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Cao Z.-G.,Sihong County Peoples Hospital | Qin X.-B.,The First Peoples Hospital Of Xuzhou | Liu F.-F.,Huaian Second Peoples Hospital | Zhou L.-L.,Nanjing Medical University
African Health Sciences | Year: 2015

Background: The pathogenesis of breast cancer remains unclear. Aims: To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer. Methods: Patients with breast cancers were enrolled in our hospital between year January 1st, 2013 and December 31st, 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell. Results: Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration. Conclusion: The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer. © 2015, Makerere University, Medical School. All rights reserved.


Wang W.,The First Peoples Hospital Of Xuzhou
Experimental and Therapeutic Medicine | Year: 2016

Polyacrylamide hydrogel (PAHG) is a medical soft tissue filling agent utilized for the treatment of a variety of soft tissue defects. However, postoperative complications occur that have to be alleviated. The aim of the present study was to examine the treatments of postoperative complications of mammoplasty augmentation with PAHG. Open suction techniques and partial mastectomies via periareolar incisions were performed in 28 patients who had been injected with PAHG for breast augmentation. The PAHG was removed precluding breast complications resulting from the hydrogel injections. The symptoms disappeared completely, and the results were satisfactory following removal of the PAHG. In conclusion, the periareolar approach is valuable and removes PAHG to the greatest extent. © 2016, Spandidos Publications. All rights reserved.


Liu N.,The First Peoples Hospital Of Xuzhou | Sun B.,The First Peoples Hospital Of Xuzhou | Wu P.,The First Peoples Hospital Of Xuzhou | Wei X.,The First Peoples Hospital Of Xuzhou
Cell Biochemistry and Biophysics | Year: 2015

The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A. indica treated, and DMH+AI treated. Initiation and induction of colon carcinogenesis were achieved through weekly subcutaneous injections of DMH (30 mg/kg body weight) for both 10 and 20 weeks. A. indica extract was supplemented to rats at a dose rate of 100 mg/kg body weight of animals thrice a week on alternative days, ad libitum for two different time durations of 10 and 20 weeks. The study observed a significant increase in the number of aberrant crypt foci in colons of DMH-treated rats at both the time intervals which were decreased significantly upon AI supplementation. Also, a significant increase was seen in the enzyme activity of alkaline phosphatase, which, however, was moderated upon AI administration to DMH-treated rats. Changes in the ultrastructural architecture of colonic cells were apparent following both the treatment schedules of DMH; however, the changes were prominent following 20 weeks of DMH treatment. The most obvious changes were seen in the form of altered nuclear shape and disruption of cellular integrity, which were appreciably improved upon AI supplementation. In conclusion, the study shows the chemopreventive abilities of AI against DMH-induced colorectal carcinogenesis in rats. © 2015, Springer Science+Business Media New York.


Sun B.,The First Peoples Hospital Of Xuzhou | Li W.,The First Peoples Hospital Of Xuzhou | Liu N.,The First Peoples Hospital Of Xuzhou
Oncology Letters | Year: 2016

Advanced colorectal cancer has a high mortality rate and conventional treatments have poor therapeutic effects. The aim of the present study was to analyze the recent curative effect and adverse reaction of photofrin photodynamic adjuvant treatment on young patients with advanced colorectal cancer. A total of 23 patients with advanced colorectal cancer who had accepted semiconductor laser photodynamic adjuvant treatment were selected as the observation group. In addition, 30 patients who had accepted concurrent radiotherapy and chemotherapy during the same period served as the control group. The observation group received photofrin (2 mg/kg) intravenously in 100 ml of 5% glucose, followed by the introduction of the endoscopic optical fiber to deliver laser radiation with an intensity of 630 nm wavelength pulse power. After 2 days, necrotic tissues were removed and irradiation of the original or new tumor lesions was performed and necrotic tissues were removed. The total effective rate and survival time was higher and the length of hospital stay was shorter in the observation group in comparison with the control group. The differences were statistically significant (P<0.05). The number of patients in the control and observation groups with symptoms of hematochezia, change in bowel habit, intestinal stimulation and incomplete intestinal obstruction were reduced. Additionally, the reduced ratio of the observation group was significantly increased in comparison with the control group (P<0.05). The adverse reaction rate of the observation group was lower than that of the control group and this difference was also statistically significant (P<0.05). In conclusion, use of photodynamic treatment for young patients with advanced colorectal cancer can effectively improve the clinical symptoms and reduce complications. © Spandidos Publications 2016.


Fan S.-H.,Jiangsu University | Wang Y.-Y.,The First Peoples Hospital of Xuzhou | Wu Z.-Y.,Fudan University | Zhang Z.-F.,Jiangsu University | And 7 more authors.
Oncotarget | Year: 2015

Human 1-acylglycerol-3-phosphate O-acyltransferase 9 (AGPAT9) is the gene identified from adipose tissue in 2007. We found AGPAT9 expression was significantly higher in poorly invasive MCF7 human breast cancer cells than the highly invasive MDA-MB-231 cells. AGPAT9 significantly inhibited the proliferation of breast cancer cells in vitro and in vivo. Live-cell imaging and transwell assays showed that AGPAT9 could significantly inhibit the migration and invasive capacities of breast cancer cells. The inhibitory effect of AGPAT9 on metastasis was also observed in vivo in lung metastasis model. AGPAT9 inhibited breast cancer cell proliferation, migration and invasion through, at least in part, suppressing the V-ATPase activity. In addition, increased AGPAT9 expression in MCF-7/ADR cells could increase the chemosensitivity to doxorubicin (Dox). Our findings suggest that increasing AGPAT9 expression may be a new approach that can be used for breast cancer treatment.


