The First Peoples Hospital of Xuzhou
The First Peoples Hospital of Xuzhou
Ma X.-D.,The First Peoples Hospital of Xuzhou |
Li B.-P.,The First Peoples Hospital of Xuzhou |
Wang D.-L.,The First Peoples Hospital of Xuzhou |
Yang W.-S.,The First Peoples Hospital of Xuzhou
Experimental and Therapeutic Medicine | Year: 2017
The present study determined the effect of dexmedetomidine (Dex) combined with flurbiprofen axetil (FA) on analgesia, immune response, and preservation of cognitive function in patients subjected to general anesthesia. We recruited 100 patients with thyroid surgery and randomly divided them into four groups: Dex (D), FA (F), Dex combined with FA (DF), and saline control (C). The extubation and recovery times for Groups D and DF were significantly longer than for Groups F and C. After extubation, the heart rate and mean arterial pressure for Groups F, D, and DF were significantly lower than for Group C, and data for Group DF was significantly lower than for Group F. The visual analog scale and Riker sedation agitation scores were significantly lower in Group DF than for the other three groups. T- and B-lymphocytes were significantly higher in Group DF than in the other three groups. Compared with Groups F and C, the levels of TNF-α and IL-6 in Group DF were significantly reduced, while IL-2 markedly increased. The combined use of Dex and FA significantly improved pain after general anesthesia thyroid surgery, reduced restlessness and postoperative cognitive dysfunction, enhanced immune function, and promoted wound repair. © 2017, Spandidos Publications. All rights reserved.
Zhou H.,Nanjing Medical University |
Xu Q.,The Peoples Hospital Of Yang Zhong City |
Wang Z.-M.,The First Peoples Hospital Of Xuzhou |
Zou W.-W.,Huaian Second Peoples Hospital
Latin American Journal of Pharmacy | Year: 2015
Cytochrome P450 (CYP) isoforms metabolism plays a key role in the elimination of many clinical drugs, and CYPs-inhibition based drug-drug interaction remains to be the key limiting factor for the R&D of new chemical entities as drugs or the clinical application of clinical drugs. The present study aims to evaluate the drug-drug interaction between warfarin and gomisin J which is a promising drug for anti-ovarian cancer utilization. The crystal structure of CYP2C9 (protein code: 1OG2) was downloaded from protein database (http://www.rcsb.org/pdb). For this crystal structure, warfarin was bound with the active site as a good substrate. The missing residues in the protein chain were added through the processing of this protein. Advanced Chemistry Development (ACD) software was employed to draw the molecular structure of gomisin J. Gomisin J has close distance with the activity site, and the interaction between compound and active site was mainly the hydrogen interaction, and the interaction amino acids located in the CYP2C9 were Gly98 and Phe100 . The co-docking process of both warfarin and gomisin J into the active site of CYP2C9 was carried out, and gomisin J showed closer distance towards active center than warfarin, indicating that gomisin J might inhibit CYP2C9-catalyzed metabolism of many clinical drugs. © 2015, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Wu Y.,The First Peoples Hospital of Xuzhou
European review for medical and pharmacological sciences | Year: 2016
OBJECTIVE: In this study the levels of macrophage inflammatory protein-1α and NF-κB were measured in patients suffering acute kidney injury secondary to acute lung injury induced by mechanical ventilation and in controls, to determine whether they are differentially expressed.PATIENTS AND METHODS: 160 patients were enrolled in our study: 40 had acute kidney injury secondary to acute lung injury induced by mechanical ventilation (group A), 40 had acute lung injury induced by mechanical ventilation, but no secondary acute kidney injury (group B), 40 were treated with mechanical ventilation but suffered no complications (group C), and 40 were treated with a conventional nasal catheter or oxygen mask inhalation (group D). The seric levels of MIP-1α, NF-κB and hs-CRP were compared amongst the groups at time points of 6, 12, 24, and 72 hours and at 7 days after the start of the respiratory treatment.RESULTS: The serum levels of MIP-1α, NF-κB and hs-CRP of groups A and B were significantly higher than those of groups C and D at each time point. Also, group A had higher levels than group B at each time point, and the differences were statistically significant (p < 0.05). No statistically significant differences were found while comparing levels in group C with those of group D (p > 0.05). In groups A and B, the levels of MIP-1α increased gradually to a peak at 72 hours and then fell again on the 7th day. Levels of NF-κB in groups A and B significantly increased at 6, 12 and 24 hours, and reached a peak level at 24 h, to then fall after 72 h. The levels in group A fell back to baseline at 7 days, while group B levels fell back to baseline faster, at 72 h. Finally, the levels of hs-CRP in groups A and B kept increasing even after 7 days.CONCLUSIONS: Based on these results, it is possible that the levels of MIP-1α and NF-κB be used as early indicators of inflammation reflecting the occurrence of acute kidney injury secondary to acute lung injury induced by mechanical ventilation.
