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Ouyang H.-C.,The First Peoples Hospital of Shunde District | Wu W.-D.,Guangzhou University | Zhong D.-M.,Guangzhou University of Chinese Medicine | Wu Y.-X.,The First Peoples Hospital of Shunde District | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: We have found that Xingnaojing can effectively inhibit mediators of inflammatory mediators and promote fibrinolytic activity in a rabbit model of myocardial ischemia and reperfusion. However, it is not completely clear what is the action of mechanism of fibrinolytic system activity in human umbilical vein endothelial cells. OBJECTIVE: To investigate the effects of Xingnaojing on recombinant human tumor necrosis factor α-mediated tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) expression in human umbilical cord vein endothelial cells. METHODS: The passages 3-5 of human umbilical cord vein endothelial cells were obtained, and were induced by adding 10 μg/L recombinant human tumor necrosis factor α in the culture medium. Xingnaojing in different concentrations (5, 10, 20 mL/L) were injected in the Xingnaojing group, and fluvastatin (1 μmol/L) was added as positive control group. A blank control group of simple human umbilical cord vein endothelial cells culture was set up. After 24 hours of cultivation, the production of t-PA and PAI-1 in the cultured medium was determined by ELISA. The mRNA expression of t-PA and PAI-1 was measured by reverse transcript-PCR. RESULTS AND CONCLUSION: Compared with the blank control group, the secretion and mRNA expression of PAI-1 in the group treated with recombinant human tumor necrosis factor α (10 μg/L) were significantly increased (P < 0.05); however, the secretion and mRNA expression of t-PA in the group treated with recombinant human tumor necrosis factor α (10 μg/L) were significantly decreased (P < 0.05). The secretion and mRNA expression of t-PA were significantly higher in the Xingnaojing group at different concentrations than in the recombinant human tumor necrosis factor α group (P < 0.05); however, the secretion and mRNA expression of PAI-1 were significantly lower in the former group than the latter group (P < 0.05), which was in a dose-dependent manner. Xingnaojing can effectively improve the fibrinolytic function of human umbilical cord vein endothelial cells mediated by recombinant human tumor necrosis factor α. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Li Z.-H.,The First Peoples Hospital of Shunde District | Duan Y.-B.,The First Peoples Hospital of Shunde District | Luo C.-Y.,The First Peoples Hospital of Shunde District | Tang X.-F.,The First Peoples Hospital of Shunde District | And 4 more authors.
International Eye Science | Year: 2013

AIM: To investigate the morbidity of dry eye in policeman of Shunde district, and to analyze the related factors and proposed methods of preventing and treating dry eye. METHODS: Totally 650 policemen who came for physical examination in Shunde district recived questionnaire survey. The patients with dye eye disease were finally diagnosed through slit lamp examination, Schirmer test, tear film break-up time (BUT) measurement and keratoconjunctival fluorescent staining. The morbidity among different policemen was further analyzed and stasticed. RESULTS: The results showed that 152 in 650 policemen suffered from dry eye disease, the ratio was 23.4%. Comparied with security police and crimanl police, the ratio in traffic police, patrolman, and back office police was significantly higher. The prevalence was higher when the ages were greater than 40. CONCLUSION: Unsuitable working environment, air contamination are high risk factors of dry eye disease. Source

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