The First Peoples Hospital of Shangqiu

Henan’an, China

The First Peoples Hospital of Shangqiu

Henan’an, China
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Qiu X.,The First Peoples Hospital of Shangqiu | Dou Y.,The First Peoples Hospital of Shangqiu
Biomedicine and Pharmacotherapy | Year: 2017

microRNAs have emerged as important regulators in various cancers, including prostate cancer. In this study, we investigated the role of miR-1307 in cell proliferation of prostate cancer. We found miR-1307 was overexpressed in prostate cancer cells and tissues, overexpression of miR-1307 significantly promoted cell proliferation and tumorigenesis in vitro investigated by MTT assay, colony formation assay and soft agar growth assay, meanwhile overexpression of miR-1307 inhibited cell cycle inhibitors p21 and p27 both in mRNA and protein levels. Knockdown of miR-1307 reduced these effects, confirming miR-1307 promotes prostate cancer cell proliferation. FOXO3A (Forkhead box protein O3a) was the target of miR-1307, miR-1307 directly bound to the 3′UTR of FOXO3A. Simultaneous knockdown of miR-1307 and FOXO3A promoted cell proliferation of prostate cancer. In summary, our results suggested miR-1307 contributed to prostate cancer proliferation by targeting FOXO3A. © 2016 Elsevier Masson SAS


Shan W.,The First Peoples Hospital Of Shangqiu | Wang X.-L.,The First Peoples Hospital Of Shangqiu
Oncology Letters | Year: 2017

The clinical value of MR diffusion tensor imaging (DTI) in grade diagnosis of gliomas was investigated. A total of 31 patients with glioma were administered 3.0T MR convention and DTI examination, with quantitative measurement of anisotropy coefficient fractional anisotropy (FA) and apparent dispersion coefficient (ADC) value, and the comparison of quantitative parameters of glioma between low- and high-grade, which was detected by Mann-Whitney U test. The receiver operation characteristic (ROC) curve was drawn to take the value of ADC and FA in tumor ROI as a critical point, to calculate the area under the curve and to confirm the diagnosis threshold value and evaluate its diagnostic efficiency. The FA value of 14 low-grade glioma cases was 139.4±81.3, with an ADC value of (1.36±0.21) ×10-3 mm2/sec. The FA value of 17 high-grade glioma cases was 103.1±41.5, with ADC value of (1.09±0.28)-3 mm2/sec; the difference between the two groups was statistically significant (P<0.05). The ADC value was taken as the critical point to judge tumor grade and draw the ROC curve; the area under the curve was 0.79. As the diagnosis threshold value, the ADC value of 1.11×10-3 mm2/sec was used to distinguish between low- and high-grade tumor with a sensitivity of 58.8% and specificity of 92.9%. The FA value was taken as a critical point to judge tumor grade and draw the ROC curve; the area under the curve was 0.62. As the diagnosis threshold value, the FA value of 178.9 was applied to distinguish between low- and high-grade tumor sensitivity of 94.1% and specificity of 35.7%. Therefore, the FA value and ADC value in DTI has an important estimated value for the pathological grade of glioma. © 2017, Spandidos Publications. All rights reserved.


Liu Y.,The First Peoples Hospital of Shangqiu | Wang F.,The First Peoples Hospital of Shangqiu | Xu P.,Shandong University
Biomedicine and Pharmacotherapy | Year: 2017

MicroRNA-590 (miR-590) shows oncogenic functions in various tumor types, but little is known about biological functions of miR-590 in lung adenocarcinoma. In this study, we observe that miR-590 is not only overexpressed in lung adenocarcinoma tissues and metastatic lymph nodes, but also significantly increased in lung adenocarcinoma cell lines. Moreover, gain-of-function and loss-of-function studies show miR-590 serve as a tumor suppressor regulating lung adenocarcinoma cells migration and invasion. Furthermore, OLFM4 is proved to as a functional target for miR-590 to regulate lung adenocarcinoma cells migration and invasion. In conclusion, miR-590 regulates lung adenocarcinoma metastasis through directly modulating functional target OLFM4. © 2016 Elsevier Masson SAS


Shi D.-F.,The Third Peoples Hospital of Shangqiu | Cao R.,The First Peoples Hospital of Shangqiu
International Eye Science | Year: 2017