Cao Z.-G.,Sihong County Peoples Hospital |
Qin X.-B.,The First Peoples Hospital Of Xuzhou |
Liu F.-F.,Huaian Second Peoples Hospital |
Zhou L.-L.,Nanjing Medical University
African Health Sciences | Year: 2015
Background: The pathogenesis of breast cancer remains unclear. Aims: To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer. Methods: Patients with breast cancers were enrolled in our hospital between year January 1st, 2013 and December 31st, 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell. Results: Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration. Conclusion: The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer. © 2015, Makerere University, Medical School. All rights reserved.
Wang W.,The First Peoples Hospital Of Xuzhou
Experimental and Therapeutic Medicine | Year: 2016
Polyacrylamide hydrogel (PAHG) is a medical soft tissue filling agent utilized for the treatment of a variety of soft tissue defects. However, postoperative complications occur that have to be alleviated. The aim of the present study was to examine the treatments of postoperative complications of mammoplasty augmentation with PAHG. Open suction techniques and partial mastectomies via periareolar incisions were performed in 28 patients who had been injected with PAHG for breast augmentation. The PAHG was removed precluding breast complications resulting from the hydrogel injections. The symptoms disappeared completely, and the results were satisfactory following removal of the PAHG. In conclusion, the periareolar approach is valuable and removes PAHG to the greatest extent. © 2016, Spandidos Publications. All rights reserved.
Liu N.,The First Peoples Hospital Of Xuzhou |
Sun B.,The First Peoples Hospital Of Xuzhou |
Wu P.,The First Peoples Hospital Of Xuzhou |
Wei X.,The First Peoples Hospital Of Xuzhou
Cell Biochemistry and Biophysics | Year: 2015
The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A. indica treated, and DMH+AI treated. Initiation and induction of colon carcinogenesis were achieved through weekly subcutaneous injections of DMH (30 mg/kg body weight) for both 10 and 20 weeks. A. indica extract was supplemented to rats at a dose rate of 100 mg/kg body weight of animals thrice a week on alternative days, ad libitum for two different time durations of 10 and 20 weeks. The study observed a significant increase in the number of aberrant crypt foci in colons of DMH-treated rats at both the time intervals which were decreased significantly upon AI supplementation. Also, a significant increase was seen in the enzyme activity of alkaline phosphatase, which, however, was moderated upon AI administration to DMH-treated rats. Changes in the ultrastructural architecture of colonic cells were apparent following both the treatment schedules of DMH; however, the changes were prominent following 20 weeks of DMH treatment. The most obvious changes were seen in the form of altered nuclear shape and disruption of cellular integrity, which were appreciably improved upon AI supplementation. In conclusion, the study shows the chemopreventive abilities of AI against DMH-induced colorectal carcinogenesis in rats. © 2015, Springer Science+Business Media New York.
