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Qin X.,Anhui Medical University | Zhang Y.,Peking University | Cai Y.,Guangdong Traditional Chinese Medicine Hospital | He M.,The First Peoples Hospital of Lianyungang City | And 9 more authors.
Clinical Nutrition | Year: 2013

Background & aims: We aimed to examine the prevalence of obesity, abdominal obesity and associated factors in 17,656 Chinese hypertensive adults aged 45-75 years. Methods: A cross-sectional investigation was carried out in Lianyungang, China. Overweight or obesity was defined as a body mass index of ≥25kg/m2. Abdominal obesity was defined as a waist circumference ≥90cm for men and ≥80cm for women. Results: The prevalence of overweight or obesity and abdominal obesity was 54.4% (women 59.3% and men 46.0%) and 59.4% (women 73.8% and men 35.1%), respectively. In the multivariable logistic-regression models, higher hypertension grades and standard of living, greater red meat consumption, lower physical activity levels, and antihypertensive treatment were independently associated with overweight or obesity and abdominal obesity in both sexes. Inland residence (versus coastal) was an independent associated factor for abdominal obesity in both sexes. Furthermore, a positive family history of diabetes in both sexes, a positive family history of hypertension, men with a positive family history of coronary heart disease, and men with inland residence were all independently associated with overweight or obesity. Conclusions: We found a high prevalence of overweight or obesity and abdominal obesity in Chinese hypertensive adults, particularly in inland areas. © 2012 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism.

PubMed | The First Peoples Hospital of Lianyungang City, Shanghai JiaoTong University, Tongji University and The Affiliated Huaian Hospital of Xuzhou Medical University and The Second Peoples Hospital of Huaian
Type: | Journal: Journal of cellular physiology | Year: 2016

MicroRNAs have been used as diagnostic and prognostic biomarkers for many cancers including oral squamous cell carcinoma (OSCC). Several studies have been shown that microRNA (miRNA) play important roles during the progression of OSCC. However, the results vary largely in different studies due to different platforms and sample sizes. In this study, we systematically evaluated a large scale of miRNA profiles from current qualified OSCC samples, and further investigated the functions of genes regulated by these key miRNAs as well as the signaling pathways through which these miRNA effect carcinogenesis. Seven key miRNAs were identified, and of which 3 were significantly up-regulated, including hsa-miR-21, hsa-miR-31, hsa-miR-338, and 4 were down-regulated, namely hsa-miR-125b, hsa-miR-133a, hsa-miR-133b and hsa-miR-139. The function enrichment analysis revealed that target genes of up-regulated miRNAs were associated with cellular protein metabolic process, macromolecule metabolic process and TGF-beta pathway, while the targets of down-regulated were enriched in negative regulation of macromolecule biosynthetic process and gene expression, and p53, Long-term potentiation and adherens junction pathways. Transcription factor analysis revealed that there were 67 (51.1%) transcription factors influenced by both up and down-regulated miRNAs. To summary, 7 key miRNAs were found to play essential role in progression of OSCC, as well as the target genes and transcription factors of these miRNAs. The potential functions of these target genes identified in our study may be profitable to diagnosis and prognostic prediction of OSCC as biomarkers. This article is protected by copyright. All rights reserved.

PubMed | Subei Peoples Hospital, The First Peoples Hospital of Lianyungang City and Nanjing Medical University
Type: Journal Article | Journal: International urology and nephrology | Year: 2016