AIM: To study eye position control ability in patients with different types of intermittent strabismus. METHODS: Totally 146 patients with intermittent strabismus treated in our hospital from September 2013 to April 2015 were enrolled in the study. The patients were divided into three groups according to the different types of the patients with intermittent strabismus. Fifty-five patients who had been separated from the strong type were used as part of the strong group, the basic type of 48 cases as the basic group, 44 cases of collection insufficiency patients were set up as collection insufficiency group. Visual function and postoperative eye correction of the three groups were analyzed. RESULTS: There were no differences in visual function among the three groups before operation, and the visual function of the three groups were improved after operation, and there was no difference among the three groups(P>0.05).The positive rates were 83.3%, 81.2% and 54.6% of the strong group, basic group and collection insufficiency group respectively. There was no difference between the strong group and basic group, (P>0.05).There were significant differences between the strong group, basic group with collection insufficiency group(P<0.05). CONCLUSION: Operations for intermittent external strabismus is different according to different types. The prognosis of the strong group and basic group is good and the prognosis of collection insufficiency group is poor. Copyright 2017 by the IJO Press.


Wang F.,The First Peoples Hospital of Shangqiu | Liu Y.,The First Peoples Hospital of Shangqiu | Chen Y.,Shengli Oilfield Center Hospital
Biomedicine and Pharmacotherapy | Year: 2016

Pituitary tumor transforming gene-1 (PTTG1) is a novel oncogene and overexpressed in a wide variety of human cancers. However, the clinical and prognostic significance of PTTG1 in non-small cell lung cancer (NSCLC) is still unknown. The expression status of PTTG1 in NSCLC at the publicly available GEO databases (GSE19804) was observed. The mRNA and protein expression of PTTG1 in NSCLC tissues and cell lines was detected by qRT-PCR and Western blot, and the association between PTTG1 expression and clinicopathological factors was analyzed by immunohistochemistry. In our Results, PTTG1 was one of genes overexpressed in NCSLC samples compared with paired adjacent normal lung samples in microarray data (GSE19804). PTTG1 mRNA and protein expressions were increased in NSCLC tissues and cell lines. PTTG1 protein expression was correlated with malignant status and poor prognosis of NSCLC patients. In conclusion, PTTG1 is correlated with NSCLC progression and as an independent poor prognostic factor in NSCLC patients. © 2016 Elsevier Masson SAS


Cao J.,Nanjing Agricultural University | Feng C.,The First Peoples Hospital of Shangqiu | Liu Y.,The First Peoples Hospital of Shangqiu | Wang S.,Nanjing University of Science and Technology | Liu F.,Nanjing Agricultural University
Biosensors and Bioelectronics | Year: 2014

Since many diseases are caused by pathogenic bacterial infections, accurate and rapid detection of pathogenic bacteria is in urgent need to timely apply appropriate treatments and to reduce economic costs. To end this, we designed molecular beacon-Au nanoparticle hybrid nanoprobes to improve the bacterial detection efficiency and sensitivity. Here, we show that the designed molecular beacon modified Au nanoparticles could specifically recognize synthetic DNAs targets and can readily detect targets in clinical samples. Moreover, the hybrid nanoprobes can recognize Escherichia coli within an hour at a concentration of 102cfu/ml, which is 1000-folds sensitive than using molecular beacon directly. Our results show that the molecular beacon-Au nanoparticle hybrid nanoprobes have great potential in medical and biological applications. © 2014 Elsevier B.V.


Liu Y.,Zhengzhou University | Liu Y.,The First Peoples Hospital of Shangqiu | Song L.,Zhengzhou University
International Journal of Clinical and Experimental Pathology | Year: 2015

Neuroblastoma inflicts mostly on children, and the pathogenesis remains elusive. Clinical diagnosis and therapeutic approaches are still on the incipient stage, so further understanding of the molecular and cellular mechanisms of the disease is necessary. Inflammation has been commonly regarded as a hallmark in tumorigenesis and development, and we identified a new inflammatory factor, HMGB1, is considerably increased in neuroblastoma. Our study shows that HMGB1 induces autophagy in Schwann cells through activation of TLR4, and knockdown of TLR4 obviates the HMGB1-induced autophagy. The HMGB1-induced autophagy is through classical pathway, as deficiency of Beclin 1 deprived autophagy in Schwann cells. Coculture of neuroblastoma with Schwann cells pretreated with HMGB1 promoted the proliferation of neuroblastoma cells, and if Beclin 1 is knocked down in Schwann cells, no promotion effects is observed. Taken together, our study demonstrates that HMGB1-induced autophagy in Schwann cells contributes to neuroblastoma cell proliferation, thus providing a potential therapeutic approach on neuroblastoma development.