Sun B.,The First Peoples Hospital Of Xuzhou |
Li W.,The First Peoples Hospital Of Xuzhou |
Liu N.,The First Peoples Hospital Of Xuzhou
Oncology Letters | Year: 2016
Advanced colorectal cancer has a high mortality rate and conventional treatments have poor therapeutic effects. The aim of the present study was to analyze the recent curative effect and adverse reaction of photofrin photodynamic adjuvant treatment on young patients with advanced colorectal cancer. A total of 23 patients with advanced colorectal cancer who had accepted semiconductor laser photodynamic adjuvant treatment were selected as the observation group. In addition, 30 patients who had accepted concurrent radiotherapy and chemotherapy during the same period served as the control group. The observation group received photofrin (2 mg/kg) intravenously in 100 ml of 5% glucose, followed by the introduction of the endoscopic optical fiber to deliver laser radiation with an intensity of 630 nm wavelength pulse power. After 2 days, necrotic tissues were removed and irradiation of the original or new tumor lesions was performed and necrotic tissues were removed. The total effective rate and survival time was higher and the length of hospital stay was shorter in the observation group in comparison with the control group. The differences were statistically significant (P<0.05). The number of patients in the control and observation groups with symptoms of hematochezia, change in bowel habit, intestinal stimulation and incomplete intestinal obstruction were reduced. Additionally, the reduced ratio of the observation group was significantly increased in comparison with the control group (P<0.05). The adverse reaction rate of the observation group was lower than that of the control group and this difference was also statistically significant (P<0.05). In conclusion, use of photodynamic treatment for young patients with advanced colorectal cancer can effectively improve the clinical symptoms and reduce complications. © Spandidos Publications 2016.
Fan S.-H.,Jiangsu University |
Wang Y.-Y.,The First Peoples Hospital of Xuzhou |
Wu Z.-Y.,Fudan University |
Zhang Z.-F.,Jiangsu University |
And 7 more authors.
Oncotarget | Year: 2015
Human 1-acylglycerol-3-phosphate O-acyltransferase 9 (AGPAT9) is the gene identified from adipose tissue in 2007. We found AGPAT9 expression was significantly higher in poorly invasive MCF7 human breast cancer cells than the highly invasive MDA-MB-231 cells. AGPAT9 significantly inhibited the proliferation of breast cancer cells in vitro and in vivo. Live-cell imaging and transwell assays showed that AGPAT9 could significantly inhibit the migration and invasive capacities of breast cancer cells. The inhibitory effect of AGPAT9 on metastasis was also observed in vivo in lung metastasis model. AGPAT9 inhibited breast cancer cell proliferation, migration and invasion through, at least in part, suppressing the V-ATPase activity. In addition, increased AGPAT9 expression in MCF-7/ADR cells could increase the chemosensitivity to doxorubicin (Dox). Our findings suggest that increasing AGPAT9 expression may be a new approach that can be used for breast cancer treatment.
Gao L.-Y.,The First Peoples Hospital of Xuzhou |
Ding J.,The First Peoples Hospital of Xuzhou |
Zhao L.,The First Peoples Hospital of Xuzhou
Experimental and Therapeutic Medicine | Year: 2016
Weneger's granulomatosis (WG) is a rare autoimmune disease affecting a number of organs, including lungs and kidney. Although all age groups may be affected, the peak incidence occurs for individuals aged 30-50 years, with a slightly increased prevalence in males. In the current study, we present three cases of WG to describe clinical and pathological characteristics of this disease. Three patients with WG were assessed for clinical and pathological characteristics, and typical morphological findings using computerized tomography. Clinical manifestations included vasculitis, tissue necrosis, and the formation of granuloma. Following a large dose of glucocorticoid hormone treatment, the symptoms were successfully relieved in all three patients. In conclusion, the diagnosis of WG is dependent on patho- logical examination in combination with appropriate clinical and imaging data, and immunostaining with anti-neutrophil cytoplasmic antibody. © 2016, Spandidos Publications. All rights reserved.
PubMed | Sihong County Peoples Hospital, The First Peoples Hospital of Xuzhou, Huaian Second Peoples Hospital and Nanjing Medical University
Type: Journal Article | Journal: African health sciences | Year: 2016
The pathogenesis of breast cancer remains unclear.To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer.Patients with breast cancers were enrolled in our hospital between year January 1(st), 2013 and December 31(st), 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration.The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.