We performed this meta-analysis to evaluate the efficacy and safety of artery preserving versus artery non-preserving in laparoscopic varicocelectomy.All publications up until October 2015 were searched in PubMed, EMBASE, Ovid, Web of Science, and Cochrane library. Randomized controlled trials (RCTs) and cohort studies (CSs) that compared the difference in two operative approaches in laparoscopic varicocelectomy were included. Statistical analysis was performed using Stata version 12.0.A total of four RCTs and ten CSs involving 503 cases with artery preserving and 911 cases with artery non-preserving met our inclusion criteria. Meta-analysis showed that artery preserving had higher recurrence rate [risk ratio (RR)=2.91, 95% confidence interval (CI) 1.83-4.61; P=0.000], lower incidence of hydrocele formation (RR=0.18; 95% CI 0.08-0.42; P=0.000), and prolonged operating time [standard mean difference (SMD)=1.27; 95% CI 0.17-2.37; P=0.023], compared with artery non-preserving in laparoscopic varicocelectomy. The results were similar in postoperative catch-up growth (RR=1.00; 95% CI 0.86-1.17; P=0.985) and testicular atrophy (RR=0.36; 95% CI 0.09-1.54; P=0.169). Besides, no significant difference was found in sperm concentration, motility, and normal morphology between two groups, as well as on postoperative pregnancy rate (RR=0.95; 95% CI 0.65-1.40; P=0.809).With the advantages of less recurrence, easier operating and less time spending, and comparable results in other respects, artery non-preserving is preferable to artery preserving in laparoscopic varicocelectomy, although there is a relatively high incidence of hydrocele formation. Considering the limitation of included studies, more large-scaled RCTs are required to confirm the present findings.

Lin D.,Qingdao University | Zhao Y.,The First Peoples Hospital of Lianyungang City | Li H.,Qingdao University | Xing X.,Qingdao University
Journal of Thoracic Disease | Year: 2013

Pulmonary enteric adenocarcinoma, a rare histologic type of primary pulmonary adenocarcinoma with only 16 cases reported to date, has to be differentiated from metastatic colorectal carcinoma. Here we report a case of pulmonary enteric adenocarcinoma which shows villin immunoreactivity in the brush border of tumor cells. As a marker for gastrointestinal adenocarcinoma, villin has been rarely found positive like this pattern in pulmonary adenocarcinomas. This case suggests brush border immunoreactivity of villin is possible in some cases of pulmonary enteric adenocarcinomas. We suggest pathological practitioners pay attention to it. © Pioneer Bioscience Publishing Company.

Zhang X.,The First Peoples Hospital Of Lianyungang City | Zhang X.,Nanjing Medical University | Wang J.,Nanjing Medical University | Qian W.,Nanjing Medical University | And 4 more authors.
Neurological Research | Year: 2015

Objective: Dexmedetomidine (DEX) has been implicated in modulating the inflammatory response in central nervous system (CNS). However, the mechanism is still poorly understood. In this study, we evaluate the effects of DEX on lipopolysaccharide (LPS)-induced microglia activation and elucidate its possible signaling pathway involved in its anti-inflammatory effects.Methods: BV2 and primary microglia were pretreated with various concentrations of DEX (0.01, 0.1, 1, and 10 μM) and/or PD98059 for 1 hour, then microglia were incubated with LPS (1 mg/ml) for 24 hours. Nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression were measured by Griess reagent and real-time polymerase chain reaction. Furthermore, two intracellular signaling cascades including extracellular signal-regulated kinase (ERK1/2) and c-Jun N-terminal kinase (JNK) were investigated by western blot analysis.Results: Dexmedetomidine significantly attenuated LPS-induced NO production and iNOS expression in both BV2 cells and primary microglial cells. Lipopolysaccharide activated both ERK1/2 and JNK signal pathways; however, DEX exerted a specific inhibitory effect on ERK1/2 rather than JNK. Intriguingly, treatment of primary microglia and BV2 cells with DEX in combination with ERK1/2 inhibitor (PD98059) enhanced attenuation of LPS-induced NO production and iNOS expression.Discussion: Dexmedetomidine attenuates NO and iNOS accumulation by inhibiting extracellular signalregulated kinase (ERK) activation in both BV2 cells and primary microglial cells. © W. S. Maney & Son Ltd 2015.