Yang Y.-X.,The First Peoples Hospital of Shangqiu | Zhu Y.-H.,The First Peoples Hospital of Shangqiu
International Journal of Clinical and Experimental Pathology | Year: 2016

Objective: The receptor for activated protein kinase C (RACK1) has been implicated in the development and progression of several human malignancies, but its role in human laryngeal squamous cell carcinoma (LSCC) has not been investigated. Methods: We performed quantitative real-time PCR (qRT-PCR) and western blot to analyze the expression of RACK1 in LSCC tissues and their matched adjacent non-tumor tissues. Immunohistochemistry (IHC) was carried out to analyze the expression levels of RACK1 in 72 paraffin-embedded LSCC tissues. The correlation between RACK1 expression and clinicopathological features was assessed by Chi-square analysis. The survival data of LSCC patients was analyzed by the Kaplan-Meier and log rank tests. Results: Expression levels of RACK1 mRNA and protein were increased in LSCC tissues than that in adjacent non-tumor tissues (P < 0.05). RACK1 expression was significantly correlated with T classification, clinical stage and lymph nodes metastasis (P < 0.05). LSCC patients with high RACK1 expression exhibited shorter survival time compared to those with low RACk1 expression (P < 0.05). Moreover, multivariate analysis further demonstrated that upregulated expression of RACK1 was an independent prognostic factor for LSCC patients (P < 0.05). Conclusions: RACK1 play an important role in the progression of LSCC, and present as a useful prognostic marker and a potential therapeutic target for LSCC patients.


PubMed | Cangzhou Center Hospital and The First Peoples Hospital of Shangqiu
Type: | Journal: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology | Year: 2017

The establishment and maintenance of successful pregnancy mainly depends on trophoblast cells. Their dysfunction has been implicated in recurrent spontaneous abortion (RSA), a major complication of pregnancy. However, the underlying mechanisms of trophoblasts dysfunction remain unclear. DNA-damage-induced cell apoptosis has been reported to play a vital role in cell death. In this study, we identified a novel microRNA (miR-520) in RSA progression via regulating trophoblast cell apoptosis. Microarray analysis showed that miR-520 was highly expressed in villus of RSA patients. By using flow cytometry analysis, we observed miR-520 expression was correlated with human trophoblast cell apoptosis in vitro, along with decreased poly (ADP-ribose) polymerase-1 (PARP1) expression. With the analysis of clinic samples, we observed that miR-520 level was negatively correlated with PARP1 level in RSA villus. In addition, overexpression of PARP1 restored the miR-520-induced trophoblast cell apoptosis in vitro. The status of chromosome in trophoblast implied that miR-520-promoted DNA-damage-induced cell apoptosis to regulate RSA progression. These results indicated that the level of miR-520 might associate with RSA by prompting trophoblast cell apoptosis via PARP1 dependent DNA-damage pathway.


Zhu L.,The First Peoples Hospital of Shangqiu
Zhonghua nan ke xue = National journal of andrology | Year: 2013

To investigate the incidence and spermatogenesis of cryptorchid testes induced by postnatal injection of estradiol. Ninety male newborn Balb/C mice were randomly divided into an experimental (n = 60), a solvent control (n = 20) and a normal control group (n = 10). The experimental mice were again assigned to a 4-week, a 6-week, an 8-week, and a 10-week subgroup, and injected subcutaneously with 17-beta estradiol (5 microg/d) from 3 to 28, 3 to 42, 3 to 56 and 3 to 70 days after birth, respectively. The incidence of cryptorchidism and morphological changes of the testes were observed at 2 weeks after drug withdrawal. The incidence rates of cryptorchidism in the 4-, 6-, 8- and 10-week groups were 0%, 26.7%, 60% and 60%, respectively, but no cryptorchidism occurred in the solvent and normal control groups. The 4- and 6-week groups showed autonomous descent of the cryptorchid testes and recovery of spermatogenesis after drug withdrawal. The models became stable and no spermatogenesis recovery was observed after 8 weeks of continuous medication. Stable cryptorchid infertile models can be established in mice by postnatally continuous injection of estradiol for over 8 weeks.

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