Gao L.-Y.,The First Peoples Hospital of Xuzhou | Ding J.,The First Peoples Hospital of Xuzhou | Zhao L.,The First Peoples Hospital of Xuzhou
Experimental and Therapeutic Medicine | Year: 2016

Weneger's granulomatosis (WG) is a rare autoimmune disease affecting a number of organs, including lungs and kidney. Although all age groups may be affected, the peak incidence occurs for individuals aged 30-50 years, with a slightly increased prevalence in males. In the current study, we present three cases of WG to describe clinical and pathological characteristics of this disease. Three patients with WG were assessed for clinical and pathological characteristics, and typical morphological findings using computerized tomography. Clinical manifestations included vasculitis, tissue necrosis, and the formation of granuloma. Following a large dose of glucocorticoid hormone treatment, the symptoms were successfully relieved in all three patients. In conclusion, the diagnosis of WG is dependent on patho- logical examination in combination with appropriate clinical and imaging data, and immunostaining with anti-neutrophil cytoplasmic antibody. © 2016, Spandidos Publications. All rights reserved.


PubMed | Sihong County Peoples Hospital, The First Peoples Hospital of Xuzhou, Huaian Second Peoples Hospital and Nanjing Medical University
Type: Journal Article | Journal: African health sciences | Year: 2016

The pathogenesis of breast cancer remains unclear.To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer.Patients with breast cancers were enrolled in our hospital between year January 1(st), 2013 and December 31(st), 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration.The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.


PubMed | The First Peoples Hospital of Xuzhou
Type: Case Reports | Journal: Cell biochemistry and biophysics | Year: 2016

Confocal laser scanning microscopy (CLSM) is a novel non-invasive imaging technique for in vivo evaluation of cutaneous lesions at near-histologic resolution. The applicability of CLSM for various neoplastic and inflammatory skin diseases has been shown. The objective of the study is to utilize the CLSM for the differential diagnosis of atypical dermatoses. Six patients with atypical clinical manifestation were detected by CLSM. In spite of non-typical clinical manifestations, CLSM can still detect their characteristic pathological changes and help differentiate them from other diseases that are liable to be confused in clinical practice. CLSM deserves wide application in clinical practice as it boasts of easy and convenient operation, broad application, no pains or traumas for patients, rapid examination reports, as well as it can relieve patients distress by avoiding the traumas resulting from histopathological biopsy.


PubMed | The First Peoples Hospital of Xuzhou
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A. indica treated, and DMH+AI treated. Initiation and induction of colon carcinogenesis were achieved through weekly subcutaneous injections of DMH (30mg/kg body weight) for both 10 and 20weeks. A. indica extract was supplemented to rats at a dose rate of 100mg/kg body weight of animals thrice a week on alternative days, ad libitum for two different time durations of 10 and 20weeks. The study observed a significant increase in the number of aberrant crypt foci in colons of DMH-treated rats at both the time intervals which were decreased significantly upon AI supplementation. Also, a significant increase was seen in the enzyme activity of alkaline phosphatase, which, however, was moderated upon AI administration to DMH-treated rats. Changes in the ultrastructural architecture of colonic cells were apparent following both the treatment schedules of DMH; however, the changes were prominent following 20weeks of DMH treatment. The most obvious changes were seen in the form of altered nuclear shape and disruption of cellular integrity, which were appreciably improved upon AI supplementation. In conclusion, the study shows the chemopreventive abilities of AI against DMH-induced colorectal carcinogenesis in rats.


PubMed | the First Peoples Hospital of Xuzhou
Type: Journal Article | Journal: European review for medical and pharmacological sciences | Year: 2016

We investigated the effects of small interference RNA (siRNA) on the cell proliferation inhibition, sensitivity to radiotherapy effects and cell apoptosis. The siRNA used here was specific to the pituitary tumor transforming gene (PTTG).Vectors containing the specific functional siRNAs for PTTG were designed and constructed. Cells were divided into four groups: (I) blank control group; (II) radiotherapy group: cells were exposed to X-ray radiation; (III) Group PTTG siRNA: transfected with PTTG siRNA; (IV) PTTG siRNA+ radiotherapy group: transfected with PTTG siRNA and then were exposed to X-ray radiation. HEC-1A cells were transfected by the specific interfering plasmids using Lipofectamine 2000 transfection reagent. The PTTG protein expression levels were analyzed using Western blot Cell proliferation was examined by MTT assay and the HEC-1A cell line apoptosis was evaluated by flow cytometry.Recombinant small interference RNA (siRNA) expression vectors targeting PTTG were successfully constructed. The results of MTT showed that the growth of the HEC-1A cell was negatively influenced after cells were transfected with PTTG siRNA. Furthermore, PTTG siRNA combined with radiotherapy demonstrated more powerful inhibitory effects. Cell apoptosis rates were significantly increased in the radiotherapy group and the PTTG siRNA transfection group when compared to the control group. A more pronounced cell apoptosis rate was observed in the group that was treated with PTTG siRNA combined with radiotherapy.Recombinant small interference RNA (siRNA) expression vector targeting PTTG successfully inhibited the cell proliferation and induced apoptosis in endometrial carcinoma cells and increased the cancer cells vulnerability to the effects of radiation.

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