PubMed | Nanjing Medical University and The First Peoples Hospital of Lianyungang City
Type: | Journal: Journal of neuroinflammation | Year: 2015

Neuroinflammation is considered a risk factor for impairments in neuronal function and cognition that arise with trauma, infection, and/or disease. IL-17A has been determined to be involved in neurodegenerative diseases such as multiple sclerosis. Recently, IL-17A has been shown to be upregulated in lipopolysaccharide(LPS)-induced systemic inflammation. This study aims to explore the role of IL-17A in LPS-induced neuroinflammation and cognitive impairment.Male Sprague-Dawley (SD) rats were injected intraperitoneally with LPS (500 g/kg), and IL-17A expression in serum and in the hippocampus was examined 6, 12, 24, and 48 h later. Then, we investigated whether IL-17A-neutralizing antibodies (IL-17A Abs, 1 mg/kg) prevented neuroinflammation and memory dysfunction in aged rats that received LPS (500 g/kg) injection. In addition, the effect of IL-17A on microglial activation in vitro was determined using ELISA and immunofluorescence.LPS injection increased the expression of IL-17A in serum and in the hippocampus. IL-17A Abs improved LPS-induced memory impairment. In addition, IL-17A Abs prevented the LPS-induced expression of TNF-, IL-6 and inflammatory proteins, and of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as the activation of microglia in the brain. IL-17A Abs also inhibited the expression of amyloid precursor protein (APP) and BACE1 and increased the expression of the synaptic marker PSD95 in the aged rats treated with LPS. In an in vitro study, we found that recombinant IL-17A could simulate microglial activation and increase production of pro-inflammatory cytokines.Taken together, our results suggest that IL-17A was involved in LPS-induced neuroinflammation and cognitive impairment in aged rats via microglial activation. Anti-IL-17A may represent a new therapeutic strategy for the treatment of endotoxemia-induced neuroinflammation and cognitive dysfunction.

To identify the effect of dexmedetomidine added to ropivacaine on ultrasound-guided transversus abdominis plane block for postoperative analgesia after abdominal hysterectomy surgery.Sixty patients were enrolled in the study and divided into two groups. Bilateral 20 mL 0.3% ropivacaine and 2 mL 0.9% normal saline for ropivacaine group (group R), and bilateral 20 mL 0.3% ropivacaine and 2 mL dexmedetomidine (0.5 g/kg) for dexmedetomidine group (group RD). Visual Analogue Scale (VAS) pain scores, frequency of PCA pressed, sufentanil consumption, and postoperative nausea and vomiting (PONV) were recorded.There was no significant difference in VAS pain scores between the two groups at different time intervals (P>0.05). Compared with group RD, the frequency of PCA pressed and integrated consumption of sufentanil were significantly higher in Group R at 0-2, 2-4, 4-6, 6-8, time intervals (P<0.05) except for 8-12,12-24 time intervals (P>0.05), and the 24h total sufentanil consumption and frequency of PCA pressed were also higher in group R (63.910.0 vs. 51.89.1, 8.31.7 vs. 5.41.6) (P<0.05). There was no difference in PONV between the two groups (P>0.05).Ultrasound-guided TAP block could be as an effective component of multimodal postoperative analgesic regimen; adding dexmedetomidine to ropivacaine in TAP block potentiated the analgesic properties of ropivacaine, reduced sufentanil consumption and provided better pain control after abdominal hysterectomy surgery.

PubMed | The First Peoples Hospital of Lianyungang City
Type: Journal Article | Journal: Turkish neurosurgery | Year: 2016

To determine whether specific clinical and radiographic factors predispose arteriovenous malformations (AVMs) presenting with seizure and to predict the seizure risk for individual AVM patients.Clinical features and cerebral angiograms of consecutive 45 unruptured AVM patients who were diagnosed in our center in a 2-year period were reviewed. Patient data (analysis cohort) was used to determine risk factors for seizure and to construct epileptogenic AVM groups. These risk groups were tested with the second half of the patient data (test cohort).Among 45 unruptured AVMs (47.9%), initial seizures occurred in 20 unruptured AVMs (44.4%). Two of these 20 patients had a bleed in 117 patient-years for an annual bleed rate of 1.7%. There was no significant difference in hemorrhagic risk between epileptogenic AVM and asymptomatic AVM (P=0.918). Multivariate analysis revealed 2 factors associated with seizure: frontal and temporal AVM locations (P < 0.001) and a compact AVM morphology (P=0.003).Analysis of a group of unruptured AVMs demonstrated that epileptogenic AVMs have an annual hemorrhage risk similar to that of the asymptomatic AVMs. Frontal and temporal AVM locations and a compact AVM morphology were significantly associated with epileptogenic AVMs.

