification, and AEG-1 expression were the independent prognostic predictors for PDAC.Conclusions: This study suggests that AEG-1 protein was highly expressed in PDAC and associated with poor prognosis of the patients. © 2014 Huang et al.; licensee BioMed Central Ltd.


PubMed | the First Peoples Hospital of Yuhang District
Type: Journal Article | Journal: Clinics and research in hepatology and gastroenterology | Year: 2012

The aim of the study was to compare the cost-effectiveness, efficacy and safety of using a reloading multiband ligator with a neotype conductor (SD-c) with using a disposable one for endoscopic variceal ligation (EVL) over subsequent sessions.Patients undergoing variceal ligation over subsequent sessions were randomly subjected to EVL using a reloading multiband ligator with an SD-c (reloading group) or a disposable ligator (control group). The cost, efficacy and safety were analyzed and compared between the two groups.A total of 460 patients underwent at least one session of EVL. Variceal obliteration was achieved in 124 patients (69%) in the reloading group and in 130 patients (70%) in the control group. The number of cases in which an extra band was released during deployment was three in the reloading group and two in the controls. Acute fever was seen in 38 cases after EVL in the reloading group and in 40 cases in the controls. In the reloading group, acute variceal bleeding events within the first 24h after EVL were seen in three out of 327 (0.92%) patients versus six out of 335 (1.79%) in the control group. However, there were no significant differences between the two groups (P>0.05). The cost savings were 2350 yuan/$369.89 for one session and 4277 yuan/$673.19 per patient on average.Although there were no statistically significant differences in efficacy or safety between the two patient groups, using a reloading multiband ligator for EVL is nevertheless a cost-savings procedure.


PubMed | The First Peoples Hospital of Yuhang District
Type: | Journal: BMC cancer | Year: 2014

Altered expression of astrocyte elevated gene-1 (AEG-1) is associated with tumorigenesis and progression. The present study aimed to investigate the clinical and prognostic significance of AEG-1 expression in pancreatic ductal adenocarcinoma (PDAC).Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot analyses were employed to assess AEG-1 expression in three pancreatic cancer cell lines and normal pancreatic duct epithelial cells. qRT-PCR and immunohistochemical analyses were performed to detect AEG-1 expression in ten pairs of PDAC and normal pancreas tissues. Immunohistochemistry was then used to examine AEG-1 expression in paraffin-embedded tissues obtained from 105 patients, and its association with clinicopathological parameters including cancer classification was examined. Kaplan-Meier analysis was performed to study the survival rates of patients.Expression of AEG-1 mRNA and protein was markedly higher in pancreatic cancer cell lines than that in the normal pancreatic duct epithelial cells. AEG-1 expression was evidently upregulated in PDAC tissues compared to that of the matched distant normal pancreas tissues. qRT-PCR data revealed that the tumor/non-tumor ratio of AEG-1 expression was >1.5-fold (up to 6.5-fold). Immunohistochemical data showed that AEG-1 protein was detected in 98.09% (103/105) of PDAC tissues; and they were found to be associated with tumor size (P = 0.025), advanced clinical stage (P = 0.004), T classification (P = 0.006), N classification (P = 0.003), and M classification (P = 0.007). Furthermore, Kaplan-Meier analysis showed that patients with high AEG-1-expressed PDAC had shorter overall survival. A multivariate Cox regression analysis revealed that clinical stage, T classification, and AEG-1 expression were the independent prognostic predictors for PDAC.This study suggests that AEG-1 protein was highly expressed in PDAC and associated with poor prognosis of the patients.


PubMed | The First Peoples Hospital of Yuhang District
Type: Comparative Study | Journal: Journal of laparoendoscopic & advanced surgical techniques. Part A | Year: 2014

Large common bile duct (CBD) stones remain a major challenge for endoscopists. The capture of large CBD stones is a limiting factor for successful removal. The aim of the study is to evaluate the efficacy and safety of stretching the basket out at the bottom of the stones to capture large CBD stones.Sixty-five patients with large stones (>20mm in diameter) were selected and randomly divided into Group A (33 cases) and Group B (34 cases). After appropriate sphincterotomy with balloon dilation, two different methods were used to capture stones. For Method A, the basket was inserted above a large stone and opened to capture it. For Method B, the basket was stretched out at the bottom of the large stone and opened slowly to capture it.The success rate of capturing stones was 33.3% in Group A and 94.1% in Group B (P<.05). There was no statistically significant difference in complication rate between the two groups (P>.05).The success rate for capturing large CBD stones can be increased by stretching the basket out at the bottom of the stones to capture the stone.


PubMed | The First Peoples Hospital of Yuhang District
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

We investigated the possible correlations between N-acetyltransferase-2 (NAT2) gene polymorphisms and the risk of coronary heart disease (CHD). CHD patients (113) and healthy controls (118) were enrolled from the First Peoples Hospital of Yuhang between January 2013 and June 2014. The patients were divided into mild CHD (N = 72) and severe CHD (N = 41) subgroups. DNA samples were extracted and the distributions of NAT2 polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Clinical characteristic indexes of severe CHD patients were also examined for relevant statistical analysis. WT, M1, M2, and M3 alleles were observed in both case and control groups. PCR-RFLP identified a wild-type homozygote, WT/WT; a mutant heterozygote, WT/Mx; and a mutant homozygote, Mx/Mx (x = 1, 2, and 3) variant of the NAT2 genotype. Mx/Mx differed significantly between case and control groups (P < 0.05); the frequencies of all four alleles did not differ significantly between case and control groups (P > 0.05). Slow acetylator genotype frequencies were notably higher in the case group than in the control group (P < 0.05). Individuals with the slow acetylator genotype were at 1.97-times higher risk of CHD and also displayed higher triglyceride and lower high-density lipoprotein cholesterol levels than those with the rapid acetylator genotype (P < 0.05). Therefore, the NAT2 polymorphism was believed to be associated with increased risk of CHD, with the NAT2 slow acetylator genotype serving as a risk factor for severe CHD in a Chinese population.


PubMed | The First Peoples Hospital of Yuhang District
Type: Journal Article | Journal: Future oncology (London, England) | Year: 2012

To investigate the effects of the expression of the MUC5AC protein in gastric cancer depending on the Helicobacter pylori (Hp) infection status.The MUC5AC protein and mRNA were detected using western blot and real-time PCR protocols in gastric cancer tissue and stratified for Hp infection. Gastric mucus membranes near the cancer site serve as the control group.The expression of MUC5AC protein and mRNA is significantly decreased in gastric cancer tissue (p < 0.05). The decrease was more significant in the Hp-infected group than in the Hp-uninfected group (p < 0.05).The infection of Hp is correlated with a decrease in MUC5AC protein amount in gastric cancer tissue. The current result suggests that there may be a potential necessary link between Hp, MUC5AC and gastric cancer.

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