PubMed | The First Peoples Hospital of Lianyungang City
Type: Comparative Study | Journal: The Journal of surgical research | Year: 2015

Previous studies have demonstrated that ultrasonographic measurement of the inferior vena cava diameter is a useful tool for the evaluation of intravascular volume status in preoperative patients. However, ultrasonographic measurement of inferior vena cava diameter could be limited by factors including obesity, bowel gas, or complex abdominal wounds. Our study sought to determine whether subclavian vein (SCV) diameter measured by ultrasound correlate with central venous pressure (CVP), as another indicator of intravascular volume status in patients undergoing gastrointestinal surgery.Forty patients (American Society of Anesthesiologists I-II) who underwent elective gastrointestinal surgery and 40 healthy volunteers were enrolled in the study. In the patient group, SCV diameters, during both expiration (dSCVe) and inspiration (dSCVi), were measured with ultrasonography before and after fluid resuscitation. Volunteer baseline measurements were conducted without liquid therapy and the subsequent measurement.Forty patients (mean age 46 y; 40% female) and 40 volunteers (mean age 43 y; 45% female) underwent SCV sonographic measurements. The average diameters of the SCVe and SCVi in hypovolemic patients (0.68, 0.48 cm) were significantly lower as compared with the SCVe and SCVi diameters of healthy volunteers (0.92, 0.73 cm), whereas the SCV-collapsibility index (0.35) was higher in the hypovolemic patients as compared with the healthy volunteers (0.20). After fluid resuscitation, the SCVe and SCVi diameters in hypovolemic patients (0.88, 0.67 cm) significantly increased, whereas the SCV-collapsibility index decreased (0.23). The pre-SCVe and the post-SCVe were closely correlated to the CVP (R = 0.612 and R = 0.547, respectively). Similarly, the pre-SCVi and the post-SCVi were correlated to the CVP (R = 0.452 and R = 0.507, respectively).SCV diameter is consistently low in patients undergoing gastrointestinal surgery as compared with healthy subjects. Measuring the SCV diameter maybe an important addition to the ultrasonographic evaluation of hypovolemia and other potentially volume-depleted patients.

PubMed | The Third Peoples Hospital of Lianyungang City and The First Peoples Hospital of Lianyungang City
Type: Journal Article | Journal: Molecular medicine reports | Year: 2014

The objective of the present study was to search for Msh homeobox 1 (MSX1), paired box gene 9 (PAX9), ectodysplasinA (EDA) and axis inhibition protein 2 (AXIN2) variants in a family with isolated oligodontia and analyse the pathogenesis of mutations that result in oligodontia phenotypes. Members of a single family (but of different descent) with oligodontia and unrelated healthy controls were enrolled in our study. Genomic DNA was isolated from blood samples. Mutation analysis was performed by amplifying MSX1, PAX9, EDA and AXIN2 exons as well as their exonintron boundaries and sequencing the products. DNA sequencing of the AXIN2 gene revealed three mutations in the two patients with oligodontia: a homozygotic silent mutation c.1365A>G (p.Pro455=) in exon 3, two c.956+16A>G mutations (II1: homozygosis; III1: heterozygosis) and c.1200+71A>G (homozygosis) in the intron, which possibly contributed to structural and functional changes in proteins. The heterozygotic mutations c.1365A>G and c.1200+71A>G were identified in the probands mother (II2). No mutations were detected in the MSX1, PAX9 and EDA genes of oligodontia patients. The findings suggest that the c.956+16A>G, c.1365A>G and c.1200+71A>G mutations of AXIN2 may be responsible for the oligodontia phenotype in this family, but these findings require further study.

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